Status
Please wait ...
Gene information
General information
TC011049 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5040608..5053465
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011049
    iBeetle screen iB_01788

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011049 RA
      MKSQTEYKV KSPPEDAIS SVKFGPNTN QFLLVSSWD 
      GTVRLYDVT TNNLRHKYA HDAPVLDCC FTDAVHSYS 
      GGLDNTLKS FDFNTTTEN TVGAHANAI KCVEYCSEV 
      NGILTGSWD HHIKLWDPR TPLCSGSYN QGEKVYTIS 
      VCGEKLVVG TAGRKILVW DIRNMSYTL QKRESNLKY 
      QTRAIRCFP NKQGFVLSS IEGRVAVEY LDTNPEIQK 
      KKYAFKCHR IKEDGMEKI YPVNAISFH PTHNTFATG 
      GSDGYVNIW DGFNKKRLC QFHQYHTSI TSLSFSHNG 
      SVLAIACSY FLEEENPPN PLPEDAIYI RAVTDQETK 
      PKYSSNS
    CDS sequence
    • >TC011049 RA
      atgaaatct cagaccgag tacaaggta aaaagtccg 
      ccagaagac gcaatttcg tcggtgaaa tttggcccc 
      aataccaat cagtttctg ttggtcagc tcttgggat 
      ggtactgtg cggctatat gatgttact acgaataat 
      ttgaggcat aaatatgcc catgatgct ccagttttg 
      gattgctgt tttactgac gccgtccac tcgtacagc 
      ggcggcctg gacaacacc ctaaagtcc ttcgacttc 
      aacacaacc accgaaaac accgtcggc gctcacgca 
      aatgccatc aaatgcgtg gaatattgc agtgaagtg 
      aatggaatt ttaacagga agttgggac caccacatc 
      aaactatgg gacccacgc acgccactg tgtagcggg 
      agctacaac caaggcgaa aaagtctac acaataagc 
      gtctgtggc gagaaactg gtcgttgga acagccggc 
      cgaaaaatc ctagtgtgg gatattaga aatatgtca 
      tacacttta caaaaacgc gaatctaat ttgaaatat 
      cagacgagg gcgatcagg tgtttccct aataagcag 
      gggtttgtt ttgagtagt attgagggg agggtggcg 
      gttgaatat ctagacacc aatcctgag attcagaag 
      aaaaaatat gccttcaag tgtcataga attaaggag 
      gatggcatg gaaaaaatt tatccagtg aatgcgatc 
      agttttcac ccaacacat aacacgttc gcaaccggt 
      ggttccgac ggttatgtg aacatttgg gacggtttt 
      aataaaaaa cgcttgtgt cagttccac caatatcat 
      acatcaatt acgtcactt agttttagt cacaatggt 
      tctgtgttg gccattgca tgttcctac tttttggag 
      gaggaaaat cccccaaat ccgctaccg gaagacgcc 
      atctatatt cgagctgtc acagaccag gaaaccaaa 
      ccgaaatat agcagcaat tcataa
    mRNA sequence
    • >TC011049 RA
      acaatattt aggttatgt tagtgacag ttaacctct 
      caccaaacg ttatatttt tattaatag ccggtaaag 
      ttgataaat atttggcaa aattgctga aaacatgaa 
      atctcagac cgagtacaa ggtaaaaag tccgccaga 
      agacgcaat ttcgtcggt gaaatttgg ccccaatac 
      caatcagtt tctgttggt cagctcttg ggatggtac 
      tgtgcggct atatgatgt tactacgaa taatttgag 
      gcataaata tgcccatga tgctccagt tttggattg 
      ctgttttac tgacgccgt ccactcgta cagcggcgg 
      cctggacaa caccctaaa gtccttcga cttcaacac 
      aaccaccga aaacaccgt cggcgctca cgcaaatgc 
      catcaaatg cgtggaata ttgcagtga agtgaatgg 
      aattttaac aggaagttg ggaccacca catcaaact 
      atgggaccc acgcacgcc actgtgtag cgggagcta 
      caaccaagg cgaaaaagt ctacacaat aagcgtctg 
      tggcgagaa actggtcgt tggaacagc cggccgaaa 
      aatcctagt gtgggatat tagaaatat gtcatacac 
      tttacaaaa acgcgaatc taatttgaa atatcagac 
      gagggcgat caggtgttt ccctaataa gcaggggtt 
      tgttttgag tagtattga ggggagggt ggcggttga 
      atatctaga caccaatcc tgagattca gaagaaaaa 
      atatgcctt caagtgtca tagaattaa ggaggatgg 
      catggaaaa aatttatcc agtgaatgc gatcagttt 
      tcacccaac acataacac gttcgcaac cggtggttc 
      cgacggtta tgtgaacat ttgggacgg ttttaataa 
      aaaacgctt gtgtcagtt ccaccaata tcatacatc 
      aattacgtc acttagttt tagtcacaa tggttctgt 
      gttggccat tgcatgttc ctacttttt ggaggagga 
      aaatccccc aaatccgct accggaaga cgccatcta 
      tattcgagc tgtcacaga ccaggaaac caaaccgaa 
      atatagcag caattcata attttttgt attaataaa 
      attta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY