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Gene information
General information
TC011100 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid105: 394040..405170
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011100
    iBeetle screen iB_01803
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     head mostly remaining - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Cuticle ball with antennae, labrum, residues of maxillae und mandibles. No labium. 
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection

    Fertility
    egg production: number no/almost no eggs -
    mature egg not present - Number of animals showing the phenotype: 4

    tropharium size decreased - Number of animals showing the phenotype: 4

    vitellarium sometimes shortened - Number of animals showing the phenotype: 4
    egg chamber number decreased - Number of animals showing the phenotype: 4

    vitellarium sometimes shape irregular - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011100 RA
      MSTRIKRKH RMSFWDKVD MEVLEATSE TFRIEVDKA 
FQTFLADGG AKEYSFPTN LNNFERKHI HQKAASYNL 
VSRSHGKDP NRVITIYKK GAKSNFESF YIDLTPETV 
RELDKTLQV VGMSPMGAP PLRWTCRDK VFGKLSLGV 
PPPVTAPRL TPQIAAFRK QLPIYDLRQ VIVSKVRKN 
RVLIVSSET GSGKTTQIP QFIMDDALQ RGEPCRILC 
TQPRRISAV SVAERVSFE RGAPLGETV GYHIRLESK 
VGSNCNLIY CTNGVLVRS LMSGMGALG TLTHVIIDE 
VHERDKLSD FLLICLRES LRKGAPIKL LLMSATINV 
EKFQRYFEG AEVLAVPGR LFAIREFFL DDVLELTGY 
DAVVREKRN FFEGAVGEN PLFDEVIMR VLQGGIDNE 
QNWAQLLQF LLTDMPVNY RHSENGATA LMAAVSQDN 
VDMVEKLCS LGADPHIPS KSGQTALSI AENKHLEEI 
LLILNVTSR PLLPREDLL TNYYKTCAD EDINYDLLI 
RLIYTIHLQ YHTGAILVF LPGYDDIMI CNDRLLETN 
IDKNSYRVF FLHGSMNIK EQHEVFKPF PDNVRKIIL 
STNIAETSL TIDDVVYVV DCGKAKVQT YDSCSGLSS 
LQTQWISKA CVKQRAGRA GRTQSGVCF HLFSRTRYD 
SFLDERIPE ILRVPLEEL CLNTKSLAD NISIYNFLV 
MAPDPPSSN TVKTAIENL ECLGALDKE ERLTPLGEY 
LSQLTIEPH LGKMLIYSA IFKCLDPIL TIVASLAQK 
DPFQLPPQA NLRSFAAEK RKSLTANTY SDHMVYLKA 
FIKWQDSVK YRKERNFCR EFFISPSTM ETILKTRSQ 
LLGQLRAAK FVPSNGTSM VALNTNSEN WPLVKAVIS 
SGLYPKLAF KKGANFCTR TEKKVLVHN TSPMSKDLP 
LWLVYDEMV KVRNGHQVR GVTPVTPVT ISLMCGIKV 
TYPMQNYLE IDEWLEFEF PSPHIINLR RLIETIIEK 
KLLNPTNIL SENEELAMI ALSKILIYE DRAAKLEIP 
VGIRQKPRF FELNRALDY VENRGAFGG PSTSGAPNM 
APMHPQYEN SVFLLIKVK QECSVKIAF DSNKWIFSP 
QTEKQVMAL DNGGMNVYL VYTVQKLNA FYGIAKYLT 
FDTTRHKTS AAIEWIFKS CVPYKSVSH LRNALNNNR 
LIYDGLDGQ EIEMGAALQ LWDLYLGQK RQ
    CDS sequence
    • >TC011100 RA
      atgtccacg cgtatcaaa cgcaaacac aggatgtct 
ttctgggac aaagttgac atggaagtg cttgaagcc 
acgtctgaa acgttccga attgaagtg gataaggcc 
tttcagacg ttccttgca gatgggggc gctaaagaa 
tactcgttt ccgacgaat ttaaataat tttgaacgc 
aaacatatt catcagaaa gctgcaagt tataattta 
gtgtctcgg agtcatggc aaggacccc aatcgcgtt 
attacgatt tataagaaa ggggcaaag tcaaatttc 
gagtcgttt tacattgat ttaaccccg gaaactgta 
cgcgagctg gataagacg ctccaggtg gtggggatg 
tccccaatg ggggcgccc cctctccgg tggacttgc 
cgcgataaa gtcttcggg aagttgtcg ctgggggtt 
ccacccccg gtgacagcc ccccgactg actccccag 
atcgccgcc ttccgtaaa caactcccc atttacgat 
ttgcgacaa gtgattgtc tccaaagtg cgaaaaaat 
cgcgtcttg atcgtgtct tcggagaca ggttcgggg 
aaaaccacc caaatcccc caatttata atggatgat 
gccctccaa aggggcgaa ccgtgtcgt attttatgc 
acacaacca cgtcgcatt tccgccgtc tcggtcgcc 
gagagggtg tctttcgag cggggggcc cccttgggc 
gagacagtg gggtaccac atccgactc gagtcaaaa 
gtgggctcc aattgtaac ctaatctat tgcacgaat 
ggggttttg gtgcggagt ctcatgtcg ggcatgggg 
gccctgggg accctcacg cacgttatc atcgacgag 
gtgcacgaa cgggacaaa ctgtccgat tttttgctc 
atttgcttg agggagagt ctgaggaag ggggccccc 
attaagttg cttctgatg tctgccact attaacgtg 
gagaagttc cagcggtat tttgagggg gctgaggtg 
ttggcggtg ccggggagg ttgtttgcg attagggaa 
ttttttctg gatgatgtg ctggagttg actggctat 
gatgctgta gtgagggaa aaaaggaat ttttttgag 
ggggctgtc ggggagaat cccctgttt gatgaggtt 
attatgagg gtgctgcaa gggggcatt gacaatgag 
cagaattgg gcacaatta ttacagttt cttttgact 
gatatgccg gtgaattac cgacattcg gaaaatggg 
gcgacggct ttaatggca gctgtgagt caggataat 
gtcgatatg gtggagaag ttgtgcagt ttgggcgct 
gaccctcac atcccctca aaaagcggc cagaccgcg 
ctctccatc gccgaaaac aagcacctg gaggagatc 
ctcctcatc ctcaacgtg acctcccgt cctctgtta 
ccacgcgag gaccttctc acgaattat tacaaaact 
tgcgctgat gaagacatc aactatgac ctcctcatc 
cgtctgatt tacaccatc catcttcag taccacacc 
ggcgccatt ttggtgttt ttgccaggc tatgatgat 
ataatgatc tgtaatgat cgcctgttg gagaccaac 
attgataaa aactcatac cgggtgttt ttcctccac 
ggttcaatg aatatcaag gaacagcat gaggtgttt 
aaaccgttc cctgataat gtaaggaag attatttta 
tcgacgaat attgctgag acgagtttg accattgat 
gatgtggtt tatgttgtc gattgtggc aaagctaaa 
gtgcaaacg tatgactcg tgcagcggt ctctcctcc 
ctccaaacc caatggatc tccaaagcc tgcgtcaaa 
cagcgcgca ggtcgtgca ggtcgcacc caatcaggt 
gtctgcttc cacctcttc tcacgcact cgttacgac 
tcgttctta gacgaacgc attcccgaa atattacga 
gtaccactt gaggaactc tgccttaat actaaatct 
ctagctgat aacatatcc atctataac ttccttgta 
atggcgccc gatccgcca tcttccaac accgtcaaa 
accgcaatc gaaaatctc gaatgtctc ggcgcactt 
gataaagaa gaacgcctt acacctcta ggagaatat 
ctaagccaa ctgaccatc gaaccccat ttaggaaaa 
atgctcatc tattcggcc attttcaag tgtttggac 
ccgattttg acaattgtg gcatcgttg gcccaaaag 
gacccgttt cagctccca ccacaagcc aatcttcgg 
agttttgcc gctgaaaag cgcaaaagt ctcactgcc 
aacacctac agtgaccat atggtctac ctgaaggct 
ttcatcaaa tggcaggat tcggtaaag taccgaaaa 
gagcggaat ttttgccgc gaatttttc ataagtccc 
tccaccatg gagacaata cttaagaca aggtcgcaa 
cttttggga caattgcgt gctgctaag tttgtgccg 
tcgaatggt acttcaatg gtggccttg aatacgaat 
tccgagaat tggcccttg gttaaggct gttatttcg 
tcgggactt tacccgaaa ttggcgttt aagaaaggg 
gcgaatttt tgcacaagg acggaaaaa aaagtactg 
gtgcataat acgagtccg atgagtaag gatttgccc 
ttgtggttg gtttatgac gaaatggtc aaggtcagg 
aatgggcat caggttagg ggagtcaca ccggtaaca 
cctgttacg atatcgtta atgtgtggc ataaaagtc 
acctatcca atgcaaaat tatctcgaa attgatgaa 
tggctcgaa ttcgaattt ccctcccct cacattatc 
aatcttcgt cgtttaatc gaaacaata atcgaaaaa 
aaactcctc aaccccacc aacattctc agcgaaaac 
gaagagttg gcgatgatc gccctctcc aaaatcctg 
atttacgaa gacagggct gccaaactc gaaattccg 
gtgggaatt cgccaaaaa ccccgattt ttcgaactg 
aatcgagcg ctcgattac gtcgaaaat cggggcgct 
ttcggggga ccctccacc tcgggagcc ccaaatatg 
gcccccatg cacccccag tacgaaaat tccgttttt 
ttgctaatc aaagtgaag caggaatgc agcgttaaa 
atcgccttc gattcaaac aagtggatt ttttcgccg 
caaactgaa aaacaagtc atggcccta gataatggt 
ggtatgaat gtttatttg gtatatact gtgcaaaaa 
ttgaatgcg ttttatgga attgccaaa tatttgacg 
tttgacaca acacgtcat aaaacttcg gcggccatt 
gagtggatt ttcaagtct tgtgtgccg tacaagagt 
gtcagccat ttgaggaac gctctgaac aataatcgc 
ttgatctac gatggcttg gacgggcag gagatagag 
atgggagcg gcgctgcaa ttatgggac ctctatctt 
ggtcaaaaa agacagtga
    mRNA sequence
    • >TC011100 RA
      tatataatg acagcaaaa aataaggtt atgtaacgt 
tcttttttg cccaaaaac tggaaaaaa ggggcccag 
accccccca aaaaaatgt ccacgcgta tcaaacgca 
aacacagga tgtctttct gggacaaag ttgacatgg 
aagtgcttg aagccacgt ctgaaacgt tccgaattg 
aagtggata aggcctttc agacgttcc ttgcagatg 
ggggcgcta aagaatact cgtttccga cgaatttaa 
ataattttg aacgcaaac atattcatc agaaagctg 
caagttata atttagtgt ctcggagtc atggcaagg 
accccaatc gcgttatta cgatttata agaaagggg 
caaagtcaa atttcgagt cgttttaca ttgatttaa 
ccccggaaa ctgtacgcg agctggata agacgctcc 
aggtggtgg ggatgtccc caatggggg cgccccctc 
tccggtgga cttgccgcg ataaagtct tcgggaagt 
tgtcgctgg gggttccac ccccggtga cagcccccc 
gactgactc cccagatcg ccgccttcc gtaaacaac 
tccccattt acgatttgc gacaagtga ttgtctcca 
aagtgcgaa aaaatcgcg tcttgatcg tgtcttcgg 
agacaggtt cggggaaaa ccacccaaa tcccccaat 
ttataatgg atgatgccc tccaaaggg gcgaaccgt 
gtcgtattt tatgcacac aaccacgtc gcatttccg 
ccgtctcgg tcgccgaga gggtgtctt tcgagcggg 
gggccccct tgggcgaga cagtggggt accacatcc 
gactcgagt caaaagtgg gctccaatt gtaacctaa 
tctattgca cgaatgggg ttttggtgc ggagtctca 
tgtcgggca tgggggccc tggggaccc tcacgcacg 
ttatcatcg acgaggtgc acgaacggg acaaactgt 
ccgattttt tgctcattt gcttgaggg agagtctga 
ggaaggggg cccccatta agttgcttc tgatgtctg 
ccactatta acgtggaga agttccagc ggtattttg 
agggggctg aggtgttgg cggtgccgg ggaggttgt 
ttgcgatta gggaatttt ttctggatg atgtgctgg 
agttgactg gctatgatg ctgtagtga gggaaaaaa 
ggaattttt ttgaggggg ctgtcgggg agaatcccc 
tgtttgatg aggttatta tgagggtgc tgcaagggg 
gcattgaca atgagcaga attgggcac aattattac 
agtttcttt tgactgata tgccggtga attaccgac 
attcggaaa atggggcga cggctttaa tggcagctg 
tgagtcagg ataatgtcg atatggtgg agaagttgt 
gcagtttgg gcgctgacc ctcacatcc cctcaaaaa 
gcggccaga ccgcgctct ccatcgccg aaaacaagc 
acctggagg agatcctcc tcatcctca acgtgacct 
cccgtcctc tgttaccac gcgaggacc ttctcacga 
attattaca aaacttgcg ctgatgaag acatcaact 
atgacctcc tcatccgtc tgatttaca ccatccatc 
ttcagtacc acaccggcg ccattttgg tgtttttgc 
caggctatg atgatataa tgatctgta atgatcgcc 
tgttggaga ccaacattg ataaaaact cataccggg 
tgtttttcc tccacggtt caatgaata tcaaggaac 
agcatgagg tgtttaaac cgttccctg ataatgtaa 
ggaagatta ttttatcga cgaatattg ctgagacga 
gtttgacca ttgatgatg tggtttatg ttgtcgatt 
gtggcaaag ctaaagtgc aaacgtatg actcgtgca 
gcggtctct cctccctcc aaacccaat ggatctcca 
aagcctgcg tcaaacagc gcgcaggtc gtgcaggtc 
gcacccaat caggtgtct gcttccacc tcttctcac 
gcactcgtt acgactcgt tcttagacg aacgcattc 
ccgaaatat tacgagtac cacttgagg aactctgcc 
ttaatacta aatctctag ctgataaca tatccatct 
ataacttcc ttgtaatgg cgcccgatc cgccatctt 
ccaacaccg tcaaaaccg caatcgaaa atctcgaat 
gtctcggcg cacttgata aagaagaac gccttacac 
ctctaggag aatatctaa gccaactga ccatcgaac 
cccatttag gaaaaatgc tcatctatt cggccattt 
tcaagtgtt tggacccga ttttgacaa ttgtggcat 
cgttggccc aaaaggacc cgtttcagc tcccaccac 
aagccaatc ttcggagtt ttgccgctg aaaagcgca 
aaagtctca ctgccaaca cctacagtg accatatgg 
tctacctga aggctttca tcaaatggc aggattcgg 
taaagtacc gaaaagagc ggaattttt gccgcgaat 
ttttcataa gtccctcca ccatggaga caatactta 
agacaaggt cgcaacttt tgggacaat tgcgtgctg 
ctaagtttg tgccgtcga atggtactt caatggtgg 
ccttgaata cgaattccg agaattggc ccttggtta 
aggctgtta tttcgtcgg gactttacc cgaaattgg 
cgtttaaga aaggggcga atttttgca caaggacgg 
aaaaaaaag tactggtgc ataatacga gtccgatga 
gtaaggatt tgcccttgt ggttggttt atgacgaaa 
tggtcaagg tcaggaatg ggcatcagg ttaggggag 
tcacaccgg taacacctg ttacgatat cgttaatgt 
gtggcataa aagtcacct atccaatgc aaaattatc 
tcgaaattg atgaatggc tcgaattcg aatttccct 
cccctcaca ttatcaatc ttcgtcgtt taatcgaaa 
caataatcg aaaaaaaac tcctcaacc ccaccaaca 
ttctcagcg aaaacgaag agttggcga tgatcgccc 
tctccaaaa tcctgattt acgaagaca gggctgcca 
aactcgaaa ttccggtgg gaattcgcc aaaaacccc 
gatttttcg aactgaatc gagcgctcg attacgtcg 
aaaatcggg gcgctttcg ggggaccct ccacctcgg 
gagccccaa atatggccc ccatgcacc cccagtacg 
aaaattccg tttttttgc taatcaaag tgaagcagg 
aatgcagcg ttaaaatcg ccttcgatt caaacaagt 
ggatttttt cgccgcaaa ctgaaaaac aagtcatgg 
ccctagata atggtggta tgaatgttt atttggtat 
atactgtgc aaaaattga atgcgtttt atggaattg 
ccaaatatt tgacgtttg acacaacac gtcataaaa 
cttcggcgg ccattgagt ggattttca agtcttgtg 
tgccgtaca agagtgtca gccatttga ggaacgctc 
tgaacaata atcgcttga tctacgatg gcttggacg 
ggcaggaga tagagatgg gagcggcgc tgcaattat 
gggacctct atcttggtc aaaaaagac agtgatttt 
ttcaagtaa ctgtgttag aagtattac attttttta 
atttgatgt tgtgatatt gatttatgt ttgcgattt 
ttagaagat gtatttttt tgtataaat gagtaacga 
cgtaccttt taggataat aaagtttct ttatttcg