Status
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Gene information
General information
TC011114 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 68540..83374
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011114
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01805

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Pupal morphology
    remarks: later than schedule,
    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 2

    wings bent - Number of animals showing the phenotype: 2

    oblique dorsal thoracic muscles thinner - Number of animals showing the phenotype: 2

    oblique dorsal thoracic muscles decreased - Number of animals showing the phenotype: 2

    longitudinal dorsal thoracic muscles sometimes thinner - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011114 RA
      MFGCYDSYS YRLWSLANV WNTCQLSPL TNGVSNKES 
DDLNTTTSS NGDYYIKCP QCQKGCQTF HALKEHMET 
SHADLTASS PENGLLTTT TTTTSTSPN VAVSPTTPS 
TGGPFGCSQ CTTSFATKD QLDKHELLH SPNAQVSCK 
ICNKTFANV YRLQRHMIS HDESAVLRK FKCTECDKA 
FKFKHHLKE HIRIHSGEK PFECPNCGK RFSHSGSYS 
SHMTSKKCL VMNLKLGRA RPNTPQNHR PLKQQRPIN 
NNINTSPNH NTYLPILPK YSEFLQPPF YLAPPNLPS 
ISPYSIPSL GHIFEQLQQ SPLRPPLPP GDDYVQTPP 
IKEEELKSN TSSCGELVM DEDETTKNE ETAPNNTGD 
LEAVKRILE TVNATVTKQ LLQANISKF SSESSSSDN 
SSQHSPKEE QHETSEGLA AKLEDIVQV KEEDEEIDS 
ESVTTTDHV SEDGRKVRV RSLISDEQL KVLKDHYKL 
NPRPKREDL EKIADTIGF PVRVVQVWF QNTRARDRR 
EGRLIQVPY SPALRYPLV PSSLTISPY NEQPLDLST 
KRGPPSIES STPGSSPRP ELDHEAINL TKTPPSPID 
FNNSSRLAQ ILAQPKLSM GTMGLVPME QWDLPSLSQ 
LITNRLNSL SPKIDDGGD EKRGKVSQY VLKSLGSPV 
LGGGGDGEV EGQFSCDQC DKAFSKQSS LARHKYEHS 
GQRPHKCDE CPKAFKHKH HLTEHKRLH SGEKPFQCV 
KCLKRFSHS GSYSQHMNH RYSYCKPYR E
    • >TC011114 RB
      MVARSESMF GCYDSYSYR LWSLANVWN TCQLSPLTN 
GVSNKESDD LNTTTSSNG DYYIKCPQC QKGCQTFHA 
LKEHMETSH ADLTASSPE NGLLTTTTT TTSTSPNVA 
VSPTTPSTG GPFGCSQCT TSFATKDQL DKHELLHSP 
NAQVSCKIC NKTFANVYR LQRHMISHD ESAVLRKFK 
CTECDKAFK FKHHLKEHI RIHSGEKPF ECPNCGKRF 
SHSGSYSSH MTSKKCLVM NLKLGRARP NTPQNHRPL 
KQQRPINNN INTSPNHNT YLPILPKYS EFLQPPFYL 
APPNLPSIS PYSIPSLGH IFEQLQQSP LRPPLPPGD 
DYVQTPPIK EEELKSNTS SCGELVMDE DETTKNEET 
APNNTGDLE AVKRILETV NATVTKQLL QANISKFSS 
ESSSSDNSS QHSPKEEQH ETSEGLAAK LEDIVQVKE 
EDEEIDSES VTTTDHVSE DGRKVRVRS LISDEQLKV 
LKDHYKLNP RPKREDLEK IADTIGFPV RVVQVWFQN 
TRARDRREG RLIQVPYSP ALRYPLVPS SLTISPYNE 
QPLDLSTKR GPPSIESST PGSSPRPEL DHEAINLTK 
TPPSPIDFN NSSRLAQIL AQPKLSMGT MGLVPMEQW 
DLPSLSQLI TNRLNSLSP KIDDGGDEK RGKVSQYVL 
KSLGSPVLG GGGDGEVEG QFSCDQCDK AFSKQSSLA 
RHKYEHSGQ RPHKCDECP KAFKHKHHL TEHKRLHSG 
EKPFQCVKC LKRFSHSGS YSQHMNHRY SYCKPYRE
    CDS sequence
    • >TC011114 RA
      atgtttggt tgttacgac agttactcc tatcgttta 
tggagtcta gctaatgta tggaacacc tgccaactt 
tccccgttg acaaacggt gtgagtaat aaggaaagc 
gacgatctc aacaccacc accagctca aacggcgac 
tattacatc aaatgtccg cagtgtcag aaaggatgt 
cagacgttt catgcgttg aaagagcac atggagacg 
tcgcacgcc gatttgaca gcttcaagt cctgaaaat 
ggactttta acaacgacg acgacaacg acgtccaca 
tcaccaaac gtcgccgtc tcgccgacg acgccttcc 
actggaggt cccttcggg tgctcgcaa tgcaccacc 
tcctttgcc accaaagac caactggac aaacacgaa 
ttgttgcat tcgcctaat gctcaagtg tcctgtaaa 
atctgcaac aaaaccttc gccaacgtg taccgcctt 
caacgtcac atgatcagc catgacgag agcgccgta 
cttcgcaaa ttcaaatgt accgaatgc gataaggcc 
tttaaattc aaacaccat ttaaaggaa cacattcga 
atccacagt ggcgaaaaa ccattcgaa tgtcccaac 
tgtggcaaa cgcttctcc cactcagga tcctattct 
agtcacatg acatcgaaa aaatgcctc gtgatgaat 
ttaaaattg ggtcgtgca cgtcccaac accccccaa 
aatcatcgc cccttaaaa caacaacgt ccaatcaac 
aataacatc aacacaagt cccaaccac aacacctat 
cttccaatt cttccaaaa tattccgaa tttcttcaa 
ccacccttc tatctggca ccgccaaac ctcccatca 
atcagtcca tactctatt ccgagttta ggccacatt 
tttgaacag ttacaacaa tcaccactc cgacctccc 
ctccctcct ggcgatgat tacgttcaa acgccacca 
atcaaagaa gaagaactc aagtcgaac acgtccagt 
tgtggcgaa ctggtgatg gacgaagac gaaacaacg 
aagaatgaa gaaaccgcc cccaataat accggcgac 
ttggaggcg gtaaaacgc atcctggaa acagtcaac 
gcaactgta accaaacag ttgttacag gccaacatt 
tcaaaattc tcctcggaa tcatcatca agtgacaac 
tcatcacaa cattccccc aaagaagaa caacacgaa 
acaagcgaa ggtctcgcc gcaaaactg gaagatatc 
gtccaagtg aaagaagaa gatgaagaa atcgacagc 
gaatcggtt accaccacc gatcacgtg tcagaagac 
ggccgtaaa gtccgagtc cgttcgtta atttccgac 
gaacaattg aaagtactc aaggatcac tacaagcta 
aatccgcgc ccaaaacgc gaagatttg gagaaaatc 
gccgacacg attggtttt ccagttcgt gtggtccag 
gtgtggttt cagaacaca agggcccgt gaccgccgc 
gaagggcgc ctcatacag gtgccctac agtccggcc 
ctgcgctac cctttggtg ccttcctca ctcactatc 
tcaccctac aatgaacaa ccgctagat ttgtcaacg 
aaacgagga ccaccgtca atcgaatcc tcaactccc 
ggtagttcc cccaggcct gaactggac catgaagct 
attaattta actaaaaca ccaccatcg cccattgat 
tttaataat agtagtcgg ttggcacaa attctagca 
caaccgaaa ttatcaatg ggtacgatg ggtttagtg 
ccaatggaa caatgggat ttacccagt ttaagccaa 
ttgattacg aatcgtttg aattcgtta agtccgaaa 
attgacgat ggaggggat gagaagagg gggaaagtg 
tcacagtat gtgttgaaa agtctaggg agtcctgta 
cttggaggt ggaggagat ggagaagtg gaggggcag 
tttagttgt gatcaatgt gataaggcg ttttcgaag 
cagagctca ttggcgagg cacaaatat gagcattca 
ggtcaaagg ccgcacaaa tgcgacgag tgtcccaag 
gccttcaag cacaagcac cacctgacc gagcacaag 
cgtctccac agcggcgag aagccgttt cagtgcgtg 
aaatgtctg aaacgtttt tcacactct ggttcctac 
agccagcat atgaaccat cgctattcg tattgtaag 
ccgtacaga gagtag
    • >TC011114 RB
      atggtggcg cgcagtgaa agcatgttt ggttgttac 
gacagttac tcctatcgt ttatggagt ctagctaat 
gtatggaac acctgccaa ctttccccg ttgacaaac 
ggtgtgagt aataaggaa agcgacgat ctcaacacc 
accaccagc tcaaacggc gactattac atcaaatgt 
ccgcagtgt cagaaagga tgtcagacg tttcatgcg 
ttgaaagag cacatggag acgtcgcac gccgatttg 
acagcttca agtcctgaa aatggactt ttaacaacg 
acgacgaca acgacgtcc acatcacca aacgtcgcc 
gtctcgccg acgacgcct tccactgga ggtcccttc 
gggtgctcg caatgcacc acctccttt gccaccaaa 
gaccaactg gacaaacac gaattgttg cattcgcct 
aatgctcaa gtgtcctgt aaaatctgc aacaaaacc 
ttcgccaac gtgtaccgc cttcaacgt cacatgatc 
agccatgac gagagcgcc gtacttcgc aaattcaaa 
tgtaccgaa tgcgataag gcctttaaa ttcaaacac 
catttaaag gaacacatt cgaatccac agtggcgaa 
aaaccattc gaatgtccc aactgtggc aaacgcttc 
tcccactca ggatcctat tctagtcac atgacatcg 
aaaaaatgc ctcgtgatg aatttaaaa ttgggtcgt 
gcacgtccc aacaccccc caaaatcat cgcccctta 
aaacaacaa cgtccaatc aacaataac atcaacaca 
agtcccaac cacaacacc tatcttcca attcttcca 
aaatattcc gaatttctt caaccaccc ttctatctg 
gcaccgcca aacctccca tcaatcagt ccatactct 
attccgagt ttaggccac atttttgaa cagttacaa 
caatcacca ctccgacct cccctccct cctggcgat 
gattacgtt caaacgcca ccaatcaaa gaagaagaa 
ctcaagtcg aacacgtcc agttgtggc gaactggtg 
atggacgaa gacgaaaca acgaagaat gaagaaacc 
gcccccaat aataccggc gacttggag gcggtaaaa 
cgcatcctg gaaacagtc aacgcaact gtaaccaaa 
cagttgtta caggccaac atttcaaaa ttctcctcg 
gaatcatca tcaagtgac aactcatca caacattcc 
cccaaagaa gaacaacac gaaacaagc gaaggtctc 
gccgcaaaa ctggaagat atcgtccaa gtgaaagaa 
gaagatgaa gaaatcgac agcgaatcg gttaccacc 
accgatcac gtgtcagaa gacggccgt aaagtccga 
gtccgttcg ttaatttcc gacgaacaa ttgaaagta 
ctcaaggat cactacaag ctaaatccg cgcccaaaa 
cgcgaagat ttggagaaa atcgccgac acgattggt 
tttccagtt cgtgtggtc caggtgtgg tttcagaac 
acaagggcc cgtgaccgc cgcgaaggg cgcctcata 
caggtgccc tacagtccg gccctgcgc taccctttg 
gtgccttcc tcactcact atctcaccc tacaatgaa 
caaccgcta gatttgtca acgaaacga ggaccaccg 
tcaatcgaa tcctcaact cccggtagt tcccccagg 
cctgaactg gaccatgaa gctattaat ttaactaaa 
acaccacca tcgcccatt gattttaat aatagtagt 
cggttggca caaattcta gcacaaccg aaattatca 
atgggtacg atgggttta gtgccaatg gaacaatgg 
gatttaccc agtttaagc caattgatt acgaatcgt 
ttgaattcg ttaagtccg aaaattgac gatggaggg 
gatgagaag agggggaaa gtgtcacag tatgtgttg 
aaaagtcta gggagtcct gtacttgga ggtggagga 
gatggagaa gtggagggg cagtttagt tgtgatcaa 
tgtgataag gcgttttcg aagcagagc tcattggcg 
aggcacaaa tatgagcat tcaggtcaa aggccgcac 
aaatgcgac gagtgtccc aaggccttc aagcacaag 
caccacctg accgagcac aagcgtctc cacagcggc 
gagaagccg tttcagtgc gtgaaatgt ctgaaacgt 
ttttcacac tctggttcc tacagccag catatgaac 
catcgctat tcgtattgt aagccgtac agagagtag
    mRNA sequence
    • >TC011114 RA
      ttgcgtgat tttgtgcgt ttggcgtcg cgacgtcct 
ctctccggc cgctttcgc atcgaaatg ccgcttatc 
gagggcggc gttggtccg gcgtcggcg acgtcgttt 
tgcgcgcgc cggtggtgg gggattcat ctcctgtgt 
ggaggaggg agggatgtt tagtcagtg ttggagcgt 
cgcgtgtcg ctttcggtt cagttggcc atggtggcg 
cgcagtgaa agcatgttt ggttgttac gacagttac 
tcctatcgt ttatggagt ctagctaat gtatggaac 
acctgccaa ctttccccg ttgacaaac ggtgtgagt 
aataaggaa agcgacgat ctcaacacc accaccagc 
tcaaacggc gactattac atcaaatgt ccgcagtgt 
cagaaagga tgtcagacg tttcatgcg ttgaaagag 
cacatggag acgtcgcac gccgatttg acagcttca 
agtcctgaa aatggactt ttaacaacg acgacgaca 
acgacgtcc acatcacca aacgtcgcc gtctcgccg 
acgacgcct tccactgga ggtcccttc gggtgctcg 
caatgcacc acctccttt gccaccaaa gaccaactg 
gacaaacac gaattgttg cattcgcct aatgctcaa 
gtgtcctgt aaaatctgc aacaaaacc ttcgccaac 
gtgtaccgc cttcaacgt cacatgatc agccatgac 
gagagcgcc gtacttcgc aaattcaaa tgtaccgaa 
tgcgataag gcctttaaa ttcaaacac catttaaag 
gaacacatt cgaatccac agtggcgaa aaaccattc 
gaatgtccc aactgtggc aaacgcttc tcccactca 
ggatcctat tctagtcac atgacatcg aaaaaatgc 
ctcgtgatg aatttaaaa ttgggtcgt gcacgtccc 
aacaccccc caaaatcat cgcccctta aaacaacaa 
cgtccaatc aacaataac atcaacaca agtcccaac 
cacaacacc tatcttcca attcttcca aaatattcc 
gaatttctt caaccaccc ttctatctg gcaccgcca 
aacctccca tcaatcagt ccatactct attccgagt 
ttaggccac atttttgaa cagttacaa caatcacca 
ctccgacct cccctccct cctggcgat gattacgtt 
caaacgcca ccaatcaaa gaagaagaa ctcaagtcg 
aacacgtcc agttgtggc gaactggtg atggacgaa 
gacgaaaca acgaagaat gaagaaacc gcccccaat 
aataccggc gacttggag gcggtaaaa cgcatcctg 
gaaacagtc aacgcaact gtaaccaaa cagttgtta 
caggccaac atttcaaaa ttctcctcg gaatcatca 
tcaagtgac aactcatca caacattcc cccaaagaa 
gaacaacac gaaacaagc gaaggtctc gccgcaaaa 
ctggaagat atcgtccaa gtgaaagaa gaagatgaa 
gaaatcgac agcgaatcg gttaccacc accgatcac 
gtgtcagaa gacggccgt aaagtccga gtccgttcg 
ttaatttcc gacgaacaa ttgaaagta ctcaaggat 
cactacaag ctaaatccg cgcccaaaa cgcgaagat 
ttggagaaa atcgccgac acgattggt tttccagtt 
cgtgtggtc caggtgtgg tttcagaac acaagggcc 
cgtgaccgc cgcgaaggg cgcctcata caggtgccc 
tacagtccg gccctgcgc taccctttg gtgccttcc 
tcactcact atctcaccc tacaatgaa caaccgcta 
gatttgtca acgaaacga ggaccaccg tcaatcgaa 
tcctcaact cccggtagt tcccccagg cctgaactg 
gaccatgaa gctattaat ttaactaaa acaccacca 
tcgcccatt gattttaat aatagtagt cggttggca 
caaattcta gcacaaccg aaattatca atgggtacg 
atgggttta gtgccaatg gaacaatgg gatttaccc 
agtttaagc caattgatt acgaatcgt ttgaattcg 
ttaagtccg aaaattgac gatggaggg gatgagaag 
agggggaaa gtgtcacag tatgtgttg aaaagtcta 
gggagtcct gtacttgga ggtggagga gatggagaa 
gtggagggg cagtttagt tgtgatcaa tgtgataag 
gcgttttcg aagcagagc tcattggcg aggcacaaa 
tatgagcat tcaggtcaa aggccgcac aaatgcgac 
gagtgtccc aaggccttc aagcacaag caccacctg 
accgagcac aagcgtctc cacagcggc gagaagccg 
tttcagtgc gtgaaatgt ctgaaacgt ttttcacac 
tctggttcc tacagccag catatgaac catcgctat 
tcgtattgt aagccgtac agagagtag attcggttg 
cgaatcagt tccagtact tagactttg accttcttg 
ccacgcaaa aaacccaaa tgttgtaaa tagtattta 
aaagagagt ttataagag tttcgttgg ttttgaaat 
tagcatgta catacttgt ctggtacct gtataacaa 
tactccgat ttaattaac tgattaaag tatacagtt 
tcgtgccag ttgaatcaa atgtcttcc agcaaaggg 
cagctggag gcagagtgt tcgatgtgc aatgcgccg 
tagttacgt tattgtgat tggaatatt taaaatatt 
tttttattg tagatattg taaaataaa acgtgttgc 
tttcgaagt gccatacac accaacgat tgtataata 
gcctttgtg tgattgtag attggtttc ggaacatcg 
atattgata gttatatgc ctttagttg ataacagta 
caataaggg ggcgtttca tgacaatac gtagtaacc 
aaacaatac aatgaacaa atggtgcaa ctaactcac 
ttttataag aagcattnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnnnnn nnnncccag ccgccgcga 
tgaaaccat tccaaattg cgtacctaa gattattat 
ttccagcgt gtgactaat ggataagat ttgttgaca 
ataatgtag ccatttgat ataaaccta tttacttag 
tactgctaa tgttaattc ataaagtag catttatta 
ttaaagaat tctcaaggt atgtgagac attttcgaa 
tattaaaat atgtataca acattatat aatattaaa 
ggaatataa attttaata attacttaa gatattcta 
cgatttcag tgtatagct atttctgta catattcaa 
tgtttactt ctccctctc tctcttcct ttctctagt 
gcatgtccg accgttcgt tcgttcttt cctcaattt 
ttttactat tatcctact cgtaattta gtttattcg 
tttgcaata ttgttgtgt gtaataatt tttatttta 
tttgttaac aaaaaatgt aaatttttt taccaatgc 
catggctag tataaccaa agattttgt aatatatat 
tagttctat gtatatttt tttcttgtt ttttttttt 
ttcatttac atgtccagt ttatgtaac attcttttt 
gttattatt ataattata tttttttac tttctagat 
tgtcttgta aaaatggtg attataaag tatgtctgt 
gtcaaattc tattatgaa aagttggaa attgtattt 
tactcgata caaataaac tataaattg ccat
    • >TC011114 RB
      ttgcgtgat tttgtgcgt ttggcgtcg cgacgtcct 
ctctccggc cgctttcgc atcgaaatg ccgcttatc 
gagggcggc gttggtccg gcgtcggcg acgtcgttt 
tgcgcgcgc cggtggtgg gggattcat ctcctgtgt 
ggaggaggg agggatgtt tagtcagtg ttggagcgt 
cgcgtgtcg ctttcggtt cagttggcc atggtggcg 
cgcagtgaa agcatgttt ggttgttac gacagttac 
tcctatcgt ttatggagt ctagctaat gtatggaac 
acctgccaa ctttccccg ttgacaaac ggtgtgagt 
aataaggaa agcgacgat ctcaacacc accaccagc 
tcaaacggc gactattac atcaaatgt ccgcagtgt 
cagaaagga tgtcagacg tttcatgcg ttgaaagag 
cacatggag acgtcgcac gccgatttg acagcttca 
agtcctgaa aatggactt ttaacaacg acgacgaca 
acgacgtcc acatcacca aacgtcgcc gtctcgccg 
acgacgcct tccactgga ggtcccttc gggtgctcg 
caatgcacc acctccttt gccaccaaa gaccaactg 
gacaaacac gaattgttg cattcgcct aatgctcaa 
gtgtcctgt aaaatctgc aacaaaacc ttcgccaac 
gtgtaccgc cttcaacgt cacatgatc agccatgac 
gagagcgcc gtacttcgc aaattcaaa tgtaccgaa 
tgcgataag gcctttaaa ttcaaacac catttaaag 
gaacacatt cgaatccac agtggcgaa aaaccattc 
gaatgtccc aactgtggc aaacgcttc tcccactca 
ggatcctat tctagtcac atgacatcg aaaaaatgc 
ctcgtgatg aatttaaaa ttgggtcgt gcacgtccc 
aacaccccc caaaatcat cgcccctta aaacaacaa 
cgtccaatc aacaataac atcaacaca agtcccaac 
cacaacacc tatcttcca attcttcca aaatattcc 
gaatttctt caaccaccc ttctatctg gcaccgcca 
aacctccca tcaatcagt ccatactct attccgagt 
ttaggccac atttttgaa cagttacaa caatcacca 
ctccgacct cccctccct cctggcgat gattacgtt 
caaacgcca ccaatcaaa gaagaagaa ctcaagtcg 
aacacgtcc agttgtggc gaactggtg atggacgaa 
gacgaaaca acgaagaat gaagaaacc gcccccaat 
aataccggc gacttggag gcggtaaaa cgcatcctg 
gaaacagtc aacgcaact gtaaccaaa cagttgtta 
caggccaac atttcaaaa ttctcctcg gaatcatca 
tcaagtgac aactcatca caacattcc cccaaagaa 
gaacaacac gaaacaagc gaaggtctc gccgcaaaa 
ctggaagat atcgtccaa gtgaaagaa gaagatgaa 
gaaatcgac agcgaatcg gttaccacc accgatcac 
gtgtcagaa gacggccgt aaagtccga gtccgttcg 
ttaatttcc gacgaacaa ttgaaagta ctcaaggat 
cactacaag ctaaatccg cgcccaaaa cgcgaagat 
ttggagaaa atcgccgac acgattggt tttccagtt 
cgtgtggtc caggtgtgg tttcagaac acaagggcc 
cgtgaccgc cgcgaaggg cgcctcata caggtgccc 
tacagtccg gccctgcgc taccctttg gtgccttcc 
tcactcact atctcaccc tacaatgaa caaccgcta 
gatttgtca acgaaacga ggaccaccg tcaatcgaa 
tcctcaact cccggtagt tcccccagg cctgaactg 
gaccatgaa gctattaat ttaactaaa acaccacca 
tcgcccatt gattttaat aatagtagt cggttggca 
caaattcta gcacaaccg aaattatca atgggtacg 
atgggttta gtgccaatg gaacaatgg gatttaccc 
agtttaagc caattgatt acgaatcgt ttgaattcg 
ttaagtccg aaaattgac gatggaggg gatgagaag 
agggggaaa gtgtcacag tatgtgttg aaaagtcta 
gggagtcct gtacttgga ggtggagga gatggagaa 
gtggagggg cagtttagt tgtgatcaa tgtgataag 
gcgttttcg aagcagagc tcattggcg aggcacaaa 
tatgagcat tcaggtcaa aggccgcac aaatgcgac 
gagtgtccc aaggccttc aagcacaag caccacctg 
accgagcac aagcgtctc cacagcggc gagaagccg 
tttcagtgc gtgaaatgt ctgaaacgt ttttcacac 
tctggttcc tacagccag catatgaac catcgctat 
tcgtattgt aagccgtac agagagtag attcggttg 
cgaatcagt tccagtact tagactttg accttcttg 
ccacgcaaa aaacccaaa tgttgtaaa tagtattta 
aaagagagt ttataagag tttcgttgg ttttgaaat 
tagcatgta catacttgt ctggtacct gtataacaa 
tactccgat ttaattaac tgattaaag tatacagtt 
tcgtgccag ttgaatcaa atgtcttcc agcaaaggg 
cagctggag gcagagtgt tcgatgtgc aatgcgccg 
tagttacgt tattgtgat tggaatatt taaaatatt 
tttttattg tagatattg taaaataaa acgtgttgc 
tttcgaagt gccatacac accaacgat tgtataata 
gcctttgtg tgattgtag attggtttc ggaacatcg 
atattgata gttatatgc ctttagttg ataacagta 
caataaggg ggcgtttca tgacaatac gtagtaacc 
aaacaatac aatgaacaa atggtgcaa ctaactcac 
ttttataag aagcattnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnnnnn nnnncccag ccgccgcga 
tgaaaccat tccaaattg cgtacctaa gattattat 
ttccagcgt gtgactaat ggataagat ttgttgaca 
ataatgtag ccatttgat ataaaccta tttacttag 
tactgctaa tgttaattc ataaagtag catttatta 
ttaaagaat tctcaaggt atgtgagac attttcgaa 
tattaaaat atgtataca acattatat aatattaaa 
ggaatataa attttaata attacttaa gatattcta 
cgatttcag tgtatagct atttctgta catattcaa 
tgtttactt ctccctctc tctcttcct ttctctagt 
gcatgtccg accgttcgt tcgttcttt cctcaattt 
ttttactat tatcctact cgtaattta gtttattcg 
tttgcaata ttgttgtgt gtaataatt tttatttta 
tttgttaac aaaaaatgt aaatttttt taccaatgc 
catggctag tataaccaa agattttgt aatatatat 
tagttctat gtatatttt tttcttgtt ttttttttt 
ttcatttac atgtccagt ttatgtaac attcttttt 
gttattatt ataattata tttttttac tttctagat 
tgtcttgta aaaatggtg attataaag tatgtctgt 
gtcaaattc tattatgaa aagttggaa attgtattt 
tactcgata caaataaac tataaattg ccat
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