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Gene information
General information
TC011075 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5738170..5739324
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011075
    iBeetle screen iB_01798

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    oblique dorsal thoracic muscles median not present - Number of animals showing the phenotype: 9

    Adult morphology
    abdominal odoriferous glands sometimes darker - Number of animals showing the phenotype: 5 (41 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011075 RA
      MASAVTRVV HRCEDAQES QNLNLSNCQ 
      LMQVPDAVY HLMRHTELK TCDLSDNVI 
      TKIPPKFAV KFSSITDLN LSHNQMAKL 
      PDEIADLGA LERLDISHN TFISLPDCI 
      FKISKLQQL NASNNHIID FDVECMERA 
      PSLETVDLS NNPLSPRCH DQCSAISRI 
      RITLTPREK EDWEDLTV
    CDS sequence
    • >TC011075 RA
      atggcctcg gcggtgacc cgcgtcgtc caccggtgc 
      gaagacgca caagaatcg caaaacctg aatctctca 
      aattgccaa ttgatgcaa gtccccgac gccgtctac 
      cacctcatg cgtcacacc gaactgaaa acctgtgat 
      ttaagtgat aatgtgatt acgaaaata cctccgaaa 
      ttcgccgtt aagttcagc tcgataacg gacttgaac 
      ttgtcgcac aatcaaatg gcgaagctt ccggacgaa 
      atcgcagac ttgggcgcg ctggagcgg ctggatatc 
      tcacacaat acctttatt tccttgcca gattgtata 
      ttcaaaatc agcaaactg cagcaactc aacgccagt 
      aataaccac ataattgat ttcgatgtg gagtgcatg 
      gagagagcg ccctcgtta gaaacggta gacttaagc 
      aacaacccc ttgtctccg cgatgccac gaccagtgt 
      tcggcgata tccaggatc aggataacg ctgacgccg 
      agagagaaa gaggactgg gaagatctc accgtttag
    mRNA sequence
    • >TC011075 RA
      tatgcgatg attcacttt cgaaatggc ctcggcggt 
      gacccgcgt cgtccaccg gtgcgaaga cgcacaaga 
      atcgcaaaa cctgaatct ctcaaattg ccaattgat 
      gcaagtccc cgacgccgt ctaccacct catgcgtca 
      caccgaact gaaaacctg tgatttaag tgataatgt 
      gattacgaa aatacctcc gaaattcgc cgttaagtt 
      cagctcgat aacggactt gaacttgtc gcacaatca 
      aatggcgaa gcttccgga cgaaatcgc agacttggg 
      cgcgctgga gcggctgga tatctcaca caatacctt 
      tatttcctt gccagattg tatattcaa aatcagcaa 
      actgcagca actcaacgc cagtaataa ccacataat 
      tgatttcga tgtggagtg catggagag agcgccctc 
      gttagaaac ggtagactt aagcaacaa ccccttgtc 
      tccgcgatg ccacgacca gtgttcggc gatatccag 
      gatcaggat aacgctgac gccgagaga gaaagagga 
      ctgggaaga tctcaccgt ttagccaat gacaatcgt 
      ggcttttca ctgcctttg ctaatcaaa aactgtttc 
      aatgccagg ttgggacac gtgcaataa tctcccttt 
      tgagacatt tttgattac ttctggtag ctcaatccc 
      aaagcaggg tgctaaacc taaggcctg ttagtctaa 
      tttaaataa agtatttgt attttttat aattgatat 
      tgtagttga aacgtacta atgacgaga cggtgagtc 
      gtgatcttt tgttttatt tctttcgtt taaggtcgg 
      attcacggt ttcgcagat gtgtgtatt attttgtac 
      tgacactgt gttgtttat attattgta tatttataa 
      ttaattcaa gttgatttt gtacttatt tgacagagt 
      ttcggcgat ttcatctag tcatgttgt gatagggac 
      gcgttggcg tctttgtag tgtctagat gtgtccaaa 
      tgatggaaa aaagtgtca aataaatga acaaacacg 
      aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY