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Gene information
General information
TC011040 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid76: 23728..26377
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011040
    iBeetle screen iB_01786
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011040 RA
      MSIENEQNY DYSNIFVKT EKLSDTEIE CEEEIPDCD 
      IIKVEIKEE SPDDPSFLS PEEPPESEL IPTVKTEPE 
      SELKNFHCT LCPFKTKWK NSLRGHMKI HSSPEFVKL 
      YRCRKCAYK TKWKNYLAK HTRIHSAPD CLDMYKCTT 
      CSFQTKWKT SLKMHESIH SIKDTNLKI YKCTKCPFE 
      SNWKQYLNR HIKSHLPES LQKHKCPEC SFQTKWKQY 
      LKEHMKIHS APNTVKQYQ CEKCQFSTR WKQYFKEHV 
      KRHTSPKKT KSHKCTECI FEADSKVTL ARHLHKVHD 
      IVDKIEYFQ CSECMFKTR WKQYLKRHI KLHDETASY 
      KCPECPFET RWKNSLIGH QTVHSQAKP TALFHCTVC 
      PFQTKWKNS LAGHMAIHS PLDPSKMHQ CPSCPFQSR 
      SKTYLKRHI KIHTGEHEE TFSCPQCPF QTKWKQYFK 
      EHVKKHAPD QSPLFKCPE CEFTSKWKQ YFREHVKKH 
      QNPELLKQY ACEECDFTT TWRKCYREH VKQHSTPDK 
      VYKCTSCKF KTKWRQYFR EHVKKHQNP DLVKLYKCT 
      ICSFITIWK QYFKEHVER HNSTQIYKC AKCEYEGES 
      RYFLAKHVK EAHCQAEVK TYKCEKCGF TTKWKESIK 
      SHLRVHSNS KTAKIYKCA VCMFDTKWK SSFNSHMKM 
      VHSKPEVQK KYNTRRSKT GEECN
    CDS sequence
    • >TC011040 RA
      atgagcata gaaaatgaa caaaactac gactacagt 
      aacattttc gtgaagacg gagaagctc agcgatacg 
      gaaatcgag tgtgaggag gaaatcccc gattgtgat 
      ataatcaaa gtagaaata aaggaggaa tccccggac 
      gaccccagt tttttgagc cctgaggag ccccccgaa 
      agtgaatta atccccact gtgaaaact gagcccgaa 
      agtgagctc aagaacttc cactgcacg ctatgcccg 
      ttcaaaacg aaatggaaa aacagcctt cggggccac 
      atgaaaatc cacagctcg cccgaattc gtgaaactg 
      taccgttgc cgaaaatgc gcctacaaa accaaatgg 
      aaaaactat ctcgccaaa cacacgcgc attcacagc 
      gcccccgat tgcctggac atgtacaaa tgtacaacg 
      tgcagcttc cagaccaag tggaagacc agtctcaag 
      atgcacgag tcgattcat tcgatcaaa gacacgaac 
      ttgaagatt tacaagtgc accaagtgc ccgttcgag 
      tccaactgg aagcagtac ttgaaccgc cacatcaag 
      tcacacttg cccgagagc ttgcaaaag cacaaatgc 
      cccgagtgt agcttccaa accaaatgg aagcaatac 
      ttgaaggaa catatgaag atacactca gctcctaac 
      accgtcaag caataccag tgcgagaaa tgccagttt 
      agcacccgc tggaagcag tatttcaaa gagcacgtc 
      aagcggcat acttcgccc aaaaaaacc aaaagtcac 
      aaatgcacc gagtgtatc tttgaagcc gactcgaaa 
      gtcaccctc gcgcgccac ttgcacaaa gtccacgat 
      atcgtagac aaaatcgag tatttccag tgctccgag 
      tgcatgttt aagaccaga tggaagcag tacttgaag 
      cgccacatc aagctccac gacgagacc gctagttac 
      aagtgcccc gaatgcccg ttcgaaacc cggtggaag 
      aacagtctg atcggccac caaaccgtc cacagccag 
      gccaaaccc acggcactc ttccactgc accgtgtgc 
      cccttccag accaagtgg aagaacagt ctcgccggg 
      cacatggcc atccacagc cccctcgac ccgtcgaaa 
      atgcaccaa tgcccctca tgccccttc caatcccgc 
      tcgaaaacc tacctaaaa cgccacatc aaaatccac 
      accggcgag cacgaggag acgttctca tgcccccaa 
      tgccccttc caaaccaaa tggaagcag tacttcaaa 
      gagcacgtc aagaaacac gccccggac caaagcccg 
      ctcttcaaa tgcccggag tgcgagttc accagcaag 
      tggaagcag tacttccgg gagcacgtc aagaagcac 
      cagaacccg gagctgttg aagcagtac gcgtgcgag 
      gagtgcgac ttcaccacc acctggcgc aagtgctac 
      cgggagcat gtcaagcag cacagcacg cccgataag 
      gtgtacaag tgcaccagt tgcaagttc aagaccaag 
      tggaggcag tacttccgc gagcacgtc aagaagcac 
      caaaacccc gatctggtc aagctgtac aagtgcacg 
      atctgctcg tttatcacc atctggaag cagtatttt 
      aaggagcat gtcgagcga cacaactcc acgcagatt 
      tataagtgt gccaagtgc gagtacgag ggcgagtcg 
      aggtatttc ctggccaag cacgtgaag gaggcgcat 
      tgccaggcg gaggtgaag acgtacaag tgcgagaag 
      tgcggcttc acgaccaag tggaaggag agcatcaag 
      tcgcatctg agggtgcac tcgaattcg aaaactgcc 
      aagatttat aagtgtgcg gtttgtatg tttgatacc 
      aagtggaag agtagtttc aactcgcac atgaagatg 
      gtgcatagt aaaccagag gtgcagaag aagtataat 
      acgaggcgg agcaagact ggagaagag tgcaattga
    mRNA sequence
    • >TC011040 RA
      attatcaaa aaattggtc ccaagcaaa caagttatt 
      aattacgca ttatcgttg tgttagata aatgtttaa 
      acaatgtga agattgtgg cattcgaat gtggcggtc 
      ttttcggcc aatcaaaaa cgacagatg gcgctccgc 
      tttatttat tttttaata aacaagatt aataaaaaa 
      tcgtaaaaa aaacaccct gagtggttg tgttgcacg 
      aaaaaggga aatacgatg attaagtgg ccaaaaccg 
      tgtaaaagc aacaaaaac ctgtgacga aatcgtaaa 
      aatagtgtg ccgattgac agctaaaga aataaataa 
      aaatgagca tagaaaatg aacaaaact acgactaca 
      gtaacattt tcgtgaaga cggagaagc tcagcgata 
      cggaaatcg agtgtgagg aggaaatcc ccgattgtg 
      atataatca aagtagaaa taaaggagg aatccccgg 
      acgacccca gttttttga gccctgagg agccccccg 
      aaagtgaat taatcccca ctgtgaaaa ctgagcccg 
      aaagtgagc tcaagaact tccactgca cgctatgcc 
      cgttcaaaa cgaaatgga aaaacagcc ttcggggcc 
      acatgaaaa tccacagct cgcccgaat tcgtgaaac 
      tgtaccgtt gccgaaaat gcgcctaca aaaccaaat 
      ggaaaaact atctcgcca aacacacgc gcattcaca 
      gcgcccccg attgcctgg acatgtaca aatgtacaa 
      cgtgcagct tccagacca agtggaaga ccagtctca 
      agatgcacg agtcgattc attcgatca aagacacga 
      acttgaaga tttacaagt gcaccaagt gcccgttcg 
      agtccaact ggaagcagt acttgaacc gccacatca 
      agtcacact tgcccgaga gcttgcaaa agcacaaat 
      gccccgagt gtagcttcc aaaccaaat ggaagcaat 
      acttgaagg aacatatga agatacact cagctccta 
      acaccgtca agcaatacc agtgcgaga aatgccagt 
      ttagcaccc gctggaagc agtatttca aagagcacg 
      tcaagcggc atacttcgc ccaaaaaaa ccaaaagtc 
      acaaatgca ccgagtgta tctttgaag ccgactcga 
      aagtcaccc tcgcgcgcc acttgcaca aagtccacg 
      atatcgtag acaaaatcg agtatttcc agtgctccg 
      agtgcatgt ttaagacca gatggaagc agtacttga 
      agcgccaca tcaagctcc acgacgaga ccgctagtt 
      acaagtgcc ccgaatgcc cgttcgaaa cccggtgga 
      agaacagtc tgatcggcc accaaaccg tccacagcc 
      aggccaaac ccacggcac tcttccact gcaccgtgt 
      gccccttcc agaccaagt ggaagaaca gtctcgccg 
      ggcacatgg ccatccaca gccccctcg acccgtcga 
      aaatgcacc aatgcccct catgcccct tccaatccc 
      gctcgaaaa cctacctaa aacgccaca tcaaaatcc 
      acaccggcg agcacgagg agacgttct catgccccc 
      aatgcccct tccaaacca aatggaagc agtacttca 
      aagagcacg tcaagaaac acgccccgg accaaagcc 
      cgctcttca aatgcccgg agtgcgagt tcaccagca 
      agtggaagc agtacttcc gggagcacg tcaagaagc 
      accagaacc cggagctgt tgaagcagt acgcgtgcg 
      aggagtgcg acttcacca ccacctggc gcaagtgct 
      accgggagc atgtcaagc agcacagca cgcccgata 
      aggtgtaca agtgcacca gttgcaagt tcaagacca 
      agtggaggc agtacttcc gcgagcacg tcaagaagc 
      accaaaacc ccgatctgg tcaagctgt acaagtgca 
      cgatctgct cgtttatca ccatctgga agcagtatt 
      ttaaggagc atgtcgagc gacacaact ccacgcaga 
      tttataagt gtgccaagt gcgagtacg agggcgagt 
      cgaggtatt tcctggcca agcacgtga aggaggcgc 
      attgccagg cggaggtga agacgtaca agtgcgaga 
      agtgcggct tcacgacca agtggaagg agagcatca 
      agtcgcatc tgagggtgc actcgaatt cgaaaactg 
      ccaagattt ataagtgtg cggtttgta tgtttgata 
      ccaagtgga agagtagtt tcaactcgc acatgaaga 
      tggtgcata gtaaaccag aggtgcaga agaagtata 
      atacgaggc ggagcaaga ctggagaag agtgcaatt 
      gaaaggaaa gaaggctca tatgaatat aatgttgat 
      gtttttaaa attatattt aagaatctc tatgttagg 
      gtcatatcg ttcgtgtta atcttatat actctgctg 
      gcttgtaac ccaacacta ttaggagag aacaataat 
      tgctgtatc tacttttta ttttaaaga ttttattta 
      caatgtgac aatagaaat attcatttt aaaacgtgt 
      cttatttgc tgactacgg actattcgg aaataaaat 
      taactagac gcc