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Gene information
General information
TC011081 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 833144..837448
Sequence information
Transcripts / Proteins (4) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011081
    iBeetle screen iB_01799

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011081 RA
      MGSSRHRDR SRDRDRRDR DRDRDRDRR DRRKSSRDR 
      HRDRDRDRE RRDRDRDRS RSREKRREK TPEPDGEKL 
      IADTLATIA ATRNFAALI APKPEPDTN SNQSNGSQE 
      EEGSTRKRK RRSRWAGGE HDKIFIPGM PTILPSGLD 
      KNQEQAYLL QLQIEEVSR KLRSGDLGI PPNPEDRSP 
      SPEPIYSSD GKRLNTREF RTRKKLEEE RHSLILKMQ 
      QINPDFKPP MDYNCRPPV VRVSDKVMI PQEEHPEIN 
      FVGLLIGPR GNTLKTMEK ETGAKIIIR GKGSVKEGK 
      VGRKDGQPL PGEDEPLHA YITATNPEC VKKAVERIK 
      EVIRQGVEV PENQNDLRR MQLRELAQL NGTLRENDG 
      MRCNNCGAT DHKSWLCPD KPNVTNNIV CSSCGAAGH 
      IARDCRQKR PGAGGPPVP GGEKNKIDE EYMSLMAEL 
      GEAPPPEAV ATTTTTTTT RKSSFNLFD SPMTPRPLM 
      PPPMPTPTA LQCMNGMAP PPWTVAAQN MSWQPGPPG 
      IMPPPPPPG ASSPPQMMQ WMGANNQPP PPGASTTNG 
      TMPWQAAAF TWPPGSCPP PPPPGIDVN SLLTTPPPP 
      PPS
    • >TC011081 RB
      MIPQEEHPE INFVGLLIG PRGNTLKTM EKETGAKII 
      IRGKGSVKE GKVGRKDGQ PLPGEDEPL HAYITATNP 
      ECVKKAVER IKEVIRQGV EVPENQNDL RRMQLRELA 
      QLNGTLREN DGMRCNNCG ATDHKSWLC PDKPNVTNN 
      IVCSSCGAA GHIARDCRQ KRPGAGGPP VPGGEKNKI 
      DEEYMSLMA ELGEAPPPE AVATTTTTT TTRKSSFNL 
      FDSPMTPRP LMPPPMPTP TALQCMNGM APPPWTVAA 
      QNMSWQPGP PGIMPPPPP PGASSPPQM MQWMGANNQ 
      PPPPGASTT NGTMPWQAA AFTWPPGSC PPPPPPGID 
      VNSLLTTPP PPPPS
    • >TC011081 RC
      MGSSRHRDR SRDRDRRDR DRDRDRDRR DRRKSSRDR 
      HRDRDRDRE RRDRDRDRS RSREKRREK TPEPDGEKL 
      IADTLATIA ATRNFAALI APKPEPDTN SNQSNGSQE 
      EEGSTRKRK RRSRWAGGE HDKIFIPGM PTILPSGLD 
      KNQEQAYLL QLQIEEVSR KLRSGDLGI PPNPEDR
    • >TC011081 RD
      MGSSRHRDR SRDRDRRDR DRDRDRDRR DRRKSSRDR 
      HRDRDRDRE RRDRDRDRS RSREKRREK TPEPDGEKL 
      IADTLATIA ATRNFAALI APKPEPDTN SNQSNGSQE 
      EEGSTRKRK RRSRWAGGE HDKIFIPGM PTILPSGLD 
      KNQEQAYLR KYQIFSLVC
    CDS sequence
    • >TC011081 RA
      atggggtct tcgagacac cgcgaccgg agccgggac 
      cgggataga agagaccgg gaccgggac agggatcgg 
      gatcgccgg gacaggcgg aagagcagt cgagacagg 
      catagggac agggacagg gatagggag cgcagggat 
      cgggacagg gaccggagt cgcagtcgc gagaaacga 
      agggagaag actccggag cctgatggc gagaaattg 
      atcgccgac acgttggcg actattgca gccacgagg 
      aatttcgcg gctttgatc gcccccaag ccggagccc 
      gataccaat tcgaatcag tcgaacggg agccaagag 
      gaggagggc agcacccga aaacgcaaa aggcgctcc 
      cgatgggct ggaggcgaa cacgacaaa attttcatc 
      cctgggatg cccacaatc ctcccctcc ggcttggac 
      aagaaccag gagcaggca tatttgctt caactccag 
      attgaggaa gtcagtaga aaactgcgc tccggagac 
      ctggggatt ccaccaaat ccagaggac agatcacca 
      tcacctgaa ccgatctac agcagcgat ggcaaacgt 
      ctaaacacg cgcgaattt cgcaccaga aaaaaactg 
      gaggaagaa agacattcg ctcattttg aaaatgcaa 
      cagatcaac ccagatttc aaaccgcct atggattac 
      aattgtaga ccccctgtg gttcgtgta agtgataaa 
      gttatgata ccgcaagag gaacatcct gagattaat 
      tttgtaggt ttgttgatt ggtccaagg gggaatacg 
      ttaaaaaca atggagaaa gaaactggg gctaaaatt 
      attatcagg ggtaaggga agtgtcaaa gagggtaaa 
      gttggacga aaagacgga caaccactt cccggtgag 
      gatgaaccc ttgcatgcc tacatcacg gccacgaat 
      cctgaatgc gtcaagaag gccgtggaa cggatcaag 
      gaggttata cgtcaggga gtggaagtc cccgaaaac 
      caaaacgac cttcgccga atgcaattg cgtgagttg 
      gcacaactg aacggcaca ttgcgcgaa aatgacgga 
      atgcgttgt aacaactgt ggcgccact gaccacaaa 
      tcttggctg tgccccgat aaaccaaac gtcaccaac 
      aacattgtc tgttcgagt tgtggggct gccggccat 
      atcgcacgc gactgtcgt cagaagcgt cccggcgcc 
      gggggcccc cctgtcccc ggcggcgaa aagaacaaa 
      atcgacgag gagtacatg tcgttgatg gcggagttg 
      ggtgaggcg ccgcccccg gaggcggtc gctaccacc 
      accaccacg acgacgacg cgtaaatcg agttttaat 
      ttgtttgat tcgccgatg acgccacgc ccattgatg 
      ccgcctccg atgccgacg cctactgcg cttcagtgt 
      atgaatggt atggcgccg cctccgtgg acggtagcg 
      gcacagaat atgagttgg cagccggga ccgccgggc 
      attatgccg ccgccaccg ccgccagga gcctccagt 
      ccgccacag atgatgcaa tggatgggg gcgaataat 
      cagccgccg ccgcctggg gcaagtacg acgaatggg 
      actatgccg tggcaagcg gcggcgttc acgtggccg 
      ccggggagt tgtccgcca ccgccgcca ccagggatt 
      gatgttaat agcttgttg acgacgccg ccgccaccg 
      ccgccgtcg taa
    • >TC011081 RB
      atgataccg caagaggaa catcctgag attaatttt 
      gtaggtttg ttgattggt ccaaggggg aatacgtta 
      aaaacaatg gagaaagaa actggggct aaaattatt 
      atcaggggt aagggaagt gtcaaagag ggtaaagtt 
      ggacgaaaa gacggacaa ccacttccc ggtgaggat 
      gaacccttg catgcctac atcacggcc acgaatcct 
      gaatgcgtc aagaaggcc gtggaacgg atcaaggag 
      gttatacgt cagggagtg gaagtcccc gaaaaccaa 
      aacgacctt cgccgaatg caattgcgt gagttggca 
      caactgaac ggcacattg cgcgaaaat gacggaatg 
      cgttgtaac aactgtggc gccactgac cacaaatct 
      tggctgtgc cccgataaa ccaaacgtc accaacaac 
      attgtctgt tcgagttgt ggggctgcc ggccatatc 
      gcacgcgac tgtcgtcag aagcgtccc ggcgccggg 
      ggcccccct gtccccggc ggcgaaaag aacaaaatc 
      gacgaggag tacatgtcg ttgatggcg gagttgggt 
      gaggcgccg cccccggag gcggtcgct accaccacc 
      accacgacg acgacgcgt aaatcgagt tttaatttg 
      tttgattcg ccgatgacg ccacgccca ttgatgccg 
      cctccgatg ccgacgcct actgcgctt cagtgtatg 
      aatggtatg gcgccgcct ccgtggacg gtagcggca 
      cagaatatg agttggcag ccgggaccg ccgggcatt 
      atgccgccg ccaccgccg ccaggagcc tccagtccg 
      ccacagatg atgcaatgg atgggggcg aataatcag 
      ccgccgccg cctggggca agtacgacg aatgggact 
      atgccgtgg caagcggcg gcgttcacg tggccgccg 
      gggagttgt ccgccaccg ccgccacca gggattgat 
      gttaatagc ttgttgacg acgccgccg ccaccgccg 
      ccgtcgtaa
    • >TC011081 RC
      atggggtct tcgagacac cgcgaccgg agccgggac 
      cgggataga agagaccgg gaccgggac agggatcgg 
      gatcgccgg gacaggcgg aagagcagt cgagacagg 
      catagggac agggacagg gatagggag cgcagggat 
      cgggacagg gaccggagt cgcagtcgc gagaaacga 
      agggagaag actccggag cctgatggc gagaaattg 
      atcgccgac acgttggcg actattgca gccacgagg 
      aatttcgcg gctttgatc gcccccaag ccggagccc 
      gataccaat tcgaatcag tcgaacggg agccaagag 
      gaggagggc agcacccga aaacgcaaa aggcgctcc 
      cgatgggct ggaggcgaa cacgacaaa attttcatc 
      cctgggatg cccacaatc ctcccctcc ggcttggac 
      aagaaccag gagcaggca tatttgctt caactccag 
      attgaggaa gtcagtaga aaactgcgc tccggagac 
      ctggggatt ccaccaaat ccagaggac aggtga
    • >TC011081 RD
      atggggtct tcgagacac cgcgaccgg agccgggac 
      cgggataga agagaccgg gaccgggac agggatcgg 
      gatcgccgg gacaggcgg aagagcagt cgagacagg 
      catagggac agggacagg gatagggag cgcagggat 
      cgggacagg gaccggagt cgcagtcgc gagaaacga 
      agggagaag actccggag cctgatggc gagaaattg 
      atcgccgac acgttggcg actattgca gccacgagg 
      aatttcgcg gctttgatc gcccccaag ccggagccc 
      gataccaat tcgaatcag tcgaacggg agccaagag 
      gaggagggc agcacccga aaacgcaaa aggcgctcc 
      cgatgggct ggaggcgaa cacgacaaa attttcatc 
      cctgggatg cccacaatc ctcccctcc ggcttggac 
      aagaaccag gagcaggca tatttgcgt aagtaccaa 
      attttctca ctagtttgt taa
    mRNA sequence
    • >TC011081 RA
      cgcctggcg gcggccatt ttccgctcc atcttgtcg 
      tgttgttct tgctcttgc tgtaatttg tgtttgtgt 
      aaaatgggg tcttcgaga caccgcgac cggagccgg 
      gaccgggat agaagagac cgggaccgg gacagggat 
      cgggatcgc cgggacagg cggaagagc agtcgagac 
      aggcatagg gacagggac agggatagg gagcgcagg 
      gatcgggac agggaccgg agtcgcagt cgcgagaaa 
      cgaagggag aagactccg gagcctgat ggcgagaaa 
      ttgatcgcc gacacgttg gcgactatt gcagccacg 
      aggaatttc gcggctttg atcgccccc aagccggag 
      cccgatacc aattcgaat cagtcgaac gggagccaa 
      gaggaggag ggcagcacc cgaaaacgc aaaaggcgc 
      tcccgatgg gctggaggc gaacacgac aaaattttc 
      atccctggg atgcccaca atcctcccc tccggcttg 
      gacaagaac caggagcag gcatatttg cttcaactc 
      cagattgag gaagtcagt agaaaactg cgctccgga 
      gacctgggg attccacca aatccagag gacagatca 
      ccatcacct gaaccgatc tacagcagc gatggcaaa 
      cgtctaaac acgcgcgaa tttcgcacc agaaaaaaa 
      ctggaggaa gaaagacat tcgctcatt ttgaaaatg 
      caacagatc aacccagat ttcaaaccg cctatggat 
      tacaattgt agaccccct gtggttcgt gtaagtgat 
      aaagttatg ataccgcaa gaggaacat cctgagatt 
      aattttgta ggtttgttg attggtcca agggggaat 
      acgttaaaa acaatggag aaagaaact ggggctaaa 
      attattatc aggggtaag ggaagtgtc aaagagggt 
      aaagttgga cgaaaagac ggacaacca cttcccggt 
      gaggatgaa cccttgcat gcctacatc acggccacg 
      aatcctgaa tgcgtcaag aaggccgtg gaacggatc 
      aaggaggtt atacgtcag ggagtggaa gtccccgaa 
      aaccaaaac gaccttcgc cgaatgcaa ttgcgtgag 
      ttggcacaa ctgaacggc acattgcgc gaaaatgac 
      ggaatgcgt tgtaacaac tgtggcgcc actgaccac 
      aaatcttgg ctgtgcccc gataaacca aacgtcacc 
      aacaacatt gtctgttcg agttgtggg gctgccggc 
      catatcgca cgcgactgt cgtcagaag cgtcccggc 
      gccgggggc ccccctgtc cccggcggc gaaaagaac 
      aaaatcgac gaggagtac atgtcgttg atggcggag 
      ttgggtgag gcgccgccc ccggaggcg gtcgctacc 
      accaccacc acgacgacg acgcgtaaa tcgagtttt 
      aatttgttt gattcgccg atgacgcca cgcccattg 
      atgccgcct ccgatgccg acgcctact gcgcttcag 
      tgtatgaat ggtatggcg ccgcctccg tggacggta 
      gcggcacag aatatgagt tggcagccg ggaccgccg 
      ggcattatg ccgccgcca ccgccgcca ggagcctcc 
      agtccgcca cagatgatg caatggatg ggggcgaat 
      aatcagccg ccgccgcct ggggcaagt acgacgaat 
      gggactatg ccgtggcaa gcggcggcg ttcacgtgg 
      ccgccgggg agttgtccg ccaccgccg ccaccaggg 
      attgatgtt aatagcttg ttgacgacg ccgccgcca 
      ccgccgccg tcgtaagag atatgaaga ttgcacgtg 
      gtatttacc gtagggtag ttaaataat tatgggatt 
      tcattattg tatttaata ttggtatca attattaat 
      tcttcaata taatgtgta ttatgga
    • >TC011081 RB
      tgtccgcta atttaataa ttaattaat taattaaca 
      ggagtatca tccactgaa attgacagc agttggtac 
      aacgtcaca caatataat ctaatttac tttcagcca 
      tcaagaagg cgataatta atttttact attacagca 
      attaattaa tcaattctt tctttagga aaagcgaat 
      aaggaaatt gtttttggg ggcaatttt aagccgata 
      aaacagttt tcttctttc atacaattt gaaaaattt 
      acttattca cttaaattg aacagttta ggtttaaga 
      gaggtcgct ttgcacatc gcttgaaat tttcaattt 
      tttttgtaa tatactata ccatataca ggggtattt 
      gaaaatttc aagcggtga ggcgaagcc gagtacttc 
      tctctcatt aattcattt tttcatcta tgtgtgcag 
      atcaccatc acctgaacc gatctacag cagcgatgg 
      caaacgtct aaacacgcg cgaatttcg caccagaaa 
      aaaactgga ggaagaaag acattcgct cattttgaa 
      aatgcaaca gatcaaccc agatttcaa accgcctat 
      ggattacaa ttgtagacc ccctgtggt tcgtgtaag 
      tgataaagt tatgatacc gcaagagga acatcctga 
      gattaattt tgtaggttt gttgattgg tccaagggg 
      gaatacgtt aaaaacaat ggagaaaga aactggggc 
      taaaattat tatcagggg taagggaag tgtcaaaga 
      gggtaaagt tggacgaaa agacggaca accacttcc 
      cggtgagga tgaaccctt gcatgccta catcacggc 
      cacgaatcc tgaatgcgt caagaaggc cgtggaacg 
      gatcaagga ggttatacg tcagggagt ggaagtccc 
      cgaaaacca aaacgacct tcgccgaat gcaattgcg 
      tgagttggc acaactgaa cggcacatt gcgcgaaaa 
      tgacggaat gcgttgtaa caactgtgg cgccactga 
      ccacaaatc ttggctgtg ccccgataa accaaacgt 
      caccaacaa cattgtctg ttcgagttg tggggctgc 
      cggccatat cgcacgcga ctgtcgtca gaagcgtcc 
      cggcgccgg gggcccccc tgtccccgg cggcgaaaa 
      gaacaaaat cgacgagga gtacatgtc gttgatggc 
      ggagttggg tgaggcgcc gcccccgga ggcggtcgc 
      taccaccac caccacgac gacgacgcg taaatcgag 
      ttttaattt gtttgattc gccgatgac gccacgccc 
      attgatgcc gcctccgat gccgacgcc tactgcgct 
      tcagtgtat gaatggtat ggcgccgcc tccgtggac 
      ggtagcggc acagaatat gagttggca gccgggacc 
      gccgggcat tatgccgcc gccaccgcc gccaggagc 
      ctccagtcc gccacagat gatgcaatg gatgggggc 
      gaataatca gccgccgcc gcctggggc aagtacgac 
      gaatgggac tatgccgtg gcaagcggc ggcgttcac 
      gtggccgcc ggggagttg tccgccacc gccgccacc 
      agggattga tgttaatag cttgttgac gacgccgcc 
      gccaccgcc gccgtcgta agagatatg aagattgca 
      cgtggtatt taccgtagg gtagttaaa taattatgg 
      gatttcatt attgtattt aatattggt atcaattat 
      taattcttc aatataatg tgtattatg ga
    • >TC011081 RC
      cgcctggcg gcggccatt ttccgctcc atcttgtcg 
      tgttgttct tgctcttgc tgtaatttg tgtttgtgt 
      aaaatgggg tcttcgaga caccgcgac cggagccgg 
      gaccgggat agaagagac cgggaccgg gacagggat 
      cgggatcgc cgggacagg cggaagagc agtcgagac 
      aggcatagg gacagggac agggatagg gagcgcagg 
      gatcgggac agggaccgg agtcgcagt cgcgagaaa 
      cgaagggag aagactccg gagcctgat ggcgagaaa 
      ttgatcgcc gacacgttg gcgactatt gcagccacg 
      aggaatttc gcggctttg atcgccccc aagccggag 
      cccgatacc aattcgaat cagtcgaac gggagccaa 
      gaggaggag ggcagcacc cgaaaacgc aaaaggcgc 
      tcccgatgg gctggaggc gaacacgac aaaattttc 
      atccctggg atgcccaca atcctcccc tccggcttg 
      gacaagaac caggagcag gcatatttg cttcaactc 
      cagattgag gaagtcagt agaaaactg cgctccgga 
      gacctgggg attccacca aatccagag gacaggtga 
      agtgttact accaacatt attttcccg accatcaga 
      ccatcggca gcggtttgg caacgatta ccaatcaat 
      aatgttttt agttcattt tttacttta atcgattaa 
      aatttgcac caaattttg aacagatgg ccgaggcac 
      agggggttc cccttctgt tttttcttt aaccaaatc 
      ctgcgattt tttttatct tgttcttaa ttgaatacc 
      taatcgcgt tttttgccg aaaatctgt tgattgttc 
      ggtttgttt tagtgaaat tttgaaaat gg
    • >TC011081 RD
      cgcctggcg gcggccatt ttccgctcc atcttgtcg 
      tgttgttct tgctcttgc tgtaatttg tgtttgtgt 
      aaaatgggg tcttcgaga caccgcgac cggagccgg 
      gaccgggat agaagagac cgggaccgg gacagggat 
      cgggatcgc cgggacagg cggaagagc agtcgagac 
      aggcatagg gacagggac agggatagg gagcgcagg 
      gatcgggac agggaccgg agtcgcagt cgcgagaaa 
      cgaagggag aagactccg gagcctgat ggcgagaaa 
      ttgatcgcc gacacgttg gcgactatt gcagccacg 
      aggaatttc gcggctttg atcgccccc aagccggag 
      cccgatacc aattcgaat cagtcgaac gggagccaa 
      gaggaggag ggcagcacc cgaaaacgc aaaaggcgc 
      tcccgatgg gctggaggc gaacacgac aaaattttc 
      atccctggg atgcccaca atcctcccc tccggcttg 
      gacaagaac caggagcag gcatatttg cgtaagtac 
      caaattttc tcactagtt tgttaatta agtaggttt 
      gttgtatgt gtgtaaagc tggagccaa gataatatg 
      agaaaaaat tttttagat cgtatttca cttttgttg 
      aacaaaaat gctaactct tgggctttt gccctcctc 
      taacccgtt tcattacag ttcaactcc agattgagg 
      aagtcagta gaaaactgc gctccggag acctgggga 
      ttccaccaa atccagagg acaggtgaa gtgttacta 
      ccaacatta ttttcccga ccatcagac catcggcag 
      cggtttggc aacgattac caatcaata atgttttta 
      gttcatttt ttactttaa tcgattaaa atttgcacc 
      aaattttga acagatggc cgaggcaca gggggttcc 
      ccttctgtt ttttcttta accaaatcc tgcgatttt 
      ttttatctt gttcttaat tgaatacct aatcgcgtt 
      ttttgccga aaatctgtt gattgttcg gtttgtttt 
      agtgaaatt ttgaaaatg g
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