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Gene information
General information
TC011082 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 815762..825208
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011082
    iBeetle screen iB_01800

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011082 RA
      MPETSLTNG YTNGHNNMF EMEDGSNFL FTSESVGEG 
      HPDKMCDQI SDAILDAHL QQDPDAKVA CETVTKTGM 
      ILLCGEITS KAVVDYQKV VRETVAHIG YDDSSKGLD 
      WRTLNLLVA IEQQSPNIA QGVYENRQE PDIGAGFDY 
      KTCNVLLAL DQQSPNIAA GVHENRNEE EVGAGDQGL 
      MFGYATDET EECMPLTVV LAHRLNHQI AELRRNGQF 
      WWARPDSKT QVTCEYTFD HGACIPQRV HTVVVSVQH 
      SEKITLEEL REEILNKVI KTVIPPQYL DDQTIVHIN 
      PCGLFIIGG PQSDAGLTG RKIIVDTYG GWGAHGGGA 
      FSGKDFTKV DRSAAYAAR WVAKSLVKA GLCRRCLVQ 
      VAYAIGVAE PLSITVFDY GTSKLSQKE LLAVVRRNF 
      DLRPGKIVK ELNLRQPIY QRTSTYGHF GRDGFSWEK 
      PKVLRLD
    CDS sequence
    • >TC011082 RA
      atgccggaa acgtcgctc accaacgga tataccaac 
      ggccacaac aacatgttt gaaatggaa gacggctcc 
      aatttcctc ttcacctcc gagtcggtg ggagaagga 
      catccagac aaaatgtgc gaccaaatc agtgatgct 
      attcttgac gcccacctc cagcaagat cctgacgca 
      aaagtggct tgcgaaact gtgacaaaa accggaatg 
      attctcctc tgcggcgaa atcacatca aaggccgtg 
      gtggattac cagaaagtt gtccgagag acagttgca 
      cacattggt tatgacgat tccagtaaa ggattggac 
      tggcgtacg ctgaacctt ctggtggca attgaacag 
      cagtcccca aacatagca cagggtgtt tacgagaac 
      cgtcaagag cccgatatc ggtgctggt tttgattac 
      aagacgtgc aatgtgttg ttggctctc gatcagcag 
      tccccaaac attgctgcc ggcgtgcac gaaaatcga 
      aacgaggag gaagttggc gctggtgac cagggcttg 
      atgttcggc tacgccact gacgagacg gaagagtgt 
      atgccactg acggtggta ctagcccat agacttaac 
      caccaaatc gctgaattg cgccgaaat ggtcaattc 
      tggtgggca agacccgac tcaaaaacg caagtcacc 
      tgcgaatat accttcgac catggcgca tgtattccg 
      caaagggtg cacacggtc gtggtttcg gtgcaacat 
      agcgaaaaa atcacgttg gaagagttg agagaagag 
      attttgaat aaagttata aagactgtg attccccca 
      caatatctc gacgaccaa actatcgta cacatcaac 
      ccgtgcgga ctcttcatc atcggggga ccccagagc 
      gatgccggt ttaaccggt cgcaaaatc atcgtcgat 
      acgtatgga ggctggggg gcgcatgga ggtggcgct 
      ttttcgggt aaagatttc acaaaagtg gatcgttca 
      gcagcgtat gctgctcgt tgggtggcc aaatcgctc 
      gttaaagcc ggactttgc cgaagatgt ttggtgcag 
      gtggcgtat gctatcggc gtagctgaa cctttgtca 
      atcactgtt ttcgactat ggaacctcg aagctttca 
      cagaaggaa ctgcttgcc gtcgtgagg aggaacttc 
      gatctacgg ccggggaaa atcgtcaag gagttgaac 
      cttcgccag cccatctac cagcgtacc agcacctat 
      gggcacttc ggtcgcgat gggttcagt tgggagaag 
      cccaaggtt cttcgtctc gattga
    mRNA sequence
    • >TC011082 RA
      gcaagtcag tctccagcc aagtgctat agtgtgttt 
      tgtttttcg tagatttgg cgccgttcc tcgtccaat 
      cgtcggccg gcccgtccc ccacgtgac ctgcgtctt 
      gttatataa gtggtagac tcccaccat ttcgtcctc 
      ttttccacc aaaagtttt aaaggttgt gtcagtgtt 
      cgaccaagg ttcagaaaa tgccggaaa cgtcgctca 
      ccaacggat ataccaacg gccacaaca acatgtttg 
      aaatggaag acggctcca atttcctct tcacctccg 
      agtcggtgg gagaaggac atccagaca aaatgtgcg 
      accaaatca gtgatgcta ttcttgacg cccacctcc 
      agcaagatc ctgacgcaa aagtggctt gcgaaactg 
      tgacaaaaa ccggaatga ttctcctct gcggcgaaa 
      tcacatcaa aggccgtgg tggattacc agaaagttg 
      tccgagaga cagttgcac acattggtt atgacgatt 
      ccagtaaag gattggact ggcgtacgc tgaaccttc 
      tggtggcaa ttgaacagc agtccccaa acatagcac 
      agggtgttt acgagaacc gtcaagagc ccgatatcg 
      gtgctggtt ttgattaca agacgtgca atgtgttgt 
      tggctctcg atcagcagt ccccaaaca ttgctgccg 
      gcgtgcacg aaaatcgaa acgaggagg aagttggcg 
      ctggtgacc agggcttga tgttcggct acgccactg 
      acgagacgg aagagtgta tgccactga cggtggtac 
      tagcccata gacttaacc accaaatcg ctgaattgc 
      gccgaaatg gtcaattct ggtgggcaa gacccgact 
      caaaaacgc aagtcacct gcgaatata ccttcgacc 
      atggcgcat gtattccgc aaagggtgc acacggtcg 
      tggtttcgg tgcaacata gcgaaaaaa tcacgttgg 
      aagagttga gagaagaga ttttgaata aagttataa 
      agactgtga ttcccccac aatatctcg acgaccaaa 
      ctatcgtac acatcaacc cgtgcggac tcttcatca 
      tcgggggac cccagagcg atgccggtt taaccggtc 
      gcaaaatca tcgtcgata cgtatggag gctgggggg 
      cgcatggag gtggcgctt tttcgggta aagatttca 
      caaaagtgg atcgttcag cagcgtatg ctgctcgtt 
      gggtggcca aatcgctcg ttaaagccg gactttgcc 
      gaagatgtt tggtgcagg tggcgtatg ctatcggcg 
      tagctgaac ctttgtcaa tcactgttt tcgactatg 
      gaacctcga agctttcac agaaggaac tgcttgccg 
      tcgtgagga ggaacttcg atctacggc cggggaaaa 
      tcgtcaagg agttgaacc ttcgccagc ccatctacc 
      agcgtacca gcacctatg ggcacttcg gtcgcgatg 
      ggttcagtt gggagaagc ccaaggttc ttcgtctcg 
      attgacctt cccccctca gttaatttc cttccttgc 
      cccttcgtc cctcgtctt ctctgtcgt agctctcct 
      taattaaaa catgtcgtt cgagcgggt gctattgga 
      cgtggtacc ggcctgtac agatcacag tagttacca 
      tcatttttt tcgagttgg tactactgc aactctgtg 
      tagagtgaa aggttctgt tcccgctag acttgtttt 
      cactcgaca ccttgtgtt gtgtttacg aaaaagaat 
      ctatttgga aaagagcat ttgcgaaaa cactccgat 
      ctgtgcaag tccggttct cgggtgtga ttggttaaa 
      taaaacaaa tgacacatg atttcggca tttatcaag 
      ctgccacta aaattctgt tctctttta tagtgttct 
      tattgttat aatgactta tactgacca taggtgtag 
      atctgtagt gataggata ctgtataga ttttaaacg 
      tgaatattt aaattattt atatggcgt gtaatatga 
      aaagtcagt cgatggtgt actttattt aagtgctat 
      ttcaaggag ctttcgtca ttattttaa acaataaag 
      tgattatta ggaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY