Status
Please wait ...
Gene information
General information
TC010919 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1263640..1282177
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010919
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01764

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Fertility
    mature egg deposition >5 number 2nd lateral oviduct: 5 number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    vitellarium mostly decreased - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 4

    tropharium shortened - Number of animals showing the phenotype: 4

    egg chamber mostly not present - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010919 RA
      MELTGLICE MSLCRLCGE ENVNGTELF PTRENEPDL 
      SELVNKYLP LKIENDRKF PTKICPGCN IQLEATKSF 
      MDLIVQGQI KLRQLYKLQ QDKLNRQEK QRLQLEEAL 
      KSVNPNSSV DTYTIQSDE TGEKFLIQI FSHGPLFPP 
      EHELSLKAE GLTRPRRKR GRPPKPPNK EDQSLKTND 
      KDICKVEEK LEQFEEATE DGKRKRRIK VPTRYSEAI 
      QGKELDKVF KQEGVIDEE SVSDEDDPI LDEGEGSNK 
      VEEVIGRLE TENGEDLGQ PVIINKTLN KTRPRNKSI 
      AGQSKRRRK YQCEICGRE FLHHGRYEL HKKMHEVEY 
      SCTQEKCTF VTDQKPLLE QHQEENGHS GHTVSEKVD 
      KYGTLQLPT DLKPLQAPN PDQPPPAPA PKKEFTCEQ 
      CLKPFSCKQ NYEVHLKAV HAGERPFAC ELCNKRFSY 
      ANSLKVHLL QHFPKTGPD DDSSAQYKC PSCPKSFRH 
      PSSLQYHRD SEHTNGRRF VCNKCDKPF KHRQLLQRH 
      QLVHSDERP HRCSQCNSA FKTRANLIH HTRTVHSGQ 
      RSHWCSQCD KAFAHKTAL KLHQRWHSG VRPYQCEFC 
      KKSFSQKGN LAEHRRIHT GEKPYQCDH CGRAFTTSS 
      QFRLHKKRH LDERPHRCE FCQKSFLHK VTLRCHMRR 
      HFDERPFKC QQCPKTFPE AWALKKHER LHTGEKPYK 
      CDLCSKAFA DSSNLAKHR RTHRNLKDK QMLFVTYEK 
      DGGEAIVTY IEPQDTVVE TNEIINVAE EIDDLKTIQ 
      LQQLVDQEG NPISFITQD GQQVKVVAS SEDGQENIQ 
      GLLPDGTLV PISLTTVPE KTKPIVTQI EETQKIDVP 
      IEGQLLGSN IQFLSDDDP KVDNNIQFV AEDNQNMCL 
      VTTYNIEDS INPQYLSMP
    CDS sequence
    • >TC010919 RA
      atggagttg acaggcttg atatgtgaa atgtctctt 
      tgtcgattg tgcggtgag gaaaatgtg aacgggacg 
      gaattgttt ccaacaaga gaaaacgag ccggattta 
      agtgaactt gttaacaaa tatttaccg ttgaagatc 
      gagaatgac cgaaagttt ccaacgaaa atctgcccg 
      ggctgcaac attcagctg gaagcgacc aaatcgttc 
      atggacctg atagtccaa ggccaaatt aagctgaga 
      cagctgtat aaattgcaa caagacaag ttgaaccgg 
      caggaaaag caaagattg caactagaa gaggcgttg 
      aaaagtgtt aatcccaac tccagcgtc gatacttac 
      accattcag tcggatgag actggagag aaatttcta 
      atacaaatt ttttcacac ggacctttg tttcctccc 
      gaacacgaa ttatcatta aaggccgaa ggcctcact 
      agacctcga agaaaacgt ggtagaccc ccaaaaccg 
      cctaataaa gaagaccaa tccttaaaa acaaacgac 
      aaagatatt tgtaaagtt gaagaaaaa ttggagcaa 
      tttgaagaa gccacagaa gacgggaaa agaaaacga 
      agaattaag gtccccacc cgatactct gaagcgatt 
      caagggaag gaattggat aaagtgttt aaacaagag 
      ggtgttata gacgaagaa tcggtctca gacgaggac 
      gatccgatt ttagacgaa ggcgaaggc tcaaataag 
      gttgaggaa gtaatagga cgtctggag accgaaaac 
      ggtgaagat ttaggccaa ccagtcata ataaataag 
      actttgaat aaaacacgt cctagaaat aaaagtata 
      gcaggccag tcaaaacgt cgacgaaaa taccaatgt 
      gagatttgc ggacgcgaa tttttacac catgggagg 
      tacgaattg cataaaaaa atgcacgag gtggagtac 
      agctgcacc caagaaaag tgtactttt gtgaccgat 
      caaaagccg cttctggag caacaccag gaggaaaat 
      ggtcattct ggtcacaca gtttcggaa aaagttgat 
      aaatatgga actctccag ctgcccacc gacctcaaa 
      cccctccaa gcaccaaac cccgatcag ccgccccca 
      gcccccgcc cccaaaaag gagttcacg tgcgagcaa 
      tgcctcaag cccttctcc tgcaaacaa aactacgaa 
      gtacacctc aaggccgtc catgccggt gagcgcccc 
      ttcgcttgc gaactctgc aacaagcgc ttttcgtac 
      gccaacagc ctcaaagtc caccttctg caacacttc 
      cccaaaaca ggtcccgac gacgactcc agcgcccag 
      tacaagtgc ccctcatgc cctaagagc ttccggcat 
      ccgagcagt ctgcagtac caccgcgac tcggagcac 
      accaacgga cgccgcttc gtctgcaac aagtgcgac 
      aagccgttc aagcatcgc cagctgctg cagcgccac 
      cagctggtg cactccgac gagcgcccg catcgatgc 
      tcgcagtgc aattcggcg ttcaaaacg cgcgctaat 
      ctcatccac cacacgagg acggtgcat tcgggacaa 
      cgcagtcat tggtgctcg cagtgcgat aaggccttt 
      gcgcacaaa accgcactc aagttgcac cagaggtgg 
      cattcgggg gtaaggccc taccagtgc gagttctgc 
      aagaaatcc ttctcacag aaggggaat ttggcagag 
      cacaggaga atacacact ggggaaaaa ccctaccag 
      tgtgaccat tgtggaagg gcgttcacc acctcatcg 
      cagtttaga cttcacaag aaacgacat ttggatgag 
      agaccacat aggtgcgag ttttgtcaa aagagtttt 
      ctacataag gtcactttg aggtgtcac atgagacgg 
      cattttgat gagaggcct ttcaagtgt cagcaatgc 
      cccaaaacg ttccctgaa gcttgggct ttgaaaaaa 
      cacgagaga ctacacacc ggggaaaaa ccctacaag 
      tgtgacttg tgctccaaa gccttcgcg gacagctcg 
      aacttggcc aaacatcga cggactcac agaaatttg 
      aaagacaaa cagatgctc tttgtaacc tatgaaaaa 
      gacggaggt gaggcgatt gttacgtat atagaacca 
      caagataca gtagtcgaa acaaacgaa ataataaac 
      gttgctgaa gaaattgac gatttgaaa acaatacaa 
      ttgcaacaa cttgtggac caggaaggt aacccaatt 
      agttttata acgcaagat gggcaacag gttaaggtt 
      gtggcaagt agcgaagat gggcaagaa aatatccaa 
      gggttactc cctgatggt acgttggta ccaattagt 
      ttgacaacg gtaccagaa aaaactaaa ccaatagta 
      acgcaaatc gaggagaca caaaagata gatgtgcca 
      attgagggc cagttatta ggaagcaac atccaattc 
      ctctccgat gatgaccca aaagtggac aataatatt 
      caattcgta gctgaagat aatcaaaac atgtgctta 
      gtgacaact tacaacatt gaagattct ataaatccg 
      caatatctt agtatgccc taa
    mRNA sequence
    • >TC010919 RA
      tttcccgac gttggccga aaacctgga aacaaaatg 
      gagttgaca ggcttgata tgtgaaatg tctctttgt 
      cgattgtgc ggtgaggaa aatgtgaac gggacggaa 
      ttgtttcca acaagagaa aacgagccg gatttaagt 
      gaacttgtt aacaaatat ttaccgttg aagatcgag 
      aatgaccga aagtttcca acgaaaatc tgcccgggc 
      tgcaacatt cagctggaa gcgaccaaa tcgttcatg 
      gacctgata gtccaaggc caaattaag ctgagacag 
      ctgtataaa ttgcaacaa gacaagttg aaccggcag 
      gaaaagcaa agattgcaa ctagaagag gcgttgaaa 
      agtgttaat cccaactcc agcgtcgat acttacacc 
      attcagtcg gatgagact ggagagaaa tttctaata 
      caaattttt tcacacgga cctttgttt cctcccgaa 
      cacgaatta tcattaaag gccgaaggc ctcactaga 
      cctcgaaga aaacgtggt agaccccca aaaccgcct 
      aataaagaa gaccaatcc ttaaaaaca aacgacaaa 
      gatatttgt aaagttgaa gaaaaattg gagcaattt 
      gaagaagcc acagaagac gggaaaaga aaacgaaga 
      attaaggtc cccacccga tactctgaa gcgattcaa 
      gggaaggaa ttggataaa gtgtttaaa caagagggt 
      gttatagac gaagaatcg gtctcagac gaggacgat 
      ccgatttta gacgaaggc gaaggctca aataaggtt 
      gaggaagta ataggacgt ctggagacc gaaaacggt 
      gaagattta ggccaacca gtcataata aataagact 
      ttgaataaa acacgtcct agaaataaa agtatagca 
      ggccagtca aaacgtcga cgaaaatac caatgtgag 
      atttgcgga cgcgaattt ttacaccat gggaggtac 
      gaattgcat aaaaaaatg cacgaggtg gagtacagc 
      tgcacccaa gaaaagtgt acttttgtg accgatcaa 
      aagccgctt ctggagcaa caccaggag gaaaatggt 
      cattctggt cacacagtt tcggaaaaa gttgataaa 
      tatggaact ctccagctg cccaccgac ctcaaaccc 
      ctccaagca ccaaacccc gatcagccg cccccagcc 
      cccgccccc aaaaaggag ttcacgtgc gagcaatgc 
      ctcaagccc ttctcctgc aaacaaaac tacgaagta 
      cacctcaag gccgtccat gccggtgag cgccccttc 
      gcttgcgaa ctctgcaac aagcgcttt tcgtacgcc 
      aacagcctc aaagtccac cttctgcaa cacttcccc 
      aaaacaggt cccgacgac gactccagc gcccagtac 
      aagtgcccc tcatgccct aagagcttc cggcatccg 
      agcagtctg cagtaccac cgcgactcg gagcacacc 
      aacggacgc cgcttcgtc tgcaacaag tgcgacaag 
      ccgttcaag catcgccag ctgctgcag cgccaccag 
      ctggtgcac tccgacgag cgcccgcat cgatgctcg 
      cagtgcaat tcggcgttc aaaacgcgc gctaatctc 
      atccaccac acgaggacg gtgcattcg ggacaacgc 
      agtcattgg tgctcgcag tgcgataag gcctttgcg 
      cacaaaacc gcactcaag ttgcaccag aggtggcat 
      tcgggggta aggccctac cagtgcgag ttctgcaag 
      aaatccttc tcacagaag gggaatttg gcagagcac 
      aggagaata cacactggg gaaaaaccc taccagtgt 
      gaccattgt ggaagggcg ttcaccacc tcatcgcag 
      tttagactt cacaagaaa cgacatttg gatgagaga 
      ccacatagg tgcgagttt tgtcaaaag agttttcta 
      cataaggtc actttgagg tgtcacatg agacggcat 
      tttgatgag aggcctttc aagtgtcag caatgcccc 
      aaaacgttc cctgaagct tgggctttg aaaaaacac 
      gagagacta cacaccggg gaaaaaccc tacaagtgt 
      gacttgtgc tccaaagcc ttcgcggac agctcgaac 
      ttggccaaa catcgacgg actcacaga aatttgaaa 
      gacaaacag atgctcttt gtaacctat gaaaaagac 
      ggaggtgag gcgattgtt acgtatata gaaccacaa 
      gatacagta gtcgaaaca aacgaaata ataaacgtt 
      gctgaagaa attgacgat ttgaaaaca atacaattg 
      caacaactt gtggaccag gaaggtaac ccaattagt 
      tttataacg caagatggg caacaggtt aaggttgtg 
      gcaagtagc gaagatggg caagaaaat atccaaggg 
      ttactccct gatggtacg ttggtacca attagtttg 
      acaacggta ccagaaaaa actaaacca atagtaacg 
      caaatcgag gagacacaa aagatagat gtgccaatt 
      gagggccag ttattagga agcaacatc caattcctc 
      tccgatgat gacccaaaa gtggacaat aatattcaa 
      ttcgtagct gaagataat caaaacatg tgcttagtg 
      acaacttac aacattgaa gattctata aatccgcaa 
      tatcttagt atgccctaa ggagcaaac ctgaaattt 
      atgtgtgta ttttatatg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY