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Gene information
General information
TC010917 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1282183..1288378
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010917
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01763

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi) mature egg deposition >5 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010917 RA
      MQNVINSVK GTALGVAEY LTPVLKESK FRETGVLTP 
      EEFVAAGDH LVHHCPTWQ WAAGDESKA KPYLPKDKQ 
      FLVTRNVPC TRRCEQMEY SEDLERIIE SDDADNGWV 
      DTHHYDQDQ TTAVVEEKI SEMTLSNSK GESMEEAAL 
      NDEENDDED DDDDEAADM EAFEESGML DDAQATTVI 
      QPKVTAKKE LDEDGEIIH TRTYDLHIT YDKYYQTPR 
      LWLTGYNEH HKPLTVEEL YQDVSKDYA MKTVTMETH 
      PHLSVPKMA SVHPCRHAE VMKSIIETV TEGGGELGV 
      HMYLIIFLK FMQSVIPTI EYDYTQNFT M
    CDS sequence
    • >TC010917 RA
      atgcagaac gtgatcaac agtgtgaaa ggtacagcc 
      cttggtgtt gccgaatat ctaacccca gttttaaag 
      gaatcgaaa tttagagaa actggggtt ctaacccct 
      gaagaattc gttgcggct ggtgaccac ctcgtgcac 
      cattgcccc acttggcaa tgggcagct ggggatgaa 
      agcaaagct aaaccctac ttacctaaa gacaagcag 
      tttctggtc acaagaaat gtaccgtgc acgcgtagg 
      tgcgaacaa atggaatac tctgaagat ttggaacga 
      attattgaa tcagatgat gctgataat ggttgggtt 
      gacacccat cactacgat caagaccaa acaacggcc 
      gttgttgaa gagaaaatt tcggaaatg actctcagc 
      aactccaag ggggagagt atggaagaa gcagctcta 
      aatgatgag gaaaacgac gatgaggac gatgatgat 
      gatgaggcc gcagatatg gaagctttt gaagagagc 
      ggaatgcta gatgatgca caggcgact acagtgatt 
      cagccaaaa gtaacagct aaaaaagaa ttggatgaa 
      gatggtgaa ataatccac acacgcaca tatgatttg 
      catattact tatgataag tattaccaa actccgaga 
      ttgtggctt actggttac aatgagcat cacaaaccc 
      ctcactgtt gaagagttg tatcaggat gtgagcaaa 
      gattacgcg atgaaaacc gttacaatg gagacgcat 
      ccgcatcta agtgtaccc aaaatggcc tcagtccat 
      ccctgcaga cacgctgag gtcatgaag agcatcatc 
      gagactgta actgaaggc ggcggtgag ttaggagtc 
      cacatgtac ctaataata ttcctcaaa ttcatgcaa 
      tcggtcatc ccaactata gaatacgat tatactcaa 
      aacttcacg atgtaa
    mRNA sequence
    • >TC010917 RA
      caaccatta aatcagaat tgttaattg taaagtcta 
      gaagcgatc gataaccta aaacatgga gttcaaagt 
      tttgtttcg caatttttg aaaaagtcg tccctattt 
      ccaaatcga aatttccag taatagccc aaaaatgca 
      gaacgtgat caacagtgt gaaaggtac agcccttgg 
      tgttgccga atatctaac cccagtttt aaaggaatc 
      gaaatttag agaaactgg ggttctaac ccctgaaga 
      attcgttgc ggctggtga ccacctcgt gcaccattg 
      ccccacttg gcaatgggc agctgggga tgaaagcaa 
      agctaaacc ctacttacc taaagacaa gcagtttct 
      ggtcacaag aaatgtacc gtgcacgcg taggtgcga 
      acaaatgga atactctga agatttgga acgaattat 
      tgaatcaga tgatgctga taatggttg ggttgacac 
      ccatcacta cgatcaaga ccaaacaac ggccgttgt 
      tgaagagaa aatttcgga aatgactct cagcaactc 
      caaggggga gagtatgga agaagcagc tctaaatga 
      tgaggaaaa cgacgatga ggacgatga tgatgatga 
      ggccgcaga tatggaagc ttttgaaga gagcggaat 
      gctagatga tgcacaggc gactacagt gattcagcc 
      aaaagtaac agctaaaaa agaattgga tgaagatgg 
      tgaaataat ccacacacg cacatatga tttgcatat 
      tacttatga taagtatta ccaaactcc gagattgtg 
      gcttactgg ttacaatga gcatcacaa acccctcac 
      tgttgaaga gttgtatca ggatgtgag caaagatta 
      cgcgatgaa aaccgttac aatggagac gcatccgca 
      tctaagtgt acccaaaat ggcctcagt ccatccctg 
      cagacacgc tgaggtcat gaagagcat catcgagac 
      tgtaactga aggcggcgg tgagttagg agtccacat 
      gtacctaat aatattcct caaattcat gcaatcggt 
      catcccaac tatagaata cgattatac tcaaaactt 
      cacgatgta aagggagtt gttagcttc aatttactc 
      actgtgctt tgtattgtt atgtttatt attaaatcg 
      gcggttttt ttaattata tttttctat taaaatgct 
      tgtgtaata aatatacat cttcaaaat aaattatac 
      aggctcct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY