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Gene information
General information
TC010912 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1371841..1373298
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010912
    iBeetle screen iB_01761

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010912 RA
      MNPKSLFLL VFVTILGVT SSEVSFKNV TLWTWEKNI 
      NTIDSANNL GTYLPKKWT STIIVIGTI PILHENSVT 
      NVENVKELQ FLKNQIVEI RPGAFKNLN SVKTLKING 
      SNITTIAGV FDNLTLEQL NLNNNQISV IPKGVVKNP 
      NLKGLSFAG NLIETIEES AFDHLPNLE TLDLFQNLL 
      SDLPLGIFK HLPHLTIVS LSNNKFTTI KEEIFDSSS 
      IRYLYLDGN LLKTVPENF MGRMPNLQG IYLNRNHLK 
      TIPAGIFNN KTLLHIELA NNEISHIAP DAFNNMPNL 
      ANLILSSNK LAQYNNNWL RNCPNIYRL FLSNNLIEQ 
      LPVQAFKNI YNQKEQWVK LNSNKIKKI TAENFKGWK 
      LVDTLDLSQ NEIEQWHED YLADAEFIR ALNLAYNKI 
      TCLDGDLDK IFKAKKATY LSGNPLSEE CKSKIREWS 
      ARNQDKVSL
    CDS sequence
    • >TC010912 RA
      atgaatccg aaatcactg tttttgcta gtttttgtg 
      actattttg ggtgtaaca tccagtgaa gtgtcattc 
      aaaaatgtt accctttgg acgtgggag aaaaatatc 
      aacaccatc gattccgca aacaatctc gggacttat 
      ttacctaaa aagtggact tcgacaatt attgtaatc 
      ggaacaata ccaatttta catgaaaat tccgtcaca 
      aacgttgaa aatgtgaag gaacttcaa ttcctcaaa 
      aatcaaatc gttgaaata cgtccaggt gcgttcaaa 
      aatttaaac agtgtgaaa acattgaaa attaatgga 
      agcaacatc acaaccatc gctggagtt tttgataat 
      ctcacttta gagcagctc aacttaaat aacaaccaa 
      atctcggta atcccaaaa ggagttgtg aaaaatccc 
      aatttgaaa ggactgagc tttgccggt aatttaatc 
      gaaactatt gaagaaagt gcttttgac catcttcca 
      aacttggaa acgttggat ttatttcaa aatttactc 
      tcagatctt ccactcgga attttcaaa catttaccc 
      catctcacg attgtttcg ttaagcaat aataaattc 
      accacaatc aaggaagaa attttcgac agctcgtca 
      atacgttac ctatatttg gatggtaat ttactgaaa 
      accgttcca gaaaatttc atgggccga atgccaaat 
      ttacaggga atttaccta aatcgaaac cacttaaaa 
      acaattcct gctggcatc ttcaataac aaaactttg 
      cttcacata gagctagcc aataacgaa atttcccac 
      attgcacca gatgcattc aacaacatg cccaacctg 
      gcaaattta attttgtcg agtaataaa ttggcgcaa 
      tataacaac aactggttg cgtaattgt ccgaacatc 
      tatcgccta tttctttcc aataatctc atcgagcaa 
      cttccagtc caagcattt aaaaatatt tacaaccaa 
      aaagaacag tgggttaag ttaaacagc aacaaaatt 
      aagaaaatt acagctgaa aatttcaaa ggatggaaa 
      ctagtcgat accttggat ctgtctcag aatgaaata 
      gaacaatgg catgaggat tatcttgca gatgctgaa 
      tttatccgg gctctcaac cttgcatat aacaagatt 
      acgtgtctg gacggtgat ttagataaa atttttaaa 
      gccaaaaaa gccacttat ttaagtggc aatccgttg 
      agtgaagag tgtaaaagc aaaattcgg gaatggtcc 
      gcaagaaat caagacaaa gtcagtttg tga
    mRNA sequence
    • >TC010912 RA
      tggcggtca tcaaataaa cattgtaaa caatataat 
      tgttttatc tctcattat ttcaccaga gatgcaaca 
      aaattgagt gtggtggtc cgaaaaatg aatccgaaa 
      tcactgttt ttgctagtt tttgtgact attttgggt 
      gtaacatcc agtgaagtg tcattcaaa aatgttacc 
      ctttggacg tgggagaaa aatatcaac accatcgat 
      tccgcaaac aatctcggg acttattta cctaaaaag 
      tggacttcg acaattatt gtaatcgga acaatacca 
      attttacat gaaaattcc gtcacaaac gttgaaaat 
      gtgaaggaa cttcaattc ctcaaaaat caaatcgtt 
      gaaatacgt ccaggtgcg ttcaaaaat ttaaacagt 
      gtgaaaaca ttgaaaatt aatggaagc aacatcaca 
      accatcgct ggagttttt gataatctc actttagag 
      cagctcaac ttaaataac aaccaaatc tcggtaatc 
      ccaaaagga gttgtgaaa aatcccaat ttgaaagga 
      ctgagcttt gccggtaat ttaatcgaa actattgaa 
      gaaagtgct tttgaccat cttccaaac ttggaaacg 
      ttggattta tttcaaaat ttactctca gatcttcca 
      ctcggaatt ttcaaacat ttaccccat ctcacgatt 
      gtttcgtta agcaataat aaattcacc acaatcaag 
      gaagaaatt ttcgacagc tcgtcaata cgttaccta 
      tatttggat ggtaattta ctgaaaacc gttccagaa 
      aatttcatg ggccgaatg ccaaattta cagggaatt 
      tacctaaat cgaaaccac ttaaaaaca attcctgct 
      ggcatcttc aataacaaa actttgctt cacatagag 
      ctagccaat aacgaaatt tcccacatt gcaccagat 
      gcattcaac aacatgccc aacctggca aatttaatt 
      ttgtcgagt aataaattg gcgcaatat aacaacaac 
      tggttgcgt aattgtccg aacatctat cgcctattt 
      ctttccaat aatctcatc gagcaactt ccagtccaa 
      gcatttaaa aatatttac aaccaaaaa gaacagtgg 
      gttaagtta aacagcaac aaaattaag aaaattaca 
      gctgaaaat ttcaaagga tggaaacta gtcgatacc 
      ttggatctg tctcagaat gaaatagaa caatggcat 
      gaggattat cttgcagat gctgaattt atccgggct 
      ctcaacctt gcatataac aagattacg tgtctggac 
      ggtgattta gataaaatt tttaaagcc aaaaaagcc 
      acttattta agtggcaat ccgttgagt gaagagtgt 
      aaaagcaaa attcgggaa tggtccgca agaaatcaa 
      gacaaagtc agtttgtga attccaaca ataaataaa 
      taaaatctt aagtattat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY