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Gene information
General information
TC011057 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid75: 1096436..1103226
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011057
    iBeetle screen iB_01792
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011057 RA
      MGLLLYTCV LMALLTLLY RISSVARYY LKYVFFGLS 
      SCFLASAPI PLMLRKPRD PKNALIPAW GIRMACRLI 
      GVTTSLEGH ENIVKDSGC VILMNHQSM LDLLILAEL 
      WPAMDNCTV ISKKEILYF QPFGLAAWL WGTIFIDRV 
      KKEHAQAAV NKTGETIRT RKARVLMFP EGTRNSKET 
      LLPFKKGAF HVALASKCP IQPVVVNRY RFLGKHRFD 
      DGHLRIKIL PAISTEGYT VENMQELMD KTYKIMAHE 
      VDVLTKEVD AGKMKKE
    CDS sequence
    • >TC011057 RA
      atgggcctc cttttgtac acttgtgtc ctaatggcc 
      ctcctcacc ctactttac aggataagt tcagtggcc 
      cgttattac ctaaaatat gtatttttc ggactgtct 
      agttgtttc ctggcttcg gcccccata cctctcatg 
      ttgcgtaaa ccacgcgac cccaaaaac gccttgatt 
      ccagcctgg ggtattcga atggcgtgt cgtttaata 
      ggggtcact actagtctc gaaggtcac gaaaacatc 
      gtcaaggac tcgggttgt gtgattcta atgaaccac 
      caatcaatg ctcgattta ttaattttg gccgaactg 
      tggccggct atggacaac tgcaccgtc atttccaaa 
      aaggaaatt ttgtacttc cagcccttt gggctcgcg 
      gcctggctt tggggcacg attttcatc gacagagtg 
      aagaaagag cacgctcaa gcggccgtt aacaaaacc 
      ggagagacg ataaggact cgcaaagcg agagtactt 
      atgttcccc gaagggaca aggaacagt aaagagacg 
      ctcctgccc ttcaaaaag ggggctttc cacgtggct 
      ttggcctcg aagtgtccc atccagccc gtggtggtc 
      aatcggtac aggtttttg gggaagcac cggtttgac 
      gacgggcat ttgaggatc aagattttg cctgctatt 
      tccacagaa gggtacacg gtggaaaat atgcaagaa 
      ttgatggat aaaacttac aaaattatg gcacacgaa 
      gtcgatgtt ttgaccaaa gaagtggac gcagggaag 
      atgaaaaaa gaatag
    mRNA sequence
    • >TC011057 RA
      acaaagata actcgattt ccagtgcga taagcgccc 
      gcttgtcaa tttttcttc atgaatcat ttggtcgtc 
      aggtgacta aatcgggtc aaagttaga cacaacatt 
      gcactgaaa ataatttgt aattgcaag gccattcca 
      tatcgcgtg aatttgaaa aatcacgaa ttccaagtc 
      atgcaatca ttaaattcg gcttttctt caattttga 
      taacaacat aatcactcc aaggccgca tggcgacca 
      ctcttccgc gaaacttcg aattcgaat cgttgcaaa 
      atgacgatt agttgactt ttattatca caacaagtg 
      cggtgtcgt catgtttcg ctcatcaca ccattaaaa 
      ttcgccatt tgacatcca atagccaaa ggtgtgcga 
      gttggcgcg agtttgtta tgaattgaa tttttgtga 
      ccacgtttc ggattttcg gtgataaaa atgggcctc 
      cttttgtac acttgtgtc ctaatggcc ctcctcacc 
      ctactttac aggataagt tcagtggcc cgttattac 
      ctaaaatat gtatttttc ggactgtct agttgtttc 
      ctggcttcg gcccccata cctctcatg ttgcgtaaa 
      ccacgcgac cccaaaaac gccttgatt ccagcctgg 
      ggtattcga atggcgtgt cgtttaata ggggtcact 
      actagtctc gaaggtcac gaaaacatc gtcaaggac 
      tcgggttgt gtgattcta atgaaccac caatcaatg 
      ctcgattta ttaattttg gccgaactg tggccggct 
      atggacaac tgcaccgtc atttccaaa aaggaaatt 
      ttgtacttc cagcccttt gggctcgcg gcctggctt 
      tggggcacg attttcatc gacagagtg aagaaagag 
      cacgctcaa gcggccgtt aacaaaacc ggagagacg 
      ataaggact cgcaaagcg agagtactt atgttcccc 
      gaagggaca aggaacagt aaagagacg ctcctgccc 
      ttcaaaaag ggggctttc cacgtggct ttggcctcg 
      aagtgtccc atccagccc gtggtggtc aatcggtac 
      aggtttttg gggaagcac cggtttgac gacgggcat 
      ttgaggatc aagattttg cctgctatt tccacagaa 
      gggtacacg gtggaaaat atgcaagaa ttgatggat 
      aaaacttac aaaattatg gcacacgaa gtcgatgtt 
      ttgaccaaa gaagtggac gcagggaag atgaaaaaa 
      gaataggga tttatacac ttgtgtaca tactttttt 
      gtaatttat tattatctt aattaaata attaaattt 
      tcttttaat tt