Status
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Gene information
General information
TC011114 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 68540..83374
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011114
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01805

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 2

    wings bent - Number of animals showing the phenotype: 2

    oblique dorsal thoracic muscles thinner - Number of animals showing the phenotype: 2

    oblique dorsal thoracic muscles decreased - Number of animals showing the phenotype: 2

    longitudinal dorsal thoracic muscles sometimes thinner - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011114 RA
      MFGCYDSYS YRLWSLANV WNTCQLSPL TNGVSNKES 
      DDLNTTTSS NGDYYIKCP QCQKGCQTF HALKEHMET 
      SHADLTASS PENGLLTTT TTTTSTSPN VAVSPTTPS 
      TGGPFGCSQ CTTSFATKD QLDKHELLH SPNAQVSCK 
      ICNKTFANV YRLQRHMIS HDESAVLRK FKCTECDKA 
      FKFKHHLKE HIRIHSGEK PFECPNCGK RFSHSGSYS 
      SHMTSKKCL VMNLKLGRA RPNTPQNHR PLKQQRPIN 
      NNINTSPNH NTYLPILPK YSEFLQPPF YLAPPNLPS 
      ISPYSIPSL GHIFEQLQQ SPLRPPLPP GDDYVQTPP 
      IKEEELKSN TSSCGELVM DEDETTKNE ETAPNNTGD 
      LEAVKRILE TVNATVTKQ LLQANISKF SSESSSSDN 
      SSQHSPKEE QHETSEGLA AKLEDIVQV KEEDEEIDS 
      ESVTTTDHV SEDGRKVRV RSLISDEQL KVLKDHYKL 
      NPRPKREDL EKIADTIGF PVRVVQVWF QNTRARDRR 
      EGRLIQVPY SPALRYPLV PSSLTISPY NEQPLDLST 
      KRGPPSIES STPGSSPRP ELDHEAINL TKTPPSPID 
      FNNSSRLAQ ILAQPKLSM GTMGLVPME QWDLPSLSQ 
      LITNRLNSL SPKIDDGGD EKRGKVSQY VLKSLGSPV 
      LGGGGDGEV EGQFSCDQC DKAFSKQSS LARHKYEHS 
      GQRPHKCDE CPKAFKHKH HLTEHKRLH SGEKPFQCV 
      KCLKRFSHS GSYSQHMNH RYSYCKPYR E
    • >TC011114 RB
      MVARSESMF GCYDSYSYR LWSLANVWN TCQLSPLTN 
      GVSNKESDD LNTTTSSNG DYYIKCPQC QKGCQTFHA 
      LKEHMETSH ADLTASSPE NGLLTTTTT TTSTSPNVA 
      VSPTTPSTG GPFGCSQCT TSFATKDQL DKHELLHSP 
      NAQVSCKIC NKTFANVYR LQRHMISHD ESAVLRKFK 
      CTECDKAFK FKHHLKEHI RIHSGEKPF ECPNCGKRF 
      SHSGSYSSH MTSKKCLVM NLKLGRARP NTPQNHRPL 
      KQQRPINNN INTSPNHNT YLPILPKYS EFLQPPFYL 
      APPNLPSIS PYSIPSLGH IFEQLQQSP LRPPLPPGD 
      DYVQTPPIK EEELKSNTS SCGELVMDE DETTKNEET 
      APNNTGDLE AVKRILETV NATVTKQLL QANISKFSS 
      ESSSSDNSS QHSPKEEQH ETSEGLAAK LEDIVQVKE 
      EDEEIDSES VTTTDHVSE DGRKVRVRS LISDEQLKV 
      LKDHYKLNP RPKREDLEK IADTIGFPV RVVQVWFQN 
      TRARDRREG RLIQVPYSP ALRYPLVPS SLTISPYNE 
      QPLDLSTKR GPPSIESST PGSSPRPEL DHEAINLTK 
      TPPSPIDFN NSSRLAQIL AQPKLSMGT MGLVPMEQW 
      DLPSLSQLI TNRLNSLSP KIDDGGDEK RGKVSQYVL 
      KSLGSPVLG GGGDGEVEG QFSCDQCDK AFSKQSSLA 
      RHKYEHSGQ RPHKCDECP KAFKHKHHL TEHKRLHSG 
      EKPFQCVKC LKRFSHSGS YSQHMNHRY SYCKPYRE
    CDS sequence
    • >TC011114 RA
      atgtttggt tgttacgac agttactcc tatcgttta 
      tggagtcta gctaatgta tggaacacc tgccaactt 
      tccccgttg acaaacggt gtgagtaat aaggaaagc 
      gacgatctc aacaccacc accagctca aacggcgac 
      tattacatc aaatgtccg cagtgtcag aaaggatgt 
      cagacgttt catgcgttg aaagagcac atggagacg 
      tcgcacgcc gatttgaca gcttcaagt cctgaaaat 
      ggactttta acaacgacg acgacaacg acgtccaca 
      tcaccaaac gtcgccgtc tcgccgacg acgccttcc 
      actggaggt cccttcggg tgctcgcaa tgcaccacc 
      tcctttgcc accaaagac caactggac aaacacgaa 
      ttgttgcat tcgcctaat gctcaagtg tcctgtaaa 
      atctgcaac aaaaccttc gccaacgtg taccgcctt 
      caacgtcac atgatcagc catgacgag agcgccgta 
      cttcgcaaa ttcaaatgt accgaatgc gataaggcc 
      tttaaattc aaacaccat ttaaaggaa cacattcga 
      atccacagt ggcgaaaaa ccattcgaa tgtcccaac 
      tgtggcaaa cgcttctcc cactcagga tcctattct 
      agtcacatg acatcgaaa aaatgcctc gtgatgaat 
      ttaaaattg ggtcgtgca cgtcccaac accccccaa 
      aatcatcgc cccttaaaa caacaacgt ccaatcaac 
      aataacatc aacacaagt cccaaccac aacacctat 
      cttccaatt cttccaaaa tattccgaa tttcttcaa 
      ccacccttc tatctggca ccgccaaac ctcccatca 
      atcagtcca tactctatt ccgagttta ggccacatt 
      tttgaacag ttacaacaa tcaccactc cgacctccc 
      ctccctcct ggcgatgat tacgttcaa acgccacca 
      atcaaagaa gaagaactc aagtcgaac acgtccagt 
      tgtggcgaa ctggtgatg gacgaagac gaaacaacg 
      aagaatgaa gaaaccgcc cccaataat accggcgac 
      ttggaggcg gtaaaacgc atcctggaa acagtcaac 
      gcaactgta accaaacag ttgttacag gccaacatt 
      tcaaaattc tcctcggaa tcatcatca agtgacaac 
      tcatcacaa cattccccc aaagaagaa caacacgaa 
      acaagcgaa ggtctcgcc gcaaaactg gaagatatc 
      gtccaagtg aaagaagaa gatgaagaa atcgacagc 
      gaatcggtt accaccacc gatcacgtg tcagaagac 
      ggccgtaaa gtccgagtc cgttcgtta atttccgac 
      gaacaattg aaagtactc aaggatcac tacaagcta 
      aatccgcgc ccaaaacgc gaagatttg gagaaaatc 
      gccgacacg attggtttt ccagttcgt gtggtccag 
      gtgtggttt cagaacaca agggcccgt gaccgccgc 
      gaagggcgc ctcatacag gtgccctac agtccggcc 
      ctgcgctac cctttggtg ccttcctca ctcactatc 
      tcaccctac aatgaacaa ccgctagat ttgtcaacg 
      aaacgagga ccaccgtca atcgaatcc tcaactccc 
      ggtagttcc cccaggcct gaactggac catgaagct 
      attaattta actaaaaca ccaccatcg cccattgat 
      tttaataat agtagtcgg ttggcacaa attctagca 
      caaccgaaa ttatcaatg ggtacgatg ggtttagtg 
      ccaatggaa caatgggat ttacccagt ttaagccaa 
      ttgattacg aatcgtttg aattcgtta agtccgaaa 
      attgacgat ggaggggat gagaagagg gggaaagtg 
      tcacagtat gtgttgaaa agtctaggg agtcctgta 
      cttggaggt ggaggagat ggagaagtg gaggggcag 
      tttagttgt gatcaatgt gataaggcg ttttcgaag 
      cagagctca ttggcgagg cacaaatat gagcattca 
      ggtcaaagg ccgcacaaa tgcgacgag tgtcccaag 
      gccttcaag cacaagcac cacctgacc gagcacaag 
      cgtctccac agcggcgag aagccgttt cagtgcgtg 
      aaatgtctg aaacgtttt tcacactct ggttcctac 
      agccagcat atgaaccat cgctattcg tattgtaag 
      ccgtacaga gagtag
    • >TC011114 RB
      atggtggcg cgcagtgaa agcatgttt ggttgttac 
      gacagttac tcctatcgt ttatggagt ctagctaat 
      gtatggaac acctgccaa ctttccccg ttgacaaac 
      ggtgtgagt aataaggaa agcgacgat ctcaacacc 
      accaccagc tcaaacggc gactattac atcaaatgt 
      ccgcagtgt cagaaagga tgtcagacg tttcatgcg 
      ttgaaagag cacatggag acgtcgcac gccgatttg 
      acagcttca agtcctgaa aatggactt ttaacaacg 
      acgacgaca acgacgtcc acatcacca aacgtcgcc 
      gtctcgccg acgacgcct tccactgga ggtcccttc 
      gggtgctcg caatgcacc acctccttt gccaccaaa 
      gaccaactg gacaaacac gaattgttg cattcgcct 
      aatgctcaa gtgtcctgt aaaatctgc aacaaaacc 
      ttcgccaac gtgtaccgc cttcaacgt cacatgatc 
      agccatgac gagagcgcc gtacttcgc aaattcaaa 
      tgtaccgaa tgcgataag gcctttaaa ttcaaacac 
      catttaaag gaacacatt cgaatccac agtggcgaa 
      aaaccattc gaatgtccc aactgtggc aaacgcttc 
      tcccactca ggatcctat tctagtcac atgacatcg 
      aaaaaatgc ctcgtgatg aatttaaaa ttgggtcgt 
      gcacgtccc aacaccccc caaaatcat cgcccctta 
      aaacaacaa cgtccaatc aacaataac atcaacaca 
      agtcccaac cacaacacc tatcttcca attcttcca 
      aaatattcc gaatttctt caaccaccc ttctatctg 
      gcaccgcca aacctccca tcaatcagt ccatactct 
      attccgagt ttaggccac atttttgaa cagttacaa 
      caatcacca ctccgacct cccctccct cctggcgat 
      gattacgtt caaacgcca ccaatcaaa gaagaagaa 
      ctcaagtcg aacacgtcc agttgtggc gaactggtg 
      atggacgaa gacgaaaca acgaagaat gaagaaacc 
      gcccccaat aataccggc gacttggag gcggtaaaa 
      cgcatcctg gaaacagtc aacgcaact gtaaccaaa 
      cagttgtta caggccaac atttcaaaa ttctcctcg 
      gaatcatca tcaagtgac aactcatca caacattcc 
      cccaaagaa gaacaacac gaaacaagc gaaggtctc 
      gccgcaaaa ctggaagat atcgtccaa gtgaaagaa 
      gaagatgaa gaaatcgac agcgaatcg gttaccacc 
      accgatcac gtgtcagaa gacggccgt aaagtccga 
      gtccgttcg ttaatttcc gacgaacaa ttgaaagta 
      ctcaaggat cactacaag ctaaatccg cgcccaaaa 
      cgcgaagat ttggagaaa atcgccgac acgattggt 
      tttccagtt cgtgtggtc caggtgtgg tttcagaac 
      acaagggcc cgtgaccgc cgcgaaggg cgcctcata 
      caggtgccc tacagtccg gccctgcgc taccctttg 
      gtgccttcc tcactcact atctcaccc tacaatgaa 
      caaccgcta gatttgtca acgaaacga ggaccaccg 
      tcaatcgaa tcctcaact cccggtagt tcccccagg 
      cctgaactg gaccatgaa gctattaat ttaactaaa 
      acaccacca tcgcccatt gattttaat aatagtagt 
      cggttggca caaattcta gcacaaccg aaattatca 
      atgggtacg atgggttta gtgccaatg gaacaatgg 
      gatttaccc agtttaagc caattgatt acgaatcgt 
      ttgaattcg ttaagtccg aaaattgac gatggaggg 
      gatgagaag agggggaaa gtgtcacag tatgtgttg 
      aaaagtcta gggagtcct gtacttgga ggtggagga 
      gatggagaa gtggagggg cagtttagt tgtgatcaa 
      tgtgataag gcgttttcg aagcagagc tcattggcg 
      aggcacaaa tatgagcat tcaggtcaa aggccgcac 
      aaatgcgac gagtgtccc aaggccttc aagcacaag 
      caccacctg accgagcac aagcgtctc cacagcggc 
      gagaagccg tttcagtgc gtgaaatgt ctgaaacgt 
      ttttcacac tctggttcc tacagccag catatgaac 
      catcgctat tcgtattgt aagccgtac agagagtag
    mRNA sequence
    • >TC011114 RA
      ttgcgtgat tttgtgcgt ttggcgtcg cgacgtcct 
      ctctccggc cgctttcgc atcgaaatg ccgcttatc 
      gagggcggc gttggtccg gcgtcggcg acgtcgttt 
      tgcgcgcgc cggtggtgg gggattcat ctcctgtgt 
      ggaggaggg agggatgtt tagtcagtg ttggagcgt 
      cgcgtgtcg ctttcggtt cagttggcc atggtggcg 
      cgcagtgaa agcatgttt ggttgttac gacagttac 
      tcctatcgt ttatggagt ctagctaat gtatggaac 
      acctgccaa ctttccccg ttgacaaac ggtgtgagt 
      aataaggaa agcgacgat ctcaacacc accaccagc 
      tcaaacggc gactattac atcaaatgt ccgcagtgt 
      cagaaagga tgtcagacg tttcatgcg ttgaaagag 
      cacatggag acgtcgcac gccgatttg acagcttca 
      agtcctgaa aatggactt ttaacaacg acgacgaca 
      acgacgtcc acatcacca aacgtcgcc gtctcgccg 
      acgacgcct tccactgga ggtcccttc gggtgctcg 
      caatgcacc acctccttt gccaccaaa gaccaactg 
      gacaaacac gaattgttg cattcgcct aatgctcaa 
      gtgtcctgt aaaatctgc aacaaaacc ttcgccaac 
      gtgtaccgc cttcaacgt cacatgatc agccatgac 
      gagagcgcc gtacttcgc aaattcaaa tgtaccgaa 
      tgcgataag gcctttaaa ttcaaacac catttaaag 
      gaacacatt cgaatccac agtggcgaa aaaccattc 
      gaatgtccc aactgtggc aaacgcttc tcccactca 
      ggatcctat tctagtcac atgacatcg aaaaaatgc 
      ctcgtgatg aatttaaaa ttgggtcgt gcacgtccc 
      aacaccccc caaaatcat cgcccctta aaacaacaa 
      cgtccaatc aacaataac atcaacaca agtcccaac 
      cacaacacc tatcttcca attcttcca aaatattcc 
      gaatttctt caaccaccc ttctatctg gcaccgcca 
      aacctccca tcaatcagt ccatactct attccgagt 
      ttaggccac atttttgaa cagttacaa caatcacca 
      ctccgacct cccctccct cctggcgat gattacgtt 
      caaacgcca ccaatcaaa gaagaagaa ctcaagtcg 
      aacacgtcc agttgtggc gaactggtg atggacgaa 
      gacgaaaca acgaagaat gaagaaacc gcccccaat 
      aataccggc gacttggag gcggtaaaa cgcatcctg 
      gaaacagtc aacgcaact gtaaccaaa cagttgtta 
      caggccaac atttcaaaa ttctcctcg gaatcatca 
      tcaagtgac aactcatca caacattcc cccaaagaa 
      gaacaacac gaaacaagc gaaggtctc gccgcaaaa 
      ctggaagat atcgtccaa gtgaaagaa gaagatgaa 
      gaaatcgac agcgaatcg gttaccacc accgatcac 
      gtgtcagaa gacggccgt aaagtccga gtccgttcg 
      ttaatttcc gacgaacaa ttgaaagta ctcaaggat 
      cactacaag ctaaatccg cgcccaaaa cgcgaagat 
      ttggagaaa atcgccgac acgattggt tttccagtt 
      cgtgtggtc caggtgtgg tttcagaac acaagggcc 
      cgtgaccgc cgcgaaggg cgcctcata caggtgccc 
      tacagtccg gccctgcgc taccctttg gtgccttcc 
      tcactcact atctcaccc tacaatgaa caaccgcta 
      gatttgtca acgaaacga ggaccaccg tcaatcgaa 
      tcctcaact cccggtagt tcccccagg cctgaactg 
      gaccatgaa gctattaat ttaactaaa acaccacca 
      tcgcccatt gattttaat aatagtagt cggttggca 
      caaattcta gcacaaccg aaattatca atgggtacg 
      atgggttta gtgccaatg gaacaatgg gatttaccc 
      agtttaagc caattgatt acgaatcgt ttgaattcg 
      ttaagtccg aaaattgac gatggaggg gatgagaag 
      agggggaaa gtgtcacag tatgtgttg aaaagtcta 
      gggagtcct gtacttgga ggtggagga gatggagaa 
      gtggagggg cagtttagt tgtgatcaa tgtgataag 
      gcgttttcg aagcagagc tcattggcg aggcacaaa 
      tatgagcat tcaggtcaa aggccgcac aaatgcgac 
      gagtgtccc aaggccttc aagcacaag caccacctg 
      accgagcac aagcgtctc cacagcggc gagaagccg 
      tttcagtgc gtgaaatgt ctgaaacgt ttttcacac 
      tctggttcc tacagccag catatgaac catcgctat 
      tcgtattgt aagccgtac agagagtag attcggttg 
      cgaatcagt tccagtact tagactttg accttcttg 
      ccacgcaaa aaacccaaa tgttgtaaa tagtattta 
      aaagagagt ttataagag tttcgttgg ttttgaaat 
      tagcatgta catacttgt ctggtacct gtataacaa 
      tactccgat ttaattaac tgattaaag tatacagtt 
      tcgtgccag ttgaatcaa atgtcttcc agcaaaggg 
      cagctggag gcagagtgt tcgatgtgc aatgcgccg 
      tagttacgt tattgtgat tggaatatt taaaatatt 
      tttttattg tagatattg taaaataaa acgtgttgc 
      tttcgaagt gccatacac accaacgat tgtataata 
      gcctttgtg tgattgtag attggtttc ggaacatcg 
      atattgata gttatatgc ctttagttg ataacagta 
      caataaggg ggcgtttca tgacaatac gtagtaacc 
      aaacaatac aatgaacaa atggtgcaa ctaactcac 
      ttttataag aagcattnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnncccag ccgccgcga 
      tgaaaccat tccaaattg cgtacctaa gattattat 
      ttccagcgt gtgactaat ggataagat ttgttgaca 
      ataatgtag ccatttgat ataaaccta tttacttag 
      tactgctaa tgttaattc ataaagtag catttatta 
      ttaaagaat tctcaaggt atgtgagac attttcgaa 
      tattaaaat atgtataca acattatat aatattaaa 
      ggaatataa attttaata attacttaa gatattcta 
      cgatttcag tgtatagct atttctgta catattcaa 
      tgtttactt ctccctctc tctcttcct ttctctagt 
      gcatgtccg accgttcgt tcgttcttt cctcaattt 
      ttttactat tatcctact cgtaattta gtttattcg 
      tttgcaata ttgttgtgt gtaataatt tttatttta 
      tttgttaac aaaaaatgt aaatttttt taccaatgc 
      catggctag tataaccaa agattttgt aatatatat 
      tagttctat gtatatttt tttcttgtt ttttttttt 
      ttcatttac atgtccagt ttatgtaac attcttttt 
      gttattatt ataattata tttttttac tttctagat 
      tgtcttgta aaaatggtg attataaag tatgtctgt 
      gtcaaattc tattatgaa aagttggaa attgtattt 
      tactcgata caaataaac tataaattg ccat
    • >TC011114 RB
      ttgcgtgat tttgtgcgt ttggcgtcg cgacgtcct 
      ctctccggc cgctttcgc atcgaaatg ccgcttatc 
      gagggcggc gttggtccg gcgtcggcg acgtcgttt 
      tgcgcgcgc cggtggtgg gggattcat ctcctgtgt 
      ggaggaggg agggatgtt tagtcagtg ttggagcgt 
      cgcgtgtcg ctttcggtt cagttggcc atggtggcg 
      cgcagtgaa agcatgttt ggttgttac gacagttac 
      tcctatcgt ttatggagt ctagctaat gtatggaac 
      acctgccaa ctttccccg ttgacaaac ggtgtgagt 
      aataaggaa agcgacgat ctcaacacc accaccagc 
      tcaaacggc gactattac atcaaatgt ccgcagtgt 
      cagaaagga tgtcagacg tttcatgcg ttgaaagag 
      cacatggag acgtcgcac gccgatttg acagcttca 
      agtcctgaa aatggactt ttaacaacg acgacgaca 
      acgacgtcc acatcacca aacgtcgcc gtctcgccg 
      acgacgcct tccactgga ggtcccttc gggtgctcg 
      caatgcacc acctccttt gccaccaaa gaccaactg 
      gacaaacac gaattgttg cattcgcct aatgctcaa 
      gtgtcctgt aaaatctgc aacaaaacc ttcgccaac 
      gtgtaccgc cttcaacgt cacatgatc agccatgac 
      gagagcgcc gtacttcgc aaattcaaa tgtaccgaa 
      tgcgataag gcctttaaa ttcaaacac catttaaag 
      gaacacatt cgaatccac agtggcgaa aaaccattc 
      gaatgtccc aactgtggc aaacgcttc tcccactca 
      ggatcctat tctagtcac atgacatcg aaaaaatgc 
      ctcgtgatg aatttaaaa ttgggtcgt gcacgtccc 
      aacaccccc caaaatcat cgcccctta aaacaacaa 
      cgtccaatc aacaataac atcaacaca agtcccaac 
      cacaacacc tatcttcca attcttcca aaatattcc 
      gaatttctt caaccaccc ttctatctg gcaccgcca 
      aacctccca tcaatcagt ccatactct attccgagt 
      ttaggccac atttttgaa cagttacaa caatcacca 
      ctccgacct cccctccct cctggcgat gattacgtt 
      caaacgcca ccaatcaaa gaagaagaa ctcaagtcg 
      aacacgtcc agttgtggc gaactggtg atggacgaa 
      gacgaaaca acgaagaat gaagaaacc gcccccaat 
      aataccggc gacttggag gcggtaaaa cgcatcctg 
      gaaacagtc aacgcaact gtaaccaaa cagttgtta 
      caggccaac atttcaaaa ttctcctcg gaatcatca 
      tcaagtgac aactcatca caacattcc cccaaagaa 
      gaacaacac gaaacaagc gaaggtctc gccgcaaaa 
      ctggaagat atcgtccaa gtgaaagaa gaagatgaa 
      gaaatcgac agcgaatcg gttaccacc accgatcac 
      gtgtcagaa gacggccgt aaagtccga gtccgttcg 
      ttaatttcc gacgaacaa ttgaaagta ctcaaggat 
      cactacaag ctaaatccg cgcccaaaa cgcgaagat 
      ttggagaaa atcgccgac acgattggt tttccagtt 
      cgtgtggtc caggtgtgg tttcagaac acaagggcc 
      cgtgaccgc cgcgaaggg cgcctcata caggtgccc 
      tacagtccg gccctgcgc taccctttg gtgccttcc 
      tcactcact atctcaccc tacaatgaa caaccgcta 
      gatttgtca acgaaacga ggaccaccg tcaatcgaa 
      tcctcaact cccggtagt tcccccagg cctgaactg 
      gaccatgaa gctattaat ttaactaaa acaccacca 
      tcgcccatt gattttaat aatagtagt cggttggca 
      caaattcta gcacaaccg aaattatca atgggtacg 
      atgggttta gtgccaatg gaacaatgg gatttaccc 
      agtttaagc caattgatt acgaatcgt ttgaattcg 
      ttaagtccg aaaattgac gatggaggg gatgagaag 
      agggggaaa gtgtcacag tatgtgttg aaaagtcta 
      gggagtcct gtacttgga ggtggagga gatggagaa 
      gtggagggg cagtttagt tgtgatcaa tgtgataag 
      gcgttttcg aagcagagc tcattggcg aggcacaaa 
      tatgagcat tcaggtcaa aggccgcac aaatgcgac 
      gagtgtccc aaggccttc aagcacaag caccacctg 
      accgagcac aagcgtctc cacagcggc gagaagccg 
      tttcagtgc gtgaaatgt ctgaaacgt ttttcacac 
      tctggttcc tacagccag catatgaac catcgctat 
      tcgtattgt aagccgtac agagagtag attcggttg 
      cgaatcagt tccagtact tagactttg accttcttg 
      ccacgcaaa aaacccaaa tgttgtaaa tagtattta 
      aaagagagt ttataagag tttcgttgg ttttgaaat 
      tagcatgta catacttgt ctggtacct gtataacaa 
      tactccgat ttaattaac tgattaaag tatacagtt 
      tcgtgccag ttgaatcaa atgtcttcc agcaaaggg 
      cagctggag gcagagtgt tcgatgtgc aatgcgccg 
      tagttacgt tattgtgat tggaatatt taaaatatt 
      tttttattg tagatattg taaaataaa acgtgttgc 
      tttcgaagt gccatacac accaacgat tgtataata 
      gcctttgtg tgattgtag attggtttc ggaacatcg 
      atattgata gttatatgc ctttagttg ataacagta 
      caataaggg ggcgtttca tgacaatac gtagtaacc 
      aaacaatac aatgaacaa atggtgcaa ctaactcac 
      ttttataag aagcattnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnncccag ccgccgcga 
      tgaaaccat tccaaattg cgtacctaa gattattat 
      ttccagcgt gtgactaat ggataagat ttgttgaca 
      ataatgtag ccatttgat ataaaccta tttacttag 
      tactgctaa tgttaattc ataaagtag catttatta 
      ttaaagaat tctcaaggt atgtgagac attttcgaa 
      tattaaaat atgtataca acattatat aatattaaa 
      ggaatataa attttaata attacttaa gatattcta 
      cgatttcag tgtatagct atttctgta catattcaa 
      tgtttactt ctccctctc tctcttcct ttctctagt 
      gcatgtccg accgttcgt tcgttcttt cctcaattt 
      ttttactat tatcctact cgtaattta gtttattcg 
      tttgcaata ttgttgtgt gtaataatt tttatttta 
      tttgttaac aaaaaatgt aaatttttt taccaatgc 
      catggctag tataaccaa agattttgt aatatatat 
      tagttctat gtatatttt tttcttgtt ttttttttt 
      ttcatttac atgtccagt ttatgtaac attcttttt 
      gttattatt ataattata tttttttac tttctagat 
      tgtcttgta aaaatggtg attataaag tatgtctgt 
      gtcaaattc tattatgaa aagttggaa attgtattt 
      tactcgata caaataaac tataaattg ccat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY