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Gene information
General information
TC010885 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4968170..4969704
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010885
    iBeetle screen iB_01756

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010885 RA
      MDGSIKELE VASSSCNGE EGGRGCRNK RKLSEPICC 
      PVCSVTLRI SEVQSHFMV EVDKLGKIP TNKHKSNGR 
      SVPSSSNTS ESNADKTWE TFQKVRLNR QNRHRSKTK 
      KRKTEEVLC PICNKETSE EITLHVEKC LRRTERNES 
      ESDESIDVE AFEEYEWAG QSRVRITSL LPGGVINNL 
      GTSISMTEE DEDLNVDGD DAQTFGSPQ YSERDVILL 
      PGDPENVAL RKAVTGEDP KRMQPEENS DSVNTQPKG 
      DPLVEVLKN RIRELESKE KNKEEVYKC LICMERYRT 
      PVISVCCWH VHCEQCWLQ TLGAKKLCP QCNMITSPT 
      DLRKIYM
    CDS sequence
    • >TC010885 RA
      atggatgga tccataaaa gaactagaa gtagcaagt 
      agcagttgt aatggtgaa gaaggtggt cgcggctgt 
      cgcaacaag agaaaatta tcagaaccg atctgctgc 
      cccgtctgt agcgtaact ctgcgtatt tccgaagtc 
      caatcccac tttatggta gaagtagac aagctaggt 
      aaaatcccc acaaacaag cacaaatcg aacgggcgc 
      agcgtcccc agctcatcc aacaccagt gaaagcaat 
      gccgacaag acgtgggaa accttccag aaggtccga 
      ctcaaccgc cagaaccgg caccggtcc aaaacaaaa 
      aaacgcaag actgaggag gtcctgtgc ccgatttgc 
      aacaaagaa acctcggag gaaataacc ctgcatgtg 
      gaaaagtgt ttgcgccgt actgaacga aacgagagt 
      gaaagtgac gagagtatc gatgtggag gcgttcgag 
      gagtacgag tgggctgga cagtcgcgg gtccgcata 
      acgtccctg ctccccggg ggtgtgatt aacaattta 
      gggacttcg ataagtatg actgaagag gacgaagac 
      ttaaacgtc gacggcgac gacgcccag acgttcggc 
      tcgccgcaa tattccgaa cgcgatgtt attctccta 
      cccggcgat cccgaaaat gtcgcgctg cggaaagcc 
      gtcaccggg gaggacccc aagaggatg caacctgaa 
      gagaacagc gactcggtc aacacgcag cctaaaggc 
      gacccgctg gtcgaggtc ctgaaaaac aggatccgg 
      gagctggaa agcaaggaa aagaacaag gaggaggtt 
      tacaaatgt ctcatttgt atggagaga taccggacg 
      ccggtcatc tccgtgtgt tgttggcac gtgcattgt 
      gaacagtgc tggttacag acgctcgga gcgaaaaaa 
      ctctgcccc cagtgcaac atgatcacg tcacccaca 
      gatctgagg aaaatctac atgtaa
    mRNA sequence
    • >TC010885 RA
      ggccttacg caaaatcat aaattttta cataacacc 
      attaaaaat gcgcattaa attcaaatt ctagtgaat 
      tgcgtcatg gcaattttg gcagttggc agccatgcc 
      tttcaaact tgtttattg actgtgtac ttcttttcc 
      ccgaatatt tattgtgtg aaaatcaaa tttagtttt 
      aacaatcct actttaaat tgtgcaaaa ttaattaat 
      ttacgccaa gcttttgaa gcgtccaat tgaaatgac 
      catattttt gacgtacaa atatgtgta aacaaggaa 
      actaaccta agatgcaac taataaaca aaatcttcc 
      aacatctaa acaaaaaca gtttgaaat ttaaataat 
      ttgttaaaa aatcggtgt acgtgtctc gtcgcgcgt 
      atcgtaagt gttatttga tgggattat ggatggatc 
      cataaaaga actagaagt agcaagtag cagttgtaa 
      tggtgaaga aggtggtcg cggctgtcg caacaagag 
      aaaattatc agaaccgat ctgctgccc cgtctgtag 
      cgtaactct gcgtatttc cgaagtcca atcccactt 
      tatggtaga agtagacaa gctaggtaa aatccccac 
      aaacaagca caaatcgaa cgggcgcag cgtccccag 
      ctcatccaa caccagtga aagcaatgc cgacaagac 
      gtgggaaac cttccagaa ggtccgact caaccgcca 
      gaaccggca ccggtccaa aacaaaaaa acgcaagac 
      tgaggaggt cctgtgccc gatttgcaa caaagaaac 
      ctcggagga aataaccct gcatgtgga aaagtgttt 
      gcgccgtac tgaacgaaa cgagagtga aagtgacga 
      gagtatcga tgtggaggc gttcgagga gtacgagtg 
      ggctggaca gtcgcgggt ccgcataac gtccctgct 
      ccccggggg tgtgattaa caatttagg gacttcgat 
      aagtatgac tgaagagga cgaagactt aaacgtcga 
      cggcgacga cgcccagac gttcggctc gccgcaata 
      ttccgaacg cgatgttat tctcctacc cggcgatcc 
      cgaaaatgt cgcgctgcg gaaagccgt caccgggga 
      ggaccccaa gaggatgca acctgaaga gaacagcga 
      ctcggtcaa cacgcagcc taaaggcga cccgctggt 
      cgaggtcct gaaaaacag gatccggga gctggaaag 
      caaggaaaa gaacaagga ggaggttta caaatgtct 
      catttgtat ggagagata ccggacgcc ggtcatctc 
      cgtgtgttg ttggcacgt gcattgtga acagtgctg 
      gttacagac gctcggagc gaaaaaact ctgccccca 
      gtgcaacat gatcacgtc acccacaga tctgaggaa 
      aatctacat gtaacctta ggaactttt aatttatat 
      ttttaataa actggccca gaactttat ttatacacg 
      tgtgatata tgcagacag aatttatta taattttac 
      tagctagtt tttgtttca tttgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY