Status
Please wait ...
Gene information
General information
TC034906 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5695127..5697481
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034906
    iBeetle screen iB_01797

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034906 RA
      MHQQDLEKL YSPSQWTRR FSSADEVIQ HHLKFIEKA 
      SAETRDKVP SQLDVPYGS TQRQKIDIF GTDLDDGAP 
      IFVFVHGGY WQMKAISKS SYHFLARNF YKNGIKSIF 
      IGYDLCPDV PLSQIVAQT QSAVDKCLE YAKETRSKG 
      LYLMGHSAG AHLVASSLH SVPPQDRKL VKGAILASG 
      VFDLRPLVS TEVNQPLKL DEKLATELS PLKQDFAKE 
      FGETSFLVI VGEYESPAF VKQSELFSE KLLNLGLKS 
      QFWVVPKVD HFDIIESFF EDSFTIFSK ISAYFHSKT 
      SNMTDVEKL FLPNQWSKR FAPDVIVDK HLEFAAKGS 
      ETARQKVPS QLNVKYGNL EKEKIDIFG TDLPNEAPI 
      VGFVHGGYW QKEYLNRST YHFLAPILH KNGIKSMFV 
      GYELCPKVT LDQIITEIT LATKQCLKY AHENGSKGF 
      YLAGYSAGA HLVAALYTN LAPISLSNE ERNMIKSVF 
      LIAGIYDLR ELPKTCVNE PLKLSQSEA EKLSPLLQN 
      VVKGVTTKF HVISARNDS PEFQKQAKM FDEKLRNGG 
      VVTKWTNVE DVDHFDVVE RLVDENFEV TKLILVQIH 
      NPDE
    CDS sequence
    • >TC034906 RA
      atgcaccaa caagactta gagaaattg tattcaccg 
      agtcagtgg actcgaagg ttttcttcc gctgacgaa 
      gtcatccag catcattta aaatttata gaaaaagct 
      agtgctgaa acacgtgat aaagtccca tcgcaactt 
      gatgtccca tatgggagc acccaacgc caaaaaatc 
      gacattttt gggacggat ttggacgac ggggcccca 
      atttttgtt tttgtgcac ggaggctac tggcaaatg 
      aaggccatc tccaagtcc agttatcac tttctggcc 
      cggaacttt tacaaaaac ggtatcaag agcattttt 
      attggctat gacctttgc cccgacgtc cccttatca 
      caaatagtg gcccaaacc caatcagct gttgacaaa 
      tgcctcgaa tatgcgaaa gaaacacgc tcaaaaggc 
      ttgtatttg atggggcac tcggcgggg gcccatctc 
      gtggcctca agcctccac tctgtgccc ccacaagac 
      cgcaagctg gtcaagggg gccattctc gcgtcggga 
      gtcttcgat ttgaggcca cttgtctca actgaagtt 
      aaccaaccg ttgaaactt gacgaaaaa ctggccaca 
      gaattgagt cctttaaaa caagatttt gcgaaagaa 
      tttggcgaa acgtcgttt cttgtgatt gtgggggaa 
      tacgagagt ccagcattt gtgaaacaa tcggaacta 
      tttagcgaa aagttgctc aatttgggc ctcaaatcg 
      cagttttgg gtggtccca aaagtggac catttcgac 
      ataattgag tcgtttttt gaggatagt tttacaatt 
      tttagcaaa atttcggcc tactttcac tccaaaacc 
      tccaacatg acggatgtt gaaaagtta tttttaccc 
      aaccagtgg tctaaaaga tttgcacct gatgtgata 
      gttgacaaa catctagaa tttgcagct aaagggagt 
      gagacagca agacagaaa gtaccgtcc caactcaac 
      gtcaaatat ggaaatttg gaaaaagaa aaaatcgat 
      attttcgga accgattta cccaatgaa gccccaatt 
      gtgggtttc gtccatggg ggctattgg cagaaagag 
      tacttgaac cgttccact taccacttc ctggccccc 
      attttgcac aaaaatggc atcaaatca atgtttgtg 
      ggctacgag ttgtgcccc aaagtcact ctggaccaa 
      attatcact gaaataaca ctcgctact aaacagtgt 
      ctcaaatat gcgcacgaa aatggctcc aagggcttt 
      tacttggcg ggatattca gctggggcc catcttgta 
      gccgcgttg tatacaaac ttggcccca ataagtctc 
      tcaaacgag gagcggaac atgatcaaa tcagttttt 
      ctcattgct ggaatttac gatttgagg gaactaccc 
      aaaacttgt gttaatgaa cccttgaaa ctgagccaa 
      agtgaagct gagaagttg agccccttg ttgcaaaat 
      gtagtgaaa ggagtaaca actaagttt catgtgatt 
      tcggccaga aatgatagt cctgagttt caaaaacag 
      gccaaaatg tttgatgag aaactgcga aatggcggc 
      gtggtcaca aaatggacg aatgtcgaa gatgtggac 
      cattttgat gtggttgaa aggctggtg gatgagaat 
      tttgaggtc accaagttg attttggtg caaatacac 
      aatcccgat gaatag
    mRNA sequence
    • >TC034906 RA
      atgtattta tctttctta tcagccaac cagacaatt 
      accaaacaa ttctttgaa tttagttga attcaatca 
      ataattaac aaagaaggg agtcgaagc tgcaataat 
      aaggtttcc aggtttcca acttgcaac tcgatatct 
      gaactaaat ttcaacttg aactaacaa tgcaccaac 
      aagacttag agaaattgt attcaccga gtcagtgga 
      ctcgaaggt tttcttccg ctgacgaag tcatccagc 
      atcatttaa aatttatag aaaaagcta gtgctgaaa 
      cacgtgata aagtcccat cgcaacttg atgtcccat 
      atgggagca cccaacgcc aaaaaatcg acatttttg 
      ggacggatt tggacgacg gggccccaa tttttgttt 
      ttgtgcacg gaggctact ggcaaatga aggccatct 
      ccaagtcca gttatcact ttctggccc ggaactttt 
      acaaaaacg gtatcaaga gcattttta ttggctatg 
      acctttgcc ccgacgtcc ccttatcac aaatagtgg 
      cccaaaccc aatcagctg ttgacaaat gcctcgaat 
      atgcgaaag aaacacgct caaaaggct tgtatttga 
      tggggcact cggcggggg cccatctcg tggcctcaa 
      gcctccact ctgtgcccc cacaagacc gcaagctgg 
      tcaaggggg ccattctcg cgtcgggag tcttcgatt 
      tgaggccac ttgtctcaa ctgaagtta accaaccgt 
      tgaaacttg acgaaaaac tggccacag aattgagtc 
      ctttaaaac aagattttg cgaaagaat ttggcgaaa 
      cgtcgtttc ttgtgattg tgggggaat acgagagtc 
      cagcatttg tgaaacaat cggaactat ttagcgaaa 
      agttgctca atttgggcc tcaaatcgc agttttggg 
      tggtcccaa aagtggacc atttcgaca taattgagt 
      cgttttttg aggatagtt ttacaattt ttagcaaaa 
      tttcggcct actttcact ccaaaacct ccaacatga 
      cggatgttg aaaagttat ttttaccca accagtggt 
      ctaaaagat ttgcacctg atgtgatag ttgacaaac 
      atctagaat ttgcagcta aagggagtg agacagcaa 
      gacagaaag taccgtccc aactcaacg tcaaatatg 
      gaaatttgg aaaaagaaa aaatcgata ttttcggaa 
      ccgatttac ccaatgaag ccccaattg tgggtttcg 
      tccatgggg gctattggc agaaagagt acttgaacc 
      gttccactt accacttcc tggccccca ttttgcaca 
      aaaatggca tcaaatcaa tgtttgtgg gctacgagt 
      tgtgcccca aagtcactc tggaccaaa ttatcactg 
      aaataacac tcgctacta aacagtgtc tcaaatatg 
      cgcacgaaa atggctcca agggctttt acttggcgg 
      gatattcag ctggggccc atcttgtag ccgcgttgt 
      atacaaact tggccccaa taagtctct caaacgagg 
      agcggaaca tgatcaaat cagtttttc tcattgctg 
      gaatttacg atttgaggg aactaccca aaacttgtg 
      ttaatgaac ccttgaaac tgagccaaa gtgaagctg 
      agaagttga gccccttgt tgcaaaatg tagtgaaag 
      gagtaacaa ctaagtttc atgtgattt cggccagaa 
      atgatagtc ctgagtttc aaaaacagg ccaaaatgt 
      ttgatgaga aactgcgaa atggcggcg tggtcacaa 
      aatggacga atgtcgaag atgtggacc attttgatg 
      tggttgaaa ggctggtgg atgagaatt ttgaggtca 
      ccaagttga ttttggtgc aaatacaca atcccgatg 
      aataggcgt aaaaacgta tattgggtt gcataacca 
      ttttttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY