Status
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Gene information
General information
TC010951 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1866297..1875583
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010951
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01768

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010951 RA
      MEIEQTFQD FKDNAVQEI FTDCMSVEI 
      NSRSQSMML LLPGAISEL TDNLVLTSA 
      SSVNTNVLD ALNITCVIN VAPELPDTP 
      LHRSDIIYH KIPLLDSGN SRIYTHFDE 
      AADLIHKVA NSGGKTLIY CVAGVSRSA 
      SICLAYLMK HQGLTLLEA YNYVKLRRP 
      KIKPNCGFF KQLIEYEKD IFGDNTVRM 
      IFNEFVQME IPDIYDCDY QFVSHFSKK 
      KRNQRY
    CDS sequence
    • >TC010951 RA
      atggaaatc gaacagacc ttccaggat tttaaggat 
      aacgcagtc caggaaatc ttcacggac tgcatgagt 
      gtcgagatc aattcaaga tcgcagtcg atgatgttg 
      ttactccct ggtgccatt tcggaattg accgacaat 
      ttagtcctg acttcggct tcatctgtc aacactaat 
      gtccttgat gcactcaat atcacttgc gttataaat 
      gtggcgcct gaattgccg gacacgcca ctgcacaga 
      agcgacatc atttatcac aagattcct cttttggat 
      tcggggaat tccaggatt tacacgcat tttgatgaa 
      gctgccgac ttgatacac aaagttgct aattcaggg 
      ggaaaaact ttgatttat tgtgttgct ggtgtcagt 
      agatcggca agtatttgc ttggcctat ctcatgaaa 
      catcaagga ttaacactt ttggaagcc tacaactat 
      gtcaagtta aggagacct aaaattaag ccaaactgc 
      ggatttttc aagcaactg atagaatat gaaaaggac 
      atttttggg gataatacc gtcaggatg attttcaat 
      gagttcgtc caaatggag ataccggac atctacgac 
      tgcgactac cagtttgtc agtcatttc agcaagaag 
      aaacgaaac caaagatat taa
    mRNA sequence
    • >TC010951 RA
      attaattat atgtgtatt aaaaattga aaaaagaga 
      gtagcagga atcggcgtc tatggttta aaaacagtt 
      taaaaaaaa gaacagttt tgtatcatc aggtgtagt 
      aatcaataa acagcttgt ttatctaca agtggaaag 
      attacttaa gttcagaag tgactaagt cactagtgc 
      acttattgc acgagcgga taatctttg tgtataata 
      caagatttt ttctataac tgaacttta agcttaagt 
      gaaagaagt tattgttaa tccttcaaa ttaatgttg 
      gaatttgta aatttaatt gaataaaat aaatttaaa 
      acatctacc tttgcgagt aagagttat tataaagaa 
      atggtttaa cagctggaa aaatatata tttttttgt 
      gagaaacag gaagtaaca gcaacgttt cttaaagtt 
      tagtcttta agaatttga ctttgtatt aatgcacac 
      taacactga acacttcgc atttactat ttacttata 
      gtactgagc attaaaatc tctgatgca aattcaatt 
      ttcgttcgt tttagaaat ttgattaaa aaatgtaaa 
      tttttgtta tttttgtga ttctgtgat tgggacaac 
      aaacatgac cttgtcacg tgccatcct tgccccgac 
      aaattttgg ttttgtgat tgccaatga ccgagttcc 
      gctttaaaa taatttcag gacgtgtct cagcttccc 
      cagtagttc tgcgtattt gaaaatatg cttaatttt 
      agaacaata cagtctgaa atgtgcttt gtgagcaca 
      ccacctggc caagtaatt gtcaaaaat ttcctcggg 
      tctttattt agactcgct tcagcaaaa ataaaaatt 
      caagttcac caaatcctg aaaatcacc tgaaatccc 
      actcggctc cgatgactg atccacgcc cattcatca 
      atctattat aacaacagt ttttcgctt atgaatgaa 
      ccgattgaa ccggctaaa aatatgata gttctcgtt 
      tcacatact tgtgcgtcg tcgacgtcg cgacgtttc 
      aggccggct gaaaatata taaggatgg aatgtagtg 
      tttacacaa atatttggc gatggaatg tcgcttttc 
      aggcgcgca tgtccgctt tattttgat tcggtttaa 
      aacctcagg taatcgcgg gattctatg actcagttt 
      gtttggcag cggttcagt ggtttgttt cgaaaagaa 
      cactttcac tcaaaaatt ttgaatttt tcaccgagg 
      aatggaaat cgaacagac cttccagga ttttaagga 
      taacgcagt ccaggaaat cttcacgga ctgcatgag 
      tgtcgagat caattcaag atcgcagtc gatgatgtt 
      gttactccc tggtgccat ttcggaatt gaccgacaa 
      tttagtcct gacttcggc ttcatctgt caacactaa 
      tgtccttga tgcactcaa tatcacttg cgttataaa 
      tgtggcgcc tgaattgcc ggacacgcc actgcacag 
      aagcgacat catttatca caagattcc tcttttgga 
      ttcggggaa ttccaggat ttacacgca ttttgatga 
      agctgccga cttgataca caaagttgc taattcagg 
      gggaaaaac tttgattta ttgtgttgc tggtgtcag 
      tagatcggc aagtatttg cttggccta tctcatgaa 
      acatcaagg attaacact tttggaagc ctacaacta 
      tgtcaagtt aaggagacc taaaattaa gccaaactg 
      cggattttt caagcaact gatagaata tgaaaagga 
      catttttgg ggataatac cgtcaggat gattttcaa 
      tgagttcgt ccaaatgga gataccgga catctacga 
      ctgcgacta ccagtttgt cagtcattt cagcaagaa 
      gaaacgaaa ccaaagata ttaagtttt ggttgatta 
      ttccagagt gcattttac cacagaaaa cgacatcat 
      attgatgtc ggattgtaa ctaaattac gaaggtgtt 
      ttattaatt gaacaaata ttaggtacg agattaatt 
      cagatacca tctgctggt gtttgcaag tgtttaaca 
      tactgaaac tgtaattta tttgtatct ttattgtgt 
      ctggattaa tctaattac ataacaatg tttgtaaca 
      acatgactt attttgttt tttttgtac tgataattt 
      ataagggac caagtttat ttatcgtgt tttatgtac 
      atagttttt atttaataa aaaactgat tcaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY