Status
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Gene information
General information
TC011068 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5605154..5612307
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011068
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01796

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011068 RA
      MAQYLVFFL ALFLFKSAL SQSNYADQA NNIEYQGKG 
      LPDQATLDG KVTKLDDLS PVIFLNRTK AALNCAAGS 
      MQVELKFND KFYGIAYAD FDRNSACQV YGKGGYSYK 
      LELPLKGCG TKQDPQRVF TNNIVVRFH PGLEIDGDE 
      IITIVCRYP PPVAPLPDP LIDFSKEVP PPARVLEPA 
      LKPFQILLI FCGILFLSL LLLGLGCSY YCLRRRNLT 
      VVRKPFSPL TDGSEITKV SGSSLGNLS MFEGLKIPR 
      VSAPVVAAP GSSSGSEGP LISDTLPSD YPSESHSEA 
      EDARSLPPS SGGSFENQA YVADTFYTE PPRLPLAKE 
      PKFDVQVRV KRAPPSPPS LSISDTESS ARNLSTIME 
      QREESVRSI DSPLPVERT HFTYVPELH PPPKHIQPA 
      PTYNRILRR QQELQEERS IASLNTEMT DTHSMTEMI 
      DDSHHKPAP PPPPPIIPR QEYVVDFAP PPTLEPPIA 
      VVHKPEITS HVVDDVFLR TITEKKTIE DIERHKRLI 
      TEYHTRAKA IPEQKWDVT IKNYPVEMP EPPEWENFS 
      DISSASGMT LTPKPERAT MSLPPQSTI DDNKLPLNS 
      PELVGSVTQ QGTFLETFN IPLENPEVP NWSVLIRVL 
      QPEQEEGPV IESAETFNS QLTLSDKMK WRQIITTES 
      TLRTLLTEA VVREDFERI RQDTRYEKL FEPPKWDVI 
      IRILTPVDK PKSQGRFRK KSDWDTRSR RSSLPTLYE 
      YDSDGGSSV RTITQEPIV HPLRTRRVS RSSYRSEAD 
      LRSMSEVTV DFGRPEDSH SESSSYYPR YYEDSEFEY 
      KSSSSHHPS LARSLSQPS LARSASEFT ERWIAPSRY 
      DTASEFTSP EATPKSQRS SRIQPLHVP RGSGEHSSV 
      WQATASSQV TQRPGMTTQ VVTKEFHSE ASTSFMGAT 
      SKNWFGDND SEASFK
    CDS sequence
    • >TC011068 RA
      atggctcaa tatctagtc tttttcctg gcactgttc 
      ctcttcaaa tcggcgctc tcgcagtcc aactatgcc 
      gaccaggct aacaatatc gagtaccaa ggcaaaggc 
      cttcccgac caagccact ctggatggc aaagtgacg 
      aaactggac gatctcagc cctgttatt ttcctaaac 
      cggaccaaa gccgctctc aactgcgcc gcaggctcg 
      atgcaagtc gagctcaaa ttcaacgat aagttctac 
      ggtattgcc tatgccgat tttgaccgg aattccgcc 
      tgccaggtg tatggcaaa ggtggatac agctacaaa 
      ctcgaattg ccactcaaa ggttgcgga accaaacaa 
      gaccctcaa cgtgttttc acaaataac atcgtcgtg 
      aggttccac ccaggcttg gaaatcgat ggggacgag 
      attattacg attgtgtgc cggtaccca ccgccggtg 
      gcaccactg cccgaccct ttgatcgac ttcagcaaa 
      gaagtacca cctcctgcg cgggtgctt gaaccggcc 
      ttgaaaccc ttccagatt cttctaatc ttctgtggg 
      attctcttc ctgtctttg ctgctgcta ggtttaggt 
      tgttcgtat tattgccta aggaggcgc aatttgaca 
      gtcgtccgg aagcccttc tcgccattg accgacggt 
      tccgaaatc accaaagtc tctggaagc tcacttggc 
      aacttgtcg atgtttgaa ggcttgaag atcccacgg 
      gttagtgcg ccggtggtt gcggcccct ggaagtagt 
      tccgggagt gaaggtcct ctcattagt gatactctc 
      ccgtcggat tacccctcc gagtcccac tcggaggct 
      gaagatgct cgttccctc ccaccttct tcgggtggc 
      agcttcgag aaccaagcc tacgtcgct gatacgttc 
      tacaccgaa ccaccaaga ctccccttg gctaaggaa 
      cctaagttt gatgtccaa gttcgtgtg aaacgagct 
      ccgccctcg cccccaagt ctcagtatc tccgacacg 
      gagagttca gcccggaat ttatccaca atcatggaa 
      cagagggag gaaagtgtt cgctcgatt gattccccc 
      ctccctgtc gaacgcacc cacttcacg tacgtccct 
      gaactgcac cccccaccg aaacacata caaccggca 
      cccacttac aaccgcatt ttgcgaagg caacaagaa 
      ctacaagag gagaggtca attgcatcg ctgaatacc 
      gaaatgact gatacgcac tcaatgacg gagatgatt 
      gatgatagt caccataaa ccagcgccg cctccgcca 
      cccccaatc atcccgcgg caggaatac gtggttgat 
      ttcgccccg cccccgaca ctggaaccc ccaatcgcg 
      gtggtgcac aaaccggaa atcacctca catgttgtg 
      gacgatgtt ttcctgcgg accatcacc gagaagaag 
      actatcgag gatattgaa cgacacaag aggctgatt 
      acggagtac cacacccgg gctaaggcg atccctgag 
      cagaaatgg gacgttacc atcaagaat tatccagtg 
      gagatgcca gaaccccca gaatgggag aacttctct 
      gatatttcc tccgcgtct ggaatgacc ttgacgccg 
      aaaccggaa cgcgccacc atgtcgctc ccaccgcag 
      agtaccatc gacgacaac aaactccca ctgaactct 
      ccagaactc gtcgggagt gtcacacag caaggtacg 
      ttcctcgaa accttcaat atcccactt gagaatccg 
      gaagtgcca aattggagc gtcctgatc cgggtacta 
      caacccgaa caggaggaa ggtccagtg atcgaaagc 
      gccgaaacg tttaattcg caactaacc ctctccgac 
      aaaatgaag tggagacag attataacc accgaatcc 
      actctacgc actctcctg actgaagcc gtggtccgg 
      gaagacttc gaacgcatc cgtcaggac acccgttac 
      gagaaactc ttcgagccg cccaaatgg gacgtcatc 
      atccgaatt ctaaccccg gtggacaaa cccaagagc 
      cagggccgc ttccgcaaa aaatccgac tgggacacg 
      cgcagccgc cgtagcagc ctgccaacg ctctacgag 
      tacgactcc gacggtggt agttccgtg cgtaccatc 
      acccaggaa cccatcgtg cacccactg cgcacccgc 
      cgggtgtcg aggtctagt taccgcagc gaggccgat 
      ttgcgctcc atgtccgag gtgacggtc gactttggc 
      cgtcccgaa gacagccat tccgaaagt tccagttac 
      tatccgcgt tactacgag gattcggag tttgagtat 
      aagagtagc tcctcccac cacccctcc ctcgcgcgg 
      tctctaagc cagccctcg ctggcccgc tcagccagt 
      gaattcacc gagcggtgg attgcgccc tcccgttac 
      gacacggcg agtgagttc actagtcca gaggccacg 
      cccaagtcg cagaggagt agccgcatc caaccactg 
      catgttccc cgaggtagt ggggagcat tcgtccgtg 
      tggcaggcg acagccagc agtcaggtc actcaaagg 
      ccgggaatg accacgcaa gtggtgacg aaagagttc 
      cacagtgaa gcgtcgact agttttatg ggcgcgacc 
      tcgaagaac tggttcggg gataacgac agtgaagct 
      agtttcaaa taa
    mRNA sequence
    • >TC011068 RA
      aaatggttt cgcggcgag atcatgaac ggttgatgg 
      tttccggat ggctcaata tctagtctt tttcctggc 
      actgttcct cttcaaatc ggcgctctc gcagtccaa 
      ctatgccga ccaggctaa caatatcga gtaccaagg 
      caaaggcct tcccgacca agccactct ggatggcaa 
      agtgacgaa actggacga tctcagccc tgttatttt 
      cctaaaccg gaccaaagc cgctctcaa ctgcgccgc 
      aggctcgat gcaagtcga gctcaaatt caacgataa 
      gttctacgg tattgccta tgccgattt tgaccggaa 
      ttccgcctg ccaggtgta tggcaaagg tggatacag 
      ctacaaact cgaattgcc actcaaagg ttgcggaac 
      caaacaaga ccctcaacg tgttttcac aaataacat 
      cgtcgtgag gttccaccc aggcttgga aatcgatgg 
      ggacgagat tattacgat tgtgtgccg gtacccacc 
      gccggtggc accactgcc cgacccttt gatcgactt 
      cagcaaaga agtaccacc tcctgcgcg ggtgcttga 
      accggcctt gaaaccctt ccagattct tctaatctt 
      ctgtgggat tctcttcct gtctttgct gctgctagg 
      tttaggttg ttcgtatta ttgcctaag gaggcgcaa 
      tttgacagt cgtccggaa gcccttctc gccattgac 
      cgacggttc cgaaatcac caaagtctc tggaagctc 
      acttggcaa cttgtcgat gtttgaagg cttgaagat 
      cccacgggt tagtgcgcc ggtggttgc ggcccctgg 
      aagtagttc cgggagtga aggtcctct cattagtga 
      tactctccc gtcggatta cccctccga gtcccactc 
      ggaggctga agatgctcg ttccctccc accttcttc 
      gggtggcag cttcgagaa ccaagccta cgtcgctga 
      tacgttcta caccgaacc accaagact ccccttggc 
      taaggaacc taagtttga tgtccaagt tcgtgtgaa 
      acgagctcc gccctcgcc cccaagtct cagtatctc 
      cgacacgga gagttcagc ccggaattt atccacaat 
      catggaaca gagggagga aagtgttcg ctcgattga 
      ttcccccct ccctgtcga acgcaccca cttcacgta 
      cgtccctga actgcaccc cccaccgaa acacataca 
      accggcacc cacttacaa ccgcatttt gcgaaggca 
      acaagaact acaagagga gaggtcaat tgcatcgct 
      gaataccga aatgactga tacgcactc aatgacgga 
      gatgattga tgatagtca ccataaacc agcgccgcc 
      tccgccacc cccaatcat cccgcggca ggaatacgt 
      ggttgattt cgccccgcc cccgacact ggaaccccc 
      aatcgcggt ggtgcacaa accggaaat cacctcaca 
      tgttgtgga cgatgtttt cctgcggac catcaccga 
      gaagaagac tatcgagga tattgaacg acacaagag 
      gctgattac ggagtacca cacccgggc taaggcgat 
      ccctgagca gaaatggga cgttaccat caagaatta 
      tccagtgga gatgccaga acccccaga atgggagaa 
      cttctctga tatttcctc cgcgtctgg aatgacctt 
      gacgccgaa accggaacg cgccaccat gtcgctccc 
      accgcagag taccatcga cgacaacaa actcccact 
      gaactctcc agaactcgt cgggagtgt cacacagca 
      aggtacgtt cctcgaaac cttcaatat cccacttga 
      gaatccgga agtgccaaa ttggagcgt cctgatccg 
      ggtactaca acccgaaca ggaggaagg tccagtgat 
      cgaaagcgc cgaaacgtt taattcgca actaaccct 
      ctccgacaa aatgaagtg gagacagat tataaccac 
      cgaatccac tctacgcac tctcctgac tgaagccgt 
      ggtccggga agacttcga acgcatccg tcaggacac 
      ccgttacga gaaactctt cgagccgcc caaatggga 
      cgtcatcat ccgaattct aaccccggt ggacaaacc 
      caagagcca gggccgctt ccgcaaaaa atccgactg 
      ggacacgcg cagccgccg tagcagcct gccaacgct 
      ctacgagta cgactccga cggtggtag ttccgtgcg 
      taccatcac ccaggaacc catcgtgca cccactgcg 
      cacccgccg ggtgtcgag gtctagtta ccgcagcga 
      ggccgattt gcgctccat gtccgaggt gacggtcga 
      ctttggccg tcccgaaga cagccattc cgaaagttc 
      cagttacta tccgcgtta ctacgagga ttcggagtt 
      tgagtataa gagtagctc ctcccacca cccctccct 
      cgcgcggtc tctaagcca gccctcgct ggcccgctc 
      agccagtga attcaccga gcggtggat tgcgccctc 
      ccgttacga cacggcgag tgagttcac tagtccaga 
      ggccacgcc caagtcgca gaggagtag ccgcatcca 
      accactgca tgttccccg aggtagtgg ggagcattc 
      gtccgtgtg gcaggcgac agccagcag tcaggtcac 
      tcaaaggcc gggaatgac cacgcaagt ggtgacgaa 
      agagttcca cagtgaagc gtcgactag ttttatggg 
      cgcgacctc gaagaactg gttcgggga taacgacag 
      tgaagctag tttcaaata actgattaa cggaattta 
      ttaacactg cccgaaaaa aattttgtt ttctcgtaa 
      ttttagtaa ctagaatgc tgtgataac gtctgtaag 
      gttacgatt ggccggttt ttggattgt ttgtaaagg 
      gctatcacc cttttgttg acttttcca cgattgttg 
      tggaggact gaacaatcg ggtactact aaatctagt 
      tctaggttc taatctatg ctcaaatct ttggtggtg 
      gtcgatggg cttctttct cctaacctc gctctcgtt 
      ttagttaat tttattttt atacagata tctctaggt 
      gctattaca tttataccg aatgtttgt aacttttta 
      taatgaaat aaattaatt taattaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY