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Gene information
General information
TC011128 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid74: 1795809..1815732
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011128
    iBeetle screen iB_01809
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011128 RA
      MSKKVKNAI KINQPLLRP FDHLACPSI LTSGIDLQA 
      AKSKMYSNR KAFAVKCTL GIIAIYFFV HVLTTSRAT 
      EAIRELQQS GRNLAALEP MDPLDEATK APSTTPQSV 
      VNVFERVKE ITRCLDRPM APRVQQRGD YWVLYNYVM 
      AEKKFKCQE SVTYTTHAD YSFMDNLLP LLDRWRGPV 
      SLALHAPGT DFKNTLESI AYLRDCTEG LVRELVTFH 
      VYFSTKHVP KEVPRHNHV FTELYNCSL PPPFLNVSS 
      SQMYKNQKK LLYPVNVGR NVARETAQT HYILPSDIE 
      LYPSPNIIP QFLAMIAEN RGPLLSKNP KVFPLHLFE 
      VSANQQVPE SKTKLIEML NNGTAVSFH KKLCPGCHN 
      IPKAKEWQM AKETDDLKV FHVGKRNGR YIHWEPIFI 
      GTHADPLYD ERLSWEGKS DKMTQGYAL CVLDYDFLI 
      LNNAFLVHR PGIKVYKKD QKRAMLAAK TNQLIKKII 
      FPELKVLYG VKKGCAV
    • >TC011128 RB
      MYSNRKAFA VKCTLGIIA IYFFVHVLT TSRATEAIR 
      ELQQSGRNL AALEPMDPL DEATKAPST TPQSVVNVF 
      ERVKEITRC LDRPMAPRV QQRGDYWVL YNYVMAEKK 
      FKCQESVTY TTHADYSFM DNLLPLLDR WRGPVSLAL 
      HAPGTDFKN TLESIAYLR DCTEGLVRE LVTFHVYFS 
      TKHVPKEVP RHNHVFTEL YNCSLPPPF LNVSSSQMY 
      KNQKKLLYP VNVGRNVAR ETAQTHYIL PSDIELYPS 
      PNIIPQFLA MIAENRGPL LSKNPKVFP LHLFEVSAN 
      QQVPESKTK LIEMLNNGT AVSFHKKLC PGCHNIPKA 
      KEWQMAKET DDLKVFHVG KRNGRYIHW EPIFIGTHA 
      DPLYDERLS WEGKSDKMT QGYALCVLD YDFLILNNA 
      FLVHRPGIK VYKKDQKRA MLAAKTNQL IKKIIFPEL 
      KVLYGVKKG CAV
    CDS sequence
    • >TC011128 RA
      atgtcgaaa aaagtgaaa aacgcaatc aaaatcaat 
      caacctctt ctccggccc tttgaccat ctggcatgt 
      ccctccatt cttacctcc ggcatcgac ctccaggcc 
      gcaaaaagt aaaatgtac agcaaccgc aaagctttc 
      gccgttaaa tgcacactg ggcatcatc gccatctac 
      ttcttcgtc catgtgttg accaccagt agggccacg 
      gaggccatc cgcgagttg caacagtcg gggcgtaat 
      ctcgcagcc ctcgagccc atggacccg cttgatgaa 
      gccaccaag gcaccttcc actacacct cagagcgtc 
      gtcaacgtg tttgaaagg gtgaaggag atcaccagg 
      tgtctggac cggcctatg gcccccagg gtgcagcaa 
      aggggcgac tattgggtg ctctacaac tacgtcatg 
      gctgaaaaa aagtttaaa tgtcaagag agtgtgacg 
      tatacgacg catgccgat tattccttc atggacaat 
      ctgttgcct ttgctggac aggtggagg gggcccgtg 
      agtctggcc ctgcatgcg ccagggacg gattttaaa 
      aacacgtta gagtccatc gcctatttg agggactgt 
      acggagggg ctggtccgg gagttggtc acgtttcat 
      gtgtatttt agcacgaaa catgtaccg aaagaagtt 
      cctcgccac aaccacgtc ttcaccgag ctttacaac 
      tgctccctt cctccacct tttcttaac gtttcttcg 
      agtcaaatg tacaaaaac cagaagaag cttctgtat 
      ccggtcaat gtgggtcga aacgttgca cgcgaaacg 
      gcccaaact cactacatt cttccatca gacatcgaa 
      ctctatccc agtcctaat atcattccg caatttctt 
      gcaatgatt gccgaaaat cgcggtcct ttgctcagt 
      aaaaatccc aaagtgttt cctcttcat ttgttcgaa 
      gtgagtgcc aatcagcaa gtacccgaa agcaagaca 
      aagttgatt gaaatgttg aataatggt actgccgta 
      tcgtttcat aaaaaactt tgtcccggt tgtcataat 
      ataccaaaa gcgaaagag tggcaaatg gcgaaagaa 
      accgatgac ctgaaggtg tttcacgtt gggaaacga 
      aacggaagg tatatacat tgggagccg atctttata 
      ggcacgcat gccgatcct ttatacgac gaaaggttg 
      agttgggag ggaaaaagc gataagatg actcaggga 
      tacgctctg tgcgtgttg gattacgac tttctcatc 
      ctcaacaac gcctttctc gtccaccga ccgggcata 
      aaagtctac aaaaaagac caaaagcgt gcaatgttg 
      gccgcaaaa accaatcaa ctcatcaaa aagataatt 
      tttccagaa ctgaaagtg ttgtatggg gtgaaaaaa 
      ggctgtgct gtgtaa
    • >TC011128 RB
      atgtacagc aaccgcaaa gctttcgcc gttaaatgc 
      acactgggc atcatcgcc atctacttc ttcgtccat 
      gtgttgacc accagtagg gccacggag gccatccgc 
      gagttgcaa cagtcgggg cgtaatctc gcagccctc 
      gagcccatg gacccgctt gatgaagcc accaaggca 
      ccttccact acacctcag agcgtcgtc aacgtgttt 
      gaaagggtg aaggagatc accaggtgt ctggaccgg 
      cctatggcc cccagggtg cagcaaagg ggcgactat 
      tgggtgctc tacaactac gtcatggct gaaaaaaag 
      tttaaatgt caagagagt gtgacgtat acgacgcat 
      gccgattat tccttcatg gacaatctg ttgcctttg 
      ctggacagg tggaggggg cccgtgagt ctggccctg 
      catgcgcca gggacggat tttaaaaac acgttagag 
      tccatcgcc tatttgagg gactgtacg gaggggctg 
      gtccgggag ttggtcacg tttcatgtg tattttagc 
      acgaaacat gtaccgaaa gaagttcct cgccacaac 
      cacgtcttc accgagctt tacaactgc tcccttcct 
      ccacctttt cttaacgtt tcttcgagt caaatgtac 
      aaaaaccag aagaagctt ctgtatccg gtcaatgtg 
      ggtcgaaac gttgcacgc gaaacggcc caaactcac 
      tacattctt ccatcagac atcgaactc tatcccagt 
      cctaatatc attccgcaa tttcttgca atgattgcc 
      gaaaatcgc ggtcctttg ctcagtaaa aatcccaaa 
      gtgtttcct cttcatttg ttcgaagtg agtgccaat 
      cagcaagta cccgaaagc aagacaaag ttgattgaa 
      atgttgaat aatggtact gccgtatcg tttcataaa 
      aaactttgt cccggttgt cataatata ccaaaagcg 
      aaagagtgg caaatggcg aaagaaacc gatgacctg 
      aaggtgttt cacgttggg aaacgaaac ggaaggtat 
      atacattgg gagccgatc tttataggc acgcatgcc 
      gatccttta tacgacgaa aggttgagt tgggaggga 
      aaaagcgat aagatgact cagggatac gctctgtgc 
      gtgttggat tacgacttt ctcatcctc aacaacgcc 
      tttctcgtc caccgaccg ggcataaaa gtctacaaa 
      aaagaccaa aagcgtgca atgttggcc gcaaaaacc 
      aatcaactc atcaaaaag ataattttt ccagaactg 
      aaagtgttg tatggggtg aaaaaaggc tgtgctgtg 
      taa
    mRNA sequence
    • >TC011128 RA
      gactgctgg tgagtggtt cagtctcca gaagcatct 
      tgtgtcggt tacagcagt cgaaaacca gcaaaaaat 
      tgaacgtcg tttctcaca atgtcgaaa aaagtgaaa 
      aacgcaatc aaaatcaat caacctctt ctccggccc 
      tttgaccat ctggcatgt ccctccatt cttacctcc 
      ggcatcgac ctccaggcc gcaaaaagt aaaatgtac 
      agcaaccgc aaagctttc gccgttaaa tgcacactg 
      ggcatcatc gccatctac ttcttcgtc catgtgttg 
      accaccagt agggccacg gaggccatc cgcgagttg 
      caacagtcg gggcgtaat ctcgcagcc ctcgagccc 
      atggacccg cttgatgaa gccaccaag gcaccttcc 
      actacacct cagagcgtc gtcaacgtg tttgaaagg 
      gtgaaggag atcaccagg tgtctggac cggcctatg 
      gcccccagg gtgcagcaa aggggcgac tattgggtg 
      ctctacaac tacgtcatg gctgaaaaa aagtttaaa 
      tgtcaagag agtgtgacg tatacgacg catgccgat 
      tattccttc atggacaat ctgttgcct ttgctggac 
      aggtggagg gggcccgtg agtctggcc ctgcatgcg 
      ccagggacg gattttaaa aacacgtta gagtccatc 
      gcctatttg agggactgt acggagggg ctggtccgg 
      gagttggtc acgtttcat gtgtatttt agcacgaaa 
      catgtaccg aaagaagtt cctcgccac aaccacgtc 
      ttcaccgag ctttacaac tgctccctt cctccacct 
      tttcttaac gtttcttcg agtcaaatg tacaaaaac 
      cagaagaag cttctgtat ccggtcaat gtgggtcga 
      aacgttgca cgcgaaacg gcccaaact cactacatt 
      cttccatca gacatcgaa ctctatccc agtcctaat 
      atcattccg caatttctt gcaatgatt gccgaaaat 
      cgcggtcct ttgctcagt aaaaatccc aaagtgttt 
      cctcttcat ttgttcgaa gtgagtgcc aatcagcaa 
      gtacccgaa agcaagaca aagttgatt gaaatgttg 
      aataatggt actgccgta tcgtttcat aaaaaactt 
      tgtcccggt tgtcataat ataccaaaa gcgaaagag 
      tggcaaatg gcgaaagaa accgatgac ctgaaggtg 
      tttcacgtt gggaaacga aacggaagg tatatacat 
      tgggagccg atctttata ggcacgcat gccgatcct 
      ttatacgac gaaaggttg agttgggag ggaaaaagc 
      gataagatg actcaggga tacgctctg tgcgtgttg 
      gattacgac tttctcatc ctcaacaac gcctttctc 
      gtccaccga ccgggcata aaagtctac aaaaaagac 
      caaaagcgt gcaatgttg gccgcaaaa accaatcaa 
      ctcatcaaa aagataatt tttccagaa ctgaaagtg 
      ttgtatggg gtgaaaaaa ggctgtgct gtgtaatta 
      gtggtgtaa atgaacgaa cgacgcttc aaaaagcca 
      taagaaata ttatctagt gtgtatcga tgtactcaa 
      acagtggac ggatttttt gttattaat tgttgcttt 
      gaaattatc gaaatttta tttttttgt gtttttttt 
      ttcgttagg ttagtctca tttattatt gttccgact 
      gatggcaag tctcaagtc tgactattt atttctcag 
      ataggtttt aattccttg tacgtgtgt attatacat 
      aagtaacta ttttttttt tgctcattg tgtacttaa 
      tttttaagt aatttattg taatttttt gccgtttgt 
      acggttcat gtgccttat cgagttgat gtggtgatc 
      ataaaacat attactttt gtactatca caagcgtga 
      atactctca gtatatgcg aataaatat caggaaaaa 
      at
    • >TC011128 RB
      ttgcagaaa ccacgcctt ttcgaattt ttcggatgt 
      ttcgttgac tttccacga atggacaag gtgttagct 
      caagcctct tctccggcc ctttgacca tctggcatg 
      tccctccat tcttacctc cggcatcga cctccaggc 
      cgcaaaaag taaaatgta cagcaaccg caaagcttt 
      cgccgttaa atgcacact gggcatcat cgccatcta 
      cttcttcgt ccatgtgtt gaccaccag tagggccac 
      ggaggccat ccgcgagtt gcaacagtc ggggcgtaa 
      tctcgcagc cctcgagcc catggaccc gcttgatga 
      agccaccaa ggcaccttc cactacacc tcagagcgt 
      cgtcaacgt gtttgaaag ggtgaagga gatcaccag 
      gtgtctgga ccggcctat ggcccccag ggtgcagca 
      aaggggcga ctattgggt gctctacaa ctacgtcat 
      ggctgaaaa aaagtttaa atgtcaaga gagtgtgac 
      gtatacgac gcatgccga ttattcctt catggacaa 
      tctgttgcc tttgctgga caggtggag ggggcccgt 
      gagtctggc cctgcatgc gccagggac ggattttaa 
      aaacacgtt agagtccat cgcctattt gagggactg 
      tacggaggg gctggtccg ggagttggt cacgtttca 
      tgtgtattt tagcacgaa acatgtacc gaaagaagt 
      tcctcgcca caaccacgt cttcaccga gctttacaa 
      ctgctccct tcctccacc ttttcttaa cgtttcttc 
      gagtcaaat gtacaaaaa ccagaagaa gcttctgta 
      tccggtcaa tgtgggtcg aaacgttgc acgcgaaac 
      ggcccaaac tcactacat tcttccatc agacatcga 
      actctatcc cagtcctaa tatcattcc gcaatttct 
      tgcaatgat tgccgaaaa tcgcggtcc tttgctcag 
      taaaaatcc caaagtgtt tcctcttca tttgttcga 
      agtgagtgc caatcagca agtacccga aagcaagac 
      aaagttgat tgaaatgtt gaataatgg tactgccgt 
      atcgtttca taaaaaact ttgtcccgg ttgtcataa 
      tataccaaa agcgaaaga gtggcaaat ggcgaaaga 
      aaccgatga cctgaaggt gtttcacgt tgggaaacg 
      aaacggaag gtatataca ttgggagcc gatctttat 
      aggcacgca tgccgatcc tttatacga cgaaaggtt 
      gagttggga gggaaaaag cgataagat gactcaggg 
      atacgctct gtgcgtgtt ggattacga ctttctcat 
      cctcaacaa cgcctttct cgtccaccg accgggcat 
      aaaagtcta caaaaaaga ccaaaagcg tgcaatgtt 
      ggccgcaaa aaccaatca actcatcaa aaagataat 
      ttttccaga actgaaagt gttgtatgg ggtgaaaaa 
      aggctgtgc tgtgtaatt agtggtgta aatgaacga 
      acgacgctt caaaaagcc ataagaaat attatctag 
      tgtgtatcg atgtactca aacagtgga cggattttt 
      tgttattaa ttgttgctt tgaaattat cgaaatttt 
      atttttttg tgttttttt tttcgttag gttagtctc 
      atttattat tgttccgac tgatggcaa gtctcaagt 
      ctgactatt tatttctca gataggttt taattcctt 
      gtacgtgtg tattataca taagtaact atttttttt 
      ttgctcatt gtgtactta atttttaag taatttatt 
      gtaattttt tgccgtttg tacggttca tgtgcctta 
      tcgagttga tgtggtgat cataaaaca tattacttt 
      tgtactatc acaagcgtg aatactctc agtatatgc 
      gaataaata tcaggaaaa aat