Status
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Gene information
General information
TC011135 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4177470..4179657
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011135
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01811

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011135 RA
      MLLPILYAV STLDALAFA LVITTINPY AFSLGGTQF 
      YLGVLGSLA ALINLFWNP IVGSLSDSL GRKKILLLC 
      LAFVFVGNL LATLFSSLT VLFISRILS TFGTQIAIL 
      IKSLLDDKI KTKETKMAA IYRMQAIGG IAFISGALI 
      GGHLSEHAN GFRITFALI STMVAVNFA LICLIPDVH 
      TTEKKAVPT LQIATELKN AAANLTKVN WPKYWDLFT 
      MKLIVDFVM NVMQFNLGL ILQQRFGVS GKLFGYVFA 
      IASVVSIAT SLAMSKAQK SLYKNDISG LQRLKHGFL 
      LLMLACLGL GLSPNVILF CLFLMPTSV TRVLFESTF 
      TEIILARAS PQEKGSIMG TFETTLSLG GLTAPLISG 
      IVSDFWGEM YCILMCAVP LVLGTGLIY LQKSKTQ
    CDS sequence
    • >TC011135 RA
      atgctttta ccgatttta tacgccgtc tctaccttg 
      gacgctctt gcttttgca ttggtcata actacgata 
      aatccctat gccttttca ttaggagga acccaattt 
      tatttagga gttttaggc tcactagca gccttaatt 
      aacctattt tggaaccct attgtgggg agtttaagt 
      gacagtctt ggacgcaaa aaaatcctc ctactatgc 
      ctcgccttt gtcttcgtt ggaaattta ctagcgaca 
      ctattttcg tccctaact gtacttttc atctcgcgg 
      attttatca acatttggg acccaaatc gcaatcctg 
      atcaagtcg ctccttgac gacaaaatc aaaacaaaa 
      gaaaccaaa atggcagcg atatatcga atgcaagcg 
      ataggcggc attgctttc atttccggc gccctgatt 
      ggtgggcat ttgagtgag catgccaac ggtttcagg 
      atcacattt gccttgata tctaccatg gttgcggtt 
      aatttcgca ttgatttgc ctgatccca gacgtccac 
      accacagaa aaaaaagca gtgccaaca ctgcaaatt 
      gcaacggaa ctgaaaaac gctgctgcc aacctaacc 
      aaagttaac tggccgaaa tattgggac ttgttcact 
      atgaaacta atcgttgat tttgtaatg aacgtaatg 
      cagttcaat ttgggtctg attttgcag caacgtttc 
      ggcgtcagt ggaaaactc ttcggttac gttttcgct 
      atagcgagt gttgtgtca attgctacg agtctagcg 
      atgagcaaa gcgcagaaa tctttgtat aaaaatgat 
      atttctggg ttacagaga ctaaaacat ggatttttg 
      ctcctaatg ttggcttgt ttggggctc ggtttgagc 
      cccaatgta attttattt tgtttgttt cttatgcca 
      acgtctgtg accagagtt ttgttcgag tcgactttt 
      accgagatt attttggca agggcttcg ccccaggaa 
      aaggggagt attatgggg acgttcgag acgacgctg 
      tctctaggg gggctcact gcgcccctg atttcaggg 
      attgtgtcc gatttttgg ggcgaaatg tactgtatt 
      ttaatgtgt gcggtgccc cttgtgttg ggaacaggt 
      ttaatttat ttgcagaag agcaaaacg caatag
    mRNA sequence
    • >TC011135 RA
      ttattgaat gaacatgct tttaccgat tttatacgc 
      cgtctctac cttggacgc tcttgcttt tgcattggt 
      cataactac gataaatcc ctatgcctt ttcattagg 
      aggaaccca attttattt aggagtttt aggctcact 
      agcagcctt aattaacct attttggaa ccctattgt 
      ggggagttt aagtgacag tcttggacg caaaaaaat 
      cctcctact atgcctcgc ctttgtctt cgttggaaa 
      tttactagc gacactatt ttcgtccct aactgtact 
      tttcatctc gcggatttt atcaacatt tgggaccca 
      aatcgcaat cctgatcaa gtcgctcct tgacgacaa 
      aatcaaaac aaaagaaac caaaatggc agcgatata 
      tcgaatgca agcgatagg cggcattgc tttcatttc 
      cggcgccct gattggtgg gcatttgag tgagcatgc 
      caacggttt caggatcac atttgcctt gatatctac 
      catggttgc ggttaattt cgcattgat ttgcctgat 
      cccagacgt ccacaccac agaaaaaaa agcagtgcc 
      aacactgca aattgcaac ggaactgaa aaacgctgc 
      tgccaacct aaccaaagt taactggcc gaaatattg 
      ggacttgtt cactatgaa actaatcgt tgattttgt 
      aatgaacgt aatgcagtt caatttggg tctgatttt 
      gcagcaacg tttcggcgt cagtggaaa actcttcgg 
      ttacgtttt cgctatagc gagtgttgt gtcaattgc 
      tacgagtct agcgatgag caaagcgca gaaatcttt 
      gtataaaaa tgatatttc tgggttaca gagactaaa 
      acatggatt tttgctcct aatgttggc ttgtttggg 
      gctcggttt gagccccaa tgtaatttt attttgttt 
      gtttcttat gccaacgtc tgtgaccag agttttgtt 
      cgagtcgac ttttaccga gattatttt ggcaagggc 
      ttcgcccca ggaaaaggg gagtattat ggggacgtt 
      cgagacgac gctgtctct aggggggct cactgcgcc 
      cctgatttc agggattgt gtccgattt ttggggcga 
      aatgtactg tattttaat gtgtgcggt gccccttgt 
      gttgggaac aggtttaat ttatttgca gaagagcaa 
      aacgcaata gttgtatca acaacttaa ctttttatt 
      aaacaattt taaataaaa caagtgttt taattaccc 
      ggcactgcg ttccaaggc cttgatccg gtcggcttc 
      ttgtttggc tttgatttt ttgaatggc atcgaggaa 
      ttctcgttg ttgggtggt gttttcagg ggggtagag 
      ctcccgttt tgtcttcac gacccattc ttcgaataa 
      ttccggttg gttgaagta atgaaacat tgccaccgg 
      gaaggttat ataaagggc catttttcc aatttcgag 
      acgccaact gcccattgt ggctattat tcgtggttt 
      ttgcgaaga aaatatgca caaaaagtg gggttaagt 
      agctttacg tcaaaattg gaggaaact gccataggc 
      gactccgac tcgtcggta aaattgtaa aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY