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Gene information
General information
TC011058 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid75: 1112146..1127460
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011058
    iBeetle screen iB_01793
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011058 RA
      MAGNSGMEQ LIPIVNKLQ DAFTQLGVH MSLDLPQIA 
      VVGGQSAGK SSVLENFVG KDFLPRGSG IVTRRPLIL 
      QLINSNSEY AEFLHCKGK KFVDFDEVR REIEAETDR 
      VTGSNKGIS NIPINLRVY SPHVLNLTL IDLPGLTKV 
      PIGDQPLDI EQQIKGMIM QFIKRESCL ILAVTPANT 
      DLANSDALK LAKEVDPQG LRTIGVITK LDLMDEGTD 
      ARDVLENKL LPLRRGYIG VVNRSQKDI DGRKDIKAA 
      MAAERQFFL SHPSYRHLA DRLGTPYLQ RVLNQQLTN 
      HIRDTLPGL RDKLQKQLL TLEKDVEQF KHFRPDDPA 
      IKTKAMLQM IQQLQTDFE RTIEGSGSA QINTNELSG 
      GAKINRLFH ERFPFEIVK MEFDEKELR REIAFAIRN 
      IHGIRVGLF TPDMAFEAI VKKQIARLK EPSLKCTDL 
      VVTELSNVV RMCTDKMSR YPRLREEAE RIITTHIRQ 
      REQYCKEQL CLLIDCELA YMNTNHEDF IGFANAQNQ 
      SENASKTGS RGTLGNQVI RKGYMCIHN LGIMKGGSR 
      DYWFVLTSE NISWYKDEE EREKKYMLP LDGLKLRDI 
      EQGFMSRRH MFALFNPDG RNVYKDYKQ LELSCETLD 
      EVDSWKASF LRAGVYPEK QTEQVNGEE AGGENVSSS 
      MDPQLERQV ETIRNLVDS YMRIVTKTT RDLVPKTIM 
      YMIINHTKD FINGELLAH IYASGDQSQ MMEEAPEEA 
      QKREEMLRM YHACKEALH IIGDVSMAT VSTPVPPPV 
      KNDWLASGL ENPRLSPPS PGGPRKTAP NMGQVGSTG 
      SLGSRAPPP PPSSGRPAP AIPNRPGGG APPMPPGRP 
      GGQALPAPL VPTRIGGQG PQGGVQVPP QVQMAVGRA 
      VTNAAINEL SNAFKFHNR PVPNIPPRI PDRPNPGRP 
      N
    CDS sequence
    • >TC011058 RA
      atggcagga aactccgga atggagcag cttattccc 
      atcgtgaat aagctgcaa gacgctttc acccagttg 
      ggggtgcac atgagcctg gatctgccc caaatcgct 
      gttgtgggc ggacagtcg gctggaaaa agctccgtt 
      ttggagaat ttcgtcgga aaagacttc cttccgaga 
      ggatccggg attgtcacc agaaggccc cttatccta 
      caattgatt aactccaat tcggaatat gctgaattt 
      ctccactgt aaaggcaaa aaattcgtc gatttcgac 
      gaagtgcgt cgcgagatt gaagcggaa acggaccga 
      gtcaccgga agtaacaaa ggaatatcg aatattccc 
      atcaacttg agggtgtac tcaccccat gtgttaaat 
      ttaaccctg atcgatcta ccgggcttg acaaaagtg 
      cccattggc gatcaaccc ctcgacatc gaacaacaa 
      atcaaaggg atgataatg cagttcatc aaacgtgaa 
      tcgtgtctc atcttggcc gtaactccc gccaacacc 
      gatttggcc aactcggat gccctcaaa ctggccaaa 
      gaggtcgat cctcaaggt cttcggacg attggtgtt 
      atcacgaag cttgatttg atggatgaa gggaccgac 
      gctcgggac gtgctagaa aataagctt ttgcccttg 
      agaaggggt tatataggt gtggtgaat cgctcacag 
      aaggatatc gacgggcgg aaggatatc aaggcggcg 
      atggcggcc gaaaggcaa ttctttttg tcgcatccc 
      tcgtataga catcttgcc gataggttg ggaacaccc 
      tatttgcaa agggtgttg aatcaacaa ttgaccaac 
      cacataagg gatacgtta ccaggattg agggataaa 
      ttgcagaag cagttgttg acgcttgag aaggatgtc 
      gagcagttt aagcacttc aggcccgat gatcctgcg 
      attaaaacc aaggcgatg ttacagatg atccaacaa 
      ctccaaacc gacttcgaa cgcacgatc gaaggttcc 
      ggttcggct caaatcaac acaaatgaa ttatcaggt 
      ggtgcaaaa atcaatcgt ttattccac gagcgtttt 
      cccttcgaa atcgtcaaa atggaattt gatgaaaag 
      gaactgcga cgagaaatc gctttcgca attcgcaat 
      attcacggt atcagagtg gggcttttc acccccgat 
      atggcattc gaagcaatc gttaaaaaa caaattgct 
      cgattgaaa gaaccctca ctaaaatgc accgatcta 
      gtggtcact gaattgtca aatgttgtg cgcatgtgc 
      actgataag atgtcgcgc tatccgcga ctaagagag 
      gaggcagaa cgaatcatc accacacac atcagacag 
      agagagcaa tattgcaag gaacagttg tgcttactt 
      atcgactgc gaactggct tatatgaac acaaaccat 
      gaggatttt atcggtttt gccaatgcc caaaatcag 
      tccgagaat gcttctaag actgggtca cgtggcact 
      ttgggcaat caagtgatc cggaaaggc tacatgtgt 
      atccataat ttgggtata atgaaagga gggtcacgt 
      gattattgg ttcgttctc acgtctgaa aacatctca 
      tggtataag gatgaggaa gagagggag aaaaaatac 
      atgttgcca ttggatggc ttaaaactg agagacata 
      gaacagggt tttatgtca cgacgacac atgtttgcg 
      ttgtttaat cctgacggt cgtaacgtc tacaaggat 
      tacaaacaa ctcgaattg agttgtgag actttggat 
      gaagtcgat tcgtggaag gcgtcgttc ttgagggcg 
      ggggtttac ccggaaaag cagacggaa caagtgaac 
      ggcgaagag gccggtggt gagaacgtg agcagttca 
      atggacccc caactcgaa cgtcaagtg gaaacaatt 
      cgaaatttg gtcgacagt tacatgcga atcgtgaca 
      aaaacaacc cgagatcta gtaccaaaa acaataatg 
      tacatgatc ataaaccac accaaagac ttcataaac 
      ggcgaactt ttagcccac atttacgcc agtggtgat 
      cagtcccaa atgatggaa gaagctccc gaagaggcc 
      caaaaacgc gaagaaatg ctccgtatg taccacgcg 
      tgcaaggag gcgttgcac atcatcggt gacgtttcc 
      atggcgacg gtttccacg cccgtgccg cctccggtg 
      aaaaatgat tggttggcg agcggcctt gagaatcca 
      cgtttgtcg ccaccgagt ccaggcgga cccagaaaa 
      accgccccc aatatggga caagtcggc agcactggt 
      tcgttaggt tcgagggct ccgccgccg cccccaagc 
      tccgggaga ccggccccc gccattccg aaccgcccc 
      gggggcgga gcaccccca atgccccca ggcaggccg 
      ggaggacaa gccttgccg gcgcccctc gtccccact 
      cgaattggg ggccaagga ccgcaaggg ggcgtccaa 
      gtgcctcct caggtgcag atggcagtc ggacgcgct 
      gttaccaat gctgctatc aacgaactc tccaatgcc 
      ttcaagttc cataatcgg ccagttcca aacatacct 
      ccaagaata ccagacagg ccaaacccg ggacgacca 
      aattaa
    mRNA sequence
    • >TC011058 RA
      tccgaattc aaaaatccc attttatca gcttgagtt 
      tgacattta ttcttgaaa aaatcgttc ccttatcac 
      tgtaatcac tgttctatc gcccaattc tccccgaaa 
      cgtgtaatt ttgtgatat atctcgtcg tgaagtgta 
      aatggatga gtcatgcgc gttcagtta tgattcggc 
      catgttccc aagtgtata ttgttaaag tgtcgaagt 
      gaatttagt tgttttcga gggaaatta gcacagttc 
      cggcctaaa aatggcagg aaactccgg aatggagca 
      gcttattcc catcgtgaa taagctgca agacgcttt 
      cacccagtt gggggtgca catgagcct ggatctgcc 
      ccaaatcgc tgttgtggg cggacagtc ggctggaaa 
      aagctccgt tttggagaa tttcgtcgg aaaagactt 
      ccttccgag aggatccgg gattgtcac cagaaggcc 
      ccttatcct acaattgat taactccaa ttcggaata 
      tgctgaatt tctccactg taaaggcaa aaaattcgt 
      cgatttcga cgaagtgcg tcgcgagat tgaagcgga 
      aacggaccg agtcaccgg aagtaacaa aggaatatc 
      gaatattcc catcaactt gagggtgta ctcacccca 
      tgtgttaaa tttaaccct gatcgatct accgggctt 
      gacaaaagt gcccattgg cgatcaacc cctcgacat 
      cgaacaaca aatcaaagg gatgataat gcagttcat 
      caaacgtga atcgtgtct catcttggc cgtaactcc 
      cgccaacac cgatttggc caactcgga tgccctcaa 
      actggccaa agaggtcga tcctcaagg tcttcggac 
      gattggtgt tatcacgaa gcttgattt gatggatga 
      agggaccga cgctcggga cgtgctaga aaataagct 
      tttgccctt gagaagggg ttatatagg tgtggtgaa 
      tcgctcaca gaaggatat cgacgggcg gaaggatat 
      caaggcggc gatggcggc cgaaaggca attcttttt 
      gtcgcatcc ctcgtatag acatcttgc cgataggtt 
      gggaacacc ctatttgca aagggtgtt gaatcaaca 
      attgaccaa ccacataag ggatacgtt accaggatt 
      gagggataa attgcagaa gcagttgtt gacgcttga 
      gaaggatgt cgagcagtt taagcactt caggcccga 
      tgatcctgc gattaaaac caaggcgat gttacagat 
      gatccaaca actccaaac cgacttcga acgcacgat 
      cgaaggttc cggttcggc tcaaatcaa cacaaatga 
      attatcagg tggtgcaaa aatcaatcg tttattcca 
      cgagcgttt tcccttcga aatcgtcaa aatggaatt 
      tgatgaaaa ggaactgcg acgagaaat cgctttcgc 
      aattcgcaa tattcacgg tatcagagt ggggctttt 
      cacccccga tatggcatt cgaagcaat cgttaaaaa 
      acaaattgc tcgattgaa agaaccctc actaaaatg 
      caccgatct agtggtcac tgaattgtc aaatgttgt 
      gcgcatgtg cactgataa gatgtcgcg ctatccgcg 
      actaagaga ggaggcaga acgaatcat caccacaca 
      catcagaca gagagagca atattgcaa ggaacagtt 
      gtgcttact tatcgactg cgaactggc ttatatgaa 
      cacaaacca tgaggattt tatcggttt tgccaatgc 
      ccaaaatca gtccgagaa tgcttctaa gactgggtc 
      acgtggcac tttgggcaa tcaagtgat ccggaaagg 
      ctacatgtg tatccataa tttgggtat aatgaaagg 
      agggtcacg tgattattg gttcgttct cacgtctga 
      aaacatctc atggtataa ggatgagga agagaggga 
      gaaaaaata catgttgcc attggatgg cttaaaact 
      gagagacat agaacaggg ttttatgtc acgacgaca 
      catgtttgc gttgtttaa tcctgacgg tcgtaacgt 
      ctacaagga ttacaaaca actcgaatt gagttgtga 
      gactttgga tgaagtcga ttcgtggaa ggcgtcgtt 
      cttgagggc gggggttta cccggaaaa gcagacgga 
      acaagtgaa cggcgaaga ggccggtgg tgagaacgt 
      gagcagttc aatggaccc ccaactcga acgtcaagt 
      ggaaacaat tcgaaattt ggtcgacag ttacatgcg 
      aatcgtgac aaaaacaac ccgagatct agtaccaaa 
      aacaataat gtacatgat cataaacca caccaaaga 
      cttcataaa cggcgaact tttagccca catttacgc 
      cagtggtga tcagtccca aatgatgga agaagctcc 
      cgaagaggc ccaaaaacg cgaagaaat gctccgtat 
      gtaccacgc gtgcaagga ggcgttgca catcatcgg 
      tgacgtttc catggcgac ggtttccac gcccgtgcc 
      gcctccggt gaaaaatga ttggttggc gagcggcct 
      tgagaatcc acgtttgtc gccaccgag tccaggcgg 
      acccagaaa aaccgcccc caatatggg acaagtcgg 
      cagcactgg ttcgttagg ttcgagggc tccgccgcc 
      gcccccaag ctccgggag accggcccc cgccattcc 
      gaaccgccc cgggggcgg agcaccccc aatgccccc 
      aggcaggcc gggaggaca agccttgcc ggcgcccct 
      cgtccccac tcgaattgg gggccaagg accgcaagg 
      gggcgtcca agtgcctcc tcaggtgca gatggcagt 
      cggacgcgc tgttaccaa tgctgctat caacgaact 
      ctccaatgc cttcaagtt ccataatcg gccagttcc 
      aaacatacc tccaagaat accagacag gccaaaccc 
      gggacgacc aaattaagt tctagtcaa aattgaaac 
      gatgggacc aacaaggtt aatttagtg attgttaag 
      acttggcgc tttttcagt acaatgaca gtaatttgt 
      acctagtct aatgtatag tattacccc cagtcccgc 
      ttttgtaca tatctgtat tcaccattt gtaatatat 
      tgtttattt ttat