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Gene information
General information
TC011012 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid76: 565186..567117
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011012
    iBeetle screen iB_01776
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Fertility
    vitellarium shortened - Number of animals showing the phenotype: 3

    vitellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4

    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 3

    previtellogenic egg chamber sometimes deposition irregular - Number of animals showing the phenotype: 2

    Comment: Early oocytes are not lined up, but appear accumulated right below the tropharium.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    Pupal morphology
    wings bent - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011012 RA
      MDQLGDTPR LNYLDPRLS NSNVPLPVK IDKEKDQQI 
      IDKWLVERG KFQINPYYS IRRPRCEIT LSGAKQALK 
      NCLAIVEKL NKTQEELRN NVETMSSNE WKQKTLDIG 
      SLKDEFDKS ILLFEDNTI LKSLKGLAV KRARKRLNR 
      KKQKKFRQE QLKVKKEKQ SEIDKSINT WLESKKDEV 
      ERAKMEEKM KRDADCVLY EVTKKKSEA RKQLSLISA 
      LVKLRTVRD AMATQRGDK LSLEDRQAF GHVTEKLIR 
      MWENSYQSY CKEEQCLKV MLEHNASKD SKAAIAAKQ 
      TRIVDDWES LLFGPRDPP VNNPTFWAL TSADRDLET 
      FIAIRKSWD TFLSPGNAS GSAIPLGWV APPEAPSES 
      WAPFLKTD
    CDS sequence
    • >TC011012 RA
      atggatcag ctcggggac acccccaga cttaactat 
      ctcgacccc cgactttcc aactccaac gtgccactt 
      cccgtcaaa atcgacaag gaaaaagac caacaaatc 
      atcgataaa tggctcgtt gaaagaggc aagttccag 
      ataaacccc tactattcc atacgcagg ccacggtgc 
      gaaatcacg ctaagcggg gcgaagcaa gccttgaaa 
      aactgtctc gcaatcgtg gaaaaactc aacaagacg 
      caggaagag ctccggaat aacgttgaa accatgtcg 
      tccaacgaa tggaagcag aaaacgctc gacatcggc 
      agtctcaag gacgagttc gataaatca atcctttta 
      tttgaagac aacacgatt ctaaagtca ctcaaaggc 
      ctggccgtc aagcgggcg cgtaagcgc ctcaatcgc 
      aagaaacag aaaaagttc cggcaagaa caactcaaa 
      gtcaagaag gagaaacaa agcgagatt gataaaagc 
      ataaacacg tggcttgag agcaaaaag gacgaagtg 
      gaacgcgcg aaaatggaa gaaaagatg aagagagac 
      gccgattgt gttttgtac gaagtgacg aagaagaaa 
      tcggaagcg cgcaagcaa ctctcactc atttccgcg 
      ctcgttaag ttgcggacg gttagagac gccatggcc 
      acgcaaagg ggcgacaag ttgtcgctg gaggacagg 
      caggccttc gggcacgtc acggagaaa ctaattcgc 
      atgtgggag aactcgtac cagagctac tgcaaagag 
      gagcaatgc ttgaaagtg atgctggaa cacaacgcc 
      agtaaggac tccaaagcg gcgatcgcc gccaaacaa 
      acccgcatt gtcgacgac tgggagtcc ctcctattc 
      ggcccccga gaccccccc gtcaacaac cccaccttc 
      tgggctcta acgagcgcc gaccgcgac ttggagaca 
      tttatcgcc ataaggaaa agttgggac acgtttcta 
      agtccgggg aacgcctca ggctcggct attccactc 
      gggtgggtc gccccgccc gaggccccc agcgagagt 
      tgggccccg tttttaaaa accgactaa
    mRNA sequence
    • >TC011012 RA
      tgggccacc gggccccag tgtcagcgg ccatttttt 
      gttgttcat gtccacatc cacccgcaa acactcaat 
      tggtgcaaa aaaccaaat tttgtgact cacaattcg 
      gaagagtga atcatttaa aacagtaat ttctattgt 
      gtttctcta tgaccagtg acaaagatc acaaaaccg 
      tgtttttga acgcataaa gtcacactt ctgtccgtc 
      atttgggtg ttatggccg atcctcgca taaaaactc 
      tcaaaatca aaacaggtg tagtgaaga ggttagcca 
      aagaaaaag taacgtatg agaaagaag ccgatcagc 
      aaagaaaca gaatcgaga aattgaaac gtgagggta 
      aggacgaat atgacatca gaaagcagg aggaggtcc 
      tgcaggaga gtctgatga tggtccctg actgccaga 
      gacgccttg cacttgctt tcgacgaac tgaacaaaa 
      tcctcgcct ctgagcagg atttgaagg aacttgagg 
      agtaccaaa ctgctttga acgtgctag aggaggcaa 
      agttgcaat tgccacaaa aagattaat tcgttgttt 
      tcgtaaatt agttaaaaa taatggatc agctcgggg 
      acaccccca gacttaact atctcgacc cccgacttt 
      ccaactcca acgtgccac ttcccgtca aaatcgaca 
      aggaaaaag accaacaaa tcatcgata aatggctcg 
      ttgaaagag gcaagttcc agataaacc cctactatt 
      ccatacgca ggccacggt gcgaaatca cgctaagcg 
      gggcgaagc aagccttga aaaactgtc tcgcaatcg 
      tggaaaaac tcaacaaga cgcaggaag agctccgga 
      ataacgttg aaaccatgt cgtccaacg aatggaagc 
      agaaaacgc tcgacatcg gcagtctca aggacgagt 
      tcgataaat caatccttt tatttgaag acaacacga 
      ttctaaagt cactcaaag gcctggccg tcaagcggg 
      cgcgtaagc gcctcaatc gcaagaaac agaaaaagt 
      tccggcaag aacaactca aagtcaaga aggagaaac 
      aaagcgaga ttgataaaa gcataaaca cgtggcttg 
      agagcaaaa aggacgaag tggaacgcg cgaaaatgg 
      aagaaaaga tgaagagag acgccgatt gtgttttgt 
      acgaagtga cgaagaaga aatcggaag cgcgcaagc 
      aactctcac tcatttccg cgctcgtta agttgcgga 
      cggttagag acgccatgg ccacgcaaa ggggcgaca 
      agttgtcgc tggaggaca ggcaggcct tcgggcacg 
      tcacggaga aactaattc gcatgtggg agaactcgt 
      accagagct actgcaaag aggagcaat gcttgaaag 
      tgatgctgg aacacaacg ccagtaagg actccaaag 
      cggcgatcg ccgccaaac aaacccgca ttgtcgacg 
      actgggagt ccctcctat tcggccccc gagaccccc 
      ccgtcaaca accccacct tctgggctc taacgagcg 
      ccgaccgcg acttggaga catttatcg ccataagga 
      aaagttggg acacgtttc taagtccgg ggaacgcct 
      caggctcgg ctattccac tcgggtggg tcgccccgc 
      ccgaggccc ccagcgaga gttgggccc cgtttttaa 
      aaaccgact aatgctttt ctattattt ttaataaaa 
      taatcacaa ttaa