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Gene information
General information
TC011052 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5178420..5188450
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011052
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01791

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    labrum shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    gena triplet pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen complete anteriorconstricted - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    Adult morphology
    elytra posterior necrotic - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011052 RA
      MDLVLVLVL GLLVHAGGA TDPEIEDEE LRARDFLQF 
      LNKKNEESS NRLVLAEWA YASNITEQN LQNKLAVSA 
      EVAKEHKKD WEEVTKFNW NTFSDPDLK RQFKKYSKL 
      GSDALPEAK LNRYQKIIS EMEKIYSTA KICDFHNSS 
      KCDLSLEPH LTQILATSR NEEELRHVW VEWRNAVGP 
      HCKVLFEEY VDLANEAAR LNNFTNNAE NWLDNYEDA 
      QFRGQIEGL WEQLKPLYL QIHAYVRFK LREKYGEVV 
      SEKGPIPAH LLGNMWAQK WRTVSEFTL PYPDKKDED 
      ITSELKKQN YTAVKIFQT AEDFFKSIN LTEMPETFW 
      ERSILTKPN DREIVCHAS AWDFYDRKD FRIKQCTQI 
      TLEHFITAH HEMGHVEYF IQYKNQPIP FREGANSGF 
      HEAVGDLIA LSSEKDDPE NVLNNLFQI GLDKIVFLP 
      FGYLMDLWR WDVFEGKIT PDEYNCKWW ELREKYQGV 
      EPPLNRSEG DFDPAAKYH IVASVPYIR YFVSFVVQF 
      QFHKALCEK AGQYDPNDP SKPLHECDI YQSTEAGNA 
      LKKMLQLGS SKPWHEAME LLTGHKTMD ASAILEYFK 
      PLQKWLENE NQKNGAFIG WEKSTKVCV NSQKGEKPK 
      SK
    CDS sequence
    • >TC011052 RA
      atggatttg gtcttggtt ttggttttg gggcttttg 
      gtgcatgct ggaggtgca actgatcca gaaattgaa 
      gatgaagag ttgcgagct agggatttt cttcaattt 
      ttgaacaag aagaatgaa gaaagcagt aatcggctt 
      gtgctggca gaatgggcc tatgcttca aacatcacg 
      gagcaaaat ctccaaaat aagctagct gtgagcgcc 
      gaagtggcc aaagaacac aaaaaagac tgggaagaa 
      gtcaccaaa ttcaactgg aacaccttc tcagaccca 
      gacctcaaa cgccaattt aagaaatac tcaaaactg 
      ggctcggac gcactcccg gaggccaaa ctcaaccgc 
      taccagaaa atcatcagc gagatggaa aaaatctac 
      tcaactgcg aaaatctgc gactttcac aactcctca 
      aagtgcgat ctttcgctc gaaccgcac ttgactcaa 
      attttggcg acttcgcgc aatgaggag gagttgagg 
      cacgtttgg gttgagtgg aggaacgct gtggggccg 
      cactgtaag gtgcttttt gaggagtat gtcgatttg 
      gccaatgag gcggcaagg cttaataat tttacgaat 
      aatgcggag aattggttg gataattac gaggatgcg 
      cagtttaga ggacagatt gaggggctg tgggagcag 
      ctgaagccg ctctatttg caaatacac gcctatgtg 
      aggttcaag ttgagggag aagtatggc gaagttgtg 
      tcggagaaa gggccaatt ccggcgcat ctcttaggc 
      aacatgtgg gctcaaaaa tggagaact gtatccgaa 
      ttcaccctt ccttaccct gacaaaaaa gatgaagat 
      attacatcc gaactaaaa aaacagaac tacaccgcc 
      gtaaaaatc ttccaaacc gctgaagac ttcttcaaa 
      tcaattaac ttaaccgaa atgcctgaa actttctgg 
      gagagatca attctaacc aaaccaaac gaccgcgaa 
      atcgtctgt catgcctca gcttgggac ttctacgac 
      cgcaaagac ttcaggatc aaacagtgc acccaaatc 
      accctggaa cacttcatc acagcccac cacgaaatg 
      ggacacgtt gaatacttc atccagtac aaaaaccaa 
      cccattccc ttcagagag ggtgctaac agcggattt 
      catgaagcc gtgggcgat ttgatcgca ctttcaagc 
      gaaaaggac gatcctgaa aacgtgtta aataaccta 
      tttcagatc ggacttgat aaaattgtc ttccttcct 
      tttggttat ttgatggat ttgtggaga tgggacgtt 
      ttcgagggg aaaatcacc ccagatgag tacaattgc 
      aagtggtgg gagctgagg gagaagtac caaggggtg 
      gaaccgccc cttaatcgc tcagagggt gactttgac 
      ccagctgcc aagtaccac atcgttgcc agcgtgccc 
      tatattaga tattttgta agctttgtg gtccagttc 
      cagttccat aaggccctt tgtgaaaaa gcgggtcaa 
      tatgacccc aatgacccc tcgaagccc ttgcacgag 
      tgtgatatt tatcaaagt actgaagct ggaaatgcc 
      ctcaaaaaa atgcttcag ttaggttcg tccaagcca 
      tggcacgag gcgatggaa cttttgaca ggtcataaa 
      acaatggac gctagcgcc atcttggaa tattttaag 
      ccgttgcag aaatggttg gaaaatgaa aatcagaaa 
      aatggagct tttattgga tgggaaaag tctacgaaa 
      gtttgtgtc aatagtcaa aaaggggaa aaaccgaag 
      tcgaaatga
    mRNA sequence
    • >TC011052 RA
      aaagaaccc cgatttgtt ttgttggaa tatttccaa 
      aactggtcc caaaccaaa tccaaggca aaagcaatt 
      cacactctc gcgaaaaaa tcacatttt cctgcactg 
      cgtttcgtt tcctcttcg tccttcgtc ttgcaacgc 
      tggctctcg cctcaattt ggctcaaat tatgaaacg 
      tcgatgcag tttgaaatt gtgcaaaga gtgaaatgt 
      gattagttt gttgttcag tgcggtcgt tgcccatct 
      aaccggtct tggacgctt gctgaataa aagagacac 
      aaacaccca ttaccgact tattccctc gaaaccacg 
      acgaaaacg atggatttg gtcttggtt ttggttttg 
      gggcttttg gtgcatgct ggaggtgca actgatcca 
      gaaattgaa gatgaagag ttgcgagct agggatttt 
      cttcaattt ttgaacaag aagaatgaa gaaagcagt 
      aatcggctt gtgctggca gaatgggcc tatgcttca 
      aacatcacg gagcaaaat ctccaaaat aagctagct 
      gtgagcgcc gaagtggcc aaagaacac aaaaaagac 
      tgggaagaa gtcaccaaa ttcaactgg aacaccttc 
      tcagaccca gacctcaaa cgccaattt aagaaatac 
      tcaaaactg ggctcggac gcactcccg gaggccaaa 
      ctcaaccgc taccagaaa atcatcagc gagatggaa 
      aaaatctac tcaactgcg aaaatctgc gactttcac 
      aactcctca aagtgcgat ctttcgctc gaaccgcac 
      ttgactcaa attttggcg acttcgcgc aatgaggag 
      gagttgagg cacgtttgg gttgagtgg aggaacgct 
      gtggggccg cactgtaag gtgcttttt gaggagtat 
      gtcgatttg gccaatgag gcggcaagg cttaataat 
      tttacgaat aatgcggag aattggttg gataattac 
      gaggatgcg cagtttaga ggacagatt gaggggctg 
      tgggagcag ctgaagccg ctctatttg caaatacac 
      gcctatgtg aggttcaag ttgagggag aagtatggc 
      gaagttgtg tcggagaaa gggccaatt ccggcgcat 
      ctcttaggc aacatgtgg gctcaaaaa tggagaact 
      gtatccgaa ttcaccctt ccttaccct gacaaaaaa 
      gatgaagat attacatcc gaactaaaa aaacagaac 
      tacaccgcc gtaaaaatc ttccaaacc gctgaagac 
      ttcttcaaa tcaattaac ttaaccgaa atgcctgaa 
      actttctgg gagagatca attctaacc aaaccaaac 
      gaccgcgaa atcgtctgt catgcctca gcttgggac 
      ttctacgac cgcaaagac ttcaggatc aaacagtgc 
      acccaaatc accctggaa cacttcatc acagcccac 
      cacgaaatg ggacacgtt gaatacttc atccagtac 
      aaaaaccaa cccattccc ttcagagag ggtgctaac 
      agcggattt catgaagcc gtgggcgat ttgatcgca 
      ctttcaagc gaaaaggac gatcctgaa aacgtgtta 
      aataaccta tttcagatc ggacttgat aaaattgtc 
      ttccttcct tttggttat ttgatggat ttgtggaga 
      tgggacgtt ttcgagggg aaaatcacc ccagatgag 
      tacaattgc aagtggtgg gagctgagg gagaagtac 
      caaggggtg gaaccgccc cttaatcgc tcagagggt 
      gactttgac ccagctgcc aagtaccac atcgttgcc 
      agcgtgccc tatattaga tattttgta agctttgtg 
      gtccagttc cagttccat aaggccctt tgtgaaaaa 
      gcgggtcaa tatgacccc aatgacccc tcgaagccc 
      ttgcacgag tgtgatatt tatcaaagt actgaagct 
      ggaaatgcc ctcaaaaaa atgcttcag ttaggttcg 
      tccaagcca tggcacgag gcgatggaa cttttgaca 
      ggtcataaa acaatggac gctagcgcc atcttggaa 
      tattttaag ccgttgcag aaatggttg gaaaatgaa 
      aatcagaaa aatggagct tttattgga tgggaaaag 
      tctacgaaa gtttgtgtc aatagtcaa aaaggggaa 
      aaaccgaag tcgaaatga agttatttg agcttggac 
      ttgcagatt ttttagatg taaacatat taaaataaa 
      attgtaaaa cttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY