Status
Please wait ...
Gene information
General information
TC011125 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4278155..4280707
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011125
      iBeetle screen iB_01808

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011125 RA
        MYSGSDSTE SQTYPQWAL NPSTYQNMS NEQVDWAAL 
        AQQWIIMKE AGPPPVISS EKAPKNEGG EAPMDVEDK 
        DESLEWHTQ NEWSWNAQN QPWGWTPPM GVPPPVPLK 
        PPLLPTPAN FNYNAPPES VNDNANFTG YSTVPQNDY 
        SSGYWTSAG SSKQVKPHN RRFSKVNVP RPVQPLISE 
        TQPTLDATK RKQLPAWIR EGLEKMERD KLKQLEREK 
        EKQEREEYN EKMKQSEKE TMEILKSTI KEQQKSKFE 
        SDNESEDDD KKAVEPVLT PAEQMMNVR RTMTEILLK 
        VTNHEIENI CREELQRYH KKHKASDRR VSAPSGANV 
        TTKLGLGIY GGSGSSSSD NSGDDKSPE RKDSDTELK 
        DSIKRKKAE FSKTEREIE DKLAVAAER KQSPVTRSP 
        TPEKTNDNH NTQVDAKSQ GRKQTTKKR VVRSSSSSS 
        SRSRSTTPE KSRRKKRYS RRSRSRSRS SSYRRRRSR 
        SRSRSHRRS RRSRSRSGG RGKRSRRR
      CDS sequence
      • >TC011125 RA
        atgtactcg ggtagtgat tcgaccgaa tctcagacc 
        taccctcaa tgggcactc aacccatca acgtaccaa 
        aacatgtcc aacgagcaa gtggattgg gccgctttg 
        gcccaacag tggatcata atgaaggaa gcggggcca 
        ccaccagtt atttcgagc gaaaaggcc ccaaaaaat 
        gagggtggc gaagccccc atggacgtc gaagacaag 
        gacgaatcg cttgaatgg cacacgcag aatgagtgg 
        agttggaat gcgcagaac cagccgtgg ggctggacg 
        ccccccatg ggggtgcca cccccggtg cccctaaag 
        ccccctctt ttgcccacg cccgccaac tttaactat 
        aatgcgccc cctgagagc gtcaacgac aacgccaat 
        ttcactggc tactctact gtaccccaa aacgactat 
        tcgtcgggt tattggact tctgccgga agttcaaaa 
        caagtaaag ccacacaat cgacgattt agtaaagtt 
        aacgttcca cgaccggta caaccccta atcagtgaa 
        acacagcca actttggat gccacgaaa cgtaaacaa 
        ttgccggct tggatacgt gaaggattg gagaaaatg 
        gaacgtgac aaattgaaa caattggag cgcgagaag 
        gagaaacaa gagagagag gaatataac gaaaaaatg 
        aaacaaagt gagaaggaa acaatggag attttaaaa 
        tcgacgatt aaagaacaa caaaagtct aaatttgag 
        agtgataac gaatcggag gacgatgat aagaaggct 
        gtggaacca gtgctgaca cctgccgaa caaatgatg 
        aatgtgagg aggaccatg actgaaata cttctcaaa 
        gtgacaaat catgaaatt gaaaatatc tgtcgcgag 
        gaattgcaa cgttaccac aaaaaacac aaagcttca 
        gatcgtcgc gtttctgca cccagcggt gccaacgta 
        acgacgaaa cttggtttg ggcatttac ggcggctcc 
        ggaagtagc agtagcgac aacagtggc gacgacaaa 
        tccccagaa cgtaaagat tccgatacg gaactaaag 
        gacagtatt aagcgcaaa aaagccgaa ttcagtaaa 
        accgaacgc gaaatcgag gacaagttg gccgtggct 
        gctgaaaga aaacaatca ccggtcaca cgttccccc 
        actcctgaa aaaacaaac gataatcac aacacacag 
        gtagacgca aaatctcaa ggacggaaa caaacgacg 
        aaaaaacgt gttgttcgt tcatcttcg tcgagttcg 
        tcgcgaagt cggtcgaca acacccgaa aaaagccga 
        cgtaaaaaa cgctactct cgaagaagt aggtcgagg 
        tcaaggtcg tcttcgtat cggaggcgg aggtcgagg 
        tccaggtcc aggtcccac cggcgcagc cgacggtca 
        aggtcacgg agtggtggt aggggtaag aggtcgcgc 
        cggcgctga
      mRNA sequence
      • >TC011125 RA
        ggcttgaaa gcccaaatt taatttttc gcgttgcaa 
        tgtagtatt ttttggttg gtcgtgttg gtgtttagt 
        tttgacaat ctggtggga aatgtagcc ggttttcgc 
        tcattttcc atagaattt ggcccaaaa ctagtgaca 
        atcgcagtg aaaattgtt gatttcgtg tcgttagtg 
        atgtaggaa tgtactcgg gtagtgatt cgaccgaat 
        ctcagacct accctcaat gggcactca acccatcaa 
        cgtaccaaa acatgtcca acgagcaag tggattggg 
        ccgctttgg cccaacagt ggatcataa tgaaggaag 
        cggggccac caccagtta tttcgagcg aaaaggccc 
        caaaaaatg agggtggcg aagccccca tggacgtcg 
        aagacaagg acgaatcgc ttgaatggc acacgcaga 
        atgagtgga gttggaatg cgcagaacc agccgtggg 
        gctggacgc cccccatgg gggtgccac ccccggtgc 
        ccctaaagc cccctcttt tgcccacgc ccgccaact 
        ttaactata atgcgcccc ctgagagcg tcaacgaca 
        acgccaatt tcactggct actctactg taccccaaa 
        acgactatt cgtcgggtt attggactt ctgccggaa 
        gttcaaaac aagtaaagc cacacaatc gacgattta 
        gtaaagtta acgttccac gaccggtac aacccctaa 
        tcagtgaaa cacagccaa ctttggatg ccacgaaac 
        gtaaacaat tgccggctt ggatacgtg aaggattgg 
        agaaaatgg aacgtgaca aattgaaac aattggagc 
        gcgagaagg agaaacaag agagagagg aatataacg 
        aaaaaatga aacaaagtg agaaggaaa caatggaga 
        ttttaaaat cgacgatta aagaacaac aaaagtcta 
        aatttgaga gtgataacg aatcggagg acgatgata 
        agaaggctg tggaaccag tgctgacac ctgccgaac 
        aaatgatga atgtgagga ggaccatga ctgaaatac 
        ttctcaaag tgacaaatc atgaaattg aaaatatct 
        gtcgcgagg aattgcaac gttaccaca aaaaacaca 
        aagcttcag atcgtcgcg tttctgcac ccagcggtg 
        ccaacgtaa cgacgaaac ttggtttgg gcatttacg 
        gcggctccg gaagtagca gtagcgaca acagtggcg 
        acgacaaat ccccagaac gtaaagatt ccgatacgg 
        aactaaagg acagtatta agcgcaaaa aagccgaat 
        tcagtaaaa ccgaacgcg aaatcgagg acaagttgg 
        ccgtggctg ctgaaagaa aacaatcac cggtcacac 
        gttccccca ctcctgaaa aaacaaacg ataatcaca 
        acacacagg tagacgcaa aatctcaag gacggaaac 
        aaacgacga aaaaacgtg ttgttcgtt catcttcgt 
        cgagttcgt cgcgaagtc ggtcgacaa cacccgaaa 
        aaagccgac gtaaaaaac gctactctc gaagaagta 
        ggtcgaggt caaggtcgt cttcgtatc ggaggcgga 
        ggtcgaggt ccaggtcca ggtcccacc ggcgcagcc 
        gacggtcaa ggtcacgga gtggtggta ggggtaaga 
        ggtcgcgcc ggcgctgaa gtgtttcga ttttgtttt 
        gttttatgt atataagtg tcttcctag caataatta 
        ttaaagtgt ttagttgag g
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY