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Gene information
General information
TC011087 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 591593..606969
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011087
    iBeetle screen iB_01801

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011087 RA
      MATPIVSLH PMPISIATP SRDSMTFTS TKGLLNGPG 
      QNNCFLNSA VQVLWHLDI FRRSFRDLS GHACMADSC 
      IFCALKELF SQLQFSNET ALPPDALRR ALAESFFDQ 
      QRFQLGFMD DAAECFENM LLRIHMHIA HGEAEDMCN 
      ARHCIPHQK FAMTLVEQS VCNSCGATS EPLSYTQMV 
      HYVSTSTLV YQVRTTRPQ GIIEPFGQL LRKAGSMGD 
      IRVCPSACG AVIQIGRTL MNRPEIVSV GLVWNSERP 
      TLEHIMEVF STIGTTLRL GEVFNSVID SRWAETSVH 
      TLVGVVTYY GKHYSTFFF HTKLRVWIY FDDATVREV 
      GPRWDQVVD KCRKGRYQP LLLLYALPD GTPVNTENA 
      PKQVVPFYD KPKKLGLRR SVTPSPEKP IIGTTRRAI 
      TPNPDGTTC QKPPLPRSY NEYQNLSVI QSNLYPEPH 
      RNIHRTLSN GSSSGTEGL FRDYPNVPR TRDSGNWSS 
      DRNSASSSS STTMENPYL YLVGKVPEP TQRNESSSS 
      TSSGVYDVG YDSYSLSSN DSSTMTTIQ HMMKMGHLA 
      KIPEDYNPG GQAQVSQSC DVLCDEADE LLVKSRQLE 
      DDHDLVLAL ALCNAAATK ARAAMDAPY NNPQTLTLA 
      RMKHNTCIM RARSLHRRM TQNQAQNKD VPPEIRHTR 
      EGSSGSCGK HSRQSSHSR QNSKEIVEK PAKNIEIYA 
      TLPKKKKVV SIEDQEEVL YDKPPGRES RSIFSRKNK 
      EKRSRSEDR NKVTKELSI APEPPKKEV KTKKQHKIR 
      RKLLMGGLI RRKNRSMPD LTDTCEKEK PTTTVDDSR 
      VGLNGGSTT DMCGYLSEG HLEFTNPNL ERSKLMRKS 
      FHGSAGKIL TAAKVPPPP PLRTTSQLT TNKEYYPLY 
      HNLVVTRAD VHQEGVDEV DCAPPSNLD LPPYPSPLG 
      SVIHSRQAS EEFPPPPPP LDLSALDEH LKPPTPPPT 
      PPSTLLAEL QVKRQEILA PGDTWLKEL QAKQAALRK 
      KQESVGSPG TSVRDLASK FENIQVFEG QNGVVTARK 
      RDDFDIPPE IPDEKRGKK KSVSFCDQV ILVATAEEQ 
      EDDSYIPNP ILERVLRSA MNKPETAAI RQEVRTLRQ 
      EMSPEPFVR RNSMDNLLS RQAPPPDPL YYNNYAQSP 
      YQRIPQNSP VYNAPPSYP QYQYVRPPV YQHPPSPRP 
      VYQERVPPL HPEYTPYQP IPRQLLRKS VSFEPGTKG 
      GAESPTPKA VVTPIVVNN ANNSVPTGK TKCNLCRKK 
      AVVSANLYC TDCEFYMSR FKPKR
    CDS sequence
    • >TC011087 RA
      atggcaacg ccaattgtt tcgctacat cccatgccc 
      atatccata gccacccct tcaagagac tcgatgacg 
      tttacgagc accaagggg ctgctaaat ggaccagga 
      cagaataat tgtttccta aatagtgca gttcaggtc 
      ctctggcat ttggacatt ttccggcgc agtttccgg 
      gacctttcc gggcacgct tgcatggcg gattcttgc 
      atcttctgc gccttaaaa gaactcttc tcccagtta 
      caattctcc aatgagact gctctccct cccgacgct 
      ttgcgtcga gctcttgct gagagcttc ttcgaccaa 
      cagcgcttc cagctgggc ttcatggac gacgcagcc 
      gaatgtttc gaaaatatg ctcctcaga atccacatg 
      catatagcc catggtgaa gccgaagat atgtgtaat 
      gcccgccat tgtatcccc catcaaaag ttcgcaatg 
      acactagtc gaacaaagt gtatgcaac tcgtgcggc 
      gccacctcc gagccgctt tcctacacc cagatggtc 
      cattacgtg tccacgtct accttggtg taccaagtg 
      cgtacgacg aggcctcaa ggcattatc gaacctttt 
      gggcaactt ttacgcaag gctgggagc atgggcgac 
      ataagggtg tgtccgagt gcgtgtggt gctgttatc 
      caaatcggc cgtacttta atgaaccgg cctgaaatc 
      gtctcagtt ggcttggta tggaattcg gaacggcct 
      actttggag cacataatg gaggtgttc tcgaccatt 
      ggtaccacc ctccgactg ggggaagtt ttcaacagt 
      gttatcgac agtcgttgg gctgaaact tccgtccat 
      actttagtc ggagttgtg acttattat ggcaaacac 
      tactcaacg ttctttttc cataccaag ctgcgagta 
      tggatttat ttcgacgat gctacagtt cgtgaagtg 
      ggaccacgg tgggaccaa gtggttgat aagtgtcgc 
      aaagggcgg taccaaccc cttctccta ctttacgcc 
      cttccggat ggtactcct gttaataca gaaaacgcc 
      ccaaaacaa gtggtacca ttctacgac aagccgaaa 
      aaattgggt ttgcgacgt tcggtgact ccaagtccc 
      gaaaaacca atcattggt accacccga cgggccata 
      accccgaac cctgatggt accacttgc cagaaaccc 
      cccctacca aggagttac aacgagtac cagaactta 
      tccgtgatt cagagtaat ctctacccg gaacctcac 
      cgtaacatc cacagaacc ctaagtaat ggttcatcg 
      agtggtacc gaaggcttg ttccgtgat tacccgaac 
      gttcctcga acccgtgat tcgggaaac tggtcaagt 
      gaccgcaac agtgcctcc tcctcctcc tccacgacc 
      atggagaac ccctacttg tacctcgtg ggtaaagta 
      ccggaaccc acccaacga aacgaatca tcgagtagt 
      accagcagt ggggtttac gacgtcggg tacgactcg 
      tactccctc tcctccaac gatagtagt accatgacc 
      accatccaa cacatgatg aagatgggt catttggcc 
      aaaatcccg gaggattac aacccgggt gggcaggcg 
      caagtgtcg caaagttgc gacgttttg tgcgacgaa 
      gccgacgag cttttggtc aaatcgcgc caattggaa 
      gatgaccat gaccttgtc cttgccttg gctttgtgt 
      aatgcagct gccaccaaa gccagagcc gccatggac 
      gccccttac aacaacccc caaacgctg accttggcc 
      cgcatgaag cacaacact tgcataatg cgtgctaga 
      agcctccac agacgaatg acccaaaac caggcccaa 
      aacaaggac gtccctccc gaaatcaga cacacacgg 
      gaggggagt tcggggtcc tgtgggaaa cactcccgt 
      caaagttca cactccagg cagaacagt aaggagatt 
      gtggagaaa ccggccaaa aatatcgag atttacgcc 
      actttgccc aaaaagaag aaagttgtg agcattgaa 
      gaccaggag gaggttttg tacgataaa cccccaggt 
      cgggagagt cgtagcatc ttctcccgc aagaacaag 
      gagaagcgt tcgaggtcc gaagaccgc aacaaagtc 
      acaaaggag ttgtctatt gcccccgaa ccccccaaa 
      aaggaggtt aagacaaag aaacaacac aagatcaga 
      cggaaactt ctgatgggg gggctaatc aggcgaaag 
      aatcgcagt atgcctgat ttaaccgac acttgtgag 
      aaggaaaaa cccacgacc acagttgat gacagtagg 
      gttggcctg aacggtggt agtaccacc gatatgtgt 
      gggtactta tcggaaggt cacctggag ttcaccaac 
      cccaacctg gaacgcagc aaattgatg cggaagagt 
      ttccatggt agtgctggt aagattctg accgcggct 
      aaagtacca ccccctcca ccgttacgt accacctcc 
      caactgacg accaataag gagtattac cccctctac 
      cacaacctg gtcgtgaca cgagccgac gtccatcaa 
      gagggcgtg gacgaagtc gattgtgcc cctccttca 
      aacctcgac ctcccccct tatcccagc ccactaggc 
      tcagtgata cactcacgc caagctagt gaagaattc 
      cccccacca ccaccccca cttgacttg tccgcattg 
      gacgaacac ttgaaaccc ccaactccg ccccccacc 
      ccaccctca acccttcta gctgagctg caagtcaaa 
      cgacaggag atcctagca cctggtgac acctggctg 
      aaggaactc caagccaaa caagccgcc ttaagaaag 
      aaacaagag agtgtgggt tcccccggg acgtcagtc 
      cgcgacttg gcctcaaaa ttcgaaaac atccaggtg 
      tttgagggc caaaatggc gtcgtcaca gcccggaaa 
      cgcgacgat tttgacatc ccgcccgaa attcccgac 
      gaaaaacgc gggaaaaag aaaagtgtc tcgttctgc 
      gaccaagtg atcctagtt gccacagcc gaggagcag 
      gaagacgac agttacatc ccgaacccg atcctggaa 
      cgcgtcctg cgctcagcc atgaacaag ccggaaacg 
      gccgccatt cgccaggaa gtccggact ttacgccag 
      gagatgtcc ccggagccg ttcgtgcgt cgcaactca 
      atggacaac ctcctctcg cggcaagcc ccgccccct 
      gaccccctc tactacaac aactacgcc cagtcgccc 
      taccaacga atcccgcag aactcgcca gtttacaac 
      gccccgccc tcctacccc cagtaccag tatgtccgg 
      ccgccggtg tatcaacat ccgccgtcg ccacgcccc 
      gtctatcaa gagcgcgtc ccgccgctc caccccgag 
      tacacgccc taccaacca atcccgcga cagttgcta 
      aggaaaagt gtgagtttt gagccgggg acgaaaggc 
      ggggcggag tcgcccact cccaaggcg gtggtcacg 
      cccattgtg gtcaataac gccaataat agcgtgccc 
      acggggaag accaagtgt aatctgtgt aggaagaag 
      gcggtggtc tcggccaac ttgtactgc acggattgc 
      gagttttac atgtcgagg tttaagccg aaaaggtag
    mRNA sequence
    • >TC011087 RA
      tagcaaacg cttgatggg acagcatgt agagaagac 
      ggcgccatg gcaacgcca attgtttcg ctacatccc 
      atgcccata tccatagcc accccttca agagactcg 
      atgacgttt acgagcacc aaggggctg ctaaatgga 
      ccaggacag aataattgt ttcctaaat agtgcagtt 
      caggtcctc tggcatttg gacattttc cggcgcagt 
      ttccgggac ctttccggg cacgcttgc atggcggat 
      tcttgcatc ttctgcgcc ttaaaagaa ctcttctcc 
      cagttacaa ttctccaat gagactgct ctccctccc 
      gacgctttg cgtcgagct cttgctgag agcttcttc 
      gaccaacag cgcttccag ctgggcttc atggacgac 
      gcagccgaa tgtttcgaa aatatgctc ctcagaatc 
      cacatgcat atagcccat ggtgaagcc gaagatatg 
      tgtaatgcc cgccattgt atcccccat caaaagttc 
      gcaatgaca ctagtcgaa caaagtgta tgcaactcg 
      tgcggcgcc acctccgag ccgctttcc tacacccag 
      atggtccat tacgtgtcc acgtctacc ttggtgtac 
      caagtgcgt acgacgagg cctcaaggc attatcgaa 
      ccttttggg caactttta cgcaaggct gggagcatg 
      ggcgacata agggtgtgt ccgagtgcg tgtggtgct 
      gttatccaa atcggccgt actttaatg aaccggcct 
      gaaatcgtc tcagttggc ttggtatgg aattcggaa 
      cggcctact ttggagcac ataatggag gtgttctcg 
      accattggt accaccctc cgactgggg gaagttttc 
      aacagtgtt atcgacagt cgttgggct gaaacttcc 
      gtccatact ttagtcgga gttgtgact tattatggc 
      aaacactac tcaacgttc tttttccat accaagctg 
      cgagtatgg atttatttc gacgatgct acagttcgt 
      gaagtggga ccacggtgg gaccaagtg gttgataag 
      tgtcgcaaa gggcggtac caacccctt ctcctactt 
      tacgccctt ccggatggt actcctgtt aatacagaa 
      aacgcccca aaacaagtg gtaccattc tacgacaag 
      ccgaaaaaa ttgggtttg cgacgttcg gtgactcca 
      agtcccgaa aaaccaatc attggtacc acccgacgg 
      gccataacc ccgaaccct gatggtacc acttgccag 
      aaacccccc ctaccaagg agttacaac gagtaccag 
      aacttatcc gtgattcag agtaatctc tacccggaa 
      cctcaccgt aacatccac agaacccta agtaatggt 
      tcatcgagt ggtaccgaa ggcttgttc cgtgattac 
      ccgaacgtt cctcgaacc cgtgattcg ggaaactgg 
      tcaagtgac cgcaacagt gcctcctcc tcctcctcc 
      acgaccatg gagaacccc tacttgtac ctcgtgggt 
      aaagtaccg gaacccacc caacgaaac gaatcatcg 
      agtagtacc agcagtggg gtttacgac gtcgggtac 
      gactcgtac tccctctcc tccaacgat agtagtacc 
      atgaccacc atccaacac atgatgaag atgggtcat 
      ttggccaaa atcccggag gattacaac ccgggtggg 
      caggcgcaa gtgtcgcaa agttgcgac gttttgtgc 
      gacgaagcc gacgagctt ttggtcaaa tcgcgccaa 
      ttggaagat gaccatgac cttgtcctt gccttggct 
      ttgtgtaat gcagctgcc accaaagcc agagccgcc 
      atggacgcc ccttacaac aacccccaa acgctgacc 
      ttggcccgc atgaagcac aacacttgc ataatgcgt 
      gctagaagc ctccacaga cgaatgacc caaaaccag 
      gcccaaaac aaggacgtc cctcccgaa atcagacac 
      acacgggag gggagttcg gggtcctgt gggaaacac 
      tcccgtcaa agttcacac tccaggcag aacagtaag 
      gagattgtg gagaaaccg gccaaaaat atcgagatt 
      tacgccact ttgcccaaa aagaagaaa gttgtgagc 
      attgaagac caggaggag gttttgtac gataaaccc 
      ccaggtcgg gagagtcgt agcatcttc tcccgcaag 
      aacaaggag aagcgttcg aggtccgaa gaccgcaac 
      aaagtcaca aaggagttg tctattgcc cccgaaccc 
      cccaaaaag gaggttaag acaaagaaa caacacaag 
      atcagacgg aaacttctg atggggggg ctaatcagg 
      cgaaagaat cgcagtatg cctgattta accgacact 
      tgtgagaag gaaaaaccc acgaccaca gttgatgac 
      agtagggtt ggcctgaac ggtggtagt accaccgat 
      atgtgtggg tacttatcg gaaggtcac ctggagttc 
      accaacccc aacctggaa cgcagcaaa ttgatgcgg 
      aagagtttc catggtagt gctggtaag attctgacc 
      gcggctaaa gtaccaccc cctccaccg ttacgtacc 
      acctcccaa ctgacgacc aataaggag tattacccc 
      ctctaccac aacctggtc gtgacacga gccgacgtc 
      catcaagag ggcgtggac gaagtcgat tgtgcccct 
      ccttcaaac ctcgacctc cccccttat cccagccca 
      ctaggctca gtgatacac tcacgccaa gctagtgaa 
      gaattcccc ccaccacca cccccactt gacttgtcc 
      gcattggac gaacacttg aaaccccca actccgccc 
      cccacccca ccctcaacc cttctagct gagctgcaa 
      gtcaaacga caggagatc ctagcacct ggtgacacc 
      tggctgaag gaactccaa gccaaacaa gccgcctta 
      agaaagaaa caagagagt gtgggttcc cccgggacg 
      tcagtccgc gacttggcc tcaaaattc gaaaacatc 
      caggtgttt gagggccaa aatggcgtc gtcacagcc 
      cggaaacgc gacgatttt gacatcccg cccgaaatt 
      cccgacgaa aaacgcggg aaaaagaaa agtgtctcg 
      ttctgcgac caagtgatc ctagttgcc acagccgag 
      gagcaggaa gacgacagt tacatcccg aacccgatc 
      ctggaacgc gtcctgcgc tcagccatg aacaagccg 
      gaaacggcc gccattcgc caggaagtc cggacttta 
      cgccaggag atgtccccg gagccgttc gtgcgtcgc 
      aactcaatg gacaacctc ctctcgcgg caagccccg 
      ccccctgac cccctctac tacaacaac tacgcccag 
      tcgccctac caacgaatc ccgcagaac tcgccagtt 
      tacaacgcc ccgccctcc tacccccag taccagtat 
      gtccggccg ccggtgtat caacatccg ccgtcgcca 
      cgccccgtc tatcaagag cgcgtcccg ccgctccac 
      cccgagtac acgccctac caaccaatc ccgcgacag 
      ttgctaagg aaaagtgtg agttttgag ccggggacg 
      aaaggcggg gcggagtcg cccactccc aaggcggtg 
      gtcacgccc attgtggtc aataacgcc aataatagc 
      gtgcccacg gggaagacc aagtgtaat ctgtgtagg 
      aagaaggcg gtggtctcg gccaacttg tactgcacg 
      gattgcgag ttttacatg tcgaggttt aagccgaaa 
      aggtagttt ttaaggaag tgtgaagtg taatttatt 
      tggatctca ctgtacata ggattgatt attgtaatg 
      ttttatatt acgtgatta taataaaag agtttttca 
      aat
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