Status
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Gene information
General information
TC031386
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011118,TC011117
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 25852..36745
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031386
    iBeetle screen iB_01806

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031386 RA
      MEVFPLIGP NDDFKELWE ADLDPQTMQ EVLRLNAAG 
      DVEWSYMEK DLPGVIIHD RLMTDAALG TRPIKTEHS 
      YSLASDGDS LPDSPISHN KLEEMDDEC FSLPLKKDT 
      ITIKDEPLS ESDSSSCPS SPQSSFMNT SELHFDVEM 
      KPTSQSLLK PSSNVIISS PHNVIPQRI MPKLNIKIE 
      PGHGFHLPP TPPSCSSSE DSEDNATIS QPPSPNVRK 
      TNTRVIMSH ATRQPIHTP LISSQPKGS TGTLVLTDE 
      EKRTLIAEG YPVPTRLPL TKAEEKSLK KIRRKIKNK 
      ISAQESRRK KKEYMDQLE RKVENLVSE NNEYRSKIE 
      SLEDSNTNL LNQLQKLQA IVARTHPQL IKQIK
    CDS sequence
    • >TC031386 RA
      atggaagtg tttccgcta attgggcca aacgacgat 
      ttcaaagaa ttgtgggag gctgacttg gacccgcag 
      acgatgcaa gaagtgctc cgtttgaac gcagcaggc 
      gatgtggaa tggagttat atggaaaag gatttacct 
      ggggttata atacacgat cgactcatg acagatgca 
      gcccttggt acaagaccc ataaaaacc gaacattcg 
      tatagttta gcatcggat ggggattca ctgcccgac 
      tcgcccatt tcccataat aaactagaa gaaatggac 
      gacgagtgc ttttccctg cctttgaaa aaagacacg 
      ataacgata aaagacgaa ccgttgagc gagtccgac 
      tcctcctca tgcccctcc tccccgcag tccagcttc 
      atgaacacg agcgaattg catttcgac gttgaaatg 
      aaacccacc agccaatca ctactcaaa ccctcctca 
      aacgtcatc atcagttcg ccacataac gtcatacct 
      cagcgtatt atgccaaaa ttgaacatt aaaatcgaa 
      ccgggacac ggcttccac ctgcccccg actccgcca 
      tcgtgcagc agcagcgaa gacagcgaa gacaacgcc 
      accatatcg caacccccc tcccccaac gtgcgcaaa 
      accaatacg agagtgatc atgagtcac gcgacccga 
      caaccgata cacacaccc ctgatcagc agccaaccg 
      aaaggatca acggggacc ctagttttg acagacgag 
      gagaaacgg acgctcatt gccgagggg taccccgtc 
      cccaccagg ctgccgctc accaaagct gaggaaaag 
      tcactaaag aaaattcga aggaaaatc aaaaataag 
      atttcggcg caagagagc cgccgaaaa aagaaagag 
      tacatggac cagctcgag cgcaaggtc gaaaatttg 
      gtgtcggag aacaacgaa tatcgcagc aaaatcgag 
      agtcttgag gattcaaac acgaactta ttgaatcag 
      ctccaaaag ttgcaagca atcgtcgcg cgaacacac 
      ccacagttg atcaaacag atcaagtag
    mRNA sequence
    • >TC031386 RA
      aaagtttgc tttagttat gaattccaa tcatttttt 
      aggggtttt ttacaaaat gaaagcaca accttataa 
      tttaaaaaa tttaaggca ccatttaca aacataaca 
      taattttcg ttttatcca aaactgaaa cagttttcg 
      ttgcaaatt ccttgcata ttttcgtcc cttgcccgg 
      aaacacccc aaaaattcc gccctttct ctcccatca 
      tcaaaagtc attaaacct aaccgattc cgaaaagtc 
      agcaattag ccagaacag aaaacgact tggtaataa 
      tgcgcagga cgacaccaa acctcactc actaccgct 
      cacttccac cacacattc aaactcctt tcagatctc 
      gtttccgtc gcgtgtttc atcgccgat ttttcggta 
      aaaacaccg gctagaacc cacccccgg acccaaacg 
      aacccaccc gaaaaaaag gggcgtgtt agccccacg 
      tgtgtcatg accatatgg gaattggtg cagtgagtg 
      ttgaaataa tcgaataaa aaatcaatc aagcgaaaa 
      aaatggaag tgtttccgc taattgggc caaacgacg 
      atttcaaag aattgtggg aggctgact tggacccgc 
      agacgatgc aagaagtgc tccgtttga acgcagcag 
      gcgatgtgg aatggagtt atatggaaa aggatttac 
      ctggggtta taatacacg atcgactca tgacagatg 
      cagcccttg gtacaagac ccataaaaa ccgaacatt 
      cgtatagtt tagcatcgg atggggatt cactgcccg 
      actcgccca tttcccata ataaactag aagaaatgg 
      acgacgagt gcttttccc tgcctttga aaaaagaca 
      cgataacga taaaagacg aaccgttga gcgagtccg 
      actcctcct catgcccct cctccccgc agtccagct 
      tcatgaaca cgagcgaat tgcatttcg acgttgaaa 
      tgaaaccca ccagccaat cactactca aaccctcct 
      caaacgtca tcatcagtt cgccacata acgtcatac 
      ctcagcgta ttatgccaa aattgaaca ttaaaatcg 
      aaccgggac acggcttcc acctgcccc cgactccgc 
      catcgtgca gcagcagcg aagacagcg aagacaacg 
      ccaccatat cgcaacccc cctccccca acgtgcgca 
      aaaccaata cgagagtga tcatgagtc acgcgaccc 
      gacaaccga tacacacac ccctgatca gcagccaac 
      cgaaaggat caacgggga ccctagttt tgacagacg 
      aggagaaac ggacgctca ttgccgagg ggtaccccg 
      tccccacca ggctgccgc tcaccaaag ctgaggaaa 
      agtcactaa agaaaattc gaaggaaaa tcaaaaata 
      agatttcgg cgcaagaga gccgccgaa aaaagaaag 
      agtacatgg accagctcg agcgcaagg tcgaaaatt 
      tggtgtcgg agaacaacg aatatcgca gcaaaatcg 
      agagtcttg aggattcaa acacgaact tattgaatc 
      agctccaaa agttgcaag caatcgtcg cgcgaacac 
      acccacagt tgatcaaac agatcaagt aggctccga 
      gtacaaaaa aaagtcttc catcaacat tccacaaac 
      caaagttta ttatagtgc ggctcaaat atttttgta 
      cttaattag ctgtatata aagcgtgtg ccttaaatt 
      tttctattg ggaccattg tatttattc gactgattc 
      gatccaaga tggcgtgca atgtacata tactatatg 
      tttacgaag tgttgcatt ttatcagag tttctattt 
      tatatatga atgttacta gagtttagc gaatttttt 
      taaatgatg ggtttagat ataatataa cgtttcctc 
      gtctgtttt aggtatgag tgtcacgtc aatatgttc 
      caagtgttg attaattaa ttaatttaa ttaattttt 
      attatattt attattgtc ttaaaggca ggtgcctta 
      ttcatgcag tttctatgg catgagagg tttttaaca 
      cattgtttt ttgtgtgca attttactc cgagttttg 
      gggttttta cttcttttt tgtcatttt atagtgatg 
      atcgtcttg cttttatcg cactgtggg taccaatag 
      tgtctatta ttgtaacgt tttgattga aataaataa 
      caaagcaaa cg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY