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Gene information
General information
TC011064 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5504533..5517742
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011064
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01795

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head capsule not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    antenna not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011064 RA
      MFKLLGGLK DYLKYQDVI VDSAVFRMH NLFTTALLM 
      ACSLIITAS QYVGNPIQC IVDGLPGHV VNTFCWISS 
      TFTMPDAFR RQVGREVAH PGVANDFGA EDAKKYYTY 
      YQWVCFVLF FQALACYVP KVLWDVFEG GLMKTLSMR 
      LKFGICHED EKNAKKEVI FDYLLTHVR CHNLYALRY 
      FACECLCLI NVIVQLYLM NKFFDGEFL SYGWRVMNF 
      SEQAQEDRM DPMVYVFPR VTKCIFHKY GASGSIQKH 
      DSLCILPLN IVNEKTYIF IWFWFMILA SMLTVLVLY 
      RIAIVASPR LRPRLLNAR HRAIPIEVC RSLCRKIEL 
      GDWWVLMLL GRNMDPMIY REIICELTK RIETRHQN
    CDS sequence
    • >TC011064 RA
      atgttcaag cttttgggc ggtttgaag gactatctc 
      aagtaccag gatgtcatc gtcgacagc gccgtcttc 
      cggatgcac aatctcttc actacggcc ttgctgatg 
      gcctgcagt ttgatcatc acggcctcg caatatgta 
      ggaaatccc atacaatgc atagtggac ggactcccg 
      gggcatgtg gtcaacacc ttctgctgg atctcctcc 
      acgttcacg atgcctgat gccttcagg cggcaggtc 
      ggtcgagaa gttgcccat cctggtgtt gctaacgac 
      ttcggcgcc gaagacgcc aagaaatac tacacctac 
      taccaatgg gtctgcttc gttctgttc ttccaggct 
      ttggcttgt tacgtgccc aaagtgttg tgggacgta 
      tttgaaggg ggccttatg aagacgctg agtatgaga 
      ctgaagttc gggatctgt cacgaggac gagaaaaat 
      gccaagaag gaggtgatt tttgactac ctcctcacc 
      catgttcga tgccacaac ctgtacgcc ctgcggtac 
      ttcgcctgc gagtgcctc tgcctcatc aacgtgatc 
      gtccagctg tacctcatg aacaagttc ttcgacggc 
      gaattcctc tcgtacggc tggcgcgtg atgaacttc 
      tccgagcag gcgcaggag gaccgcatg gaccccatg 
      gtgtacgtc ttcccgcgc gtcaccaag tgcatcttc 
      cacaagtac ggggcctcc ggctcgatc cagaagcac 
      gacagcttg tgcatcctc ccgctgaac atcgtcaac 
      gagaagacc tacatcttc atctggttc tggttcatg 
      atcctggcc agcatgctc acagtgctg gtcttgtac 
      cggatcgcg atcgtggcc tcgcccagg ctgcggcct 
      aggctgctc aacgcccga cacagggcg attcctatc 
      gaggtttgc cgatcgctg tgtcggaaa atcgagctc 
      ggggattgg tgggttctg atgctcctg ggccgaaat 
      atggacccg atgatctac agggagatt atctgcgag 
      ctgaccaag aggattgag accaggcac cagaattga
    mRNA sequence
    • >TC011064 RA
      cccatccaa tccttgtgt cagccccct cgcttgtct 
      tggtcgact gcacctctg gtgggggaa gcagcaaca 
      aatcgaggg gggcagcac tcagtagca agttacgcc 
      acggagtgt tccgtcgct tccatcgta aacaattcc 
      atgtgactg aagatgaca agtgatagg tgaagtgaa 
      agtggcaaa atgttcaag cttttgggc ggtttgaag 
      gactatctc aagtaccag gatgtcatc gtcgacagc 
      gccgtcttc cggatgcac aatctcttc actacggcc 
      ttgctgatg gcctgcagt ttgatcatc acggcctcg 
      caatatgta ggaaatccc atacaatgc atagtggac 
      ggactcccg gggcatgtg gtcaacacc ttctgctgg 
      atctcctcc acgttcacg atgcctgat gccttcagg 
      cggcaggtc ggtcgagaa gttgcccat cctggtgtt 
      gctaacgac ttcggcgcc gaagacgcc aagaaatac 
      tacacctac taccaatgg gtctgcttc gttctgttc 
      ttccaggct ttggcttgt tacgtgccc aaagtgttg 
      tgggacgta tttgaaggg ggccttatg aagacgctg 
      agtatgaga ctgaagttc gggatctgt cacgaggac 
      gagaaaaat gccaagaag gaggtgatt tttgactac 
      ctcctcacc catgttcga tgccacaac ctgtacgcc 
      ctgcggtac ttcgcctgc gagtgcctc tgcctcatc 
      aacgtgatc gtccagctg tacctcatg aacaagttc 
      ttcgacggc gaattcctc tcgtacggc tggcgcgtg 
      atgaacttc tccgagcag gcgcaggag gaccgcatg 
      gaccccatg gtgtacgtc ttcccgcgc gtcaccaag 
      tgcatcttc cacaagtac ggggcctcc ggctcgatc 
      cagaagcac gacagcttg tgcatcctc ccgctgaac 
      atcgtcaac gagaagacc tacatcttc atctggttc 
      tggttcatg atcctggcc agcatgctc acagtgctg 
      gtcttgtac cggatcgcg atcgtggcc tcgcccagg 
      ctgcggcct aggctgctc aacgcccga cacagggcg 
      attcctatc gaggtttgc cgatcgctg tgtcggaaa 
      atcgagctc ggggattgg tgggttctg atgctcctg 
      ggccgaaat atggacccg atgatctac agggagatt 
      atctgcgag ctgaccaag aggattgag accaggcac 
      cagaattga gggacttgg cggtttaga ataatactc 
      agagacttt tatttattt ttgtcaggt ttggaccgg 
      tcgaaacaa aattctgtg attacgccg actggtagt 
      ttcaatcaa tgacatatc acttttgta aatttatac 
      aatttttat taatttgta agagcacag cttaggtta 
      aggtaattt tagacgatt ttacttctg tctttatta 
      ttgtataaa taaacgaaa gaaagacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY