Status
Please wait ...
Gene information
General information
TC011043 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6184584..6190575
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011043
    iBeetle screen iB_01787

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011043 RA
      MFLKSCDTC LAEFRKAYI KGACLQCDN VVRGANSPS 
      KDFCSTYCM NKYQKKAEL QNRVAAVAP TTPLEASPG 
      PFQYETYQP FDWDEYLKE TNSVAAPAS CFKQAVTPP 
      HNEFKVSMK LEALDPRNV TSTCIATVI TVLGSRLRL 
      RLDGSDNKN DFWRLVDSK EINPVGHCE KSGGMLQPP 
      LGFRMNASS WPMFLTKTL NGALMAPAN IFQKEPPSP 
      KCNLFQVGQ KLEAVDKKN PQLICCATV GAVKNEQIH 
      VTFDGWRGA FDYWCRYDS RDIFPVGWC AKSGHPMQP 
      PGQKNSTGH ARYKFKPAL TAPIPPISS TVAESPEAD 
      TSAPPPPPI TIQVRKSCM GGPLIDATQ LPLTIEETT 
      SKALAKSLI GKILVASHK SETILARLG ALRGEEMVI 
      THQSTTYTI KLPQLSKAT DLENVFTAL ASALGCCHN 
      LFSLDGMKK ECSSCDVKP SPPKRKPPP EQEAATSTT 
      PGDTSASES PLASLNKIP TQEWGIEEV IQFIESADS 
      CLGVHADLF RKHEIDGKA LLLLNSDMM MKYMGLKLG 
      PALKICNLV SRLKGRRYN SL
    CDS sequence
    • >TC011043 RA
      atgtttttg aaatcgtgc gatacatgt ctcgccgag 
      ttccgcaag gcatacatc aagggggcc tgcctccag 
      tgcgacaac gtggtccgg ggcgccaac agcccctcg 
      aaggacttc tgctcgacg tactgcatg aacaagtac 
      cagaagaag gccgagttg cagaatcgc gtggctgcg 
      gtcgccccc acgacgccg ctcgaggcc tcgccgggc 
      cccttccag tacgagacg tatcagccg ttcgactgg 
      gacgagtat ctcaaagag acgaactcg gtggcggcg 
      cccgcgagc tgcttcaag caggcggtg acgccgcca 
      cataacgag ttcaaagtg tcgatgaag ttggaggcg 
      ttggatccc aggaatgtc acctccacg tgcattgcc 
      acggtgatc acggttcta ggatccagg ctcaggttg 
      aggctggat gggagtgat aataagaat gatttttgg 
      agactggtt gactcgaag gagattaat cccgtgggg 
      cattgtgag aagtcaggg gggatgcta cagccccca 
      ttaggtttt cgcatgaac gcgagttcg tggccgatg 
      ttcctcacg aaaacgctc aacggtgcc ctaatggcc 
      cccgccaac atattccaa aaagagccc ccaagtccc 
      aaatgcaac ctattccaa gttgggcaa aaactcgaa 
      gctgttgat aagaaaaac ccccaattg atatgttgt 
      gctacagtc ggtgcagta aaaaacgaa cagattcat 
      gttacgttc gacggctgg agaggtgct ttcgactat 
      tggtgccgt tacgacagc agagacatt tttccggtc 
      ggatggtgc gccaaatcg gggcatccg atgcagccc 
      ccgggacag aaaaacagc accggacat gccagatat 
      aagtttaag ccggctttg acagccccc ataccgccg 
      atttcgtcc acggtggcg gagtcgccc gaagcggac 
      acttcggcg cccccgccg cccccaatc accattcag 
      gtgcggaaa agctgcatg gggggcccc ctgatcgac 
      gccacccag ctgccgctg acaatcgaa gaaacgaca 
      tcaaaagcc ctagccaag agcctcatc ggcaaaatt 
      ttagtcgct tcacacaaa tctgagacg attctagcc 
      cgactgggg gcgttacga ggcgaagaa atggtcatc 
      acccaccaa agcaccacc tacaccata aaattaccc 
      caattatcg aaagctacc gacttggaa aacgtcttc 
      acagcgctg gcgtcggca ttggggtgt tgccataac 
      cttttctca ttagacggt atgaagaaa gaatgttcc 
      tcgtgtgat gttaaaccg tcaccaccg aaacgcaaa 
      ccgccccca gagcaagaa gcagccact tcgacaacc 
      cccggcgat acttcggcc tccgaatcg ccactcgct 
      agtctcaat aaaatcccc acacaggag tgggggatt 
      gaagaagtg atacagttt atcgagtcg gcggattcg 
      tgtctagga gtccacgcc gacttgttc cgcaaacat 
      gaaatcgac ggaaaggcg ctgctgctt ctcaattcg 
      gacatgatg atgaagtac atgggtctg aagctagga 
      ccggcactg aaaatatgt aatttggtc tcgcggctt 
      aagggcaga aggtacaat tcgctttaa
    mRNA sequence
    • >TC011043 RA
      gacgtcgtt gacattgtg atgtatttt tccgtttaa 
      tcccggttt tgcgtcact accccactg caggcgccc 
      ccagtggtc tttccgatg taatcggtg ccgtagacc 
      gtgcgacag ttctccccc gccatgccc ccagcgccc 
      gcctgattt tggaggtaa actgatgtt tttgaaatc 
      gtgcgatac atgtctcgc cgagttccg caaggcata 
      catcaaggg ggcctgcct ccagtgcga caacgtggt 
      ccggggcgc caacagccc ctcgaagga cttctgctc 
      gacgtactg catgaacaa gtaccagaa gaaggccga 
      gttgcagaa tcgcgtggc tgcggtcgc ccccacgac 
      gccgctcga ggcctcgcc gggcccctt ccagtacga 
      gacgtatca gccgttcga ctgggacga gtatctcaa 
      agagacgaa ctcggtggc ggcgcccgc gagctgctt 
      caagcaggc ggtgacgcc gccacataa cgagttcaa 
      agtgtcgat gaagttgga ggcgttgga tcccaggaa 
      tgtcacctc cacgtgcat tgccacggt gatcacggt 
      tctaggatc caggctcag gttgaggct ggatgggag 
      tgataataa gaatgattt ttggagact ggttgactc 
      gaaggagat taatcccgt ggggcattg tgagaagtc 
      aggggggat gctacagcc cccattagg ttttcgcat 
      gaacgcgag ttcgtggcc gatgttcct cacgaaaac 
      gctcaacgg tgccctaat ggcccccgc caacatatt 
      ccaaaaaga gcccccaag tcccaaatg caacctatt 
      ccaagttgg gcaaaaact cgaagctgt tgataagaa 
      aaaccccca attgatatg ttgtgctac agtcggtgc 
      agtaaaaaa cgaacagat tcatgttac gttcgacgg 
      ctggagagg tgctttcga ctattggtg ccgttacga 
      cagcagaga catttttcc ggtcggatg gtgcgccaa 
      atcggggca tccgatgca gcccccggg acagaaaaa 
      cagcaccgg acatgccag atataagtt taagccggc 
      tttgacagc ccccatacc gccgatttc gtccacggt 
      ggcggagtc gcccgaagc ggacacttc ggcgccccc 
      gccgccccc aatcaccat tcaggtgcg gaaaagctg 
      catgggggg ccccctgat cgacgccac ccagctgcc 
      gctgacaat cgaagaaac gacatcaaa agccctagc 
      caagagcct catcggcaa aattttagt cgcttcaca 
      caaatctga gacgattct agcccgact gggggcgtt 
      acgaggcga agaaatggt catcaccca ccaaagcac 
      cacctacac cataaaatt accccaatt atcgaaagc 
      taccgactt ggaaaacgt cttcacagc gctggcgtc 
      ggcattggg gtgttgcca taacctttt ctcattaga 
      cggtatgaa gaaagaatg ttcctcgtg tgatgttaa 
      accgtcacc accgaaacg caaaccgcc cccagagca 
      agaagcagc cacttcgac aacccccgg cgatacttc 
      ggcctccga atcgccact cgctagtct caataaaat 
      ccccacaca ggagtgggg gattgaaga agtgataca 
      gtttatcga gtcggcgga ttcgtgtct aggagtcca 
      cgccgactt gttccgcaa acatgaaat cgacggaaa 
      ggcgctgct gcttctcaa ttcggacat gatgatgaa 
      gtacatggg tctgaagct aggaccggc actgaaaat 
      atgtaattt ggtctcgcg gcttaaggg cagaaggta 
      caattcgct ttaaggaag aaaagaaag tctattttt 
      ccgtacttg gatgcataa attgtgtca ttgttatgt 
      acctccctt agagttata tatttttta ttatcagaa 
      aacattttt ttcctaatg tttgtgata cgttcaagt 
      ctcgtttat cggcaatac aattgtatc gtaaattgt 
      aggtttagc gactcgatt ctccgttcg tttattcca 
      aattgcgca ttttccagt atttcgtta ggagctttt 
      ttgtagaaa taaataaaa gtatttaat tttcttacc 
      attttgatt tcattttga aaaaatgaa acgtgcatt 
      tagcttagc tcttttata gacgacgca gtggatgtt 
      tggcatggg gacactgct gtttgtttc aagcatttt 
      taccgatta gtttaagtg ttgagattg tttaaattt 
      aattctgta acagtgagt taataaaga ggaatatca 
      aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY