Status
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Gene information
General information
TC031382
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011129,TC011130
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4219869..4230377
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031382
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01810

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    gut mostly remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 6

    wings bent - Number of animals showing the phenotype: 6

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031382 RA
      MKKTDFEES GAPPVSVLR RIKTITQCF DRPMVSQIT 
      QNGQFWVLN NHIKASERF KCHESVTIT TQGDFMFLE 
      NLPILVERW KGPISVAVY APGSDFKRT IDSIRYLRH 
      CDSPLIAKF VTFHIFFDA EHVPEKNFQ PEEGFDCDK 
      KNPPYHTKT TTYKTSQNL LYPINVARN IARKMAQTH 
      FILPLDIEL FPTPDFIPK FLAMIARNQ PSLNPRVFP 
      LPVFEIESG QKLPENKTQ LRELLSRKL AIPFHENIC 
      AMCHNIPDL DEWVQLNET KNLAVFRTT KRMGEYRSW 
      EPFYVGTHS EPYFHEGLS WEGGSDKRA QGYVMCLLD 
      YDFMVLDNA FLVHKPGIK KNTYDEGRF KYQEQTNEF 
      LSGRFLDEM NFLYNFSII FFVLKLAPP CTGSCTRAL 
      RGGHAQVNP SALRPLLSP PPVSEARRR PTQPPSAVA 
      RSTHTHQKP TEMDAFDTW RTYATICRL CLQKDGFML 
      GIFNNVQGK DKSIYKKII DCTALQISH DDGLPNVIC 
      HRCLYKIEF CLEFRKLCF MSDATLRQL TAAITNETS 
      ETDCGPAPQ LPYLEQLAN GNEEDVVMV VDPSALDYE 
      SSYESENER MSDAENVEE GGKNVSMCR YCDHAFTDK 
      LECEEHENT AHDTNVPYT CSLCEMNFA DRMQYSAHL 
      KSVHQNDKP YNCPQCERT FARRSDLRK HTVVHTGIK 
      PYTCTVCSK SFSRNTNLS KHMRIHSGQ KPFVCPKCP 
      KTFFSKGDL TRHAIIHSG QKPFSCNFC HLSFGRKDK 
      LLRHEKRHF PQENMEDKS QELQVMRDA MEDYYKEEE 
      NMVISVDPF QHNIKTENQ SETDLGEVP RDLPQIPAH 
      ITGDSFGEE RKRYSCDSC PKTFSNPEN MRYHHATHS 
      GVRPHACSV CKKTFIRKR ELDRHFATH TGMKPFKCV 
      KCSKSFGRK DKLVRHMRI HDVNKEHSC TMCAATFNR 
      RDGLLQHIK THVKNEQS
    CDS sequence
    • >TC031382 RA
      atgaaaaaa actgacttt gaagagtcc ggtgctccc 
      cctgtttcg gttttacga aggataaag actatcacc 
      cagtgtttt gatcggccc atggtgagc caaattacg 
      caaaacggc caattttgg gtcttgaac aaccacata 
      aaggcctca gaacggttc aaatgccat gaaagtgtc 
      acaataaca acgcaaggc gatttcatg ttccttgaa 
      aacttgccc atcttggtg gagagatgg aagggaccc 
      atcagtgtg gccgtctac gcccctgga agcgatttt 
      aaacgcacc atcgactcc attcgctat ctacgccac 
      tgtgatagc ccccttatt gcgaagttt gtcacgttt 
      catattttt ttcgatgcg gaacatgtt ccagaaaag 
      aatttccag ccggaagaa ggcttcgat tgtgacaaa 
      aaaaatccc ccatatcac accaaaaca accacttac 
      aaaacctcc caaaacttg ctttatcct atcaacgtg 
      gcccgaaat atagcgcga aaaatggcc caaacccac 
      ttcatcctc cccttggac atcgaactg ttcccgact 
      cccgacttc atcccgaaa ttcctggca atgatcgcc 
      cgcaatcaa ccgtccctc aacccccgg gtcttccct 
      ttgccagtt ttcgaaatc gaatccggg caaaaactc 
      cccgaaaat aaaacccaa ctccgggaa ctgctctcc 
      agaaaatta gcaattcct ttccacgaa aatatttgt 
      gcaatgtgt cacaacatc ccggatttg gacgaatgg 
      gtgcaactg aacgaaacg aaaaatttg gcggttttt 
      cggacaacg aagaggatg ggagagtat cggtcgtgg 
      gaaccgttt tatgttggc actcattct gagccctat 
      tttcacgag ggattgtca tgggagggc gggagtgat 
      aaaagggct caagggtac gtcatgtgc ctgcttgat 
      tacgacttc atggtactt gataatgca ttcttggtg 
      cataaacca gggattaag aaaaacact tatgacgag 
      ggaaggttt aaataccag gagcaaacc aacgagttc 
      ctcagtgga cgctttttg gatgaaatg aatttcttg 
      tacaatttc agtataatt tttttcgtt ttgaagttg 
      gcgccaccg tgcaccggc agctgcacc cgggcgctc 
      cgagggggc cacgcgcaa gtcaaccca agcgccctt 
      cgtccgctc ctcagtcca cctcctgtg agtgaagcg 
      aggcggcgc cccacgcag ccaccaagc gccgttgcc 
      aggagcact cacacccat caaaagccc accgaaatg 
      gacgctttc gacacctgg cgcacgtac gccaccatc 
      tgcagactc tgcctccag aaggacggc ttcatgttg 
      ggcatcttc aacaacgtg caaggcaaa gacaagagc 
      atctacaag aagatcatc gattgcacc gccttgcag 
      atcagccat gacgatggc cttccaaac gtcatctgt 
      caccgctgc ctttacaaa atcgaattc tgtctcgaa 
      ttccgtaaa ttatgtttc atgtcggac gccaccctc 
      cgtcagctg acggcggcc ataaccaat gaaacgtcc 
      gaaacggac tgtggtccc gccccccaa cttccctac 
      ctcgagcaa ctggccaat ggcaacgaa gaggatgtt 
      gtcatggtg gtcgatccg tccgccctc gactacgaa 
      tcgtcgtac gaaagcgaa aatgagcga atgagcgat 
      gcggaaaat gtcgaagag ggcgggaaa aacgtatca 
      atgtgtcgg tactgtgac cacgccttt accgacaag 
      ctggagtgt gaagaacac gagaacacc gcacatgat 
      acaaacgtt ccgtacact tgtagtctg tgcgagatg 
      aattttgcc gaccgaatg caatattcg gcccacttg 
      aagtcggtc catcagaat gataagccg tataattgc 
      ccacagtgt gaacggacg ttcgcccga cggtcggac 
      ttgaggaaa cacactgta gtacatacg gggatcaag 
      ccgtacact tgtacagtg tgttcgaag tcgttttca 
      cgaaacacg aatttatcg aaacatatg cgcattcat 
      tcgggacag aaaccgttc gtttgtccg aaatgtccg 
      aaaacgttt ttctccaaa ggcgacttg acgcgacat 
      gcgatcata cattcgggc cagaaaccg ttcagttgt 
      aacttttgc cacctgagt ttcggacgt aaagataaa 
      ctcctcagg catgaaaag cgccatttt ccgcaagag 
      aacatggag gataagagt caggagttg caagtgatg 
      cgtgatgcc atggaggat tactacaag gaggaggag 
      aacatggtg ataagtgta gatccgttc cagcacaat 
      atcaagacc gagaatcag tcggagacc gatttgggg 
      gaagtaccg agggactta ccgcaaatt ccagcacat 
      atcactgga gatagtttc ggggaagaa cgtaaacgg 
      tattcgtgt gattcgtgt ccgaagacg ttctccaat 
      ccggagaat atgaggtat catcatgcc actcattca 
      ggggttaga ccgcacgcg tgttctgtg tgtaagaag 
      acttttata aggaagagg gagttggac cggcatttt 
      gccacgcac actgggatg aagccgttc aagtgtgtg 
      aagtgtagt aagagtttt gggcggaag gataagttg 
      gtgcgacat atgaggata catgatgtt aataaggaa 
      cattcgtgt acgatgtgt gcggcgacg tttaatcga 
      agggatgga cttttgcag catattaaa acgcacgtt 
      aagaatgag cagagttag
    mRNA sequence
    • >TC031382 RA
      tcaaaactt gtccaaaaa cagctattt agatgcctg 
      tgttcaggt gccgtttta gaaagcagc attttttgt 
      ggctccttg tattagttc tagttttaa taattgtaa 
      ttcatcaaa ttattgccc gaatgaaaa aaactgact 
      ttgaagagt ccggtgctc cccctgttt cggttttac 
      gaaggataa agactatca cccagtgtt ttgatcggc 
      ccatggtga gccaaatta cgcaaaacg gccaatttt 
      gggtcttga acaaccaca taaaggcct cagaacggt 
      tcaaatgcc atgaaagtg tcacaataa caacgcaag 
      gcgatttca tgttccttg aaaacttgc ccatcttgg 
      tggagagat ggaagggac ccatcagtg tggccgtct 
      acgcccctg gaagcgatt ttaaacgca ccatcgact 
      ccattcgct atctacgcc actgtgata gccccctta 
      ttgcgaagt ttgtcacgt ttcatattt ttttcgatg 
      cggaacatg ttccagaaa agaatttcc agccggaag 
      aaggcttcg attgtgaca aaaaaaatc ccccatatc 
      acaccaaaa caaccactt acaaaacct cccaaaact 
      tgctttatc ctatcaacg tggcccgaa atatagcgc 
      gaaaaatgg cccaaaccc acttcatcc tccccttgg 
      acatcgaac tgttcccga ctcccgact tcatcccga 
      aattcctgg caatgatcg cccgcaatc aaccgtccc 
      tcaaccccc gggtcttcc ctttgccag ttttcgaaa 
      tcgaatccg ggcaaaaac tccccgaaa ataaaaccc 
      aactccggg aactgctct ccagaaaat tagcaattc 
      ctttccacg aaaatattt gtgcaatgt gtcacaaca 
      tcccggatt tggacgaat gggtgcaac tgaacgaaa 
      cgaaaaatt tggcggttt ttcggacaa cgaagagga 
      tgggagagt atcggtcgt gggaaccgt tttatgttg 
      gcactcatt ctgagccct attttcacg agggattgt 
      catgggagg gcgggagtg ataaaaggg ctcaagggt 
      acgtcatgt gcctgcttg attacgact tcatggtac 
      ttgataatg cattcttgg tgcataaac cagggatta 
      agaaaaaca cttatgacg agggaaggt ttaaatacc 
      aggagcaaa ccaacgagt tcctcagtg gacgctttt 
      tggatgaaa tgaatttct tgtacaatt tcagtataa 
      tttttttcg ttttgaagt tggcgccac cgtgcaccg 
      gcagctgca cccgggcgc tccgagggg gccacgcgc 
      aagtcaacc caagcgccc ttcgtccgc tcctcagtc 
      cacctcctg tgagtgaag cgaggcggc gccccacgc 
      agccaccaa gcgccgttg ccaggagca ctcacaccc 
      atcaaaagc ccaccgaaa tggacgctt tcgacacct 
      ggcgcacgt acgccacca tctgcagac tctgcctcc 
      agaaggacg gcttcatgt tgggcatct tcaacaacg 
      tgcaaggca aagacaaga gcatctaca agaagatca 
      tcgattgca ccgccttgc agatcagcc atgacgatg 
      gccttccaa acgtcatct gtcaccgct gcctttaca 
      aaatcgaat tctgtctcg aattccgta aattatgtt 
      tcatgtcgg acgccaccc tccgtcagc tgacggcgg 
      ccataacca atgaaacgt ccgaaacgg actgtggtc 
      ccgcccccc aacttccct acctcgagc aactggcca 
      atggcaacg aagaggatg ttgtcatgg tggtcgatc 
      cgtccgccc tcgactacg aatcgtcgt acgaaagcg 
      aaaatgagc gaatgagcg atgcggaaa atgtcgaag 
      agggcggga aaaacgtat caatgtgtc ggtactgtg 
      accacgcct ttaccgaca agctggagt gtgaagaac 
      acgagaaca ccgcacatg atacaaacg ttccgtaca 
      cttgtagtc tgtgcgaga tgaattttg ccgaccgaa 
      tgcaatatt cggcccact tgaagtcgg tccatcaga 
      atgataagc cgtataatt gcccacagt gtgaacgga 
      cgttcgccc gacggtcgg acttgagga aacacactg 
      tagtacata cggggatca agccgtaca cttgtacag 
      tgtgttcga agtcgtttt cacgaaaca cgaatttat 
      cgaaacata tgcgcattc attcgggac agaaaccgt 
      tcgtttgtc cgaaatgtc cgaaaacgt ttttctcca 
      aaggcgact tgacgcgac atgcgatca tacattcgg 
      gccagaaac cgttcagtt gtaactttt gccacctga 
      gtttcggac gtaaagata aactcctca ggcatgaaa 
      agcgccatt ttccgcaag agaacatgg aggataaga 
      gtcaggagt tgcaagtga tgcgtgatg ccatggagg 
      attactaca aggaggagg agaacatgg tgataagtg 
      tagatccgt tccagcaca atatcaaga ccgagaatc 
      agtcggaga ccgatttgg gggaagtac cgagggact 
      taccgcaaa ttccagcac atatcactg gagatagtt 
      tcggggaag aacgtaaac ggtattcgt gtgattcgt 
      gtccgaaga cgttctcca atccggaga atatgaggt 
      atcatcatg ccactcatt caggggtta gaccgcacg 
      cgtgttctg tgtgtaaga agactttta taaggaaga 
      gggagttgg accggcatt ttgccacgc acactggga 
      tgaagccgt tcaagtgtg tgaagtgta gtaagagtt 
      ttgggcgga aggataagt tggtgcgac atatgagga 
      tacatgatg ttaataagg aacattcgt gtacgatgt 
      gtgcggcga cgtttaatc gaagggatg gacttttgc 
      agcatatta aaacgcacg ttaagaatg agcagagtt 
      aggttgtgg tggttgttt tgcactgaa taaagtatt 
      tttgcattc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY