Status
Please wait ...
Gene information
General information
TC010910 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1481411..1486042
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010910
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01760

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010910 RA
      MKLICTLCL FLVYTWTSS AKQITNSRI IGGITAFAG 
      QFPFAVAIE TTTKDGKYF CGGTLLNDQ WIITAAQCA 
      DGALLFSIQ IGATSLSDP DENRLVLAT SEYVLHPEY 
      DPATLKNDI ALIELRIPI QFSNYILPI HGLPEAALE 
      AGVRVVALG WGQTSDEDA GLSDKLKFV TVTSLTNDE 
      CRLVYGNQI TDQMVCVEG NYNEGSCKG DTGSPLVRV 
      ISLGNALLI GVASFVSGN GCESTDPSG YTRISPYSL 
      NKMKYLFIL SVLVHSSQS ASLAKNGRI IGGQTAIVG 
      QFPHAAAIT VQTEDSRFF CGGTLIDKE WVLTAGQCV 
      DGAVLFTIQ LGSNSLHDA DSNRIIVAT SEFVLHPNF 
      DPMTLENDI GLIKLRMPV EFSDYLNKV NVLGAHYVV 
      ESTVLMALG WGQTSDSSS SLSDDLRWV NVVAISNDE 
      CRLTYDNQI TNVTLCVEG NFNEGSCYG DTGSGLIYA 
      VSGYHILLG IASFVSGNG CESTDPSGY IRTSDYRDW 
      IRNITNV
    CDS sequence
    • >TC010910 RA
      atgaaactc atttgcacc ttgtgcctg ttccttgtg 
      tatacttgg acatcatca gcgaaacaa atcacaaat 
      agcagaatc attggaggg atcacagct ttcgctggc 
      cagtttcct ttcgctgtg gccatcgaa accacgacc 
      aaagacggc aaatacttc tgtggtggc actttgctt 
      aatgaccag tggatcatc actgcagct cagtgtgcc 
      gatggggcc ctgcttttt tctatccaa attggagca 
      acaagttta agcgatcct gatgaaaat cgcctagtt 
      ttggccaca tctgagtac gttctacac ccagaatat 
      gacccagcg acacttaaa aacgacatt gccttaatc 
      gagctacgt attcccatc caattttcc aattatatt 
      cttccaatt catggtctc cctgaggct gcgttggag 
      gcaggtgtc cgagttgtt gctctgggt tggggacag 
      accagtgat gaggacgct ggtctttcc gataaactc 
      aaattcgtg acggtgacc agtctaaca aatgatgaa 
      tgtcgactg gtttacggt aatcaaata actgatcag 
      atggtttgt gttgaggga aattataac gaagggtct 
      tgcaaaggg gatactgga agccctctt gtgcgtgtt 
      atttcttta ggtaatgct cttctgatc ggagttgca 
      agttttgta agtgggaat ggatgcgaa agcactgat 
      ccttcagga tacaccaga atttcacca tatagtttg 
      aacaaaatg aaatatttg ttcatttta tcagttttg 
      gttcactct tcgcaaagt gccagttta gcaaaaaat 
      ggccgaatt attggtggc caaacagcg attgttggt 
      caatttccg cacgcagct gctatcaca gtacaaact 
      gaagacagt cgatttttt tgcggaggc acccttatt 
      gacaaggaa tgggttcta acagccggg caatgtgtt 
      gatggcgct gtcctcttt acaattcaa ttggggagt 
      aacagcttg catgacgct gactcaaac cgtatcatt 
      gtggccacg tccgaattt gtattacat cctaatttt 
      gatccaatg actttggaa aatgatatt ggacttata 
      aagcttcga atgccagtc gagtttagc gattatttg 
      aacaaagtg aacgttttg ggggctcat tatgttgta 
      gaaagtaca gttcttatg gctttgggt tggggtcaa 
      actagtgat agtagttct agtcttagt gatgatctt 
      agatgggtt aatgtcgta gctatatca aatgatgag 
      tgtcgatta acttatgat aatcagata acaaacgtt 
      actctttgc gttgagggt aactttaat gaaggatct 
      tgttatggc gatacagga agcggctta atttatgct 
      gtcagtggg taccatata ctcctcgga atagcaagt 
      tttgttagt ggcaatggt tgcgaaagc actgatcct 
      tctggatat attagaacg agtgattac cgagactgg 
      atcaggaat attacaaat gtttaa
    mRNA sequence
    • >TC010910 RA
      ttcaaaaac aaatcaaga tgaaactca tttgcacct 
      tgtgcctgt tccttgtgt atacttgga catcatcag 
      cgaaacaaa tcacaaata gcagaatca ttggaggga 
      tcacagctt tcgctggcc agtttcctt tcgctgtgg 
      ccatcgaaa ccacgacca aagacggca aatacttct 
      gtggtggca ctttgctta atgaccagt ggatcatca 
      ctgcagctc agtgtgccg atggggccc tgctttttt 
      ctatccaaa ttggagcaa caagtttaa gcgatcctg 
      atgaaaatc gcctagttt tggccacat ctgagtacg 
      ttctacacc cagaatatg acccagcga cacttaaaa 
      acgacattg ccttaatcg agctacgta ttcccatcc 
      aattttcca attatattc ttccaattc atggtctcc 
      ctgaggctg cgttggagg caggtgtcc gagttgttg 
      ctctgggtt ggggacaga ccagtgatg aggacgctg 
      gtctttccg ataaactca aattcgtga cggtgacca 
      gtctaacaa atgatgaat gtcgactgg tttacggta 
      atcaaataa ctgatcaga tggtttgtg ttgagggaa 
      attataacg aagggtctt gcaaagggg atactggaa 
      gccctcttg tgcgtgtta tttctttag gtaatgctc 
      ttctgatcg gagttgcaa gttttgtaa gtgggaatg 
      gatgcgaaa gcactgatc cttcaggat acaccagaa 
      tttcaccat atagtttga acaaaatga aatatttgt 
      tcattttat cagttttgg ttcactctt cgcaaagtg 
      ccagtttag caaaaaatg gccgaatta ttggtggcc 
      aaacagcga ttgttggtc aatttccgc acgcagctg 
      ctatcacag tacaaactg aagacagtc gattttttt 
      gcggaggca cccttattg acaaggaat gggttctaa 
      cagccgggc aatgtgttg atggcgctg tcctcttta 
      caattcaat tggggagta acagcttgc atgacgctg 
      actcaaacc gtatcattg tggccacgt ccgaatttg 
      tattacatc ctaattttg atccaatga ctttggaaa 
      atgatattg gacttataa agcttcgaa tgccagtcg 
      agtttagcg attatttga acaaagtga acgttttgg 
      gggctcatt atgttgtag aaagtacag ttcttatgg 
      ctttgggtt ggggtcaaa ctagtgata gtagttcta 
      gtcttagtg atgatctta gatgggtta atgtcgtag 
      ctatatcaa atgatgagt gtcgattaa cttatgata 
      atcagataa caaacgtta ctctttgcg ttgagggta 
      actttaatg aaggatctt gttatggcg atacaggaa 
      gcggcttaa tttatgctg tcagtgggt accatatac 
      tcctcggaa tagcaagtt ttgttagtg gcaatggtt 
      gcgaaagca ctgatcctt ctggatata ttagaacga 
      gtgattacc gagactgga tcaggaata ttacaaatg 
      tttaactat aaaattctc aaaaataaa cacatttta 
      caaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY