Status
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Gene information
General information
TC010997 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450827.1: 3645..47189
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010997
    iBeetle screen iB_01773

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    iBeetle screen iB_04961

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010997 RA
      MDAKFHRVK TKDTDEEEE FAPSEVYRI WKNVLVIGF 
      AFMIHFTAF WGTSNLQSS INADDALGT FTLAAIYAS 
      LILSNVFLP TIVIRWFGC KWTIALSFI TYMPFIIAQ 
      FYPKFYTMI PAGLAVGFG GGPLWCAKC TYLTVISEA 
      YGQLTGTSP EIAVTRFFG VFFMFYQFS QVWGNLISS 
      AVLSSGGIS EPTALLTNT TVSNLTSLL SAETVVTSL 
      QDERPNDLC GANFCPGTV TEVANPNLH PPETSKIHL 
      ITGIYLACM VGACLVVAI FVDPMNRYG KGRKGSGSG 
      LSGIKLLAV TLKQLAHPY QILILPITM FIGAEQAFI 
      AADFNSLKL RDGYERNIV SNSMQQLHL TKSIPYKNN 
      SLMTTLDNS LDSILVQIG DFGKYQSFM FSLVCFGVI 
      LHSGVHVAF VFTAMDVSY RCEIPQCDT VDPQYEPPW 
      LPNAVPYTD TNPAKCTRY IINATQDFG NCTKEGDFT 
      SLTERCDTF VYETREKSI LQEYNLQCD DNLWKLTLV 
      GTINNVGQF FGLFISGII SDRFGRKVI FIWGLILCG 
      VCGLLRTLA PTYIWFLVF EFLDAAFGA GSYVCGFIM 
      GVELVGPKK RVLTGTLVS SCYALGEVF AAGSAWFFE 
      DWRPIIYVL YGPPMLLFV YFWIIPESI RWNISKGKI 
      EEAKDTLRR VAKVNGKTI SEDALDKLA MADTGDSNK 
      SSISEVFKS PVLMLRLIN CFFCWITCA FLFYGLTLN 
      SVALAGNGY LDFILTSLV EIPAYFACI YVVEKMGRK 
      WSLSLSFFM TGISCCVFI FIPKDLHNV SLAMWMLGK 
      FGATAAFTI TYVITSELF PTPLRHSLM GACSTFGRI 
      GSMIAPQTP LLAQIWEPL PIIMYTAMA TIAGLLTLL 
      FPETVNIKL PDTIDEAIS IGKNKNSVP KD
    CDS sequence
    • >TC010997 RA
      atggatgca aaattccac cgtgttaaa accaaagat 
      accgatgaa gaggaagaa tttgcaccg agtgaagtt 
      taccgaatt tggaaaaat gttcttgtg attggattt 
      gctttcatg atacatttc accgcattt tggggcact 
      tcgaatttg caaagctcc attaatgcg gacgatgct 
      ctgggaacg tttactttg gctgcaatc tacgcatct 
      ctaatatta tccaacgtg ttccttcca actatcgta 
      atcagatgg tttggatgc aaatggacc atcgcttta 
      tccttcatc acttacatg cccttcatc atagcccag 
      ttttaccca aaattctac acaatgatc ccagctggt 
      ttagcggtt ggttttgga ggaggtcct ttatggtgt 
      gcaaagtgt acatatttg acggttatt tccgaagct 
      tatggacaa cttaccgga acttcgccc gaaattgca 
      gtcacacgt ttcttcggt gtatttttc atgttctat 
      cagttctca caagtgtgg ggaaacctc atcagttca 
      gcagttctc tcctctgga ggtatctct gaaccgacc 
      gcacttctg accaacaca acggtctca aacctaacg 
      tcattgtta tccgccgaa accgtggtt acatccttg 
      caggatgaa agaccaaat gatttatgt ggcgccaat 
      ttctgtcca ggaacggta accgaagtg gcaaaccct 
      aatctgcac cctcctgaa acatcaaaa attcatcta 
      atcactgga atttatttg gcttgtatg gttggagca 
      tgtctagtt gtggcgatt tttgttgat ccgatgaat 
      agatatgga aaagggcgc aaaggcagt gggagtggg 
      ctctctggt attaaattg ttggcagtt actttaaaa 
      caacttgct catccttat caaatttta atacttcca 
      attacaatg tttattggg gctgaacaa gctttcatt 
      gctgccgac ttcaattcg ttgaagttg cgggacggt 
      tacgaacgc aacattgtt tcaaattca atgcaacaa 
      ttacattta actaaaagt attccatat aagaacaac 
      agtctgatg accacacta gacaactcc ttggactcg 
      attttggtc caaattggc gattttgga aaatatcaa 
      tcgtttatg tttagttta gtttgtttt ggtgttatt 
      ttacactct ggagttcat gtggcgttc gtttttact 
      gccatggat gttagttat cggtgcgaa attccccaa 
      tgtgacaca gttgaccca caatatgaa ccaccttgg 
      ttaccaaac gctgtacct tataccgat acgaatcca 
      gctaaatgc acaagatac ataatcaat gccacgcaa 
      gattttggc aattgcaca aaagaaggc gatttcaca 
      agtttgaca gaaagatgc gatacattt gtttacgaa 
      acacgagaa aaaagcatt ttacaggag tataatctc 
      caatgcgat gataatctg tggaaactg acgttagtt 
      ggtaccatc aataacgtg ggacagttt ttcggattg 
      tttatttca ggaatcata tcagacagg tttggccgt 
      aaagttata tttatttgg ggccttata ctatgcgga 
      gtttgtgga ttacttcga acgcttgct cctacttac 
      atttggttt ttagtattt gaatttttg gatgctgcg 
      tttggtgcc ggcagttac gtttgcgga tttattatg 
      ggtgttgaa ctagttgga ccaaagaaa agagtacta 
      actggtacg ttagtaagt tcctgttat gctttgggg 
      gaagttttt gccgctggt tcagcatgg ttttttgaa 
      gactggaga ccgattatt tacgtactg tacggtcct 
      ccaatgttg ttgtttgtc tatttttgg ataatccca 
      gaatcaatt agatggaat atcagtaaa ggaaaaata 
      gaagaagcc aaagacact ctgagacgt gtcgcaaaa 
      gtcaatgga aaaactata tctgaagac gctttagat 
      aaactggca atggctgac acaggcgat tctaacaaa 
      tcttcaatc tctgaagtg ttcaaatct ccggttttg 
      atgcttcgt ctaatcaat tgcttcttc tgttggatc 
      acttgtgca tttcttttt tacggctta acgctgaat 
      tctgtcgcc ttggcaggc aatggctat ttagatttt 
      attttaact tcgttagtt gaaattccg gcttatttt 
      gcatgtatt tatgttgtt gaaaaaatg ggacgaaag 
      tggtccctg agtttgtca tttttcatg actggtatc 
      tcctgttgt gtgtttatt ttcattccg aaagattta 
      cacaatgtt agccttgca atgtggatg cttggaaaa 
      tttggcgct acagctgcg tttacaata acctacgtt 
      ataacaagt gaattgttt ccaacgcca ttgcgacat 
      tctttaatg ggggcatgc tcaactttt ggaaggata 
      ggctctatg atagcaccg caaacccca ttgctcgct 
      caaatttgg gaacctctg ccaattata atgtacaca 
      gcaatggcc actatcgca ggacttttg acgcttcta 
      tttccagag actgtaaac attaagcta ccagatacg 
      atagatgaa gccattagt ataggaaaa aataaaaat 
      agtgtacct aaagactaa
    mRNA sequence
    • >TC010997 RA
      tccagtcgg cacaagtgg cggtttatc aaatagttt 
      agtgttagt tagtgccaa tttggtctc ttatttcga 
      ttttcctaa gaagtcaag aggtcatgg atgcaaaat 
      tccaccgtg ttaaaacca aagataccg atgaagagg 
      aagaatttg caccgagtg aagtttacc gaatttgga 
      aaaatgttc ttgtgattg gatttgctt tcatgatac 
      atttcaccg cattttggg gcacttcga atttgcaaa 
      gctccatta atgcggacg atgctctgg gaacgttta 
      ctttggctg caatctacg catctctaa tattatcca 
      acgtgttcc ttccaacta tcgtaatca gatggtttg 
      gatgcaaat ggaccatcg ctttatcct tcatcactt 
      acatgccct tcatcatag cccagtttt acccaaaat 
      tctacacaa tgatcccag ctggtttag cggttggtt 
      ttggaggag gtcctttat ggtgtgcaa agtgtacat 
      atttgacgg ttatttccg aagcttatg gacaactta 
      ccggaactt cgcccgaaa ttgcagtca cacgtttct 
      tcggtgtat ttttcatgt tctatcagt tctcacaag 
      tgtggggaa acctcatca gttcagcag ttctctcct 
      ctggaggta tctctgaac cgaccgcac ttctgacca 
      acacaacgg tctcaaacc taacgtcat tgttatccg 
      ccgaaaccg tggttacat ccttgcagg atgaaagac 
      caaatgatt tatgtggcg ccaatttct gtccaggaa 
      cggtaaccg aagtggcaa accctaatc tgcaccctc 
      ctgaaacat caaaaattc atctaatca ctggaattt 
      atttggctt gtatggttg gagcatgtc tagttgtgg 
      cgatttttg ttgatccga tgaatagat atggaaaag 
      ggcgcaaag gcagtggga gtgggctct ctggtatta 
      aattgttgg cagttactt taaaacaac ttgctcatc 
      cttatcaaa ttttaatac ttccaatta caatgttta 
      ttggggctg aacaagctt tcattgctg ccgacttca 
      attcgttga agttgcggg acggttacg aacgcaaca 
      ttgtttcaa attcaatgc aacaattac atttaacta 
      aaagtattc catataaga acaacagtc tgatgacca 
      cactagaca actccttgg actcgattt tggtccaaa 
      ttggcgatt ttggaaaat atcaatcgt ttatgttta 
      gtttagttt gttttggtg ttattttac actctggag 
      ttcatgtgg cgttcgttt ttactgcca tggatgtta 
      gttatcggt gcgaaattc cccaatgtg acacagttg 
      acccacaat atgaaccac cttggttac caaacgctg 
      taccttata ccgatacga atccagcta aatgcacaa 
      gatacataa tcaatgcca cgcaagatt ttggcaatt 
      gcacaaaag aaggcgatt tcacaagtt tgacagaaa 
      gatgcgata catttgttt acgaaacac gagaaaaaa 
      gcattttac aggagtata atctccaat gcgatgata 
      atctgtgga aactgacgt tagttggta ccatcaata 
      acgtgggac agtttttcg gattgttta tttcaggaa 
      tcatatcag acaggtttg gccgtaaag ttatattta 
      tttggggcc ttatactat gcggagttt gtggattac 
      ttcgaacgc ttgctccta cttacattt ggtttttag 
      tatttgaat ttttggatg ctgcgtttg gtgccggca 
      gttacgttt gcggattta ttatgggtg ttgaactag 
      ttggaccaa agaaaagag tactaactg gtacgttag 
      taagttcct gttatgctt tgggggaag tttttgccg 
      ctggttcag catggtttt ttgaagact ggagaccga 
      ttatttacg tactgtacg gtcctccaa tgttgttgt 
      ttgtctatt tttggataa tcccagaat caattagat 
      ggaatatca gtaaaggaa aaatagaag aagccaaag 
      acactctga gacgtgtcg caaaagtca atggaaaaa 
      ctatatctg aagacgctt tagataaac tggcaatgg 
      ctgacacag gcgattcta acaaatctt caatctctg 
      aagtgttca aatctccgg ttttgatgc ttcgtctaa 
      tcaattgct tcttctgtt ggatcactt gtgcatttc 
      ttttttacg gcttaacgc tgaattctg tcgccttgg 
      caggcaatg gctatttag attttattt taacttcgt 
      tagttgaaa ttccggctt attttgcat gtatttatg 
      ttgttgaaa aaatgggac gaaagtggt ccctgagtt 
      tgtcatttt tcatgactg gtatctcct gttgtgtgt 
      ttattttca ttccgaaag atttacaca atgttagcc 
      ttgcaatgt ggatgcttg gaaaatttg gcgctacag 
      ctgcgttta caataacct acgttataa caagtgaat 
      tgtttccaa cgccattgc gacattctt taatggggg 
      catgctcaa cttttggaa ggataggct ctatgatag 
      caccgcaaa ccccattgc tcgctcaaa tttgggaac 
      ctctgccaa ttataatgt acacagcaa tggccacta 
      tcgcaggac ttttgacgc ttctatttc cagagactg 
      taaacatta agctaccag atacgatag atgaagcca 
      ttagtatag gaaaaaata aaaatagtg tacctaaag 
      actaacata tctgtgatg agttttgta aaaaagtac 
      taaacattt taggcgtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY