| General information | |||
| TC011058 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 5414827..5430141 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011058 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01793 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 10 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC011058 RA
MAGNSGMEQ LIPIVNKLQ DAFTQLGVH MSLDLPQIA VVGGQSAGK SSVLENFVG KDFLPRGSG IVTRRPLIL QLINSNSEY AEFLHCKGK KFVDFDEVR REIEAETDR VTGSNKGIS NIPINLRVY SPHVLNLTL IDLPGLTKV PIGDQPLDI EQQIKGMIM QFIKRESCL ILAVTPANT DLANSDALK LAKEVDPQG LRTIGVITK LDLMDEGTD ARDVLENKL LPLRRGYIG VVNRSQKDI DGRKDIKAA MAAERQFFL SHPSYRHLA DRLGTPYLQ RVLNQQLTN HIRDTLPGL RDKLQKQLL TLEKDVEQF KHFRPDDPA IKTKAMLQM IQQLQTDFE RTIEGSGSA QINTNELSG GAKINRLFH ERFPFEIVK MEFDEKELR REIAFAIRN IHGIRVGLF TPDMAFEAI VKKQIARLK EPSLKCTDL VVTELSNVV RMCTDKMSR YPRLREEAE RIITTHIRQ REQYCKEQL CLLIDCELA YMNTNHEDF IGFANAQNQ SENASKTGS RGTLGNQVI RKGYMCIHN LGIMKGGSR DYWFVLTSE NISWYKDEE EREKKYMLP LDGLKLRDI EQGFMSRRH MFALFNPDG RNVYKDYKQ LELSCETLD EVDSWKASF LRAGVYPEK QTEQVNGEE AGGENVSSS MDPQLERQV ETIRNLVDS YMRIVTKTT RDLVPKTIM YMIINHTKD FINGELLAH IYASGDQSQ MMEEAPEEA QKREEMLRM YHACKEALH IIGDVSMAT VSTPVPPPV KNDWLASGL ENPRLSPPS PGGPRKTAP NMGQVGSTG SLGSRAPPP PPSSGRPAP AIPNRPGGG APPMPPGRP GGQALPAPL VPTRIGGQG PQGGVQVPP QVQMAVGRA VTNAAINEL SNAFKFHNR PVPNIPPRI PDRPNPGRP N
- >TC011058 RA
atggcagga aactccgga atggagcag cttattccc atcgtgaat aagctgcaa gacgctttc acccagttg ggggtgcac atgagcctg gatctgccc caaatcgct gttgtgggc ggacagtcg gctggaaaa agctccgtt ttggagaat ttcgtcgga aaagacttc cttccgaga ggatccggg attgtcacc agaaggccc cttatccta caattgatt aactccaat tcggaatat gctgaattt ctccactgt aaaggcaaa aaattcgtc gatttcgac gaagtgcgt cgcgagatt gaagcggaa acggaccga gtcaccgga agtaacaaa ggaatatcg aatattccc atcaacttg agggtgtac tcaccccat gtgttaaat ttaaccctg atcgatcta ccgggcttg acaaaagtg cccattggc gatcaaccc ctcgacatc gaacaacaa atcaaaggg atgataatg cagttcatc aaacgtgaa tcgtgtctc atcttggcc gtaactccc gccaacacc gatttggcc aactcggat gccctcaaa ctggccaaa gaggtcgat cctcaaggt cttcggacg attggtgtt atcacgaag cttgatttg atggatgaa gggaccgac gctcgggac gtgctagaa aataagctt ttgcccttg agaaggggt tatataggt gtggtgaat cgctcacag aaggatatc gacgggcgg aaggatatc aaggcggcg atggcggcc gaaaggcaa ttctttttg tcgcatccc tcgtataga catcttgcc gataggttg ggaacaccc tatttgcaa agggtgttg aatcaacaa ttgaccaac cacataagg gatacgtta ccaggattg agggataaa ttgcagaag cagttgttg acgcttgag aaggatgtc gagcagttt aagcacttc aggcccgat gatcctgcg attaaaacc aaggcgatg ttacagatg atccaacaa ctccaaacc gacttcgaa cgcacgatc gaaggttcc ggttcggct caaatcaac acaaatgaa ttatcaggt ggtgcaaaa atcaatcgt ttattccac gagcgtttt cccttcgaa atcgtcaaa atggaattt gatgaaaag gaactgcga cgagaaatc gctttcgca attcgcaat attcacggt atcagagtg gggcttttc acccccgat atggcattc gaagcaatc gttaaaaaa caaattgct cgattgaaa gaaccctca ctaaaatgc accgatcta gtggtcact gaattgtca aatgttgtg cgcatgtgc actgataag atgtcgcgc tatccgcga ctaagagag gaggcagaa cgaatcatc accacacac atcagacag agagagcaa tattgcaag gaacagttg tgcttactt atcgactgc gaactggct tatatgaac acaaaccat gaggatttt atcggtttt gccaatgcc caaaatcag tccgagaat gcttctaag actgggtca cgtggcact ttgggcaat caagtgatc cggaaaggc tacatgtgt atccataat ttgggtata atgaaagga gggtcacgt gattattgg ttcgttctc acgtctgaa aacatctca tggtataag gatgaggaa gagagggag aaaaaatac atgttgcca ttggatggc ttaaaactg agagacata gaacagggt tttatgtca cgacgacac atgtttgcg ttgtttaat cctgacggt cgtaacgtc tacaaggat tacaaacaa ctcgaattg agttgtgag actttggat gaagtcgat tcgtggaag gcgtcgttc ttgagggcg ggggtttac ccggaaaag cagacggaa caagtgaac ggcgaagag gccggtggt gagaacgtg agcagttca atggacccc caactcgaa cgtcaagtg gaaacaatt cgaaatttg gtcgacagt tacatgcga atcgtgaca aaaacaacc cgagatcta gtaccaaaa acaataatg tacatgatc ataaaccac accaaagac ttcataaac ggcgaactt ttagcccac atttacgcc agtggtgat cagtcccaa atgatggaa gaagctccc gaagaggcc caaaaacgc gaagaaatg ctccgtatg taccacgcg tgcaaggag gcgttgcac atcatcggt gacgtttcc atggcgacg gtttccacg cccgtgccg cctccggtg aaaaatgat tggttggcg agcggcctt gagaatcca cgtttgtcg ccaccgagt ccaggcgga cccagaaaa accgccccc aatatggga caagtcggc agcactggt tcgttaggt tcgagggct ccgccgccg cccccaagc tccgggaga ccggccccc gccattccg aaccgcccc gggggcgga gcaccccca atgccccca ggcaggccg ggaggacaa gccttgccg gcgcccctc gtccccact cgaattggg ggccaagga ccgcaaggg ggcgtccaa gtgcctcct caggtgcag atggcagtc ggacgcgct gttaccaat gctgctatc aacgaactc tccaatgcc ttcaagttc cataatcgg ccagttcca aacatacct ccaagaata ccagacagg ccaaacccg ggacgacca aattaa
- >TC011058 RA
tccgaattc aaaaatccc attttatca gcttgagtt tgacattta ttcttgaaa aaatcgttc ccttatcac tgtaatcac tgttctatc gcccaattc tccccgaaa cgtgtaatt ttgtgatat atctcgtcg tgaagtgta aatggatga gtcatgcgc gttcagtta tgattcggc catgttccc aagtgtata ttgttaaag tgtcgaagt gaatttagt tgttttcga gggaaatta gcacagttc cggcctaaa aatggcagg aaactccgg aatggagca gcttattcc catcgtgaa taagctgca agacgcttt cacccagtt gggggtgca catgagcct ggatctgcc ccaaatcgc tgttgtggg cggacagtc ggctggaaa aagctccgt tttggagaa tttcgtcgg aaaagactt ccttccgag aggatccgg gattgtcac cagaaggcc ccttatcct acaattgat taactccaa ttcggaata tgctgaatt tctccactg taaaggcaa aaaattcgt cgatttcga cgaagtgcg tcgcgagat tgaagcgga aacggaccg agtcaccgg aagtaacaa aggaatatc gaatattcc catcaactt gagggtgta ctcacccca tgtgttaaa tttaaccct gatcgatct accgggctt gacaaaagt gcccattgg cgatcaacc cctcgacat cgaacaaca aatcaaagg gatgataat gcagttcat caaacgtga atcgtgtct catcttggc cgtaactcc cgccaacac cgatttggc caactcgga tgccctcaa actggccaa agaggtcga tcctcaagg tcttcggac gattggtgt tatcacgaa gcttgattt gatggatga agggaccga cgctcggga cgtgctaga aaataagct tttgccctt gagaagggg ttatatagg tgtggtgaa tcgctcaca gaaggatat cgacgggcg gaaggatat caaggcggc gatggcggc cgaaaggca attcttttt gtcgcatcc ctcgtatag acatcttgc cgataggtt gggaacacc ctatttgca aagggtgtt gaatcaaca attgaccaa ccacataag ggatacgtt accaggatt gagggataa attgcagaa gcagttgtt gacgcttga gaaggatgt cgagcagtt taagcactt caggcccga tgatcctgc gattaaaac caaggcgat gttacagat gatccaaca actccaaac cgacttcga acgcacgat cgaaggttc cggttcggc tcaaatcaa cacaaatga attatcagg tggtgcaaa aatcaatcg tttattcca cgagcgttt tcccttcga aatcgtcaa aatggaatt tgatgaaaa ggaactgcg acgagaaat cgctttcgc aattcgcaa tattcacgg tatcagagt ggggctttt cacccccga tatggcatt cgaagcaat cgttaaaaa acaaattgc tcgattgaa agaaccctc actaaaatg caccgatct agtggtcac tgaattgtc aaatgttgt gcgcatgtg cactgataa gatgtcgcg ctatccgcg actaagaga ggaggcaga acgaatcat caccacaca catcagaca gagagagca atattgcaa ggaacagtt gtgcttact tatcgactg cgaactggc ttatatgaa cacaaacca tgaggattt tatcggttt tgccaatgc ccaaaatca gtccgagaa tgcttctaa gactgggtc acgtggcac tttgggcaa tcaagtgat ccggaaagg ctacatgtg tatccataa tttgggtat aatgaaagg agggtcacg tgattattg gttcgttct cacgtctga aaacatctc atggtataa ggatgagga agagaggga gaaaaaata catgttgcc attggatgg cttaaaact gagagacat agaacaggg ttttatgtc acgacgaca catgtttgc gttgtttaa tcctgacgg tcgtaacgt ctacaagga ttacaaaca actcgaatt gagttgtga gactttgga tgaagtcga ttcgtggaa ggcgtcgtt cttgagggc gggggttta cccggaaaa gcagacgga acaagtgaa cggcgaaga ggccggtgg tgagaacgt gagcagttc aatggaccc ccaactcga acgtcaagt ggaaacaat tcgaaattt ggtcgacag ttacatgcg aatcgtgac aaaaacaac ccgagatct agtaccaaa aacaataat gtacatgat cataaacca caccaaaga cttcataaa cggcgaact tttagccca catttacgc cagtggtga tcagtccca aatgatgga agaagctcc cgaagaggc ccaaaaacg cgaagaaat gctccgtat gtaccacgc gtgcaagga ggcgttgca catcatcgg tgacgtttc catggcgac ggtttccac gcccgtgcc gcctccggt gaaaaatga ttggttggc gagcggcct tgagaatcc acgtttgtc gccaccgag tccaggcgg acccagaaa aaccgcccc caatatggg acaagtcgg cagcactgg ttcgttagg ttcgagggc tccgccgcc gcccccaag ctccgggag accggcccc cgccattcc gaaccgccc cgggggcgg agcaccccc aatgccccc aggcaggcc gggaggaca agccttgcc ggcgcccct cgtccccac tcgaattgg gggccaagg accgcaagg gggcgtcca agtgcctcc tcaggtgca gatggcagt cggacgcgc tgttaccaa tgctgctat caacgaact ctccaatgc cttcaagtt ccataatcg gccagttcc aaacatacc tccaagaat accagacag gccaaaccc gggacgacc aaattaagt tctagtcaa aattgaaac gatgggacc aacaaggtt aatttagtg attgttaag acttggcgc tttttcagt acaatgaca gtaatttgt acctagtct aatgtatag tattacccc cagtcccgc ttttgtaca tatctgtat tcaccattt gtaatatat tgtttattt ttat
