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Gene information
General information
TC011003 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid76: 707153..711559
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011003
    iBeetle screen iB_01775
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Analysis of embryonic musculature and early development
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011003 RA
      MKFLVFVAL CVVGSQAVS FFDLVQEQW GAFKVTHKK 
      QYESETEER FRMKIFMEN AHKVAKHNK LYAQGLVSF 
      KLGVNKYSD MLNHEFVHT LNGYNRSKT PLRSGELDE 
      SITFIPPAN VELPKQIDW RKLGAVTPV KDQGQCGSC 
      WSFSTTGSL EGQHFRKSK KLVSLSEQN LIDCSEKYG 
      NNGCNGGLM DNAFRYIKD NGGIDTEQS YPYKAEDEK 
      CHYKPRNKG ATDRGFVDI ESGDEEKLK AAVATVGPI 
      SVAIDASHP TFQQYSEGV YYEPECSSE QLDHGVLVV 
      GYGTDEDGN DYWLVKNSW GDSWGDQGY IKMARNRDN 
      NCGIATQAS YPLV
    CDS sequence
    • >TC011003 RA
      atgaagttc ctggtgttt gtggccctt tgtgtggtc 
      ggcagtcaa gccgtgtct ttttttgac ctagtccag 
      gaacaatgg ggcgctttc aaggtgact cacaagaaa 
      caatacgaa agcgaaacc gaagaacgc ttccgtatg 
      aaaatcttc atggaaaat gcccacaaa gtcgccaaa 
      cataacaaa ttgtacgct cagggttta gtttcgttt 
      aaattaggc gtaaataaa tactccgat atgcttaat 
      catgaattt gttcacaca ttaaatggt tacaatcgc 
      tctaaaacc ccactgaga agtggcgaa ttggacgaa 
      tctatcact ttcattcca cctgcaaac gtcgaatta 
      ccgaaacaa atcgactgg agaaaactt ggggccgtc 
      acacccgtc aaggaccaa ggacaatgc ggctcatgc 
      tggagcttc agtacgact ggttccctc gagggccag 
      cacttccgc aagtccaaa aaactcgtc tccctcagc 
      gaacaaaac ctgatcgac tgttcggaa aagtacgga 
      aataacggc tgtaacggc ggtctcatg gacaacgct 
      ttccgttac attaaagac aacggcgga attgacacc 
      gaacagtcg tatccgtac aaagccgaa gacgagaaa 
      tgccactac aagcccaga aataagggc gccactgac 
      cgcggattc gtcgatatc gagtcaggt gatgaggaa 
      aagctcaaa gctgcagtt gcgacagtt gggccaatt 
      tctgttgca attgatgca agtcatccc actttccag 
      cagtattcc gaaggggtg tactatgag cctgagtgt 
      agctcggag cagctggat catggggtt ctggtggtg 
      gggtatggg accgatgag gatggtaat gattattgg 
      ttggtgaaa aattcatgg ggggacagt tggggcgat 
      caggggtac atcaagatg gctcgcaat cgggataac 
      aattgcgga attgctacc caagctagt tatccgtta 
      gtttag
    mRNA sequence
    • >TC011003 RA
      tggtcgtga tggtggggg acttcctgg tcagctgac 
      ttttatgta caaaacaaa tttgactca ttaaaattt 
      tctgctcat ttcgagtgc tggtgtgag ttggagagg 
      agcagtcga acaaattat aactaagaa ccatgaagt 
      tcctggtgt ttgtggccc tttgtgtgg tcggcagtc 
      aagccgtgt ctttttttg acctagtcc aggaacaat 
      ggggcgctt tcaaggtga ctcacaaga aacaatacg 
      aaagcgaaa ccgaagaac gcttccgta tgaaaatct 
      tcatggaaa atgcccaca aagtcgcca aacataaca 
      aattgtacg ctcagggtt tagtttcgt ttaaattag 
      gcgtaaata aatactccg atatgctta atcatgaat 
      ttgttcaca cattaaatg gttacaatc gctctaaaa 
      ccccactga gaagtggcg aattggacg aatctatca 
      ctttcattc cacctgcaa acgtcgaat taccgaaac 
      aaatcgact ggagaaaac ttggggccg tcacacccg 
      tcaaggacc aaggacaat gcggctcat gctggagct 
      tcagtacga ctggttccc tcgagggcc agcacttcc 
      gcaagtcca aaaaactcg tctccctca gcgaacaaa 
      acctgatcg actgttcgg aaaagtacg gaaataacg 
      gctgtaacg gcggtctca tggacaacg ctttccgtt 
      acattaaag acaacggcg gaattgaca ccgaacagt 
      cgtatccgt acaaagccg aagacgaga aatgccact 
      acaagccca gaaataagg gcgccactg accgcggat 
      tcgtcgata tcgagtcag gtgatgagg aaaagctca 
      aagctgcag ttgcgacag ttgggccaa tttctgttg 
      caattgatg caagtcatc ccactttcc agcagtatt 
      ccgaagggg tgtactatg agcctgagt gtagctcgg 
      agcagctgg atcatgggg ttctggtgg tggggtatg 
      ggaccgatg aggatggta atgattatt ggttggtga 
      aaaattcat ggggggaca gttggggcg atcaggggt 
      acatcaaga tggctcgca atcgggata acaattgcg 
      gaattgcta cccaagcta gttatccgt tagtttagg 
      tttatttct gtgttttaa gttcgaata gttattaga 
      tgtttttga tttgtttgt gatttttgt atttttttt 
      aattgtaat ctcgttttt gaacaaata aactcgaac 
      aattttt