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Gene information
General information
TC011128 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4231854..4251777
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011128
    iBeetle screen iB_01809

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011128 RA
      MSKKVKNAI KINQPLLRP FDHLACPSI LTSGIDLQA 
AKSKMYSNR KAFAVKCTL GIIAIYFFV HVLTTSRAT 
EAIRELQQS GRNLAALEP MDPLDEATK APSTTPQSV 
VNVFERVKE ITRCLDRPM APRVQQRGD YWVLYNYVM 
AEKKFKCQE SVTYTTHAD YSFMDNLLP LLDRWRGPV 
SLALHAPGT DFKNTLESI AYLRDCTEG LVRELVTFH 
VYFSTKHVP KEVPRHNHV FTELYNCSL PPPFLNVSS 
SQMYKNQKK LLYPVNVGR NVARETAQT HYILPSDIE 
LYPSPNIIP QFLAMIAEN RGPLLSKNP KVFPLHLFE 
VSANQQVPE SKTKLIEML NNGTAVSFH KKLCPGCHN 
IPKAKEWQM AKETDDLKV FHVGKRNGR YIHWEPIFI 
GTHADPLYD ERLSWEGKS DKMTQGYAL CVLDYDFLI 
LNNAFLVHR PGIKVYKKD QKRAMLAAK TNQLIKKII 
FPELKVLYG VKKGCAV
    • >TC011128 RB
      MYSNRKAFA VKCTLGIIA IYFFVHVLT TSRATEAIR 
ELQQSGRNL AALEPMDPL DEATKAPST TPQSVVNVF 
ERVKEITRC LDRPMAPRV QQRGDYWVL YNYVMAEKK 
FKCQESVTY TTHADYSFM DNLLPLLDR WRGPVSLAL 
HAPGTDFKN TLESIAYLR DCTEGLVRE LVTFHVYFS 
TKHVPKEVP RHNHVFTEL YNCSLPPPF LNVSSSQMY 
KNQKKLLYP VNVGRNVAR ETAQTHYIL PSDIELYPS 
PNIIPQFLA MIAENRGPL LSKNPKVFP LHLFEVSAN 
QQVPESKTK LIEMLNNGT AVSFHKKLC PGCHNIPKA 
KEWQMAKET DDLKVFHVG KRNGRYIHW EPIFIGTHA 
DPLYDERLS WEGKSDKMT QGYALCVLD YDFLILNNA 
FLVHRPGIK VYKKDQKRA MLAAKTNQL IKKIIFPEL 
KVLYGVKKG CAV
    CDS sequence
    • >TC011128 RA
      atgtcgaaa aaagtgaaa aacgcaatc aaaatcaat 
caacctctt ctccggccc tttgaccat ctggcatgt 
ccctccatt cttacctcc ggcatcgac ctccaggcc 
gcaaaaagt aaaatgtac agcaaccgc aaagctttc 
gccgttaaa tgcacactg ggcatcatc gccatctac 
ttcttcgtc catgtgttg accaccagt agggccacg 
gaggccatc cgcgagttg caacagtcg gggcgtaat 
ctcgcagcc ctcgagccc atggacccg cttgatgaa 
gccaccaag gcaccttcc actacacct cagagcgtc 
gtcaacgtg tttgaaagg gtgaaggag atcaccagg 
tgtctggac cggcctatg gcccccagg gtgcagcaa 
aggggcgac tattgggtg ctctacaac tacgtcatg 
gctgaaaaa aagtttaaa tgtcaagag agtgtgacg 
tatacgacg catgccgat tattccttc atggacaat 
ctgttgcct ttgctggac aggtggagg gggcccgtg 
agtctggcc ctgcatgcg ccagggacg gattttaaa 
aacacgtta gagtccatc gcctatttg agggactgt 
acggagggg ctggtccgg gagttggtc acgtttcat 
gtgtatttt agcacgaaa catgtaccg aaagaagtt 
cctcgccac aaccacgtc ttcaccgag ctttacaac 
tgctccctt cctccacct tttcttaac gtttcttcg 
agtcaaatg tacaaaaac cagaagaag cttctgtat 
ccggtcaat gtgggtcga aacgttgca cgcgaaacg 
gcccaaact cactacatt cttccatca gacatcgaa 
ctctatccc agtcctaat atcattccg caatttctt 
gcaatgatt gccgaaaat cgcggtcct ttgctcagt 
aaaaatccc aaagtgttt cctcttcat ttgttcgaa 
gtgagtgcc aatcagcaa gtacccgaa agcaagaca 
aagttgatt gaaatgttg aataatggt actgccgta 
tcgtttcat aaaaaactt tgtcccggt tgtcataat 
ataccaaaa gcgaaagag tggcaaatg gcgaaagaa 
accgatgac ctgaaggtg tttcacgtt gggaaacga 
aacggaagg tatatacat tgggagccg atctttata 
ggcacgcat gccgatcct ttatacgac gaaaggttg 
agttgggag ggaaaaagc gataagatg actcaggga 
tacgctctg tgcgtgttg gattacgac tttctcatc 
ctcaacaac gcctttctc gtccaccga ccgggcata 
aaagtctac aaaaaagac caaaagcgt gcaatgttg 
gccgcaaaa accaatcaa ctcatcaaa aagataatt 
tttccagaa ctgaaagtg ttgtatggg gtgaaaaaa 
ggctgtgct gtgtaa
    • >TC011128 RB
      atgtacagc aaccgcaaa gctttcgcc gttaaatgc 
acactgggc atcatcgcc atctacttc ttcgtccat 
gtgttgacc accagtagg gccacggag gccatccgc 
gagttgcaa cagtcgggg cgtaatctc gcagccctc 
gagcccatg gacccgctt gatgaagcc accaaggca 
ccttccact acacctcag agcgtcgtc aacgtgttt 
gaaagggtg aaggagatc accaggtgt ctggaccgg 
cctatggcc cccagggtg cagcaaagg ggcgactat 
tgggtgctc tacaactac gtcatggct gaaaaaaag 
tttaaatgt caagagagt gtgacgtat acgacgcat 
gccgattat tccttcatg gacaatctg ttgcctttg 
ctggacagg tggaggggg cccgtgagt ctggccctg 
catgcgcca gggacggat tttaaaaac acgttagag 
tccatcgcc tatttgagg gactgtacg gaggggctg 
gtccgggag ttggtcacg tttcatgtg tattttagc 
acgaaacat gtaccgaaa gaagttcct cgccacaac 
cacgtcttc accgagctt tacaactgc tcccttcct 
ccacctttt cttaacgtt tcttcgagt caaatgtac 
aaaaaccag aagaagctt ctgtatccg gtcaatgtg 
ggtcgaaac gttgcacgc gaaacggcc caaactcac 
tacattctt ccatcagac atcgaactc tatcccagt 
cctaatatc attccgcaa tttcttgca atgattgcc 
gaaaatcgc ggtcctttg ctcagtaaa aatcccaaa 
gtgtttcct cttcatttg ttcgaagtg agtgccaat 
cagcaagta cccgaaagc aagacaaag ttgattgaa 
atgttgaat aatggtact gccgtatcg tttcataaa 
aaactttgt cccggttgt cataatata ccaaaagcg 
aaagagtgg caaatggcg aaagaaacc gatgacctg 
aaggtgttt cacgttggg aaacgaaac ggaaggtat 
atacattgg gagccgatc tttataggc acgcatgcc 
gatccttta tacgacgaa aggttgagt tgggaggga 
aaaagcgat aagatgact cagggatac gctctgtgc 
gtgttggat tacgacttt ctcatcctc aacaacgcc 
tttctcgtc caccgaccg ggcataaaa gtctacaaa 
aaagaccaa aagcgtgca atgttggcc gcaaaaacc 
aatcaactc atcaaaaag ataattttt ccagaactg 
aaagtgttg tatggggtg aaaaaaggc tgtgctgtg 
taa
    mRNA sequence
    • >TC011128 RA
      gactgctgg tgagtggtt cagtctcca gaagcatct 
tgtgtcggt tacagcagt cgaaaacca gcaaaaaat 
tgaacgtcg tttctcaca atgtcgaaa aaagtgaaa 
aacgcaatc aaaatcaat caacctctt ctccggccc 
tttgaccat ctggcatgt ccctccatt cttacctcc 
ggcatcgac ctccaggcc gcaaaaagt aaaatgtac 
agcaaccgc aaagctttc gccgttaaa tgcacactg 
ggcatcatc gccatctac ttcttcgtc catgtgttg 
accaccagt agggccacg gaggccatc cgcgagttg 
caacagtcg gggcgtaat ctcgcagcc ctcgagccc 
atggacccg cttgatgaa gccaccaag gcaccttcc 
actacacct cagagcgtc gtcaacgtg tttgaaagg 
gtgaaggag atcaccagg tgtctggac cggcctatg 
gcccccagg gtgcagcaa aggggcgac tattgggtg 
ctctacaac tacgtcatg gctgaaaaa aagtttaaa 
tgtcaagag agtgtgacg tatacgacg catgccgat 
tattccttc atggacaat ctgttgcct ttgctggac 
aggtggagg gggcccgtg agtctggcc ctgcatgcg 
ccagggacg gattttaaa aacacgtta gagtccatc 
gcctatttg agggactgt acggagggg ctggtccgg 
gagttggtc acgtttcat gtgtatttt agcacgaaa 
catgtaccg aaagaagtt cctcgccac aaccacgtc 
ttcaccgag ctttacaac tgctccctt cctccacct 
tttcttaac gtttcttcg agtcaaatg tacaaaaac 
cagaagaag cttctgtat ccggtcaat gtgggtcga 
aacgttgca cgcgaaacg gcccaaact cactacatt 
cttccatca gacatcgaa ctctatccc agtcctaat 
atcattccg caatttctt gcaatgatt gccgaaaat 
cgcggtcct ttgctcagt aaaaatccc aaagtgttt 
cctcttcat ttgttcgaa gtgagtgcc aatcagcaa 
gtacccgaa agcaagaca aagttgatt gaaatgttg 
aataatggt actgccgta tcgtttcat aaaaaactt 
tgtcccggt tgtcataat ataccaaaa gcgaaagag 
tggcaaatg gcgaaagaa accgatgac ctgaaggtg 
tttcacgtt gggaaacga aacggaagg tatatacat 
tgggagccg atctttata ggcacgcat gccgatcct 
ttatacgac gaaaggttg agttgggag ggaaaaagc 
gataagatg actcaggga tacgctctg tgcgtgttg 
gattacgac tttctcatc ctcaacaac gcctttctc 
gtccaccga ccgggcata aaagtctac aaaaaagac 
caaaagcgt gcaatgttg gccgcaaaa accaatcaa 
ctcatcaaa aagataatt tttccagaa ctgaaagtg 
ttgtatggg gtgaaaaaa ggctgtgct gtgtaatta 
gtggtgtaa atgaacgaa cgacgcttc aaaaagcca 
taagaaata ttatctagt gtgtatcga tgtactcaa 
acagtggac ggatttttt gttattaat tgttgcttt 
gaaattatc gaaatttta tttttttgt gtttttttt 
ttcgttagg ttagtctca tttattatt gttccgact 
gatggcaag tctcaagtc tgactattt atttctcag 
ataggtttt aattccttg tacgtgtgt attatacat 
aagtaacta ttttttttt tgctcattg tgtacttaa 
tttttaagt aatttattg taatttttt gccgtttgt 
acggttcat gtgccttat cgagttgat gtggtgatc 
ataaaacat attactttt gtactatca caagcgtga 
