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Gene information
General information
TC011059 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5431147..5436529
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011059
    iBeetle screen iB_01794

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 leg musculature femural musculature randomnot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: 50-80 % of the larvae seemed to have no muscles in the femur & tibiotarsus, randomly in 1 up to all 6 legs.
    leg musculature tb1-ptar1 randomnot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages random shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs tibiotarsus shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs pretarsus shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen centraldorsoventral-axis increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011059 RA
      MIHSLFIIN SSGDVFLEK HWRSVISRS VCDYYLEAQ 
      RANPNDIAP VIATPHHYL ISIQRSGVS FVAVCMEEI 
      PPLFVIEFL HRVVDTFQD YFSDCTESI IKENYVVIY 
      ELLDEMLDN GFPLATESN ILKELIKPP NILRTIANT 
      VTGKTNVSE VLPTGQLSN IPWRRTGVK YTNNEAYFD 
      VIEEVDAII DKSGSTVFA EIQGYIDCC IKLSGMPDL 
      TLSFMNPRL FDDVSFHPC VRFKRWEAE RVLSFIPPD 
      GNFRLISYH ISSQSVVAI PIYVRHNLS IKSGEQGRL 
      DLTVGPKQT LGRTVEAVK IEVLMPKCI LNCVLTANQ 
      GKYNFDPVS KILHWDIGR IDVTKLPNI RGSVSIASG 
      ANTAEINPS INVHFTINQ LAVSGLKVN RLDMYGEKY 
      KPFKGVKYI TKAGRFQIR M
    CDS sequence
    • >TC011059 RA
      atgattcac agtttgttt attattaat tcgtcaggt 
      gacgttttc ctggaaaaa cattggcgc agtgtgatt 
      tcacgatcg gtttgtgac tattattta gaggcccaa 
      agggcgaac ccgaatgac atagccccc gtgatagcc 
      accccccat cactacttg atctcaatc cagcgaagt 
      ggtgttagt tttgtcgcc gtgtgtatg gaggagatc 
      cccccatta ttcgtgatt gaatttttg cacagagtt 
      gttgacacg ttccaagat tatttctcc gactgcacc 
      gaatcgata attaaagag aattatgtc gtaatttac 
      gaattgcta gacgaaatg ttggataat gggttccct 
      ctcgcaact gagagtaat attttgaaa gaattaatc 
      aagccgcct aatattcta cgaacaatt gccaacact 
      gtcacaggg aaaaccaat gtgagcgaa gtattgcca 
      actggacaa ttgtcaaat attccatgg cgacgtact 
      ggagtcaaa tacacaaat aacgaggcc tactttgat 
      gtgatcgaa gaagtcgac gcgattata gataagtca 
      ggctccacg gtttttgcc gaaatccag ggttatatc 
      gactgttgc atcaaactg agcggcatg cccgacttg 
      actctttcg ttcatgaac ccgcgcctt ttcgacgac 
      gtcagtttc cacccttgt gtgcgtttt aagcggtgg 
      gaggccgaa cgagtgttg tcatttatt ccaccagat 
      ggcaatttc cgcctcatt tcgtaccac ataagcagt 
      caaagcgtc gtcgcgatc ccgatttac gtccgacac 
      aacctgtcg atcaaatcg ggagagcaa ggacgtctg 
      gacttgaca gtgggcccc aaacagacc ctggggcga 
      acggtcgag gccgttaaa atcgaggtg ctgatgccg 
      aaatgcatc ctcaattgt gtgctcact gcgaatcag 
      gggaagtac aactttgac ccggtttcg aaaattttg 
      cactgggat attggcagg attgacgtc accaaactg 
      ccgaatatt aggggaagt gtgtcaatc gccagtggg 
      gccaacact gcggagatt aatccgtcc ataaatgtg 
      cattttacg ataaatcag ctcgcagtg agcggattg 
      aaagttaat agacttgac atgtatgga gagaagtat 
      aagcctttt aagggggtg aaatacata acgaaagcg 
      ggacgattc caaatcagg atgtga
    mRNA sequence
    • >TC011059 RA
      caaatgagc gacttttat tagctgacg tgtttttat 
      ttacttagg aaaatgcca atggtgata ttccatcac 
      agttaatac aattattta cataaaagg gcttaaatt 
      aagtttgga gattaataa gtaattttt tgcctttgt 
      gcacctcaa gtgtattgt cacttgtca aaagtttgt 
      cgcccctaa cctcaaaac ccaaaatga ttcacagtt 
      tgtttatta ttaattcgt caggtgacg ttttcctgg 
      aaaaacatt ggcgcagtg tgatttcac gatcggttt 
      gtgactatt atttagagg cccaaaggg cgaacccga 
      atgacatag cccccgtga tagccaccc cccatcact 
      acttgatct caatccagc gaagtggtg ttagttttg 
      tcgccgtgt gtatggagg agatccccc cattattcg 
      tgattgaat ttttgcaca gagttgttg acacgttcc 
      aagattatt tctccgact gcaccgaat cgataatta 
      aagagaatt atgtcgtaa tttacgaat tgctagacg 
      aaatgttgg ataatgggt tccctctcg caactgaga 
      gtaatattt tgaaagaat taatcaagc cgcctaata 
      ttctacgaa caattgcca acactgtca cagggaaaa 
      ccaatgtga gcgaagtat tgccaactg gacaattgt 
      caaatattc catggcgac gtactggag tcaaataca 
      caaataacg aggcctact ttgatgtga tcgaagaag 
      tcgacgcga ttatagata agtcaggct ccacggttt 
      ttgccgaaa tccagggtt atatcgact gttgcatca 
      aactgagcg gcatgcccg acttgactc tttcgttca 
      tgaacccgc gccttttcg acgacgtca gtttccacc 
      cttgtgtgc gttttaagc ggtgggagg ccgaacgag 
      tgttgtcat ttattccac cagatggca atttccgcc 
      tcatttcgt accacataa gcagtcaaa gcgtcgtcg 
      cgatcccga tttacgtcc gacacaacc tgtcgatca 
      aatcgggag agcaaggac gtctggact tgacagtgg 
      gccccaaac agaccctgg ggcgaacgg tcgaggccg 
      ttaaaatcg aggtgctga tgccgaaat gcatcctca 
      attgtgtgc tcactgcga atcagggga agtacaact 
      ttgacccgg tttcgaaaa ttttgcact gggatattg 
      gcaggattg acgtcacca aactgccga atattaggg 
      gaagtgtgt caatcgcca gtggggcca acactgcgg 
      agattaatc cgtccataa atgtgcatt ttacgataa 
      atcagctcg cagtgagcg gattgaaag ttaatagac 
      ttgacatgt atggagaga agtataagc cttttaagg 
      gggtgaaat acataacga aagcgggac gattccaaa 
      tcaggatgt gagaaaaat gtgcaagaa ccgattttt 
      atttttata taaataagt tgcaaatgt attatctca 
      ttgtcaata aatgttgat tatttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY