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Gene information
General information
TC010901 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1610544..1626769
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010901
    iBeetle screen iB_01758

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010901 RA
      MTRILLPFI LIAFVRGET SVYILSENL EQVVTSTTL 
      QWVHSSSIN ETTLGNAVI AALQVVKDP DNPEEPIGS 
      KPVYVCRAR TNYVWVSGQ LRPKSKVCI VSMYKKVSE 
      HPNFEVLIN VENSARLSW VYKDKYVTA NLAGGVTSG 
      EGREGPLER TFIARHETN NHNKLGSLN YHVGKFNPG 
      ENLGVFHVV DQNNNEITH EMGQILVET EPIRYEFKS 
      VKFDRIRAK HHKKKMVLG HATLKNTER GVQRVDTVI 
      SYNYTYSIY WGKAHGLLT GLNFTVSLP DGVKTGTWA 
      LPKSENVVD TKTIEKYLD EGTAVNVTL WGNYTDSDV 
      PYTATVYAV YKDKEQLPR PIFDNKRER LITDVSIEF 
      GPVYFLHNN SHVPTTTTT TTTTTTTTS ATTSRTKST 
      THKVVPLEI PSVVQVGNS EKHPDEASA IKIHENSSM 
      MSDASNAES EIATSTTKK NTLKASSST RVLADLVLL 
      GAVSLIVRR VT
    CDS sequence
    • >TC010901 RA
      atgacgagg atattactt ccgtttatc ctcatcgct 
      ttcgtccgc ggagaaacc tcggtctac atcctctcc 
      gaaaattta gaacaagtg gtaacttcg acaaccctc 
      cagtgggtg cactcgtcc agtatcaac gaaaccacc 
      cttggcaat gccgtcatc gcagccttg caagtagtc 
      aaggatccc gacaatccc gaagaaccc atcggcagc 
      aaaccggtc tacgtttgc cgcgccaga accaactac 
      gtgtgggtc tcaggccag ttgaggccc aaatccaaa 
      gtatgcatc gtatccatg tacaagaaa gtctccgaa 
      cacccaaat ttcgaagtg ttgataaac gtcgaaaac 
      agcgctcgc ctcagctgg gtgtacaaa gacaaatac 
      gtaactgca aacctcgcc ggaggtgtt acaagtggc 
      gagggtcgt gaaggaccc ctcgaaagg actttcatc 
      gccagacac gaaaccaac aaccataac aaactcggt 
      tctttgaac tatcacgtt gggaaattt aatccgggc 
      gaaaacctg ggtgttttc cacgtcgta gatcagaac 
      aacaacgag atcacacac gaaatgggg cagatttta 
      gtcgagacg gaacccatc agatacgaa ttcaagtcc 
      gtcaagttc gacaggatt cgtgccaaa caccacaag 
      aaaaaaatg gttttgggg cacgcaact ttgaagaac 
      actgaacgc ggcgtgcag cgagttgac accgtcatc 
      agttacaac tacacctac tccatttat tggggcaaa 
      gcgcacgga cttttaacc ggcctcaac ttcaccgtg 
      tctctccca gacggcgtg aaaacaggc acctgggcc 
      ctgcctaag agcgaaaac gtggtcgac accaaaacc 
      atcgagaag tacctggat gaaggcacc gctgtcaac 
      gtcacactc tggggcaac tacaccgac tcggacgtg 
      ccctacact gccaccgtt tacgcagtg tacaaagat 
      aaggaacaa ttacctcgg ccaatattt gacaacaaa 
      cgcgaacgc ctgataacg gatgtgtcc atcgagttc 
      ggtcctgtg tacttcttg cacaacaac agtcacgtc 
      cctaccacc accaccacg accactacg acaacaacc 
      acgacgtca gcgaccacc agtaggaca aaatcaacg 
      actcataag gtcgttccg ttggagatt ccctcagtt 
      gtgcaagtt ggaaattcg gagaaacac cccgatgaa 
      gcctcggcg atcaaaatc cacgagaac agcagcatg 
      atgtcggac gccagcaac gccgagtcc gaaattgcg 
      acgtcaacg acgaagaaa aacacgctg aaagccagc 
      tcttcaacg cgagtcctc gccgatttg gtgctgttg 
      ggggcggtg tcgctgatt gtgcgtcgg gtgacgtaa
    mRNA sequence
    • >TC010901 RA
      aatctcgtt aagagagtg cgtgaagta gcgaaaatc 
      gttgagtgt cgtgaccag tgcgaaaat aaataatac 
      agggtgacc catatctat tttcttgtg gatttttac 
      ccaaacgcc atatcagga tttgcgatt taagcgaga 
      agatgacga ggatattac ttccgttta tcctcatcg 
      ctttcgtcc gcggagaaa cctcggtct acatcctct 
      ccgaaaatt tagaacaag tggtaactt cgacaaccc 
      tccagtggg tgcactcgt ccagtatca acgaaacca 
      cccttggca atgccgtca tcgcagcct tgcaagtag 
      tcaaggatc ccgacaatc ccgaagaac ccatcggca 
      gcaaaccgg tctacgttt gccgcgcca gaaccaact 
      acgtgtggg tctcaggcc agttgaggc ccaaatcca 
      aagtatgca tcgtatcca tgtacaaga aagtctccg 
      aacacccaa atttcgaag tgttgataa acgtcgaaa 
      acagcgctc gcctcagct gggtgtaca aagacaaat 
      acgtaactg caaacctcg ccggaggtg ttacaagtg 
      gcgagggtc gtgaaggac ccctcgaaa ggactttca 
      tcgccagac acgaaacca acaaccata acaaactcg 
      gttctttga actatcacg ttgggaaat ttaatccgg 
      gcgaaaacc tgggtgttt tccacgtcg tagatcaga 
      acaacaacg agatcacac acgaaatgg ggcagattt 
      tagtcgaga cggaaccca tcagatacg aattcaagt 
      ccgtcaagt tcgacagga ttcgtgcca aacaccaca 
      agaaaaaaa tggttttgg ggcacgcaa ctttgaaga 
      acactgaac gcggcgtgc agcgagttg acaccgtca 
      tcagttaca actacacct actccattt attggggca 
      aagcgcacg gacttttaa ccggcctca acttcaccg 
      tgtctctcc cagacggcg tgaaaacag gcacctggg 
      ccctgccta agagcgaaa acgtggtcg acaccaaaa 
      ccatcgaga agtacctgg atgaaggca ccgctgtca 
      acgtcacac tctggggca actacaccg actcggacg 
      tgccctaca ctgccaccg tttacgcag tgtacaaag 
      ataaggaac aattacctc ggccaatat ttgacaaca 
      aacgcgaac gcctgataa cggatgtgt ccatcgagt 
      tcggtcctg tgtacttct tgcacaaca acagtcacg 
      tccctacca ccaccacca cgaccacta cgacaacaa 
      ccacgacgt cagcgacca ccagtagga caaaatcaa 
      cgactcata aggtcgttc cgttggaga ttccctcag 
      ttgtgcaag ttggaaatt cggagaaac accccgatg 
      aagcctcgg cgatcaaaa tccacgaga acagcagca 
      tgatgtcgg acgccagca acgccgagt ccgaaattg 
      cgacgtcaa cgacgaaga aaaacacgc tgaaagcca 
      gctcttcaa cgcgagtcc tcgccgatt tggtgctgt 
      tgggggcgg tgtcgctga ttgtgcgtc gggtgacgt 
      aaagtggtc taatcttcg aacttggac ttgttttga 
      tcgagcgcg gcgcgactg tgagtgcaa agcgaagga 
      agaatgcag acggtgtga atcgggctt taattattc 
      tatcattat gtatcggtt atgtttcat cagtagtca 
      tcattgaag agtgtatat agactgtaa atattgtgg 
      acaaggata aggtgtgtc ataatagtg atggtgata 
      ttttgttaa ttgcgacaa gtggcgtcc atgtttgta 
      gcgagtaag atggcaagg atatatttt tgctattat 
      caaattcag gctagtttg cagtattga cgaggagga 
      tgtatgtac aaagtaaga cgtaaacgc gacttcatt 
      ccattgttt cagtacaaa actttttcc aaacggtag 
      aatagaacg gacgaaagc tttcaactg attcatttg 
      acgtattta acatagata ttcaggtac atgtgataa 
      tgttgtatt tatgctagg cttcttctc aagctttcc 
      aagtattca aatcaaaga caaaaaatc aaaaatcac 
      taaaaatgt tgaaaaatt tccaaatgt tcgtgttcc 
      taactagta agtcaacaa gcacttgat gttgcctag 
      atctagaag accggtgat tttgattga atactacag 
      acagcgtga ttatccatt ggactgaca catgtaaaa 
      taaatactt gttctaaat gtacatttc atgtaattg 
      tagatattt taaaaatat acttaaaac cgtata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY