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Gene information
General information
TC031441
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011019
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid76: 372840..387667
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031441
    iBeetle screen iB_01777
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    wings bent - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031441 RA
      MTTNIQQGS LLDVLKKKM RQTKEEMER YKDECEEYN 
      KRLHAECMR REEAESEVA ALNRRIQLL EEDLERSEE 
      RLATATAKL AEASAAADE SERQRKVLE NRSLADEER 
      MDALENQLK EARFLAEEA DKKYDEVAR KLVLMEQDL 
      ERAEERAEQ SESKIVELE EELRVVGNN LKSLEVSEE 
      KANQREEEY KNQIKNLTT RLKEATLKE EEYSVTLKQ 
      VEQRLAEAA VTREHSEDK IRAMSDKLR EAEARAEFA 
      ERSVQKLQK EVDRLEDEL VAEKERYKE IGDDLDTAF 
      VELIL
    • >TC031441 RB
      MTTNIQQGS LLDVLKKKM RQTKEEMER YKDECEEYN 
      KRLHAECMR REEAESEVA ALNRRIQLL EEDLERSEE 
      RLATATAKL AEASAAADE SERIRKALE NRTNMEDDR 
      VGILETQLS QAKLIAEEA DKKYEEIFS KVRALGTVR 
      QRKVLENRS LADEERMDA LENQLKEAR FLAEEADKK 
      YDEVARKLV LMEQDLERA EERAEQSES KIVELEEEL 
      RVVGNNLKS LEVSEEKAN QREEEYKNQ IKNLTTRLK 
      EATLKEEEY SVTLKQVEQ RLAEAAVTR EHSEDKIRA 
      MSDKLREAE ARAEFAERS VQKLQKEVD RLEDELVAE 
      KERYKEIGD DLDTAFVEL IL
    CDS sequence
    • >TC031441 RA
      atgacgacg aatatacag caggggtct ttattagac 
      gtgcttaag aaaaaaatg cggcaaacg aaggaggaa 
      atggagcgc tacaaggac gagtgcgag gaatataac 
      aaacgactt catgcggaa tgtatgcga cgagaggaa 
      gctgaatca gaagtagct gccctcaac cgtcgcatc 
      cagttgttg gaggaagat ttggagcgt tcggaagag 
      cgtctggcc accgccacc gccaaactg gccgaagct 
      tccgccgcc gccgatgag agcgaaagg caacgcaag 
      gttctcgag aaccgctcc ctcgccgat gaagagcgc 
      atggacgcc ctcgagaat cagctgaag gaggcccga 
      ttcttggct gaagaagcc gataaaaaa tacgatgag 
      gttgcccgc aaattggtt ctaatggaa caagattta 
      gaacgtgcc gaagaacgg gccgaacaa agcgaaagc 
      aaaatcgta gagcttgag gaggaactt cgcgtcgtt 
      ggtaacaac ctgaaatcc cttgaagtg tctgaagaa 
      aaggccaac cagcgtgaa gaagaatac aaaaatcaa 
      attaagaac ttgaccact cgcctaaag gaggccact 
      ttaaaagag gaagaatat tcggtgacg ctcaaacag 
      gtggaacaa cgattagca gaggcggct gttaccaga 
      gaacatagc gaggataaa atccgtgcc atgtctgat 
      aagctgcgt gaagctgaa gcccgtgct gagtttgct 
      gaacgttcc gtacagaaa ctgcagaag gaggtcgac 
      agactagaa gatgagctc gtcgccgag aaggagcgc 
      tacaaggaa atcggcgac gatttggac acggctttc 
      gtcgaactt atcttgtaa
    • >TC031441 RB
      atgacgacg aatatacag caggggtct ttattagac 
      gtgcttaag aaaaaaatg cggcaaacg aaggaggaa 
      atggagcgc tacaaggac gagtgcgag gaatataac 
      aaacgactt catgcggaa tgtatgcga cgagaggaa 
      gctgaatca gaagtagct gccctcaac cgtcgcatc 
      cagttgttg gaggaagat ttggagcgt tcggaagag 
      cgtctggcc accgccacc gccaaactg gccgaagct 
      tccgccgcc gccgatgag agcgaaagg atacgcaaa 
      gccttagaa aaccgaacc aacatggag gacgatcga 
      gtaggcatc ttggaaacc caactgtct caagctaaa 
      ctcatagcc gaggaagcc gacaaaaaa tacgaagag 
      attttcagc aaagttagg gctttaggg accgttagg 
      caacgcaag gttctcgag aaccgctcc ctcgccgat 
      gaagagcgc atggacgcc ctcgagaat cagctgaag 
      gaggcccga ttcttggct gaagaagcc gataaaaaa 
      tacgatgag gttgcccgc aaattggtt ctaatggaa 
      caagattta gaacgtgcc gaagaacgg gccgaacaa 
      agcgaaagc aaaatcgta gagcttgag gaggaactt 
      cgcgtcgtt ggtaacaac ctgaaatcc cttgaagtg 
      tctgaagaa aaggccaac cagcgtgaa gaagaatac 
      aaaaatcaa attaagaac ttgaccact cgcctaaag 
      gaggccact ttaaaagag gaagaatat tcggtgacg 
      ctcaaacag gtggaacaa cgattagca gaggcggct 
      gttaccaga gaacatagc gaggataaa atccgtgcc 
      atgtctgat aagctgcgt gaagctgaa gcccgtgct 
      gagtttgct gaacgttcc gtacagaaa ctgcagaag 
      gaggtcgac agactagaa gatgagctc gtcgccgag 
      aaggagcgc tacaaggaa atcggcgac gatttggac 
      acggctttc gtcgaactt atcttgtaa
    mRNA sequence
    • >TC031441 RA
      cccagttgt tcgaagtcg gtgtcggtg cgtggtaat 
      cgggtcgga gttagtggt ttttttcgt aattttgtt 
      aaaaaaatc gtaaaaatg acgacgaat atacagcag 
      gggtcttta ttagacgtg cttaagaaa aaaatgcgg 
      caaacgaag gaggaaatg gagcgctac aaggacgag 
      tgcgaggaa tataacaaa cgacttcat gcggaatgt 
      atgcgacga gaggaagct gaatcagaa gtagctgcc 
      ctcaaccgt cgcatccag ttgttggag gaagatttg 
      gagcgttcg gaagagcgt ctggccacc gccaccgcc 
      aaactggcc gaagcttcc gccgccgcc gatgagagc 
      gaaaggcaa cgcaaggtt ctcgagaac cgctccctc 
      gccgatgaa gagcgcatg gacgccctc gagaatcag 
      ctgaaggag gcccgattc ttggctgaa gaagccgat 
      aaaaaatac gatgaggtt gcccgcaaa ttggttcta 
      atggaacaa gatttagaa cgtgccgaa gaacgggcc 
      gaacaaagc gaaagcaaa atcgtagag cttgaggag 
      gaacttcgc gtcgttggt aacaacctg aaatccctt 
      gaagtgtct gaagaaaag gccaaccag cgtgaagaa 
      gaatacaaa aatcaaatt aagaacttg accactcgc 
      ctaaaggag gccacttta aaagaggaa gaatattcg 
      gtgacgctc aaacaggtg gaacaacga ttagcagag 
      gcggctgtt accagagaa catagcgag gataaaatc 
      cgtgccatg tctgataag ctgcgtgaa gctgaagcc 
      cgtgctgag tttgctgaa cgttccgta cagaaactg 
      cagaaggag gtcgacaga ctagaagat gagctcgtc 
      gccgagaag gagcgctac aaggaaatc ggcgacgat 
      ttggacacg gctttcgtc gaacttatc ttgtaaaga 
      atcaatcgt ttctcttcc agcgactct gtcttttta 
      ttttggtaa ttgtgttac gtgttgtat gtacaggaa 
      gcaaaatat attgagtta ctgatcacc ctttttacc 
      ttattttaa cactctaac cgtactaac atttccaga 
      tgaacttgt cgacgagaa gaccaaata caaagcgat 
      cgccgacga aatggacca aactatggc cgatttggc 
      tggatatta agtctgttt ttccgacaa actgccgct 
      gaaactgcc taattcgtt cagatgctc acgcaagtc 
      tggcatctt gcttggtgt tggactgca accgttggc 
      tttcgcgtt ttcaatgca ctctcattt agctaagtt 
      tgggcaagt cttcgtttt gcttgtagc cggttttac 
      agtgttacc actagggcg agtcgacaa gatttaata 
      ctcatattt ttattaaca ataaatgat tttggaatg 
      g
    • >TC031441 RB
      cccagttgt tcgaagtcg gtgtcggtg cgtggtaat 
      cgggtcgga gttagtggt ttttttcgt aattttgtt 
      aaaaaaatc gtaaaaatg acgacgaat atacagcag 
      gggtcttta ttagacgtg cttaagaaa aaaatgcgg 
      caaacgaag gaggaaatg gagcgctac aaggacgag 
      tgcgaggaa tataacaaa cgacttcat gcggaatgt 
      atgcgacga gaggaagct gaatcagaa gtagctgcc 
      ctcaaccgt cgcatccag ttgttggag gaagatttg 
      gagcgttcg gaagagcgt ctggccacc gccaccgcc 
      aaactggcc gaagcttcc gccgccgcc gatgagagc 
      gaaaggata cgcaaagcc ttagaaaac cgaaccaac 
      atggaggac gatcgagta ggcatcttg gaaacccaa 
      ctgtctcaa gctaaactc atagccgag gaagccgac 
      aaaaaatac gaagagatt ttcagcaaa gttagggct 
      ttagggacc gttaggcaa cgcaaggtt ctcgagaac 
      cgctccctc gccgatgaa gagcgcatg gacgccctc 
      gagaatcag ctgaaggag gcccgattc ttggctgaa 
      gaagccgat aaaaaatac gatgaggtt gcccgcaaa 
      ttggttcta atggaacaa gatttagaa cgtgccgaa 
      gaacgggcc gaacaaagc gaaagcaaa atcgtagag 
      cttgaggag gaacttcgc gtcgttggt aacaacctg 
      aaatccctt gaagtgtct gaagaaaag gccaaccag 
      cgtgaagaa gaatacaaa aatcaaatt aagaacttg 
      accactcgc ctaaaggag gccacttta aaagaggaa 
      gaatattcg gtgacgctc aaacaggtg gaacaacga 
      ttagcagag gcggctgtt accagagaa catagcgag 
      gataaaatc cgtgccatg tctgataag ctgcgtgaa 
      gctgaagcc cgtgctgag tttgctgaa cgttccgta 
      cagaaactg cagaaggag gtcgacaga ctagaagat 
      gagctcgtc gccgagaag gagcgctac aaggaaatc 
      ggcgacgat ttggacacg gctttcgtc gaacttatc 
      ttgtaaaga atcaatcgt ttctcttcc agcgactct 
      gtcttttta ttttggtaa ttgtgttac gtgttgtat 
      gtacaggaa gcaaaatat attgagtta ctgatcacc 
      ctttttacc ttattttaa cactctaac cgtactaac 
      atttccaga tgaacttgt cgacgagaa gaccaaata 
      caaagcgat cgccgacga aatggacca aactatggc 
      cgatttggc tggatatta agtctgttt ttccgacaa 
      actgccgct gaaactgcc taattcgtt cagatgctc 
      acgcaagtc tggcatctt gcttggtgt tggactgca 
      accgttggc tttcgcgtt ttcaatgca ctctcattt 
      agctaagtt tgggcaagt cttcgtttt gcttgtagc 
      cggttttac agtgttacc actagggcg agtcgacaa 
      gatttaata ctcatattt ttattaaca ataaatgat 
      tttggaatg g