Status
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Gene information
General information
TC010914 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1328465..1338798
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010914
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01762

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 4

    wings blistered - Number of animals showing the phenotype: 4

    longitudinal dorsal thoracic muscles mostly not present - Number of animals showing the phenotype: 4

    Comment: sometimes attachment point visible, but never 3 on one side
    oblique dorsal thoracic muscles thinner - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010914 RA
      MSSDEEKPP APPVRLTSN RGTDILSNS LAMDRPLPK 
      EPDDADRKK KNLKSKKAS KLSTYTSDK PNISYPTNF 
      EHTVHVGFD PVSGEFTGM PEAWSRLLM NSNISKQEQ 
      KNNPQAVLD VLTWFDNTT KEPPNTKYM TKTTTATHS 
      GSSLSRVSS SSPSSTTPT EAEGGSHSY HSPAHAASS 
      EIEDEAPPP PIASRPERT KSIYTKPIE EVQITNNSV 
      GTPTHSLSP AHTTPTHGA NANSTPPAP ILDRNKNQA 
      GPEMSRAAS EKRKKKMSD EEILEKLRS IVSVGDPIR 
      KYTKIDKIG QGASGTVYT AMETSTGVE VAIKQMNLS 
      QQPKKELII NEILVMREN KHGNVVNYL DSYLVNEEL 
      WVVMEYLPG GSLTDVVTE TCMDEGQIA AVCREVLQA 
      LDFLHSNQV IHRDIKSDN ILLGLDGSV KLTDFGFCA 
      QISPEQSKR TTMVGTPYW MAPEVVTRK QYGPKVDVW 
      SLGIMAIEM IEGEPPYLN ENPLRALYL IATNGKPDI 
      KDKEKLSPS FQDFLDQCL AVEVDKRAS ARDLLKHPF 
      LKLARPLAS LTPLILAAK EAAKQH
    CDS sequence
    • >TC010914 RA
      atgtcttct gacgaggag aagcctccg gcgcccccc 
      gtgcgcctc acctccaac cggggcacc gacatcctc 
      tccaactcg ctggcgatg gaccggccc ctgcccaag 
      gagcccgac gacgccgac cgcaagaag aagaacctc 
      aagtcgaag aaagcgagc aaattgtcc acgtacacc 
      tccgacaag cccaacatc tcctatccc accaacttc 
      gagcacacg gtccatgtg ggcttcgac cccgtctcc 
      ggagagttc acgggaatg ccggaagca tggtctcga 
      ctgttaatg aactccaac atcagcaaa caggaacaa 
      aagaacaac ccccaggcc gttctcgat gttctcact 
      tggtttgat aatacaaca aaagaaccg ccaaacact 
      aaatacatg acgaaaacc acaacagca acacattca 
      ggttcgtca ttgagccgc gtttccagt tcgagtccg 
      agcagcaca acgccgacg gaggccgaa gggggctca 
      cattcctac cacagtcca gcgcacgcc gccagttcc 
      gaaatcgaa gacgaagcg cctccgcca cctatcgcc 
      agtcgcccc gaacgcacc aaaagcatt tacacaaaa 
      ccaatcgaa gaggttcag ataacaaat aacagcgtc 
      gggacgccg acccacagc ctgagtccg gcccatacg 
      acgccgaca catggagcc aacgcgaat tcgacgccg 
      ccggcgccc atcctcgat cgaaacaag aaccaagcc 
      ggtcctgaa atgtcaaga gcagcgtcg gaaaaacga 
      aagaaaaaa atgagcgat gaagaaatc ttggaaaaa 
      ttgagaagt attgtttcg gtcggtgat ccaatcaga 
      aaatacacc aaaatcgat aagattggt caaggtgct 
      tcgggaact gtttacacg gcgatggaa acgtccaca 
      ggtgtagaa gttgccatc aaacaaatg aatttatca 
      caacagcct aagaaagaa ctgattatc aatgagatt 
      ctagtcatg cgagaaaac aaacacgga aatgtagtg 
      aattatcta gacagttat cttgtgaac gaggaactc 
      tgggttgtg atggaatat ttaccaggc ggttcactg 
      actgacgta gtaacggag acgtgcatg gacgagggt 
      caaatcgcg gctgtgtgt cgagaagtc cttcaagcg 
      ctcgacttt ctccattca aaccaagtc atccatagg 
      gacattaaa tcggacaat attctcctc ggacttgac 
      ggctccgtc aaattaact gatttcggt ttctgtgct 
      caaataagt cccgaacaa tcaaaaaga acgacgatg 
      gtggggacg ccttattgg atggcgccg gaagtcgtc 
      acgcggaag caatacggc cctaaggtc gatgtgtgg 
      tcgttgggc atcatggcg atcgaaatg atcgaaggt 
      gagccgccg tatttgaac gaaaatccg ctgagggcg 
      ctgtatttg attgcaacc aacggcaaa cccgatatt 
      aaagataag gagaagttg tcgccctcc tttcaagat 
      tttctagac caatgtttg gctgtggaa gtcgacaaa 
      agggcgtcg gcgcgcgat ttattaaaa catcccttc 
      ttaaagttg gcacgaccg ttggccagc ctgacgccg 
      ctcattttg gcggcgaaa gaagcggca aaacaacac 
      tag
    mRNA sequence
    • >TC010914 RA
      ggtgtcagc aagatggtg gtcgttcca aaagtaatc 
      ggtgattag atatggatt ttgtagata ttcctcact 
      aaatgtgct tagttattc tgtgaattt gcaagtgtg 
      tgattgttg tgagtgttc tgcatcgac taaaccagt 
      gcctgtcac accttaagg atgtcttct gacgaggag 
      aagcctccg gcgcccccc gtgcgcctc acctccaac 
      cggggcacc gacatcctc tccaactcg ctggcgatg 
      gaccggccc ctgcccaag gagcccgac gacgccgac 
      cgcaagaag aagaacctc aagtcgaag aaagcgagc 
      aaattgtcc acgtacacc tccgacaag cccaacatc 
      tcctatccc accaacttc gagcacacg gtccatgtg 
      ggcttcgac cccgtctcc ggagagttc acgggaatg 
      ccggaagca tggtctcga ctgttaatg aactccaac 
      atcagcaaa caggaacaa aagaacaac ccccaggcc 
      gttctcgat gttctcact tggtttgat aatacaaca 
      aaagaaccg ccaaacact aaatacatg acgaaaacc 
      acaacagca acacattca ggttcgtca ttgagccgc 
      gtttccagt tcgagtccg agcagcaca acgccgacg 
      gaggccgaa gggggctca cattcctac cacagtcca 
      gcgcacgcc gccagttcc gaaatcgaa gacgaagcg 
      cctccgcca cctatcgcc agtcgcccc gaacgcacc 
      aaaagcatt tacacaaaa ccaatcgaa gaggttcag 
      ataacaaat aacagcgtc gggacgccg acccacagc 
      ctgagtccg gcccatacg acgccgaca catggagcc 
      aacgcgaat tcgacgccg ccggcgccc atcctcgat 
      cgaaacaag aaccaagcc ggtcctgaa atgtcaaga 
      gcagcgtcg gaaaaacga aagaaaaaa atgagcgat 
      gaagaaatc ttggaaaaa ttgagaagt attgtttcg 
      gtcggtgat ccaatcaga aaatacacc aaaatcgat 
      aagattggt caaggtgct tcgggaact gtttacacg 
      gcgatggaa acgtccaca ggtgtagaa gttgccatc 
      aaacaaatg aatttatca caacagcct aagaaagaa 
      ctgattatc aatgagatt ctagtcatg cgagaaaac 
      aaacacgga aatgtagtg aattatcta gacagttat 
      cttgtgaac gaggaactc tgggttgtg atggaatat 
      ttaccaggc ggttcactg actgacgta gtaacggag 
      acgtgcatg gacgagggt caaatcgcg gctgtgtgt 
      cgagaagtc cttcaagcg ctcgacttt ctccattca 
      aaccaagtc atccatagg gacattaaa tcggacaat 
      attctcctc ggacttgac ggctccgtc aaattaact 
      gatttcggt ttctgtgct caaataagt cccgaacaa 
      tcaaaaaga acgacgatg gtggggacg ccttattgg 
      atggcgccg gaagtcgtc acgcggaag caatacggc 
      cctaaggtc gatgtgtgg tcgttgggc atcatggcg 
      atcgaaatg atcgaaggt gagccgccg tatttgaac 
      gaaaatccg ctgagggcg ctgtatttg attgcaacc 
      aacggcaaa cccgatatt aaagataag gagaagttg 
      tcgccctcc tttcaagat tttctagac caatgtttg 
      gctgtggaa gtcgacaaa agggcgtcg gcgcgcgat 
      ttattaaaa catcccttc ttaaagttg gcacgaccg 
      ttggccagc ctgacgccg ctcattttg gcggcgaaa 
      gaagcggca aaacaacac tagcgataa gtttcaggc 
      ttttgtcct gttcgttat caattttct tttgtatta 
      tatatacgt gagagacta tattcccat gttactaga 
      aatgcattc gattttttt aaaacaata atgtaacat 
      aatcgtata ttttattat tttagcttg cagttctag 
      ttatatggg tcgtatttt attagggac gtaagcatt 
      taatagagg tctgatgtt cgtataagt gacaggaga 
      cgtgtgcct aatttcaca acaataacg ctgcgtagg 
      gatttttcg tttcgttgt cactagctt ctttttatg 
      cttttgcgc ttgattgat cgaaaatgc gattttttg 
      tagctacag ggtagttcc tgttttcgg ttttattcc 
      tctataaat tctatgacc agtctttgt attaaaaaa 
      gtatcattt tataatcga aattattta ttattttgt 
      taacgtttt agtttcatc tatattttg ttaagaaga 
      aaaacttgt aaagaaata ctaggaaaa gctatattg 
      taaaacaaa cgcctgtca aaggtctat atagatgaa 
      gaatgagtg ttagtctgg atgatttaa ctagttata 
      gtatttttg cactagtat ttatcaaag aaaaattca 
      tattgtgat tgccaaggt atattcgaa ggcatattg 
      ttgccaatg aaagattgt gtatggtaa ttaaaataa 
      aatcatttt ataatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY