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Gene information
General information
TC011058 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5414827..5430141
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011058
    iBeetle screen iB_01793

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection

    Pupal morphology
    remarks: later than schedule,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011058 RA
      MAGNSGMEQ LIPIVNKLQ DAFTQLGVH MSLDLPQIA 
VVGGQSAGK SSVLENFVG KDFLPRGSG IVTRRPLIL 
QLINSNSEY AEFLHCKGK KFVDFDEVR REIEAETDR 
VTGSNKGIS NIPINLRVY SPHVLNLTL IDLPGLTKV 
PIGDQPLDI EQQIKGMIM QFIKRESCL ILAVTPANT 
DLANSDALK LAKEVDPQG LRTIGVITK LDLMDEGTD 
ARDVLENKL LPLRRGYIG VVNRSQKDI DGRKDIKAA 
MAAERQFFL SHPSYRHLA DRLGTPYLQ RVLNQQLTN 
HIRDTLPGL RDKLQKQLL TLEKDVEQF KHFRPDDPA 
IKTKAMLQM IQQLQTDFE RTIEGSGSA QINTNELSG 
GAKINRLFH ERFPFEIVK MEFDEKELR REIAFAIRN 
IHGIRVGLF TPDMAFEAI VKKQIARLK EPSLKCTDL 
VVTELSNVV RMCTDKMSR YPRLREEAE RIITTHIRQ 
REQYCKEQL CLLIDCELA YMNTNHEDF IGFANAQNQ 
SENASKTGS RGTLGNQVI RKGYMCIHN LGIMKGGSR 
DYWFVLTSE NISWYKDEE EREKKYMLP LDGLKLRDI 
EQGFMSRRH MFALFNPDG RNVYKDYKQ LELSCETLD 
EVDSWKASF LRAGVYPEK QTEQVNGEE AGGENVSSS 
MDPQLERQV ETIRNLVDS YMRIVTKTT RDLVPKTIM 
YMIINHTKD FINGELLAH IYASGDQSQ MMEEAPEEA 
QKREEMLRM YHACKEALH IIGDVSMAT VSTPVPPPV 
KNDWLASGL ENPRLSPPS PGGPRKTAP NMGQVGSTG 
SLGSRAPPP PPSSGRPAP AIPNRPGGG APPMPPGRP 
GGQALPAPL VPTRIGGQG PQGGVQVPP QVQMAVGRA 
VTNAAINEL SNAFKFHNR PVPNIPPRI PDRPNPGRP 
N
    CDS sequence
    • >TC011058 RA
      atggcagga aactccgga atggagcag cttattccc 
atcgtgaat aagctgcaa gacgctttc acccagttg 
ggggtgcac atgagcctg gatctgccc caaatcgct 
gttgtgggc ggacagtcg gctggaaaa agctccgtt 
ttggagaat ttcgtcgga aaagacttc cttccgaga 
ggatccggg attgtcacc agaaggccc cttatccta 
caattgatt aactccaat tcggaatat gctgaattt 
ctccactgt aaaggcaaa aaattcgtc gatttcgac 
gaagtgcgt cgcgagatt gaagcggaa acggaccga 
gtcaccgga agtaacaaa ggaatatcg aatattccc 
atcaacttg agggtgtac tcaccccat gtgttaaat 
ttaaccctg atcgatcta ccgggcttg acaaaagtg 
cccattggc gatcaaccc ctcgacatc gaacaacaa 
atcaaaggg atgataatg cagttcatc aaacgtgaa 
tcgtgtctc atcttggcc gtaactccc gccaacacc 
gatttggcc aactcggat gccctcaaa ctggccaaa 
gaggtcgat cctcaaggt cttcggacg attggtgtt 
atcacgaag cttgatttg atggatgaa gggaccgac 
gctcgggac gtgctagaa aataagctt ttgcccttg 
agaaggggt tatataggt gtggtgaat cgctcacag 
aaggatatc gacgggcgg aaggatatc aaggcggcg 
atggcggcc gaaaggcaa ttctttttg tcgcatccc 
tcgtataga catcttgcc gataggttg ggaacaccc 
tatttgcaa agggtgttg aatcaacaa ttgaccaac 
cacataagg gatacgtta ccaggattg agggataaa 
ttgcagaag cagttgttg acgcttgag aaggatgtc 
gagcagttt aagcacttc aggcccgat gatcctgcg 
attaaaacc aaggcgatg ttacagatg atccaacaa 
ctccaaacc gacttcgaa cgcacgatc gaaggttcc 
ggttcggct caaatcaac acaaatgaa ttatcaggt 
ggtgcaaaa atcaatcgt ttattccac gagcgtttt 
cccttcgaa atcgtcaaa atggaattt gatgaaaag 
gaactgcga cgagaaatc gctttcgca attcgcaat 
attcacggt atcagagtg gggcttttc acccccgat 
atggcattc gaagcaatc gttaaaaaa caaattgct 
cgattgaaa gaaccctca ctaaaatgc accgatcta 
gtggtcact gaattgtca aatgttgtg cgcatgtgc 
actgataag atgtcgcgc tatccgcga ctaagagag 
gaggcagaa cgaatcatc accacacac atcagacag 
agagagcaa tattgcaag gaacagttg tgcttactt 
atcgactgc gaactggct tatatgaac acaaaccat 
gaggatttt atcggtttt gccaatgcc caaaatcag 
tccgagaat gcttctaag actgggtca cgtggcact 
ttgggcaat caagtgatc cggaaaggc tacatgtgt 
atccataat ttgggtata atgaaagga gggtcacgt 
gattattgg ttcgttctc acgtctgaa aacatctca 
tggtataag gatgaggaa gagagggag aaaaaatac 
atgttgcca ttggatggc ttaaaactg agagacata 
gaacagggt tttatgtca cgacgacac atgtttgcg 
ttgtttaat cctgacggt cgtaacgtc tacaaggat 
tacaaacaa ctcgaattg agttgtgag actttggat 
gaagtcgat tcgtggaag gcgtcgttc ttgagggcg 
ggggtttac ccggaaaag cagacggaa caagtgaac 
ggcgaagag gccggtggt gagaacgtg agcagttca 
atggacccc caactcgaa cgtcaagtg gaaacaatt 
cgaaatttg gtcgacagt tacatgcga atcgtgaca 
aaaacaacc cgagatcta gtaccaaaa acaataatg 
tacatgatc ataaaccac accaaagac ttcataaac 
ggcgaactt ttagcccac atttacgcc agtggtgat 
cagtcccaa atgatggaa gaagctccc gaagaggcc 
caaaaacgc gaagaaatg ctccgtatg taccacgcg 
tgcaaggag gcgttgcac atcatcggt gacgtttcc 
atggcgacg gtttccacg cccgtgccg cctccggtg 
aaaaatgat tggttggcg agcggcctt gagaatcca 
cgtttgtcg ccaccgagt ccaggcgga cccagaaaa 
accgccccc aatatggga caagtcggc agcactggt 
tcgttaggt tcgagggct ccgccgccg cccccaagc 
tccgggaga ccggccccc gccattccg aaccgcccc 
gggggcgga gcaccccca atgccccca ggcaggccg 
ggaggacaa gccttgccg gcgcccctc gtccccact 
cgaattggg ggccaagga ccgcaaggg ggcgtccaa 
gtgcctcct caggtgcag atggcagtc ggacgcgct 
gttaccaat gctgctatc aacgaactc tccaatgcc 
ttcaagttc cataatcgg ccagttcca aacatacct 
ccaagaata ccagacagg ccaaacccg ggacgacca 
aattaa
    mRNA sequence
    • >TC011058 RA
      tccgaattc aaaaatccc attttatca gcttgagtt 
tgacattta ttcttgaaa aaatcgttc ccttatcac 
tgtaatcac tgttctatc gcccaattc tccccgaaa 
cgtgtaatt ttgtgatat atctcgtcg tgaagtgta 
aatggatga gtcatgcgc gttcagtta tgattcggc 
catgttccc aagtgtata ttgttaaag tgtcgaagt 
gaatttagt tgttttcga gggaaatta gcacagttc 
cggcctaaa aatggcagg aaactccgg aatggagca 
gcttattcc catcgtgaa taagctgca agacgcttt 
cacccagtt gggggtgca catgagcct ggatctgcc 
ccaaatcgc tgttgtggg cggacagtc ggctggaaa 
aagctccgt tttggagaa tttcgtcgg aaaagactt 
ccttccgag aggatccgg gattgtcac cagaaggcc 
ccttatcct acaattgat taactccaa ttcggaata 
tgctgaatt tctccactg taaaggcaa aaaattcgt 
cgatttcga cgaagtgcg tcgcgagat tgaagcgga 
aacggaccg agtcaccgg aagtaacaa aggaatatc 
gaatattcc catcaactt gagggtgta ctcacccca 
tgtgttaaa tttaaccct gatcgatct accgggctt 
gacaaaagt gcccattgg cgatcaacc cctcgacat 
cgaacaaca aatcaaagg gatgataat gcagttcat 
caaacgtga atcgtgtct catcttggc cgtaactcc 
cgccaacac cgatttggc caactcgga tgccctcaa 
actggccaa agaggtcga tcctcaagg tcttcggac 
gattggtgt tatcacgaa gcttgattt gatggatga 
agggaccga cgctcggga cgtgctaga aaataagct 
tttgccctt gagaagggg ttatatagg tgtggtgaa 
tcgctcaca gaaggatat cgacgggcg gaaggatat 
caaggcggc gatggcggc cgaaaggca attcttttt 
gtcgcatcc ctcgtatag acatcttgc cgataggtt 
gggaacacc ctatttgca aagggtgtt gaatcaaca 
attgaccaa ccacataag ggatacgtt accaggatt 
gagggataa attgcagaa gcagttgtt gacgcttga 
gaaggatgt cgagcagtt taagcactt caggcccga 
tgatcctgc gattaaaac caaggcgat gttacagat 
gatccaaca actccaaac cgacttcga acgcacgat 
cgaaggttc cggttcggc tcaaatcaa cacaaatga 
attatcagg tggtgcaaa aatcaatcg tttattcca 
cgagcgttt tcccttcga aatcgtcaa aatggaatt 
tgatgaaaa ggaactgcg acgagaaat cgctttcgc 
aattcgcaa tattcacgg tatcagagt ggggctttt 
cacccccga tatggcatt cgaagcaat cgttaaaaa 
acaaattgc tcgattgaa agaaccctc actaaaatg 
caccgatct agtggtcac tgaattgtc aaatgttgt 
gcgcatgtg cactgataa gatgtcgcg ctatccgcg 
actaagaga ggaggcaga acgaatcat caccacaca 
catcagaca gagagagca atattgcaa ggaacagtt 
gtgcttact tatcgactg cgaactggc ttatatgaa 
cacaaacca tgaggattt tatcggttt tgccaatgc 
ccaaaatca gtccgagaa tgcttctaa gactgggtc 
acgtggcac tttgggcaa tcaagtgat ccggaaagg 
ctacatgtg tatccataa tttgggtat aatgaaagg 
agggtcacg tgattattg gttcgttct cacgtctga 
aaacatctc atggtataa ggatgagga agagaggga 
gaaaaaata catgttgcc attggatgg cttaaaact 
gagagacat agaacaggg ttttatgtc acgacgaca 
catgtttgc gttgtttaa tcctgacgg tcgtaacgt 
ctacaagga ttacaaaca actcgaatt gagttgtga 
gactttgga tgaagtcga ttcgtggaa ggcgtcgtt 
cttgagggc gggggttta cccggaaaa gcagacgga 
acaagtgaa cggcgaaga ggccggtgg tgagaacgt 
gagcagttc aatggaccc ccaactcga acgtcaagt 
ggaaacaat tcgaaattt ggtcgacag ttacatgcg 
aatcgtgac aaaaacaac ccgagatct agtaccaaa 
aacaataat gtacatgat cataaacca caccaaaga 
cttcataaa cggcgaact tttagccca catttacgc 
cagtggtga tcagtccca aatgatgga agaagctcc 
cgaagaggc ccaaaaacg cgaagaaat gctccgtat 
gtaccacgc gtgcaagga ggcgttgca catcatcgg 
tgacgtttc catggcgac ggtttccac gcccgtgcc 
gcctccggt gaaaaatga ttggttggc gagcggcct 
tgagaatcc acgtttgtc gccaccgag tccaggcgg 
acccagaaa aaccgcccc caatatggg acaagtcgg 
cagcactgg ttcgttagg ttcgagggc tccgccgcc 
gcccccaag ctccgggag accggcccc cgccattcc 
gaaccgccc cgggggcgg agcaccccc aatgccccc 
aggcaggcc gggaggaca agccttgcc ggcgcccct 
cgtccccac tcgaattgg gggccaagg accgcaagg 
gggcgtcca agtgcctcc tcaggtgca gatggcagt 
cggacgcgc tgttaccaa tgctgctat caacgaact 
ctccaatgc cttcaagtt ccataatcg gccagttcc 
aaacatacc tccaagaat accagacag gccaaaccc 
gggacgacc aaattaagt tctagtcaa aattgaaac 
gatgggacc aacaaggtt aatttagtg attgttaag 
acttggcgc tttttcagt acaatgaca gtaatttgt 
acctagtct aatgtatag tattacccc cagtcccgc 
ttttgtaca tatctgtat tcaccattt gtaatatat 
tgtttattt ttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY