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Gene information
General information
TC011120 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 18318..22215
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011120
    iBeetle screen iB_01807

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011120 RA
      MNEVVKLKE KKKNGPGQT HAIEWRVLV VDQLAMRMV 
      SACCKMHDI SAEGITLVE DIHKKREPL ASMEAVYLI 
      TPSEKSVHS LMADFEGPR PMYRGAHVF FTEVCPEEL 
      FNELCKSCA AKKIKTLKE INIAFLPYE SQVFSLDSP 
      DTFQCFYDP SFAAARNAN MERMAEQIA TLCATLGEY 
      PSVRYRSDW ERNVELAQL IQQKLDAYK ADEPTMGEG 
      PEKARSQLL ILDRGFDCF SPLLHELTF QAMAYDLLP 
      IENDVYKYE ASQGVVKEV LLDENDELW VELRHQHIA 
      VVSQSVTKN LKKFTDSKR MTQSDKQSM KDLSQMIKK 
      MPQYQKELS KYATHLHLA EDCMKAYQG YVDKLCKVE 
      QDLAMGTDA EGEKIKDHM RNIVPILLD PKITNEYDK 
      MRIIALYAM TKNGITEEN LSKLATHAQ IKDKQTIAN 
      LQLLGVNVI SDGGNRKKP YTVPRKERI TEQTYQMSR 
      WTPIIKDIM EDCIEDKLD HKHFPFLAG RAQASAYHA 
      PTSARYGHW HKDKTQQTV KNVPRLLVF IVGGMCFSE 
      MRCAYEVTN AVKNWEVIL GSSHILTPE GFMSDLATL 
      TG
    CDS sequence
    • >TC011120 RA
      atgaacgaa gtggtgaaa ctaaaagag aaaaagaag 
      aacggtcca ggacaaacg catgcaatc gaatggagg 
      gtgcttgtg gtggatcag ttggctatg aggatggtg 
      tcggcttgt tgcaaaatg catgatatt tcagctgag 
      gggataaca ctcgtcgaa gatattcac aagaaacgc 
      gaaccgttg gcgtcaatg gaagccgtt tatttgata 
      acaccgtca gaaaagtcg gttcattca cttatggcg 
      gattttgag ggaccccgt ccgatgtac aggggggct 
      catgttttt ttcaccgaa gtctgtccc gaagaactg 
      ttcaatgaa ctgtgtaag tcttgcgct gctaagaaa 
      attaaaaca ctcaaagaa atcaacatt gccttcttg 
      ccgtatgag tcacaggta ttttcattg gattctcct 
      gacactttt caatgtttt tacgatccg agctttgcc 
      gccgctcga aatgccaat atggaacga atggctgaa 
      caaattgca acgttgtgt gcaactctt ggcgaatat 
      ccatccgtt agatatcgc agtgattgg gagcgcaat 
      gtcgagctg gcacagctc atccagcaa aagttggac 
      gcttacaaa gccgacgag ccgacaatg ggcgaaggc 
      cctgaaaaa gcccgatcc caactttta atcctcgat 
      cgtggtttc gattgtttt tcgccgctt ctgcacgaa 
      ttaacattt caagcgatg gcttacgac ctgcttccg 
      atcgaaaac gacgtctac aaatatgag gcgtcacaa 
      ggtgtggta aaagaagtc ctattggac gaaaacgac 
      gaattgtgg gtggagcta cgccaccaa cacatcgca 
      gtcgtctcg caaagcgtc accaaaaac ctgaagaag 
      ttcaccgat tcgaaacgt atgacacag agtgataaa 
      caatcgatg aaagatttg tcccaaatg attaaaaaa 
      atgccacaa taccaaaaa gaactgtca aaatatgcc 
      acacactta catttggcc gaagattgt atgaaagct 
      taccaaggc tatgttgat aaattatgc aaagttgag 
      caagatcta gcgatggga accgacgcc gaaggcgaa 
      aaaatcaaa gatcacatg cgaaacatt gttccaatc 
      ctactcgat ccgaaaatt acaaatgaa tacgataaa 
      atgcgcatt atcgcacta tacgcaatg acaaaaaat 
      ggaatcact gaagaaaac ttatccaag ttggccacg 
      cacgcccaa atcaaggac aaacaaact attgccaat 
      ttacaactc ttgggtgtg aacgtcatc agtgatggt 
      ggtaaccgt aaaaaaccg tacacggtg ccgcgtaaa 
      gaacgcata actgaacag acgtatcaa atgtcaaga 
      tggacgccc attatcaag gacataatg gaggattgc 
      attgaggat aaactcgat cataagcat tttccgttt 
      ttggcaggt cgtgcacag gctagtgct tatcatgca 
      cccaccagt gcccgctat ggccattgg cacaaagac 
      aaaacccag caaactgtg aaaaatgta ccacgcctg 
      ctagttttc atcgtaggc ggtatgtgt ttctctgaa 
      atgaggtgt gcatatgaa gtgacaaat gcagtaaaa 
      aattgggag gttatactc ggatcctca catattctc 
      acacctgaa ggttttatg agcgatttg gcaacgttg 
      actggctag
    mRNA sequence
    • >TC011120 RA
      agtgaatca ccaagtgtc atttaatta tcaaataaa 
      tcgcccaaa aaaacacct cctatcagt ttgtcgtgc 
      tttaatcac caccacaag acgtaaaaa tggccttga 
      aagcccaag ttggattaa aaataatga acgaagtgg 
      tgaaactaa aagagaaaa agaagaacg gtccaggac 
      aaacgcatg caatcgaat ggagggtgc ttgtggtgg 
      atcagttgg ctatgagga tggtgtcgg cttgttgca 
      aaatgcatg atatttcag ctgagggga taacactcg 
      tcgaagata ttcacaaga aacgcgaac cgttggcgt 
      caatggaag ccgtttatt tgataacac cgtcagaaa 
      agtcggttc attcactta tggcggatt ttgagggac 
      cccgtccga tgtacaggg gggctcatg tttttttca 
      ccgaagtct gtcccgaag aactgttca atgaactgt 
      gtaagtctt gcgctgcta agaaaatta aaacactca 
      aagaaatca acattgcct tcttgccgt atgagtcac 
      aggtatttt cattggatt ctcctgaca cttttcaat 
      gtttttacg atccgagct ttgccgccg ctcgaaatg 
      ccaatatgg aacgaatgg ctgaacaaa ttgcaacgt 
      tgtgtgcaa ctcttggcg aatatccat ccgttagat 
      atcgcagtg attgggagc gcaatgtcg agctggcac 
      agctcatcc agcaaaagt tggacgctt acaaagccg 
      acgagccga caatgggcg aaggccctg aaaaagccc 
      gatcccaac ttttaatcc tcgatcgtg gtttcgatt 
      gtttttcgc cgcttctgc acgaattaa catttcaag 
      cgatggctt acgacctgc ttccgatcg aaaacgacg 
      tctacaaat atgaggcgt cacaaggtg tggtaaaag 
      aagtcctat tggacgaaa acgacgaat tgtgggtgg 
      agctacgcc accaacaca tcgcagtcg tctcgcaaa 
      gcgtcacca aaaacctga agaagttca ccgattcga 
      aacgtatga cacagagtg ataaacaat cgatgaaag 
      atttgtccc aaatgatta aaaaaatgc cacaatacc 
      aaaaagaac tgtcaaaat atgccacac acttacatt 
      tggccgaag attgtatga aagcttacc aaggctatg 
      ttgataaat tatgcaaag ttgagcaag atctagcga 
      tgggaaccg acgccgaag gcgaaaaaa tcaaagatc 
      acatgcgaa acattgttc caatcctac tcgatccga 
      aaattacaa atgaatacg ataaaatgc gcattatcg 
      cactatacg caatgacaa aaaatggaa tcactgaag 
      aaaacttat ccaagttgg ccacgcacg cccaaatca 
      aggacaaac aaactattg ccaatttac aactcttgg 
      gtgtgaacg tcatcagtg atggtggta accgtaaaa 
      aaccgtaca cggtgccgc gtaaagaac gcataactg 
      aacagacgt atcaaatgt caagatgga cgcccatta 
      tcaaggaca taatggagg attgcattg aggataaac 
      tcgatcata agcattttc cgtttttgg caggtcgtg 
      cacaggcta gtgcttatc atgcaccca ccagtgccc 
      gctatggcc attggcaca aagacaaaa cccagcaaa 
      ctgtgaaaa atgtaccac gcctgctag ttttcatcg 
      taggcggta tgtgtttct ctgaaatga ggtgtgcat 
      atgaagtga caaatgcag taaaaaatt gggaggtta 
      tactcggat cctcacata ttctcacac ctgaaggtt 
      ttatgagcg atttggcaa cgttgactg gctagaatt 
      caaagagag tcactttta acatacccc ccgatatat 
      gatatatcg cttattatg tcatgtggt gttttgaat 
      gttattgtg ttttttccg tttagatct agggatttt 
      cgtgcttgt taagtttga atgtgttat cgcactaag 
      agattttaa catgttttt tctctccat tctatgcat 
      gatctcgaa caaatggga cgattgatg ttttaggtt 
      agaaattta ggatttgga aataaaggc tgtacttta 
      aaaaaaaac aaacggctt aagtcattt ttctcgctt 
      ccagattgg ataccaaat aagcaatac gtttttttc 
      aaatcaggt tatcgtagg ttagagcat tgttaataa 
      aaaatgata tattaaaaa attagtgac aatattatt 
      gtttttctt ttattattc ggagccgat ttattgtaa 
      ttacactaa aaaatctaa tgtcaattt aaataaata 
      tagcaattt gat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY