Status
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Gene information
General information
TC011100 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 394039..405169
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011100
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01803

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    head mostly remaining - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Cuticle ball with antennae, labrum, residues of maxillae und mandibles. No labium. 
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    mature egg not present - Number of animals showing the phenotype: 4

    tropharium size decreased - Number of animals showing the phenotype: 4

    vitellarium sometimes shortened - Number of animals showing the phenotype: 4
    egg chamber number decreased - Number of animals showing the phenotype: 4

    vitellarium sometimes shape irregular - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011100 RA
      MSTRIKRKH RMSFWDKVD MEVLEATSE TFRIEVDKA 
      FQTFLADGG AKEYSFPTN LNNFERKHI HQKAASYNL 
      VSRSHGKDP NRVITIYKK GAKSNFESF YIDLTPETV 
      RELDKTLQV VGMSPMGAP PLRWTCRDK VFGKLSLGV 
      PPPVTAPRL TPQIAAFRK QLPIYDLRQ VIVSKVRKN 
      RVLIVSSET GSGKTTQIP QFIMDDALQ RGEPCRILC 
      TQPRRISAV SVAERVSFE RGAPLGETV GYHIRLESK 
      VGSNCNLIY CTNGVLVRS LMSGMGALG TLTHVIIDE 
      VHERDKLSD FLLICLRES LRKGAPIKL LLMSATINV 
      EKFQRYFEG AEVLAVPGR LFAIREFFL DDVLELTGY 
      DAVVREKRN FFEGAVGEN PLFDEVIMR VLQGGIDNE 
      QNWAQLLQF LLTDMPVNY RHSENGATA LMAAVSQDN 
      VDMVEKLCS LGADPHIPS KSGQTALSI AENKHLEEI 
      LLILNVTSR PLLPREDLL TNYYKTCAD EDINYDLLI 
      RLIYTIHLQ YHTGAILVF LPGYDDIMI CNDRLLETN 
      IDKNSYRVF FLHGSMNIK EQHEVFKPF PDNVRKIIL 
      STNIAETSL TIDDVVYVV DCGKAKVQT YDSCSGLSS 
      LQTQWISKA CVKQRAGRA GRTQSGVCF HLFSRTRYD 
      SFLDERIPE ILRVPLEEL CLNTKSLAD NISIYNFLV 
      MAPDPPSSN TVKTAIENL ECLGALDKE ERLTPLGEY 
      LSQLTIEPH LGKMLIYSA IFKCLDPIL TIVASLAQK 
      DPFQLPPQA NLRSFAAEK RKSLTANTY SDHMVYLKA 
      FIKWQDSVK YRKERNFCR EFFISPSTM ETILKTRSQ 
      LLGQLRAAK FVPSNGTSM VALNTNSEN WPLVKAVIS 
      SGLYPKLAF KKGANFCTR TEKKVLVHN TSPMSKDLP 
      LWLVYDEMV KVRNGHQVR GVTPVTPVT ISLMCGIKV 
      TYPMQNYLE IDEWLEFEF PSPHIINLR RLIETIIEK 
      KLLNPTNIL SENEELAMI ALSKILIYE DRAAKLEIP 
      VGIRQKPRF FELNRALDY VENRGAFGG PSTSGAPNM 
      APMHPQYEN SVFLLIKVK QECSVKIAF DSNKWIFSP 
      QTEKQVMAL DNGGMNVYL VYTVQKLNA FYGIAKYLT 
      FDTTRHKTS AAIEWIFKS CVPYKSVSH LRNALNNNR 
      LIYDGLDGQ EIEMGAALQ LWDLYLGQK RQ
    CDS sequence
    • >TC011100 RA
      atgtccacg cgtatcaaa cgcaaacac aggatgtct 
      ttctgggac aaagttgac atggaagtg cttgaagcc 
      acgtctgaa acgttccga attgaagtg gataaggcc 
      tttcagacg ttccttgca gatgggggc gctaaagaa 
      tactcgttt ccgacgaat ttaaataat tttgaacgc 
      aaacatatt catcagaaa gctgcaagt tataattta 
      gtgtctcgg agtcatggc aaggacccc aatcgcgtt 
      attacgatt tataagaaa ggggcaaag tcaaatttc 
      gagtcgttt tacattgat ttaaccccg gaaactgta 
      cgcgagctg gataagacg ctccaggtg gtggggatg 
      tccccaatg ggggcgccc cctctccgg tggacttgc 
      cgcgataaa gtcttcggg aagttgtcg ctgggggtt 
      ccacccccg gtgacagcc ccccgactg actccccag 
      atcgccgcc ttccgtaaa caactcccc atttacgat 
      ttgcgacaa gtgattgtc tccaaagtg cgaaaaaat 
      cgcgtcttg atcgtgtct tcggagaca ggttcgggg 
      aaaaccacc caaatcccc caatttata atggatgat 
      gccctccaa aggggcgaa ccgtgtcgt attttatgc 
      acacaacca cgtcgcatt tccgccgtc tcggtcgcc 
      gagagggtg tctttcgag cggggggcc cccttgggc 
      gagacagtg gggtaccac atccgactc gagtcaaaa 
      gtgggctcc aattgtaac ctaatctat tgcacgaat 
      ggggttttg gtgcggagt ctcatgtcg ggcatgggg 
      gccctgggg accctcacg cacgttatc atcgacgag 
      gtgcacgaa cgggacaaa ctgtccgat tttttgctc 
      atttgcttg agggagagt ctgaggaag ggggccccc 
      attaagttg cttctgatg tctgccact attaacgtg 
      gagaagttc cagcggtat tttgagggg gctgaggtg 
      ttggcggtg ccggggagg ttgtttgcg attagggaa 
      ttttttctg gatgatgtg ctggagttg actggctat 
      gatgctgta gtgagggaa aaaaggaat ttttttgag 
      ggggctgtc ggggagaat cccctgttt gatgaggtt 
      attatgagg gtgctgcaa gggggcatt gacaatgag 
      cagaattgg gcacaatta ttacagttt cttttgact 
      gatatgccg gtgaattac cgacattcg gaaaatggg 
      gcgacggct ttaatggca gctgtgagt caggataat 
      gtcgatatg gtggagaag ttgtgcagt ttgggcgct 
      gaccctcac atcccctca aaaagcggc cagaccgcg 
      ctctccatc gccgaaaac aagcacctg gaggagatc 
      ctcctcatc ctcaacgtg acctcccgt cctctgtta 
      ccacgcgag gaccttctc acgaattat tacaaaact 
      tgcgctgat gaagacatc aactatgac ctcctcatc 
      cgtctgatt tacaccatc catcttcag taccacacc 
      ggcgccatt ttggtgttt ttgccaggc tatgatgat 
      ataatgatc tgtaatgat cgcctgttg gagaccaac 
      attgataaa aactcatac cgggtgttt ttcctccac 
      ggttcaatg aatatcaag gaacagcat gaggtgttt 
      aaaccgttc cctgataat gtaaggaag attatttta 
      tcgacgaat attgctgag acgagtttg accattgat 
      gatgtggtt tatgttgtc gattgtggc aaagctaaa 
      gtgcaaacg tatgactcg tgcagcggt ctctcctcc 
      ctccaaacc caatggatc tccaaagcc tgcgtcaaa 
      cagcgcgca ggtcgtgca ggtcgcacc caatcaggt 
      gtctgcttc cacctcttc tcacgcact cgttacgac 
      tcgttctta gacgaacgc attcccgaa atattacga 
      gtaccactt gaggaactc tgccttaat actaaatct 
      ctagctgat aacatatcc atctataac ttccttgta 
      atggcgccc gatccgcca tcttccaac accgtcaaa 
      accgcaatc gaaaatctc gaatgtctc ggcgcactt 
      gataaagaa gaacgcctt acacctcta ggagaatat 
      ctaagccaa ctgaccatc gaaccccat ttaggaaaa 
      atgctcatc tattcggcc attttcaag tgtttggac 
      ccgattttg acaattgtg gcatcgttg gcccaaaag 
      gacccgttt cagctccca ccacaagcc aatcttcgg 
      agttttgcc gctgaaaag cgcaaaagt ctcactgcc 
      aacacctac agtgaccat atggtctac ctgaaggct 
      ttcatcaaa tggcaggat tcggtaaag taccgaaaa 
      gagcggaat ttttgccgc gaatttttc ataagtccc 
      tccaccatg gagacaata cttaagaca aggtcgcaa 
      cttttggga caattgcgt gctgctaag tttgtgccg 
      tcgaatggt acttcaatg gtggccttg aatacgaat 
      tccgagaat tggcccttg gttaaggct gttatttcg 
      tcgggactt tacccgaaa ttggcgttt aagaaaggg 
      gcgaatttt tgcacaagg acggaaaaa aaagtactg 
      gtgcataat acgagtccg atgagtaag gatttgccc 
      ttgtggttg gtttatgac gaaatggtc aaggtcagg 
      aatgggcat caggttagg ggagtcaca ccggtaaca 
      cctgttacg atatcgtta atgtgtggc ataaaagtc 
      acctatcca atgcaaaat tatctcgaa attgatgaa 
      tggctcgaa ttcgaattt ccctcccct cacattatc 
      aatcttcgt cgtttaatc gaaacaata atcgaaaaa 
      aaactcctc aaccccacc aacattctc agcgaaaac 
      gaagagttg gcgatgatc gccctctcc aaaatcctg 
      atttacgaa gacagggct gccaaactc gaaattccg 
      gtgggaatt cgccaaaaa ccccgattt ttcgaactg 
      aatcgagcg ctcgattac gtcgaaaat cggggcgct 
      ttcggggga ccctccacc tcgggagcc ccaaatatg 
      gcccccatg cacccccag tacgaaaat tccgttttt 
      ttgctaatc aaagtgaag caggaatgc agcgttaaa 
      atcgccttc gattcaaac aagtggatt ttttcgccg 
      caaactgaa aaacaagtc atggcccta gataatggt 
      ggtatgaat gtttatttg gtatatact gtgcaaaaa 
      ttgaatgcg ttttatgga attgccaaa tatttgacg 
      tttgacaca acacgtcat aaaacttcg gcggccatt 
      gagtggatt ttcaagtct tgtgtgccg tacaagagt 
      gtcagccat ttgaggaac gctctgaac aataatcgc 
      ttgatctac gatggcttg gacgggcag gagatagag 
      atgggagcg gcgctgcaa ttatgggac ctctatctt 
      ggtcaaaaa agacagtga
    mRNA sequence
    • >TC011100 RA
      tatataatg acagcaaaa aataaggtt atgtaacgt 
      tcttttttg cccaaaaac tggaaaaaa ggggcccag 
      accccccca aaaaaatgt ccacgcgta tcaaacgca 
      aacacagga tgtctttct gggacaaag ttgacatgg 
      aagtgcttg aagccacgt ctgaaacgt tccgaattg 
      aagtggata aggcctttc agacgttcc ttgcagatg 
      ggggcgcta aagaatact cgtttccga cgaatttaa 
      ataattttg aacgcaaac atattcatc agaaagctg 
      caagttata atttagtgt ctcggagtc atggcaagg 
      accccaatc gcgttatta cgatttata agaaagggg 
      caaagtcaa atttcgagt cgttttaca ttgatttaa 
      ccccggaaa ctgtacgcg agctggata agacgctcc 
      aggtggtgg ggatgtccc caatggggg cgccccctc 
      tccggtgga cttgccgcg ataaagtct tcgggaagt 
      tgtcgctgg gggttccac ccccggtga cagcccccc 
      gactgactc cccagatcg ccgccttcc gtaaacaac 
      tccccattt acgatttgc gacaagtga ttgtctcca 
      aagtgcgaa aaaatcgcg tcttgatcg tgtcttcgg 
      agacaggtt cggggaaaa ccacccaaa tcccccaat 
      ttataatgg atgatgccc tccaaaggg gcgaaccgt 
      gtcgtattt tatgcacac aaccacgtc gcatttccg 
      ccgtctcgg tcgccgaga gggtgtctt tcgagcggg 
      gggccccct tgggcgaga cagtggggt accacatcc 
      gactcgagt caaaagtgg gctccaatt gtaacctaa 
      tctattgca cgaatgggg ttttggtgc ggagtctca 
      tgtcgggca tgggggccc tggggaccc tcacgcacg 
      ttatcatcg acgaggtgc acgaacggg acaaactgt 
      ccgattttt tgctcattt gcttgaggg agagtctga 
      ggaaggggg cccccatta agttgcttc tgatgtctg 
      ccactatta acgtggaga agttccagc ggtattttg 
      agggggctg aggtgttgg cggtgccgg ggaggttgt 
      ttgcgatta gggaatttt ttctggatg atgtgctgg 
      agttgactg gctatgatg ctgtagtga gggaaaaaa 
      ggaattttt ttgaggggg ctgtcgggg agaatcccc 
      tgtttgatg aggttatta tgagggtgc tgcaagggg 
      gcattgaca atgagcaga attgggcac aattattac 
      agtttcttt tgactgata tgccggtga attaccgac 
      attcggaaa atggggcga cggctttaa tggcagctg 
      tgagtcagg ataatgtcg atatggtgg agaagttgt 
      gcagtttgg gcgctgacc ctcacatcc cctcaaaaa 
      gcggccaga ccgcgctct ccatcgccg aaaacaagc 
      acctggagg agatcctcc tcatcctca acgtgacct 
      cccgtcctc tgttaccac gcgaggacc ttctcacga 
      attattaca aaacttgcg ctgatgaag acatcaact 
      atgacctcc tcatccgtc tgatttaca ccatccatc 
      ttcagtacc acaccggcg ccattttgg tgtttttgc 
      caggctatg atgatataa tgatctgta atgatcgcc 
      tgttggaga ccaacattg ataaaaact cataccggg 
      tgtttttcc tccacggtt caatgaata tcaaggaac 
      agcatgagg tgtttaaac cgttccctg ataatgtaa 
      ggaagatta ttttatcga cgaatattg ctgagacga 
      gtttgacca ttgatgatg tggtttatg ttgtcgatt 
      gtggcaaag ctaaagtgc aaacgtatg actcgtgca 
      gcggtctct cctccctcc aaacccaat ggatctcca 
      aagcctgcg tcaaacagc gcgcaggtc gtgcaggtc 
      gcacccaat caggtgtct gcttccacc tcttctcac 
      gcactcgtt acgactcgt tcttagacg aacgcattc 
      ccgaaatat tacgagtac cacttgagg aactctgcc 
      ttaatacta aatctctag ctgataaca tatccatct 
      ataacttcc ttgtaatgg cgcccgatc cgccatctt 
      ccaacaccg tcaaaaccg caatcgaaa atctcgaat 
      gtctcggcg cacttgata aagaagaac gccttacac 
      ctctaggag aatatctaa gccaactga ccatcgaac 
      cccatttag gaaaaatgc tcatctatt cggccattt 
      tcaagtgtt tggacccga ttttgacaa ttgtggcat 
      cgttggccc aaaaggacc cgtttcagc tcccaccac 
      aagccaatc ttcggagtt ttgccgctg aaaagcgca 
      aaagtctca ctgccaaca cctacagtg accatatgg 
      tctacctga aggctttca tcaaatggc aggattcgg 
      taaagtacc gaaaagagc ggaattttt gccgcgaat 
      ttttcataa gtccctcca ccatggaga caatactta 
      agacaaggt cgcaacttt tgggacaat tgcgtgctg 
      ctaagtttg tgccgtcga atggtactt caatggtgg 
      ccttgaata cgaattccg agaattggc ccttggtta 
      aggctgtta tttcgtcgg gactttacc cgaaattgg 
      cgtttaaga aaggggcga atttttgca caaggacgg 
      aaaaaaaag tactggtgc ataatacga gtccgatga 
      gtaaggatt tgcccttgt ggttggttt atgacgaaa 
      tggtcaagg tcaggaatg ggcatcagg ttaggggag 
      tcacaccgg taacacctg ttacgatat cgttaatgt 
      gtggcataa aagtcacct atccaatgc aaaattatc 
      tcgaaattg atgaatggc tcgaattcg aatttccct 
      cccctcaca ttatcaatc ttcgtcgtt taatcgaaa 
      caataatcg aaaaaaaac tcctcaacc ccaccaaca 
      ttctcagcg aaaacgaag agttggcga tgatcgccc 
      tctccaaaa tcctgattt acgaagaca gggctgcca 
      aactcgaaa ttccggtgg gaattcgcc aaaaacccc 
      gatttttcg aactgaatc gagcgctcg attacgtcg 
      aaaatcggg gcgctttcg ggggaccct ccacctcgg 
      gagccccaa atatggccc ccatgcacc cccagtacg 
      aaaattccg tttttttgc taatcaaag tgaagcagg 
      aatgcagcg ttaaaatcg ccttcgatt caaacaagt 
      ggatttttt cgccgcaaa ctgaaaaac aagtcatgg 
      ccctagata atggtggta tgaatgttt atttggtat 
      atactgtgc aaaaattga atgcgtttt atggaattg 
      ccaaatatt tgacgtttg acacaacac gtcataaaa 
      cttcggcgg ccattgagt ggattttca agtcttgtg 
      tgccgtaca agagtgtca gccatttga ggaacgctc 
      tgaacaata atcgcttga tctacgatg gcttggacg 
      ggcaggaga tagagatgg gagcggcgc tgcaattat 
      gggacctct atcttggtc aaaaaagac agtgatttt 
      ttcaagtaa ctgtgttag aagtattac attttttta 
      atttgatgt tgtgatatt gatttatgt ttgcgattt 
      ttagaagat gtatttttt tgtataaat gagtaacga 
      cgtaccttt taggataat aaagtttct ttatttcg
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