General information | |||
TC031386
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450233.1: 25852..36745 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC031386 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01806 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
- >TC031386 RA
MEVFPLIGP NDDFKELWE ADLDPQTMQ EVLRLNAAG DVEWSYMEK DLPGVIIHD RLMTDAALG TRPIKTEHS YSLASDGDS LPDSPISHN KLEEMDDEC FSLPLKKDT ITIKDEPLS ESDSSSCPS SPQSSFMNT SELHFDVEM KPTSQSLLK PSSNVIISS PHNVIPQRI MPKLNIKIE PGHGFHLPP TPPSCSSSE DSEDNATIS QPPSPNVRK TNTRVIMSH ATRQPIHTP LISSQPKGS TGTLVLTDE EKRTLIAEG YPVPTRLPL TKAEEKSLK KIRRKIKNK ISAQESRRK KKEYMDQLE RKVENLVSE NNEYRSKIE SLEDSNTNL LNQLQKLQA IVARTHPQL IKQIK
- >TC031386 RA
atggaagtg tttccgcta attgggcca aacgacgat ttcaaagaa ttgtgggag gctgacttg gacccgcag acgatgcaa gaagtgctc cgtttgaac gcagcaggc gatgtggaa tggagttat atggaaaag gatttacct ggggttata atacacgat cgactcatg acagatgca gcccttggt acaagaccc ataaaaacc gaacattcg tatagttta gcatcggat ggggattca ctgcccgac tcgcccatt tcccataat aaactagaa gaaatggac gacgagtgc ttttccctg cctttgaaa aaagacacg ataacgata aaagacgaa ccgttgagc gagtccgac tcctcctca tgcccctcc tccccgcag tccagcttc atgaacacg agcgaattg catttcgac gttgaaatg aaacccacc agccaatca ctactcaaa ccctcctca aacgtcatc atcagttcg ccacataac gtcatacct cagcgtatt atgccaaaa ttgaacatt aaaatcgaa ccgggacac ggcttccac ctgcccccg actccgcca tcgtgcagc agcagcgaa gacagcgaa gacaacgcc accatatcg caacccccc tcccccaac gtgcgcaaa accaatacg agagtgatc atgagtcac gcgacccga caaccgata cacacaccc ctgatcagc agccaaccg aaaggatca acggggacc ctagttttg acagacgag gagaaacgg acgctcatt gccgagggg taccccgtc cccaccagg ctgccgctc accaaagct gaggaaaag tcactaaag aaaattcga aggaaaatc aaaaataag atttcggcg caagagagc cgccgaaaa aagaaagag tacatggac cagctcgag cgcaaggtc gaaaatttg gtgtcggag aacaacgaa tatcgcagc aaaatcgag agtcttgag gattcaaac acgaactta ttgaatcag ctccaaaag ttgcaagca atcgtcgcg cgaacacac ccacagttg atcaaacag atcaagtag
- >TC031386 RA
aaagtttgc tttagttat gaattccaa tcatttttt aggggtttt ttacaaaat gaaagcaca accttataa tttaaaaaa tttaaggca ccatttaca aacataaca taattttcg ttttatcca aaactgaaa cagttttcg ttgcaaatt ccttgcata ttttcgtcc cttgcccgg aaacacccc aaaaattcc gccctttct ctcccatca tcaaaagtc attaaacct aaccgattc cgaaaagtc agcaattag ccagaacag aaaacgact tggtaataa tgcgcagga cgacaccaa acctcactc actaccgct cacttccac cacacattc aaactcctt tcagatctc gtttccgtc gcgtgtttc atcgccgat ttttcggta aaaacaccg gctagaacc cacccccgg acccaaacg aacccaccc gaaaaaaag gggcgtgtt agccccacg tgtgtcatg accatatgg gaattggtg cagtgagtg ttgaaataa tcgaataaa aaatcaatc aagcgaaaa aaatggaag tgtttccgc taattgggc caaacgacg atttcaaag aattgtggg aggctgact tggacccgc agacgatgc aagaagtgc tccgtttga acgcagcag gcgatgtgg aatggagtt atatggaaa aggatttac ctggggtta taatacacg atcgactca tgacagatg cagcccttg gtacaagac ccataaaaa ccgaacatt cgtatagtt tagcatcgg atggggatt cactgcccg actcgccca tttcccata ataaactag aagaaatgg acgacgagt gcttttccc tgcctttga aaaaagaca cgataacga taaaagacg aaccgttga gcgagtccg actcctcct catgcccct cctccccgc agtccagct tcatgaaca cgagcgaat tgcatttcg acgttgaaa tgaaaccca ccagccaat cactactca aaccctcct caaacgtca tcatcagtt cgccacata acgtcatac ctcagcgta ttatgccaa aattgaaca ttaaaatcg aaccgggac acggcttcc acctgcccc cgactccgc catcgtgca gcagcagcg aagacagcg aagacaacg ccaccatat cgcaacccc cctccccca acgtgcgca aaaccaata cgagagtga tcatgagtc acgcgaccc gacaaccga tacacacac ccctgatca gcagccaac cgaaaggat caacgggga ccctagttt tgacagacg aggagaaac ggacgctca ttgccgagg ggtaccccg tccccacca ggctgccgc tcaccaaag ctgaggaaa agtcactaa agaaaattc gaaggaaaa tcaaaaata agatttcgg cgcaagaga gccgccgaa aaaagaaag agtacatgg accagctcg agcgcaagg tcgaaaatt tggtgtcgg agaacaacg aatatcgca gcaaaatcg agagtcttg aggattcaa acacgaact tattgaatc agctccaaa agttgcaag caatcgtcg cgcgaacac acccacagt tgatcaaac agatcaagt aggctccga gtacaaaaa aaagtcttc catcaacat tccacaaac caaagttta ttatagtgc ggctcaaat atttttgta cttaattag ctgtatata aagcgtgtg ccttaaatt tttctattg ggaccattg tatttattc gactgattc gatccaaga tggcgtgca atgtacata tactatatg tttacgaag tgttgcatt ttatcagag tttctattt tatatatga atgttacta gagtttagc gaatttttt taaatgatg ggtttagat ataatataa cgtttcctc gtctgtttt aggtatgag tgtcacgtc aatatgttc caagtgttg attaattaa ttaatttaa ttaattttt attatattt attattgtc ttaaaggca ggtgcctta ttcatgcag tttctatgg catgagagg tttttaaca cattgtttt ttgtgtgca attttactc cgagttttg gggttttta cttcttttt tgtcatttt atagtgatg atcgtcttg cttttatcg cactgtggg taccaatag tgtctatta ttgtaacgt tttgattga aataaataa caaagcaaa cg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
