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Gene information
General information
TC010977 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 2999106..3016913
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010977
    iBeetle screen iB_01770

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature dorsal partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: effect clearly stronger in the abdominal musculature
    abdomen musculature ventral partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    leg musculature sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva cuticle duplicated - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010977 RA
      MAFLLDLMR NVNSYLNKY QDPRTADWP LMSSPFYTL 
      GICLSYVVV VKVLGPKLM ENRKPFQFK KLLVFYNFL 
      QVVFSIWLF YEIGMGGWF TGEYSIRCQ PVDYSNKPS 
      TIRMVHASW WYYFSKFTE FLDTIFFVL RKKYDQVST 
      LHVIHHGVM PMSVWFGVK FTPGGHSTF FGFLNTFVH 
      IIMYFYYML SALGPEVQK YLWWKKYLT TLQMVQFVA 
      IMVHAFQLL FTDCNYPRA FVWWIGMHA VMFFFLFKE 
      FYIQTYNKR TAAAAAAAA AAAAKNGVV QNGTGAHQN 
      GVKSNKVSQ YYVNGTADL HQRIKSQ
    CDS sequence
    • >TC010977 RA
      atggctttt cttctggat ttgatgcgc aatgtaaac 
      tcctattta aacaaatat caagatcca cgcacagca 
      gactggcca ttaatgtca agtcctttt tatacctta 
      ggaatatgt ttatcttat gtagttgta gtaaaggtt 
      ttgggaccg aaactaatg gaaaataga aaacccttc 
      cagtttaag aaacttcta gttttttat aatttttta 
      caagtagta tttagtata tggttattt tatgaaatt 
      ggtatggga ggttggttc acaggtgaa tatagcata 
      aggtgtcaa cctgttgat tattcaaat aaacctagt 
      acaataagg atggtgcac gcgtcatgg tggtactat 
      ttctcgaag tttacagaa tttcttgat actattttt 
      ttcgtattg agaaagaaa tatgatcaa gtcagcacg 
      ttacacgtg atccaccac ggcgtaatg cctatgtcc 
      gtctggttc ggagtcaaa tttacccca ggcggtcat 
      agcaccttt ttcggcttc cttaacaca tttgtgcat 
      atcatcatg tacttctat tacatgcta agtgccctt 
      ggtcccgaa gtccagaag tacttatgg tggaagaag 
      tatttaaca acgttgcaa atggttcaa tttgtggcg 
      atcatggtc catgccttc cagttactg ttcaccgat 
      tgcaactac cctcgtgcc ttcgtctgg tggattggg 
      atgcacgct gtcatgttc ttctttctg tttaaggag 
      ttctacatc caaacttat aataaaaga acagcagca 
      gcggcggcg gcggcggcg gcggcggca gcgaagaac 
      ggcgtcgtc cagaacggc acaggtgcg catcagaac 
      ggagttaaa tcgaacaaa gttagccaa tattatgtc 
      aacggaacg gcggacttg catcaaagg ataaagtcg 
      cagtga
    mRNA sequence
    • >TC010977 RA
      agtttgtgg tgtgtacat agtgagtgt tgttgaaac 
      caccacagc cgtttttag ttttcgtgt gtgttttta 
      gtattagtt ttttctcaa tttctgaat gtatccttt 
      tgcgttctg gtgattttc cggctgtga tcgtcacaa 
      tttaagtcc tgcgttcgc cccttttcc taatttatt 
      cgttaaaat ggcttttct tctggattt gatgcgcaa 
      tgtaaactc ctatttaaa caaatatca agatccacg 
      cacagcaga ctggccatt aatgtcaag tccttttta 
      taccttagg aatatgttt atcttatgt agttgtagt 
      aaaggtttt gggaccgaa actaatgga aaatagaaa 
      acccttcca gtttaagaa acttctagt tttttataa 
      ttttttaca agtagtatt tagtatatg gttatttta 
      tgaaattgg tatgggagg ttggttcac aggtgaata 
      tagcataag gtgtcaacc tgttgatta ttcaaataa 
      acctagtac aataaggat ggtgcacgc gtcatggtg 
      gtactattt ctcgaagtt tacagaatt tcttgatac 
      tattttttt cgtattgag aaagaaata tgatcaagt 
      cagcacgtt acacgtgat ccaccacgg cgtaatgcc 
      tatgtccgt ctggttcgg agtcaaatt taccccagg 
      cggtcatag caccttttt cggcttcct taacacatt 
      tgtgcatat catcatgta cttctatta catgctaag 
      tgcccttgg tcccgaagt ccagaagta cttatggtg 
      gaagaagta tttaacaac gttgcaaat ggttcaatt 
      tgtggcgat catggtcca tgccttcca gttactgtt 
      caccgattg caactaccc tcgtgcctt cgtctggtg 
      gattgggat gcacgctgt catgttctt ctttctgtt 
      taaggagtt ctacatcca aacttataa taaaagaac 
      agcagcagc ggcggcggc ggcggcggc ggcggcagc 
      gaagaacgg cgtcgtcca gaacggcac aggtgcgca 
      tcagaacgg agttaaatc gaacaaagt tagccaata 
      ttatgtcaa cggaacggc ggacttgca tcaaaggat 
      aaagtcgca gtgatatag tggtagctt tatttattc 
      aattgagtc atgatcgaa ctgtttact taatttaat 
      tctgtagga taattattc cgattcatg actataaag 
      caatttgct caatttttt agcacttct ccattcact 
      cgaatcata catttatta ggattgtac aaataagaa 
      gaatttttg tagccacta acaaatacg tgcctatgt 
      cagggtgta gtggaattg aaatttttc gttggagct 
      ttcaggcga atgtattcc attatgttt ttaacaaaa 
      attaattgc aataattat atcaagcgt ttttgttag 
      atctgtgtg ttcatactt gattctaaa actttttag 
      caataaaat atgttttat atattttag gtgctgttt 
      ttaaacgtt attgtaaaa attaatttg ttatcattt 
      tatattatt taaagaaaa tcactcaaa tatacttac 
      ttagatggt cttacatag atgggattt taaaggatt 
      gttgaacat tgaaattat gcattttat gtagattaa 
      atcaggtta tttgttgta cctatgtat tttttatat 
      ttactatta ttgaaataa aaatgtcta aaaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY