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Gene information
General information
TC010879 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 4746641..4748810
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010879
    iBeetle screen iB_01755
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010879 RA
      MGLFSGKCV SKARLDGKT AVVTGANTG IGKETVKDF 
      FQRGARVIV ACRNLDKAN QAVEDIKKE FSDGENLGE 
      LMVTQLDLT SLKSVRNCA KVILETEKR IDLLINNAG 
      VMMCPEGRT EDGFEMQFG TNHLGHFLL TLLLLPKIC 
      QSTPARIVN VSSVAHKYG CIDFEDLNW QKRKYSSLG 
      AYQQSKLAN ILFTKELVR RLAEANVTG VNVYSLHPG 
      VIRTELGRH LDYRLRWLW RIFSFLIKT PDQGAQTTI 
      YCAVDEKCA NETGLYYAD CAVAAVAPA AQNSVDAKR 
      LWDESLKLV GLEPNYNPF TTV
    CDS sequence
    • >TC010879 RA
      atggggcta ttcagtggc aagtgtgtc tcaaaggcg 
      cgtctggac ggcaaaact gcagtcgtt acgggcgcc 
      aatacgggc attggaaaa gaaacggtg aaggatttt 
      tttcaacga ggtgcccgt gttattgtg gcttgtcga 
      aatctggac aaggcgaat caggccgtt gaggacata 
      aaaaaagaa ttctcagat ggtgagaat ttgggcgag 
      ttaatggta acacagttg gatttaact agtctaaag 
      tcggtgcga aattgcgcg aaagttatt ttggaaacg 
      gagaaacga attgatctg ttgataaat aatgcgggc 
      gttatgatg tgtccggag ggccggact gaggatggt 
      ttcgagatg cagttcggt acaaatcat ttaggacac 
      tttcttctg actttgttg ttactgccc aaaatctgc 
      caaagtaca cctgcccga atcgtgaat gtgtcttcc 
      gtggctcac aaatacggt tgtatcgac ttcgaggac 
      ttgaattgg cagaaacgc aaatacagc tcactggga 
      gcgtaccag caaagcaaa ttagccaat attttattc 
      accaaagag ctagttcga agacttgca gaggcaaat 
      gtaacagga gtcaatgtg tattccctc caccctgga 
      gtcattcga actgagttg gggcgtcat ttggattac 
      aggcttcgt tggctttgg cgtattttc tcctttttg 
      attaaaacc cccgaccag ggggctcaa acaacaatt 
      tactgcgct gttgatgaa aagtgtgcc aatgagact 
      ggcctttat tacgccgat tgtgcagtg gcggccgtc 
      gctcccgct gcacaaaat tcagtcgac gctaagcgt 
      ctgtgggac gaaagcttg aaacttgta ggacttgag 
      ccgaactat aatccattt acaaccgtc taa
    mRNA sequence
    • >TC010879 RA
      ttaggctgg catttgtcg catagttgc gccaaatgg 
      ggctattca gtggcaagt gtgtctcaa aggcgcgtc 
      tggacggca aaactgcag tcgttacgg gcgccaata 
      cgggcattg gaaaagaaa cggtgaagg atttttttc 
      aacgaggtg cccgtgtta ttgtggctt gtcgaaatc 
      tggacaagg cgaatcagg ccgttgagg acataaaaa 
      aagaattct cagatggtg agaatttgg gcgagttaa 
      tggtaacac agttggatt taactagtc taaagtcgg 
      tgcgaaatt gcgcgaaag ttattttgg aaacggaga 
      aacgaattg atctgttga taaataatg cgggcgtta 
      tgatgtgtc cggagggcc ggactgagg atggtttcg 
      agatgcagt tcggtacaa atcatttag gacactttc 
      ttctgactt tgttgttac tgcccaaaa tctgccaaa 
      gtacacctg cccgaatcg tgaatgtgt cttccgtgg 
      ctcacaaat acggttgta tcgacttcg aggacttga 
      attggcaga aacgcaaat acagctcac tgggagcgt 
      accagcaaa gcaaattag ccaatattt tattcacca 
      aagagctag ttcgaagac ttgcagagg caaatgtaa 
      caggagtca atgtgtatt ccctccacc ctggagtca 
      ttcgaactg agttggggc gtcatttgg attacaggc 
      ttcgttggc tttggcgta ttttctcct ttttgatta 
      aaacccccg accaggggg ctcaaacaa caatttact 
      gcgctgttg atgaaaagt gtgccaatg agactggcc 
      tttattacg ccgattgtg cagtggcgg ccgtcgctc 
      ccgctgcac aaaattcag tcgacgcta agcgtctgt 
      gggacgaaa gcttgaaac ttgtaggac ttgagccga 
      actataatc catttacaa ccgtctaag acttacgaa 
      gatattctt ttagttatt aaatttgat taaatgata 
      ggctattat gtaaaacac aggtgatat cttgtacgt 
      tcatgacac tcgagtaaa gttacaaaa taatcaata 
      tttttcgtt tgcgttatt ggggactag aaggttttt 
      cgcgtccct aattccatc agaaggaaa tgagccggt 
      aatgatctt aaccttcag atgataaaa attaatatt 
      tacggtact tttgaactt gattattca attaatctt 
      cgtccgtga ccataagtg gctactcat ttgattatt 
      tacgtagaa aaatgcaca tgtagatac agtggaaaa 
      aatcgatta gaaatcaac tggagatat caggcgaca 
      ataaaaaat aatttggct aattacaac tatccctat 
      gcgtaaatt tggcactta aacacgagc catcacagt 
      ttttatgaa aatacaata actacttac aagttttaa 
      gcatgacct gccctaaat cttcaaaga aataaatat 
      ccctttgtt tttttggtg taattattt attccacag 
      taagttttt agggaaaca ataattatt ttacttact 
      tatttttta cccatttaa cctgatata ctctttgaa 
      aatgataaa tcccctcat gagactgaa aaaaaaatc 
      cgcatacga attcataaa acgttgcca ctaaacaca 
      tttttctaa tacctgtac tgtttaggt caaaataaa 
      aaagttgac aagttttag aatgcaaat gtttttttt 
      acttacgtt aataacata ataactggt gaggtaaga 
      gggcctgaa tgagagtcg gtttattta gacatttta 
      gattttttc cagtgttgt tgtaagctg gagttgcag 
      tccttggtt gtaagagaa tccagtttg acggggtag 
      cgggaacca ttccggttt taatccttt tagttccta 
      catctggta acagtgcta acccatgta ttattttat 
      ctatctatt aaatttgta tacatatga ggtgttgac 
      ctgactatt gatgaataa ataaaaaag tgatgaaat 
      a