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Gene information
General information
TC011106 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid105: 262910..265058
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011106
    iBeetle screen iB_01804
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011106 RA
      MSDLSEKIL RHVSETSPL NTLDLAQLL ATDHQKIVG 
      ALKSIQAHG DLIRAEPQT SKFIELTEE GKQVAEHGS 
      HEARIFHAV PSEGVAQAE LMRLPNAKV GFSKAMSAG 
      WLTLDKGGP PVVKRKVDK IVDTVQANL RLIQGGHLG 
      QIEDSVRQE YKKRKLIQE VVQKSFNLT KGGDFSLTL 
      AKLETDLTS EMLLTGGWQ QLKFKDYNF DALGAPLDC 
      GYLHPLLKV RAEFRQIFL EMGFSEMPT NNYVESSFW 
      NFDALFQPQ QHPARDAHD TFFVSDPKI STNFPEDYK 
      ERVKEVHSR GGYGSLGYR YDWKIEEAQ KNLLRTHTT 
      ANSARMLYK LAQEKNFKP TKLFSIDKV FRNETLDAT 
      HLAEFNQVE GVIADYDVT LGDLIGVFA EFFKKLGIT 
      ELEFKPAYN PYTEPSMEI FCFHNGLGK WIEIGNSGM 
      FRPEMLLTM GLPENVRVI AWGLSLERP TMIKYGLNN 
      IRDLVGPKV DLEMVQRSP ICRLDK
    CDS sequence
    • >TC011106 RA
      atgtcagac ctcagtgaa aaaatcctc cgacacgtg 
      tcggagact tcgcccctc aacaccctc gacctcgcc 
      cagctcctt gccaccgac caccagaaa atcgtgggg 
      gctctcaag agcatccaa gcccacggc gacctcatc 
      agggcggag ccccaaacc tccaaattc atcgaactg 
      accgaggag ggcaaacaa gtggcggag cacggcagt 
      cacgaagcc cgcattttc cacgctgtg ccctccgag 
      ggcgtagcc caagccgag cttatgaga ctccccaac 
      gccaaggtc ggcttcagc aaggccatg agcgctggc 
      tggctgacc ttggacaaa gggggcccc cctgtcgtc 
      aagcgaaaa gtcgacaaa atcgtggac acagtccag 
      gcaaattta cgcctgatt caagggggg cacttgggg 
      caaatcgaa gacagcgtg agacaggag tacaagaag 
      cgaaaattg atacaagag gtggtccag aagtctttt 
      aatttgact aaggggggc gactttagc ctgaccttg 
      gccaagttg gagaccgat ttaacgtcc gaaatgttg 
      ctaacaggg ggctggcaa caactgaaa ttcaaagac 
      tataatttt gacgccctg ggggcccct ttggactgc 
      ggctacttg caccccctg ctcaaggtc agggccgaa 
      ttcaggcaa attttcctc gaaatgggg ttcagcgaa 
      atgcccacg aacaattat gttgagagt tctttctgg 
      aattttgac gcacttttc cagccccaa caacaccca 
      gcaagggac gcccatgac acgtttttt gtctcagat 
      ccgaaaatt tcaacgaat tttccagaa gattacaaa 
      gaacgggtc aaggaggtg cacagtagg ggcggctat 
      ggatcttta ggttaccgc tacgattgg aaaatcgaa 
      gaggcgcaa aaaaacctc ctccgcacc cacactaca 
      gccaacagc gcccgcatg ttatacaaa ctagcacag 
      gaaaaaaat ttcaaaccg actaaactc ttcagtatt 
      gataaagtt ttccgcaat gaaacactc gatgccaca 
      catttggcg gaatttaac caagttgag ggcgtcata 
      gccgactat gacgtcacg cttggtgac ttaatcggt 
      gtttttgcc gaatttttc aaaaaactg gggatcaca 
      gagttggag tttaaacca gcttataac ccctacact 
      gagcccagt atggaaatt ttctgtttc cataatggt 
      ttaggaaag tggatcgag atcggtaat tcggggatg 
      ttccgaccc gaaatgttg ctcacaatg gggctgccg 
      gaaaatgtg agggtgatt gcctggggg ctgtctttg 
      gaacggccc actatgatc aaatatggg ttgaataat 
      attagggat ttagttggg ccaaaggtt gatttggag 
      atggtccag aggagtccg atttgtagg ttagataaa 
      taa
    mRNA sequence
    • >TC011106 RA
      ttacgcaac ctttggtaa ctttaactg tcacagttg 
      gcaccatcg tcgcaaatg tgcgtgaag tgtcaaaac 
      cacgtcaaa aaattgcat caccccgac agcccccat 
      aatgtcaga cctcagtga aaaaatcct ccgacacgt 
      gtcggagac ttcgcccct caacaccct cgacctcgc 
      ccagctcct tgccaccga ccaccagaa aatcgtggg 
      ggctctcaa gagcatcca agcccacgg cgacctcat 
      cagggcgga gccccaaac ctccaaatt catcgaact 
      gaccgagga gggcaaaca agtggcgga gcacggcag 
      tcacgaagc ccgcatttt ccacgctgt gccctccga 
      gggcgtagc ccaagccga gcttatgag actccccaa 
      cgccaaggt cggcttcag caaggccat gagcgctgg 
      ctggctgac cttggacaa agggggccc ccctgtcgt 
      caagcgaaa agtcgacaa aatcgtgga cacagtcca 
      ggcaaattt acgcctgat tcaaggggg gcacttggg 
      gcaaatcga agacagcgt gagacagga gtacaagaa 
      gcgaaaatt gatacaaga ggtggtcca gaagtcttt 
      taatttgac taagggggg cgactttag cctgacctt 
      ggccaagtt ggagaccga tttaacgtc cgaaatgtt 
      gctaacagg gggctggca acaactgaa attcaaaga 
      ctataattt tgacgccct gggggcccc tttggactg 
      cggctactt gcaccccct gctcaaggt cagggccga 
      attcaggca aattttcct cgaaatggg gttcagcga 
      aatgcccac gaacaatta tgttgagag ttctttctg 
      gaattttga cgcactttt ccagcccca acaacaccc 
      agcaaggga cgcccatga cacgttttt tgtctcaga 
      tccgaaaat ttcaacgaa ttttccaga agattacaa 
      agaacgggt caaggaggt gcacagtag gggcggcta 
      tggatcttt aggttaccg ctacgattg gaaaatcga 
      agaggcgca aaaaaacct cctccgcac ccacactac 
      agccaacag cgcccgcat gttatacaa actagcaca 
      ggaaaaaaa tttcaaacc gactaaact cttcagtat 
      tgataaagt tttccgcaa tgaaacact cgatgccac 
      acatttggc ggaatttaa ccaagttga gggcgtcat 
      agccgacta tgacgtcac gcttggtga cttaatcgg 
      tgtttttgc cgaattttt caaaaaact ggggatcac 
      agagttgga gtttaaacc agcttataa cccctacac 
      tgagcccag tatggaaat tttctgttt ccataatgg 
      tttaggaaa gtggatcga gatcggtaa ttcggggat 
      gttccgacc cgaaatgtt gctcacaat ggggctgcc 
      ggaaaatgt gagggtgat tgcctgggg gctgtcttt 
      ggaacggcc cactatgat caaatatgg gttgaataa 
      tattaggga tttagttgg gccaaaggt tgatttgga 
      gatggtcca gaggagtcc gatttgtag gttagataa 
      ataagataa tttgtattt tttgttttt ataataaat 
      tatttaagc aaca