atactctca gtatatgcg aataaatat caggaaaaa 
at
    • >TC011128 RB
      ttgcagaaa ccacgcctt ttcgaattt ttcggatgt 
ttcgttgac tttccacga atggacaag gtgttagct 
caagcctct tctccggcc ctttgacca tctggcatg 
tccctccat tcttacctc cggcatcga cctccaggc 
cgcaaaaag taaaatgta cagcaaccg caaagcttt 
cgccgttaa atgcacact gggcatcat cgccatcta 
cttcttcgt ccatgtgtt gaccaccag tagggccac 
ggaggccat ccgcgagtt gcaacagtc ggggcgtaa 
tctcgcagc cctcgagcc catggaccc gcttgatga 
agccaccaa ggcaccttc cactacacc tcagagcgt 
cgtcaacgt gtttgaaag ggtgaagga gatcaccag 
gtgtctgga ccggcctat ggcccccag ggtgcagca 
aaggggcga ctattgggt gctctacaa ctacgtcat 
ggctgaaaa aaagtttaa atgtcaaga gagtgtgac 
gtatacgac gcatgccga ttattcctt catggacaa 
tctgttgcc tttgctgga caggtggag ggggcccgt 
gagtctggc cctgcatgc gccagggac ggattttaa 
aaacacgtt agagtccat cgcctattt gagggactg 
tacggaggg gctggtccg ggagttggt cacgtttca 
tgtgtattt tagcacgaa acatgtacc gaaagaagt 
tcctcgcca caaccacgt cttcaccga gctttacaa 
ctgctccct tcctccacc ttttcttaa cgtttcttc 
gagtcaaat gtacaaaaa ccagaagaa gcttctgta 
tccggtcaa tgtgggtcg aaacgttgc acgcgaaac 
ggcccaaac tcactacat tcttccatc agacatcga 
actctatcc cagtcctaa tatcattcc gcaatttct 
tgcaatgat tgccgaaaa tcgcggtcc tttgctcag 
taaaaatcc caaagtgtt tcctcttca tttgttcga 
agtgagtgc caatcagca agtacccga aagcaagac 
aaagttgat tgaaatgtt gaataatgg tactgccgt 
atcgtttca taaaaaact ttgtcccgg ttgtcataa 
tataccaaa agcgaaaga gtggcaaat ggcgaaaga 
aaccgatga cctgaaggt gtttcacgt tgggaaacg 
aaacggaag gtatataca ttgggagcc gatctttat 
aggcacgca tgccgatcc tttatacga cgaaaggtt 
gagttggga gggaaaaag cgataagat gactcaggg 
atacgctct gtgcgtgtt ggattacga ctttctcat 
cctcaacaa cgcctttct cgtccaccg accgggcat 
aaaagtcta caaaaaaga ccaaaagcg tgcaatgtt 
ggccgcaaa aaccaatca actcatcaa aaagataat 
ttttccaga actgaaagt gttgtatgg ggtgaaaaa 
aggctgtgc tgtgtaatt agtggtgta aatgaacga 
acgacgctt caaaaagcc ataagaaat attatctag 
tgtgtatcg atgtactca aacagtgga cggattttt 
tgttattaa ttgttgctt tgaaattat cgaaatttt 
atttttttg tgttttttt tttcgttag gttagtctc 
atttattat tgttccgac tgatggcaa gtctcaagt 
ctgactatt tatttctca gataggttt taattcctt 
gtacgtgtg tattataca taagtaact atttttttt 
ttgctcatt gtgtactta atttttaag taatttatt 
gtaattttt tgccgtttg tacggttca tgtgcctta 
tcgagttga tgtggtgat cataaaaca tattacttt 
tgtactatc acaagcgtg aatactctc agtatatgc 
gaataaata tcaggaaaa aat
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