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Gene information
General information
TC031432
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011034
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid76: 71963..76497
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031432
    iBeetle screen iB_01783
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    potentially confused/crosscontaminated with : eggs of..., iB # (comma separated) 01690
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    potentially confused/crosscontaminated with : eggs of..., iB # (comma separated) 01690
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031432 RA
      MRSSLDSDV TPCVAGQRK KRFASRWSL RHLIYSSPI 
      LNRRRSQNS SGSSKSHRN HNGKDTTDS RLGGSSKDI 
      DRGASHHVE IHQATSRTS VFTTSTKSG EVTLSMECP 
      LCFAELTAE EFWELSGCG HRACIPCLQ QYLKVEITE 
      SRVCISCPE CTELIHPNE IRGIIDDVS LFEKYEDFM 
      VRRVLAIEP DARWCPAPD CGFAVIASE CASCPKLKC 
      LRPGCDSYF CYHCKAEWH PNQTCDAAR AQRSPNIRS 
      SSITYSQDS QHRDDIKPC PRCQVLIVK MDDGSCNHM 
      MCAVCGAEF CWLCMKEIS DLHYLSPSG CTFWGKKPW 
      SRKKKILWQ LGTLVGAPV GIALVAGIT VPAMIIGIP 
      VWVGRKLYT RYKAANKHK RNAAIVGGV IASVSNIIL
    CDS sequence
    • >TC031432 RA
      atgcgtagc tctttggat agcgacgtc actccttgt 
      gtggccggc caacggaaa aaacgtttt gcaagccgt 
      tggtcactc cgacacttg atttattct agtccgatt 
      ctgaacaga agacgaagc cagaattcc tcaggttct 
      tcaaaatcg caccggaat cacaacgga aaagacaca 
      actgatagt agattaggt ggttcgtca aaagatatc 
      gatcgtggt gcttcacat catgttgaa attcatcaa 
      gcaacgagt cgaacatca gtattcaca acaagcaca 
      aagagtggt gaagttacc ttatctatg gagtgtccg 
      ttatgtttt gccgaatta acggccgaa gaattttgg 
      gaattgtct ggatgtggg catcgtgct tgcataccc 
      tgccttcag cagtatctc aaagttgaa ataaccgaa 
      tctcgagtg tgcatatca tgcccggaa tgtaccgaa 
      ctcatacat ccaaatgag atacgtggt atcatcgat 
      gatgtttca ttgtttgaa aagtacgaa gattttatg 
      gttaggaga gttttagcg atcgagccg gacgccaga 
      tggtgtccg gcccccgat tgcggtttt gctgtgata 
      gctagtgaa tgtgcaagt tgtccgaaa ctgaagtgt 
      ttgagacct ggctgcgac tcgtatttt tgttatcat 
      tgtaaggcc gaatggcac cccaatcag acctgtgat 
      gcggcgcgt gcgcagaga tcacccaac attcgctcg 
      tcttctatt acatatagt caagattca caacatcga 
      gacgacatt aaaccctgt ccccggtgt caagttttg 
      atcgtcaaa atggacgat ggttcgtgc aatcatatg 
      atgtgcgcc gtttgcggt gcagaattc tgttggctg 
      tgtatgaaa gaaattagc gatctgcat tatctcagt 
      ccctctgga tgtacgttc tggggcaag aaaccatgg 
      tcccggaaa aagaagatt ttgtggcag ttgggtact 
      cttgtgggt gctcccgtt ggtatagcg ttggttgct 
      ggtataacc gttcctgct atgattata ggtattccg 
      gtttgggtt ggcaggaaa ttgtatacc agatacaaa 
      gctgccaat aaacataaa cggaatgct gccattgtc 
      ggaggtgtc attgcttcg gtaagtaat ataatacta 
      tag
    mRNA sequence
    • >TC031432 RA
      attgaggat ttttttgct tgcaggagt catcgaaaa 
      tgtcgttag gataagtag agtttaagt gaaaaaaaa 
      aaaaatcag gatgcgtag ctctttgga tagcgacgt 
      cactccttg tgtggccgg ccaacggaa aaaacgttt 
      tgcaagccg ttggtcact ccgacactt gatttattc 
      tagtccgat tctgaacag aagacgaag ccagaattc 
      ctcaggttc ttcaaaatc gcaccggaa tcacaacgg 
      aaaagacac aactgatag tagattagg tggttcgtc 
      aaaagatat cgatcgtgg tgcttcaca tcatgttga 
      aattcatca agcaacgag tcgaacatc agtattcac 
      aacaagcac aaagagtgg tgaagttac cttatctat 
      ggagtgtcc gttatgttt tgccgaatt aacggccga 
      agaattttg ggaattgtc tggatgtgg gcatcgtgc 
      ttgcatacc ctgccttca gcagtatct caaagttga 
      aataaccga atctcgagt gtgcatatc atgcccgga 
      atgtaccga actcataca tccaaatga gatacgtgg 
      tatcatcga tgatgtttc attgtttga aaagtacga 
      agattttat ggttaggag agttttagc gatcgagcc 
      ggacgccag atggtgtcc ggcccccga ttgcggttt 
      tgctgtgat agctagtga atgtgcaag ttgtccgaa 
      actgaagtg tttgagacc tggctgcga ctcgtattt 
      ttgttatca ttgtaaggc cgaatggca ccccaatca 
      gacctgtga tgcggcgcg tgcgcagag atcacccaa 
      cattcgctc gtcttctat tacatatag tcaagattc 
      acaacatcg agacgacat taaaccctg tccccggtg 
      tcaagtttt gatcgtcaa aatggacga tggttcgtg 
      caatcatat gatgtgcgc cgtttgcgg tgcagaatt 
      ctgttggct gtgtatgaa agaaattag cgatctgca 
      ttatctcag tccctctgg atgtacgtt ctggggcaa 
      gaaaccatg gtcccggaa aaagaagat tttgtggca 
      gttgggtac tcttgtggg tgctcccgt tggtatagc 
      gttggttgc tggtataac cgttcctgc tatgattat 
      aggtattcc ggtttgggt tggcaggaa attgtatac 
      cagatacaa agctgccaa taaacataa acggaatgc 
      tgccattgt cggaggtgt cattgcttc ggtaagtaa 
      tataatact atagagttt tatcactcg ctacgttcg 
      tatcgtgtt gtaaaattt actttcaaa gtctctaca 
      ataaaactt ttaattaga t