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Gene information
General information
TC011038 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6210201..6212098
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011038
    iBeetle screen iB_01785

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011038 RA
      MEYITELPP PEAPKNKIL CCECGTPIE PNPSNMCVP 
      CLRTKVDIT EGIPKQVTL QFCRGCERY YSPPSEWTH 
      CVLESKELL SLCLKKLKG LSRVKLVDA GFVWTEPHS 
      KRIKVKLRV HGEVLGGAV LEQVFIVDY VVSHHMCDD 
      CHRSEAQNF WRALVQVRQ KAENKKTFY YLEQLILKH 
      KAHDNTLGI KPIHDGLDF FYATEAHAR KMVDFLAAM 
      LPCKYNHSK KLISHDIHS NLYNYKFTY SVEIVPVSK 
      DSLVCLPKK LTQQLGGIS PICLVYRVT NSLHLIDPS 
      RAQIAEINS TVYWRSPFN AICNPKQLV EYVVMDIEP 
      IMDKDRKTF PGQGAISNK HVLSDIWVV RASELGVSD 
      NTIHTRTHL GHVLKPGDY VLGYNLEDS NINDGNFDK 
      LDKNSVPDV VLVKKYYDR SERKRRRVW KLKHMAQEE 
      TALITDNRD YNEFLDDLE EDPDLRQNI NIFKDNTKQ 
      IPIDSGDDV DENVPRITL AEMLDDLVI DDVEMSEV
    CDS sequence
    • >TC011038 RA
      atggaatat ataacggag ctgccgccc cccgaagcc 
      ccaaaaaac aaaattctg tgctgcgaa tgcggtacc 
      cccatcgag cccaacccc tcgaacatg tgcgtgcct 
      tgcctccgc accaaagtc gacatcact gaaggcatc 
      cccaaacag gtcactctg cagttctgt cgcggctgc 
      gagcgctac tacagcccc ccgtccgaa tggacccac 
      tgtgtgctc gaatcaaaa gaattactt tcattatgt 
      ttgaaaaaa ctgaaaggt ttgagtcgg gttaagttg 
      gtcgacgct ggctttgtc tggaccgaa ccccactcc 
      aaacgcatc aaagtcaag ctaagggtg cacggagaa 
      gtgctcggt ggggctgtg ctggagcag gtgtttatc 
      gttgattac gtggtcagt caccacatg tgcgatgac 
      tgtcaccgg tcggaagcc cagaacttc tggcgggct 
      ttggtgcaa gtgagacaa aaggccgag aataaaaaa 
      acgttctat tatctggag cagctgatt ttgaagcat 
      aaggcgcac gataacacc ctggggatt aaaccgatt 
      catgatggg cttgatttt ttctacgcc actgaggcc 
      cacgcccgg aaaatggtc gatttcctt gccgccatg 
      ctcccgtgc aaatacaat cattcgaaa aaactaatc 
      tcgcatgat attcacagc aacttgtac aattacaaa 
      ttcacatat tcggtggaa atcgttccg gtgtcgaaa 
      gacagtttg gtgtgtttg cccaaaaaa ctgacgcaa 
      caattgggc gggatttcc cccatttgt ctggtgtac 
      agggttacc aactcgctg catttgatc gacccatca 
      agggcgcaa attgccgaa ataaatagc actgtttat 
      tggcgctct ccgttcaat gccatctgt aatccgaaa 
      caactggtc gagtacgtg gttatggac attgagccg 
      attatggat aaagaccgt aagactttc ccgggacag 
      ggggctatt tcaaataag catgtcttg tcggatatt 
      tgggtcgtc agagcgtct gaattgggg gttagcgac 
      aatacaata cacacaagg acacacttg gggcacgtg 
      ttgaaacca ggcgactat gttttgggt tataatctt 
      gaagacagt aacataaat gatggaaat tttgataaa 
      ttggacaag aacagtgtc cctgatgtt gttttggtg 
      aagaaatat tacgatagg agtgagagg aagaggagg 
      agagtgtgg aagttgaaa cacatggct caggaagag 
      acggcgttg atcaccgat aacagggat tataacgag 
      tttttggac gatttggag gaagatccc gatttgcgc 
      caaaacatt aatattttt aaggataat acgaagcaa 
      attcccatc gattcgggt gatgatgtt gatgagaat 
      gttccaagg ataacattg gcggaaatg ttggacgat 
      ttggttatt gacgatgtt gaaatgtcc gaagtttga
    mRNA sequence
    • >TC011038 RA
      acatttgac agtggtcaa cttgtcaaa aatttgtgg 
      ttaggtgca agcacatgt gtcacaaat tccacccaa 
      aatggaata tataacgga gctgccgcc ccccgaagc 
      cccaaaaaa caaaattct gtgctgcga atgcggtac 
      ccccatcga gcccaaccc ctcgaacat gtgcgtgcc 
      ttgcctccg caccaaagt cgacatcac tgaaggcat 
      ccccaaaca ggtcactct gcagttctg tcgcggctg 
      cgagcgcta ctacagccc cccgtccga atggaccca 
      ctgtgtgct cgaatcaaa agaattact ttcattatg 
      tttgaaaaa actgaaagg tttgagtcg ggttaagtt 
      ggtcgacgc tggctttgt ctggaccga accccactc 
      caaacgcat caaagtcaa gctaagggt gcacggaga 
      agtgctcgg tggggctgt gctggagca ggtgtttat 
      cgttgatta cgtggtcag tcaccacat gtgcgatga 
      ctgtcaccg gtcggaagc ccagaactt ctggcgggc 
      tttggtgca agtgagaca aaaggccga gaataaaaa 
      aacgttcta ttatctgga gcagctgat tttgaagca 
      taaggcgca cgataacac cctggggat taaaccgat 
      tcatgatgg gcttgattt tttctacgc cactgaggc 
      ccacgcccg gaaaatggt cgatttcct tgccgccat 
      gctcccgtg caaatacaa tcattcgaa aaaactaat 
      ctcgcatga tattcacag caacttgta caattacaa 
      attcacata ttcggtgga aatcgttcc ggtgtcgaa 
      agacagttt ggtgtgttt gcccaaaaa actgacgca 
      acaattggg cgggatttc ccccatttg tctggtgta 
      cagggttac caactcgct gcatttgat cgacccatc 
      aagggcgca aattgccga aataaatag cactgttta 
      ttggcgctc tccgttcaa tgccatctg taatccgaa 
      acaactggt cgagtacgt ggttatgga cattgagcc 
      gattatgga taaagaccg taagacttt cccgggaca 
      gggggctat ttcaaataa gcatgtctt gtcggatat 
      ttgggtcgt cagagcgtc tgaattggg ggttagcga 
      caatacaat acacacaag gacacactt ggggcacgt 
      gttgaaacc aggcgacta tgttttggg ttataatct 
      tgaagacag taacataaa tgatggaaa ttttgataa 
      attggacaa gaacagtgt ccctgatgt tgttttggt 
      gaagaaata ttacgatag gagtgagag gaagaggag 
      gagagtgtg gaagttgaa acacatggc tcaggaaga 
      gacggcgtt gatcaccga taacaggga ttataacga 
      gtttttgga cgatttgga ggaagatcc cgatttgcg 
      ccaaaacat taatatttt taaggataa tacgaagca 
      aattcccat cgattcggg tgatgatgt tgatgagaa 
      tgttccaag gataacatt ggcggaaat gttggacga 
      tttggttat tgacgatgt tgaaatgtc cgaagtttg 
      attacagga acctgtaat ttctaagtt atgatttga 
      taaaccacg cttgcaatt tttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY