Status
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Gene information
General information
TC010894 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1889168..1890815
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010894
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01757

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    antenna shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs number increased - Penetrance (percentage of animals/eggs on slide): more than 80%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010894 RA
      MFTKYFLCV CIVCYFSLA QETKPLQYN DRNFDTKMN 
      EHEVALVLF YAPWCNHCI QFLPKFADA AKQSEESSR 
      PIAFVMVDC ENDGKQTCE KFGVSSFPT LKIFRNGKF 
      LKAYEGPRE APAIAKYMK AQVDGDSRE LGSVAELED 
      FLSTDEVSV VGFFESDSY LKVVFFKVV DKMKHKIRF 
      GHSTSEAVM LQQEVADGI VLFRPPHLH NKFEKSSVL 
      YEGDAETDE IIQFIMDNY HGLVGHRRK DLEEFEKEP 
      LVAVFYAVD YKKNTKRTN YWRNRVLPV AKKYENQFY 
      FVISDKDEF KSELKGHKL YEKNLTKPV VLAQNFRKR 
      LFKMKEPFS TEALDNFVQ DILDGKLEP EMKSEPIPE 
      VNNGSVTIA VAKNFDKLV YDNGVDTVV LLTGPDCDE 
      CIIVEKPYE ELGEKMKNE DIAVIKMDI SKNSSPQEL 
      ELDGIPSIF FLPKDSKDY PILHKGDFY IDDFIQFVA 
      QHATNELKG YYRNGKPRP ERTEL
    CDS sequence
    • >TC010894 RA
      atgtttaca aaatacttc ctctgcgtc tgcatcgta 
      tgttacttc agtctggcc caagaaaca aagcctttg 
      caatacaac gacaggaac tttgacacc aaaatgaac 
      gagcacgaa gtggccctt gtgttgttt tacgcacct 
      tggtgtaac cactgcatc cagtttctg cccaaattt 
      gcggacgct gcaaagcaa tcagaggag agcagccgt 
      ccgattgcc tttgtcatg gttgactgt gaaaacgac 
      ggtaaacaa acttgtgaa aagtttggc gtcagctcg 
      ttccccaca ctgaaaatt ttccgcaac ggcaagttc 
      ttgaaagcg tacgagggg cccagagag gccccagct 
      atagcaaag tatatgaag gcgcaagtt gacggcgac 
      tcgcgggag ctgggcagt gttgcagag ctggaggat 
      tttttatct actgacgag gtctcggtg gttggtttt 
      tttgaaagt gactcttat ttgaaagtg gttttcttt 
      aaagtggtt gataaaatg aaacacaag ataaggttc 
      gggcactcc acctcggag gctgtgatg ctgcagcag 
      gaggttgcc gacgggatt gtgctgttc cgtcctcca 
      catctacac aacaagttc gaaaagagc agtgttttg 
      tatgaagga gacgccgaa actgacgaa atcattcag 
      tttataatg gacaactac cacggtttg gtgggacac 
      cgcagaaag gacttggag gagtttgaa aaagagccg 
      cttgtggcc gttttctac gccgttgat tacaagaag 
      aatactaag aggacaaat tactggcga aacagagtc 
      ctgccagtt gcgaagaaa tacgaaaac cagttctat 
      ttcgtcatc tctgataag gacgagttc aaatcagaa 
      cttaaaggg cataagctt tacgaaaaa aacctgaca 
      aagcctgtg gttttggcc caaaacttc agaaaacgc 
      ttgttcaaa atgaaagaa ccattctct actgaggct 
      cttgataac ttcgtgcag gatatcctc gacggaaaa 
      ctggagcct gaaatgaaa agtgaaccg atccctgaa 
      gtcaacaat ggatcggtt acaattgct gtggcgaaa 
      aactttgat aaacttgtt tacgataat ggagttgac 
      acagttgtt ctactgact ggccccgat tgcgatgaa 
      tgcataatt gtcgaaaag ccctatgaa gaactcggt 
      gaaaaaatg aagaacgaa gacattgca gttattaaa 
      atggatatt tcaaaaaat agctcgcct caagaatta 
      gagttagac ggtatccct tctatattt ttcctgccg 
      aaggattct aaagactat ccgattcta cacaaaggt 
      gatttttat atagacgat tttatacaa tttgttgct 
      caacatgca acaaacgaa cttaaagga tattacaga 
      aacgggaaa cctagaccg gaaagaact gaactttga
    mRNA sequence
    • >TC010894 RA
      tgatgacac taaacaagt ttcactgac agcattttg 
      tattattta ttttgacac gaatagttt gtttaaaaa 
      aatattgaa acatgttta caaaatact tcctctgcg 
      tctgcatcg tatgttact tcagtctgg cccaagaaa 
      caaagcctt tgcaataca acgacagga actttgaca 
      ccaaaatga acgagcacg aagtggccc ttgtgttgt 
      tttacgcac cttggtgta accactgca tccagtttc 
      tgcccaaat ttgcggacg ctgcaaagc aatcagagg 
      agagcagcc gtccgattg cctttgtca tggttgact 
      gtgaaaacg acggtaaac aaacttgtg aaaagtttg 
      gcgtcagct cgttcccca cactgaaaa ttttccgca 
      acggcaagt tcttgaaag cgtacgagg ggcccagag 
      aggccccag ctatagcaa agtatatga aggcgcaag 
      ttgacggcg actcgcggg agctgggca gtgttgcag 
      agctggagg attttttat ctactgacg aggtctcgg 
      tggttggtt tttttgaaa gtgactctt atttgaaag 
      tggttttct ttaaagtgg ttgataaaa tgaaacaca 
      agataaggt tcgggcact ccacctcgg aggctgtga 
      tgctgcagc aggaggttg ccgacggga ttgtgctgt 
      tccgtcctc cacatctac acaacaagt tcgaaaaga 
      gcagtgttt tgtatgaag gagacgccg aaactgacg 
      aaatcattc agtttataa tggacaact accacggtt 
      tggtgggac accgcagaa aggacttgg aggagtttg 
      aaaaagagc cgcttgtgg ccgttttct acgccgttg 
      attacaaga agaatacta agaggacaa attactggc 
      gaaacagag tcctgccag ttgcgaaga aatacgaaa 
      accagttct atttcgtca tctctgata aggacgagt 
      tcaaatcag aacttaaag ggcataagc tttacgaaa 
      aaaacctga caaagcctg tggttttgg cccaaaact 
      tcagaaaac gcttgttca aaatgaaag aaccattct 
      ctactgagg ctcttgata acttcgtgc aggatatcc 
      tcgacggaa aactggagc ctgaaatga aaagtgaac 
      cgatccctg aagtcaaca atggatcgg ttacaattg 
      ctgtggcga aaaactttg ataaacttg tttacgata 
      atggagttg acacagttg ttctactga ctggccccg 
      attgcgatg aatgcataa ttgtcgaaa agccctatg 
      aagaactcg gtgaaaaaa tgaagaacg aagacattg 
      cagttatta aaatggata tttcaaaaa atagctcgc 
      ctcaagaat tagagttag acggtatcc cttctatat 
      ttttcctgc cgaaggatt ctaaagact atccgattc 
      tacacaaag gtgattttt atatagacg attttatac 
      aatttgttg ctcaacatg caacaaacg aacttaaag 
      gatattaca gaaacggga aacctagac cggaaagaa 
      ctgaacttt gaaggacga acgcacaaa acgctaaat 
      tgtcatgtt gcagtatgt ttttttgta attaaaatg 
      tccagcaca atacataat attacacaa a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY