| General information | |||
| TC011043 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 6184584..6190575 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011043 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01787 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
- >TC011043 RA
MFLKSCDTC LAEFRKAYI KGACLQCDN VVRGANSPS KDFCSTYCM NKYQKKAEL QNRVAAVAP TTPLEASPG PFQYETYQP FDWDEYLKE TNSVAAPAS CFKQAVTPP HNEFKVSMK LEALDPRNV TSTCIATVI TVLGSRLRL RLDGSDNKN DFWRLVDSK EINPVGHCE KSGGMLQPP LGFRMNASS WPMFLTKTL NGALMAPAN IFQKEPPSP KCNLFQVGQ KLEAVDKKN PQLICCATV GAVKNEQIH VTFDGWRGA FDYWCRYDS RDIFPVGWC AKSGHPMQP PGQKNSTGH ARYKFKPAL TAPIPPISS TVAESPEAD TSAPPPPPI TIQVRKSCM GGPLIDATQ LPLTIEETT SKALAKSLI GKILVASHK SETILARLG ALRGEEMVI THQSTTYTI KLPQLSKAT DLENVFTAL ASALGCCHN LFSLDGMKK ECSSCDVKP SPPKRKPPP EQEAATSTT PGDTSASES PLASLNKIP TQEWGIEEV IQFIESADS CLGVHADLF RKHEIDGKA LLLLNSDMM MKYMGLKLG PALKICNLV SRLKGRRYN SL
- >TC011043 RA
atgtttttg aaatcgtgc gatacatgt ctcgccgag ttccgcaag gcatacatc aagggggcc tgcctccag tgcgacaac gtggtccgg ggcgccaac agcccctcg aaggacttc tgctcgacg tactgcatg aacaagtac cagaagaag gccgagttg cagaatcgc gtggctgcg gtcgccccc acgacgccg ctcgaggcc tcgccgggc cccttccag tacgagacg tatcagccg ttcgactgg gacgagtat ctcaaagag acgaactcg gtggcggcg cccgcgagc tgcttcaag caggcggtg acgccgcca cataacgag ttcaaagtg tcgatgaag ttggaggcg ttggatccc aggaatgtc acctccacg tgcattgcc acggtgatc acggttcta ggatccagg ctcaggttg aggctggat gggagtgat aataagaat gatttttgg agactggtt gactcgaag gagattaat cccgtgggg cattgtgag aagtcaggg gggatgcta cagccccca ttaggtttt cgcatgaac gcgagttcg tggccgatg ttcctcacg aaaacgctc aacggtgcc ctaatggcc cccgccaac atattccaa aaagagccc ccaagtccc aaatgcaac ctattccaa gttgggcaa aaactcgaa gctgttgat aagaaaaac ccccaattg atatgttgt gctacagtc ggtgcagta aaaaacgaa cagattcat gttacgttc gacggctgg agaggtgct ttcgactat tggtgccgt tacgacagc agagacatt tttccggtc ggatggtgc gccaaatcg gggcatccg atgcagccc ccgggacag aaaaacagc accggacat gccagatat aagtttaag ccggctttg acagccccc ataccgccg atttcgtcc acggtggcg gagtcgccc gaagcggac acttcggcg cccccgccg cccccaatc accattcag gtgcggaaa agctgcatg gggggcccc ctgatcgac gccacccag ctgccgctg acaatcgaa gaaacgaca tcaaaagcc ctagccaag agcctcatc ggcaaaatt ttagtcgct tcacacaaa tctgagacg attctagcc cgactgggg gcgttacga ggcgaagaa atggtcatc acccaccaa agcaccacc tacaccata aaattaccc caattatcg aaagctacc gacttggaa aacgtcttc acagcgctg gcgtcggca ttggggtgt tgccataac cttttctca ttagacggt atgaagaaa gaatgttcc tcgtgtgat gttaaaccg tcaccaccg aaacgcaaa ccgccccca gagcaagaa gcagccact tcgacaacc cccggcgat acttcggcc tccgaatcg ccactcgct agtctcaat aaaatcccc acacaggag tgggggatt gaagaagtg atacagttt atcgagtcg gcggattcg tgtctagga gtccacgcc gacttgttc cgcaaacat gaaatcgac ggaaaggcg ctgctgctt ctcaattcg gacatgatg atgaagtac atgggtctg aagctagga ccggcactg aaaatatgt aatttggtc tcgcggctt aagggcaga aggtacaat tcgctttaa
- >TC011043 RA
gacgtcgtt gacattgtg atgtatttt tccgtttaa tcccggttt tgcgtcact accccactg caggcgccc ccagtggtc tttccgatg taatcggtg ccgtagacc gtgcgacag ttctccccc gccatgccc ccagcgccc gcctgattt tggaggtaa actgatgtt tttgaaatc gtgcgatac atgtctcgc cgagttccg caaggcata catcaaggg ggcctgcct ccagtgcga caacgtggt ccggggcgc caacagccc ctcgaagga cttctgctc gacgtactg catgaacaa gtaccagaa gaaggccga gttgcagaa tcgcgtggc tgcggtcgc ccccacgac gccgctcga ggcctcgcc gggcccctt ccagtacga gacgtatca gccgttcga ctgggacga gtatctcaa agagacgaa ctcggtggc ggcgcccgc gagctgctt caagcaggc ggtgacgcc gccacataa cgagttcaa agtgtcgat gaagttgga ggcgttgga tcccaggaa tgtcacctc cacgtgcat tgccacggt gatcacggt tctaggatc caggctcag gttgaggct ggatgggag tgataataa gaatgattt ttggagact ggttgactc gaaggagat taatcccgt ggggcattg tgagaagtc aggggggat gctacagcc cccattagg ttttcgcat gaacgcgag ttcgtggcc gatgttcct cacgaaaac gctcaacgg tgccctaat ggcccccgc caacatatt ccaaaaaga gcccccaag tcccaaatg caacctatt ccaagttgg gcaaaaact cgaagctgt tgataagaa aaaccccca attgatatg ttgtgctac agtcggtgc agtaaaaaa cgaacagat tcatgttac gttcgacgg ctggagagg tgctttcga ctattggtg ccgttacga cagcagaga catttttcc ggtcggatg gtgcgccaa atcggggca tccgatgca gcccccggg acagaaaaa cagcaccgg acatgccag atataagtt taagccggc tttgacagc ccccatacc gccgatttc gtccacggt ggcggagtc gcccgaagc ggacacttc ggcgccccc gccgccccc aatcaccat tcaggtgcg gaaaagctg catgggggg ccccctgat cgacgccac ccagctgcc gctgacaat cgaagaaac gacatcaaa agccctagc caagagcct catcggcaa aattttagt cgcttcaca caaatctga gacgattct agcccgact gggggcgtt acgaggcga agaaatggt catcaccca ccaaagcac cacctacac cataaaatt accccaatt atcgaaagc taccgactt ggaaaacgt cttcacagc gctggcgtc ggcattggg gtgttgcca taacctttt ctcattaga cggtatgaa gaaagaatg ttcctcgtg tgatgttaa accgtcacc accgaaacg caaaccgcc cccagagca agaagcagc cacttcgac aacccccgg cgatacttc ggcctccga atcgccact cgctagtct caataaaat ccccacaca ggagtgggg gattgaaga agtgataca gtttatcga gtcggcgga ttcgtgtct aggagtcca cgccgactt gttccgcaa acatgaaat cgacggaaa ggcgctgct gcttctcaa ttcggacat gatgatgaa gtacatggg tctgaagct aggaccggc actgaaaat atgtaattt ggtctcgcg gcttaaggg cagaaggta caattcgct ttaaggaag aaaagaaag tctattttt ccgtacttg gatgcataa attgtgtca ttgttatgt acctccctt agagttata tatttttta ttatcagaa aacattttt ttcctaatg tttgtgata cgttcaagt ctcgtttat cggcaatac aattgtatc gtaaattgt aggtttagc gactcgatt ctccgttcg tttattcca aattgcgca ttttccagt atttcgtta ggagctttt ttgtagaaa taaataaaa gtatttaat tttcttacc attttgatt tcattttga aaaaatgaa acgtgcatt tagcttagc tcttttata gacgacgca gtggatgtt tggcatggg gacactgct gtttgtttc aagcatttt taccgatta gtttaagtg ttgagattg tttaaattt aattctgta acagtgagt taataaaga ggaatatca aac
