| General information | |||
| TC010870 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 5073609..5080204 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010870 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01754 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
embryo/egg not developed/not fertilized -
Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
Days post injection
Fertility
egg production: number <50% of expected -
| Comment: No phenotype present! |
- >TC010870 RA
MASVSYQIA NLLEKMQSS DKDFRFMAT NDLMAELQK DSIKLDDDS ERKVVRMLL KLLEDKNGE VQNLAVKCL GPLVNKVKE FQVETIVEA LCTNMVSDK EQLRDISSI GLKTVIAEL PQAPGGLSG NICKRITGR LTTAIERQD DVSVQLEAL DIVTDLLYR FGNVLQSYH ASILNALLP QLCSQRQAV RKRTIAACS NLVLSCNNA LYDKLIEHL YNGMRTDNK NSQIRTYVQ CIAAVCRQS GHKFGEHIE KFVPLIQMW SAVEDDETR EFCLQAFES FINRCPKEI TPKISAITD LCLNYMTHD PNYNYDEDG DDRAMGAYD DEEEEENDE YSDDDDMSW KVRRAAAKC LESIICTRH ELLSEFYKV LSPVLISRF KEREENVKS DIFRAYIAL LRQTKSTVN VNIDPNSME QEEMPIYLL QQQVPMIVK GVQSQMKEK STKTRQDCF QLLKELCNV LPGALTHHI GDLIPGILF SLSEKNGSS NMKIDGLSF LLCLLASHP PEVFHPHIN ILLPHIIAN VGDNFYKIT AEALNVLKE LVKVIRPLD RDSNFDFTP FVKEIYLCT LSRLKTSDL DQEVKEKAI STMGQIICN LGDHLGSEL SCCLPLFLD RLRNEITRL TTVKALTKI AGSPLGIEL PILDEAVPV LGLFLRKNQ RALKLSTLQ LIDCLIKNY HVYMYVELL QHVIVEVPP LLDESDLHI AQYTLVILK SIAEYHPLA LQDLPTNIF PQILVLVKS PLLQGSALN SMLEFFKTL VQCNLPGLS YNDLLQFLV HPISISQGS GAGDAVIHK QAYYSLAKC VAAITVTMQ HQALQIVPQ FINEIRSSR HDAQKIFTL LVVGEIGRE IDLTGFANL EQIISEAFF AVSEEVKSA ASYALGSIA IGNLQQYLP FILTEDENE PRRQYLLLH SLKEVIACL SSTPEGIQQ LLPFVPAIW DTLYRHCES EEEGTRNVV SECLGKLTL IDPNHLLPK LKESLGSES SLMRTTIVT AIKFTISDQ PASIDALLR QCIGEFLNT LEDPDLNVR RVALVAFNS AAHNKPSLI RDLLDTILP RLYHETNIK RELIREVEM GPFKHTVDD GLDLRKAAF ECMYTLLDS CLDRIDIFE FINHVELGL KDHYDIKML TYLMVSRLA QICPGAVLQ SLDRLVEPL KATCTMKVK ANSVKQEYE KQDELKRSA LRAVVSLLS IPDADKNQH LNEFVTQIK TSPDLAQIF DSIQKDSAN NINDFFLMD QS
- >TC010870 RB
MAEEQQQGD ESNPYEQGD HAFELDDLE RALVTCELR SYVDQVVED AAEMASVSY QIANLLEKM QSSDKDFRF MATNDLMAE LQKDSIKLD DDSERKVVR MLLKLLEDK NGEVQNLAV KCLGPLVNK VKEFQVETI VEALCTNMV SDKEQLRDI SSIGLKTVI AELPQAPGG LSGNICKRI TGRLTTAIE RQDDVSVQL EALDIVTDL LYRFGNVLQ SYHASILNA LLPQLCSQR QAVRKRTIA ACSNLVLSC NNALYDKLI EHLYNGMRT DNKNSQIRT YVQCIAAVC RQSGHKFGE HIEKFVPLI QMWSAVEDD ETREFCLQA FESFINRCP KEITPKISA ITDLCLNYM THDPNYNYD EDGDDRAMG AYDDEEEEE NDEYSDDDD MSWKVRRAA AKCLESIIC TRHELLSEF YKVLSPVLI SRFKEREEN VKSDIFRAY IALLRQTKS TVNVNIDPN SMEQEEMPI YLLQQQVPM IVKGVQSQM KEKSTKTRQ DCFQLLKEL CNVLPGALT HHIGDLIPG ILFSLSEKN GSSNMKIDG LSFLLCLLA SHPPEVFHP HINILLPHI IANVGDNFY KITAEALNV LKELVKVIR PLDRDSNFD FTPFVKEIY LCTLSRLKT SDLDQEVKE KAISTMGQI ICNLGDHLG SELSCCLPL FLDRLRNEI TRLTTVKAL TKIAGSPLG IELPILDEA VPVLGLFLR KNQRALKLS TLQLIDCLI KNYHVYMYV ELLQHVIVE VPPLLDESD LHIAQYTLV ILKSIAEYH PLALQDLPT NIFPQILVL VKSPLLQGS ALNSMLEFF KTLVQCNLP GLSYNDLLQ FLVHPISIS QGSGAGDAV IHKQAYYSL AKCVAAITV TMQHQALQI VPQFINEIR SSRHDAQKI FTLLVVGEI GREIDLTGF ANLEQIISE AFFAVSEEV KSAASYALG SIAIGNLQQ YLPFILTED ENEPRRQYL LLHSLKEVI ACLSSTPEG IQQLLPFVP AIWDTLYRH CESEEEGTR NVVSECLGK LTLIDPNHL LPKLKESLG SESSLMRTT IVTAIKFTI SDQPASIDA LLRQCIGEF LNTLEDPDL NVRRVALVA FNSAAHNKP SLIRDLLDT ILPRLYHET NIKRELIRE VEMGPFKHT VDDGLDLRK AAFECMYTL LDSCLDRID IFEFINHVE LGLKDHYDI KMLTYLMVS RLAQICPGA VLQSLDRLV EPLKATCTM KVKANSVKQ EYEKQDELK RSALRAVVS LLSIPDADK NQHLNEFVT QIKTSPDLA QIFDSIQKD SANNINDFF LMDQS
- >TC010870 RA
atggctagt gtctcttac cagattgcc aatcttctt gagaagatg caatcatct gacaaagat tttcgattt atggccaca aacgattta atggccgaa ctgcagaaa gatagtatc aaattagat gatgattcg gaacgaaag gttgtaaga atgttgttg aaactactt gaagataaa aacggtgaa gtacaaaac ttggctgtc aaatgtctg gggccttta gttaacaaa gttaaggaa ttccaagtg gagaccatt gttgaggcc ttgtgcacc aacatggtc tccgacaag gagcaactg cgggatatt tccagcatt gggctcaag acggttatc gctgaactg ccacaggca cctgggggc ctctccgga aacatttgc aagcggatc accggtcgt ttgacgact gctatcgaa aggcaagac gacgtgtcc gtgcaactg gaagctctc gacattgtc acggatttg ttataccgc tttggcaac gttttgcaa tcctaccat gcctccata ttaaacgcc ctcttacct cagttatgc tcacaacgg caagcagtc cgcaaacgt acgattgca gcttgcagt aacttagtt ctctcgtgc aacaatgca ttgtacgat aaactaatc gagcatctg tacaacggg atgagaact gataacaag aactcccaa attcgcacc tacgtgcag tgcattgca gccgtgtgc cgacaatcg ggccacaag ttcggcgaa catattgaa aagttcgta cctttgatc caaatgtgg agcgctgtg gaagacgac gagaccaga gagttttgt ttacaagct tttgagagt tttatcaat cgttgtccc aaagaaatc accccgaaa atcagcgcc atcacagac ttgtgccta aattacatg actcatgac ccgaattac aactacgac gaagatggg gatgatcga gccatgggg gcttacgat gacgaggaa gaagaggag aatgacgag tacagcgat gacgatgac atgagctgg aaagtgaga cgtgcagct gccaagtgc ttggagtcg atcatctgc actcgccat gaattattg tctgaattt tacaaggtt ttgtcgcct gtgttaatc tcccggttt aaagagcgg gaagagaac gtgaaatca gatattttc cgtgcttac atcgcgctt ttgagacaa acaaaatcg actgttaac gtgaatatc gatccgaac tccatggag caggaggag atgcccatt tacttgctc cagcagcaa gttccaatg attgtgaaa ggcgtgcag tcgcaaatg aaggaaaag agcacgaag acgcgccaa gactgcttc cagctccta aaagaatta tgcaatgtc ctaccgggc gctttaacg caccacatt ggtgatctg attcccggt attctattt tcactgagt gaaaaaaat gggagcagt aacatgaaa attgacggc ttgtcgttt ctcctgtgt cttttggca tcgcaccct cctgaagtt tttcatccc catatcaat atcctccta ccgcacatc atagctaac gtgggtgat aacttttat aaaatcaca gctgaagct ttgaacgtt ctgaaagag ctggtcaag gtcattcgt cctctggac cgcgactcg aacttcgat ttcacgccc tttgtgaag gaaatctac ctctgtact ttatcgcgt ttaaagacg tccgattta gatcaagag gtcaaggaa aaagctatc tcgaccatg gggcaaatt atttgcaac ctgggtgat cacctaggg agtgaattg tcctgctgt cttcctttg ttcctcgat agattacgt aacgaaatc acgaggttg actacagtt aaagcgttg accaaaatt gcgggctct ccattgggc atcgaacta ccgattttg gacgaagca gtgccggtt ttggggctc ttcctgcgt aaaaaccag cgtgcttta aaactaagt actttgcaa ctcattgat tgtttaata aagaattac cacgtctac atgtacgtg gaattactc caacatgta atagtagaa gtgccacca ctccttgac gagtccgac ctgcacata gcccagtac actttggtg atcttgaag tcgattgcg gaataccac cccctggct ttgcaagac ctgccaaca aacatcttc ccgcaaatc ttggtcttg gtgaagtcg cctcttctg caaggttcc gccctgaat tccatgctg gaattcttc aaaactcta gtccaatgc aaccttccg ggattgtcc tacaacgac ttgttgcaa tttctcgtt cacccgatc agcattagc cagggttct ggggccggt gacgccgtc atccacaag caagcctat tactcgttg gccaaatgc gtggctgct ataacagtg acgatgcaa caccaagcg ttgcaaatc gtcccccaa ttcatcaac gaaattcgc tcttcgcgc cacgacgcc cagaagatc ttcactttg ctcgtggtc ggcgaaatc gggcgcgaa atcgatttg accggattt gccaacttg gagcaaatc atctccgag gcgtttttc gccgtgtct gaggaagtc aagtccgct gcgagctac gctttgggc agcattgcc atcgggaac ttgcagcaa tatcttccg tttatcctg accgaggat gagaacgag ccccgaaga cagtacttg ttgcttcac tctttgaag gaagtcatc gcgtgtctg tcttcaaca ccggagggc atccagcaa ttactgcct ttcgtgccc gcgatttgg gacactttg taccgccac tgcgagagt gaggaggaa gggacaagg aacgtagtt tcggaatgt ttgggcaaa ttgactctg attgacccg aaccatttg ctgccgaag ttgaaggaa tcgctgggc tccgagtcc tcgctcatg cgcacgacg atagtgacg gcgatcaag tttacgatt tctgaccag cctgcttca attgacgca ttgttgcgg caatgcatt ggcgaattt ttgaatact ttggaggat cccgacttg aatgtgaga cgagtggcg cttgtggct ttcaactcg gctgctcac aacaagccc tcgctgatc cgtgatttg cttgacacg attttgccc cgtctatac cacgagact aacattaag cgagaacta atccgtgaa gttgagatg ggcccattc aagcatacc gttgatgac ggcttggac ctgcgcaag gcagcgttc gagtgcatg tacaccttg cttgattct tgtttggac agaatcgat attttcgaa tttatcaat cacgttgaa cttggcctt aaagaccat tacgatatt aaaatgtta acttattta atggtgtcg cgactagct caaatatgc cccggcgcc gtattgcaa agcttagat agactggtc gagcccttg aaggcaact tgtaccatg aaagttaaa gcaaattcg gttaaacag gaatacgag aaacaggat gaacttaag agaagtgcc cttcgggct gttgtttct cttttaagc attcctgat gccgataaa aatcaacac ttgaacgaa tttgtcacc caaatcaag acttctccc gacctagcc cagattttc gactcgatc cagaaagat tccgctaat aatattaac gatttcttt ttgatggat cagagttag
- >TC010870 RB
atggccgaa gaacaacaa cagggtgat gaaagtaac ccttacgag caaggtgat catgctttt gagctagat gatcttgaa cgtgctttg gtaacatgc gaacttcgc agttacgtt gatcaagta gtagaagac gctgcagag atggctagt gtctcttac cagattgcc aatcttctt gagaagatg caatcatct gacaaagat tttcgattt atggccaca aacgattta atggccgaa ctgcagaaa gatagtatc aaattagat gatgattcg gaacgaaag gttgtaaga atgttgttg aaactactt gaagataaa aacggtgaa gtacaaaac ttggctgtc aaatgtctg gggccttta gttaacaaa gttaaggaa ttccaagtg gagaccatt gttgaggcc ttgtgcacc aacatggtc tccgacaag gagcaactg cgggatatt tccagcatt gggctcaag acggttatc gctgaactg ccacaggca cctgggggc ctctccgga aacatttgc aagcggatc accggtcgt ttgacgact gctatcgaa aggcaagac gacgtgtcc gtgcaactg gaagctctc gacattgtc acggatttg ttataccgc tttggcaac gttttgcaa tcctaccat gcctccata ttaaacgcc ctcttacct cagttatgc tcacaacgg caagcagtc cgcaaacgt acgattgca gcttgcagt aacttagtt ctctcgtgc aacaatgca ttgtacgat aaactaatc gagcatctg tacaacggg atgagaact gataacaag aactcccaa attcgcacc tacgtgcag tgcattgca gccgtgtgc cgacaatcg ggccacaag ttcggcgaa catattgaa aagttcgta cctttgatc caaatgtgg agcgctgtg gaagacgac gagaccaga gagttttgt ttacaagct tttgagagt tttatcaat cgttgtccc aaagaaatc accccgaaa atcagcgcc atcacagac ttgtgccta aattacatg actcatgac ccgaattac aactacgac gaagatggg gatgatcga gccatgggg gcttacgat gacgaggaa gaagaggag aatgacgag tacagcgat gacgatgac atgagctgg aaagtgaga cgtgcagct gccaagtgc ttggagtcg atcatctgc actcgccat gaattattg tctgaattt tacaaggtt ttgtcgcct gtgttaatc tcccggttt aaagagcgg gaagagaac gtgaaatca gatattttc cgtgcttac atcgcgctt ttgagacaa acaaaatcg actgttaac gtgaatatc gatccgaac tccatggag caggaggag atgcccatt tacttgctc cagcagcaa gttccaatg attgtgaaa ggcgtgcag tcgcaaatg aaggaaaag agcacgaag acgcgccaa gactgcttc cagctccta aaagaatta tgcaatgtc ctaccgggc gctttaacg caccacatt ggtgatctg attcccggt attctattt tcactgagt gaaaaaaat gggagcagt aacatgaaa attgacggc ttgtcgttt ctcctgtgt cttttggca tcgcaccct cctgaagtt tttcatccc catatcaat atcctccta ccgcacatc atagctaac gtgggtgat aacttttat aaaatcaca gctgaagct ttgaacgtt ctgaaagag ctggtcaag gtcattcgt cctctggac cgcgactcg aacttcgat ttcacgccc tttgtgaag gaaatctac ctctgtact ttatcgcgt ttaaagacg tccgattta gatcaagag gtcaaggaa aaagctatc tcgaccatg gggcaaatt atttgcaac ctgggtgat cacctaggg agtgaattg tcctgctgt cttcctttg ttcctcgat agattacgt aacgaaatc acgaggttg actacagtt aaagcgttg accaaaatt gcgggctct ccattgggc atcgaacta ccgattttg gacgaagca gtgccggtt ttggggctc ttcctgcgt aaaaaccag cgtgcttta aaactaagt actttgcaa ctcattgat tgtttaata aagaattac cacgtctac atgtacgtg gaattactc caacatgta atagtagaa gtgccacca ctccttgac gagtccgac ctgcacata gcccagtac actttggtg atcttgaag tcgattgcg gaataccac cccctggct ttgcaagac ctgccaaca aacatcttc ccgcaaatc ttggtcttg gtgaagtcg cctcttctg caaggttcc gccctgaat tccatgctg gaattcttc aaaactcta gtccaatgc aaccttccg ggattgtcc tacaacgac ttgttgcaa tttctcgtt cacccgatc agcattagc cagggttct ggggccggt gacgccgtc atccacaag caagcctat tactcgttg gccaaatgc gtggctgct ataacagtg acgatgcaa caccaagcg ttgcaaatc gtcccccaa ttcatcaac gaaattcgc tcttcgcgc cacgacgcc cagaagatc ttcactttg ctcgtggtc ggcgaaatc gggcgcgaa atcgatttg accggattt gccaacttg gagcaaatc atctccgag gcgtttttc gccgtgtct gaggaagtc aagtccgct gcgagctac gctttgggc agcattgcc atcgggaac ttgcagcaa tatcttccg tttatcctg accgaggat gagaacgag ccccgaaga cagtacttg ttgcttcac tctttgaag gaagtcatc gcgtgtctg tcttcaaca ccggagggc atccagcaa ttactgcct ttcgtgccc gcgatttgg gacactttg taccgccac tgcgagagt gaggaggaa gggacaagg aacgtagtt tcggaatgt ttgggcaaa ttgactctg attgacccg aaccatttg ctgccgaag ttgaaggaa tcgctgggc tccgagtcc tcgctcatg cgcacgacg atagtgacg gcgatcaag tttacgatt tctgaccag cctgcttca attgacgca ttgttgcgg caatgcatt ggcgaattt ttgaatact ttggaggat cccgacttg aatgtgaga cgagtggcg cttgtggct ttcaactcg gctgctcac aacaagccc tcgctgatc cgtgatttg cttgacacg attttgccc cgtctatac cacgagact aacattaag cgagaacta atccgtgaa gttgagatg ggcccattc aagcatacc gttgatgac ggcttggac ctgcgcaag gcagcgttc gagtgcatg tacaccttg cttgattct tgtttggac agaatcgat attttcgaa tttatcaat cacgttgaa cttggcctt aaagaccat tacgatatt aaaatgtta acttattta atggtgtcg cgactagct caaatatgc cccggcgcc gtattgcaa agcttagat agactggtc gagcccttg aaggcaact tgtaccatg aaagttaaa gcaaattcg gttaaacag gaatacgag aaacaggat gaacttaag agaagtgcc cttcgggct gttgtttct cttttaagc attcctgat gccgataaa aatcaacac ttgaacgaa tttgtcacc caaatcaag acttctccc gacctagcc cagattttc gactcgatc cagaaagat tccgctaat aatattaac gatttcttt ttgatggat cagagttag
- >TC010870 RA
tagctgtct taaatctca attcatcta gtgcattag agtgtgaaa ttgaataaa aactgttgt tttgtcaag gttttggat tacgtgagc caacctgag cttgggatc ccccgcaca gaggtgaag aaatccgac tgtaaaggt aagccacga aattttttg acactacta acttcgcaa caaatcaag acttttgtc gatttcggt agatttcta aagtggcaa caaatgacg aacttacac acgatatga tttagcttt atttccttt tagcaaaac gataaattt tggatttaa acggtgtgt gcttgatca tacaaaatg gccgaagaa caacaacag ggtgatgaa agtaaccct tacgagcaa ggtgatcat gcttttgag ctagatgat cttgaacgt gctttggta acatgcgag taagtattc acgggtttg aatttggcg ctagattct atgaatgcg cacgcgtag tacggtccc acttaattg tatttttat gtctttccg gtacacacg aagtgacga taataggtg ttttcggct tattcaaaa tcttgtgtg tttagttta ttccaaaat tccacaatt ttgaaatat tggaaggtt cccaaccgc acgcctatg gcgccgctt tggagtgta gtggggatt taatttgtt attaaaata tcggaattt actatatga tttgaaaaa tatcaatta ctgtacaaa ataacgcgt aaatgggtg caaattttt gtcaaatca ctttagccg gtataacaa taaatcgca gttggtact ccggtgtct gaggttgtc attattatt gtggtggat tgatataag ctagtgaat aataaaaat ttccaaatt ttcattatt agtgatatt ataagtgta attattgtg atttactta ctttgttag tgatagctg tatttcatg tctaatttg tacatgcag aacgacaca catagtaaa ttatttaca attttcgta ctctaacct aaaagaact agatgaaat gctcatata aattcagtc ttacaataa aaaactgtt ttatttaac atcaatttg cttaaagac ttcgcagtt acgttgatc aagtagtag aagacgctg cagagatgg ctagtgtct cttaccaga ttgccaatc ttcttgaga agatgcaat catctgaca aagattttc gatttatgg ccacaaacg atttaatgg ccgaactgc agaaagata gtatcaaat tagatgatg attcggaac gaaaggttg taagaatgt tgttgaaac tacttgaag ataaaaacg gtgaagtac aaaacttgg ctgtcaaat gtctggggc ctttagtta acaaagtta aggaattcc aagtggaga ccattgttg aggccttgt gcaccaaca tggtctccg acaaggagc aactgcggg atatttcca gcattgggc tcaagacgg ttatcgctg aactgccac aggcacctg ggggcctct ccggaaaca tttgcaagc ggatcaccg gtcgtttga cgactgcta tcgaaaggc aagacgacg tgtccgtgc aactggaag ctctcgaca ttgtcacgg atttgttat accgctttg gcaacgttt tgcaatcct accatgcct ccatattaa acgccctct tacctcagt tatgctcac aacggcaag cagtccgca aacgtacga ttgcagctt gcagtaact tagttctct cgtgcaaca atgcattgt acgataaac taatcgagc atctgtaca acgggatga gaactgata acaagaact cccaaattc gcacctacg tgcagtgca ttgcagccg tgtgccgac aatcgggcc acaagttcg gcgaacata ttgaaaagt tcgtacctt tgatccaaa tgtggagcg ctgtggaag acgacgaga ccagagagt tttgtttac aagcttttg agagtttta tcaatcgtt gtcccaaag aaatcaccc cgaaaatca gcgccatca cagacttgt gcctaaatt acatgactc atgacccga attacaact acgacgaag atggggatg atcgagcca tgggggctt acgatgacg aggaagaag aggagaatg acgagtaca gcgatgacg atgacatga gctggaaag tgagacgtg cagctgcca agtgcttgg agtcgatca tctgcactc gccatgaat tattgtctg aattttaca aggttttgt cgcctgtgt taatctccc ggtttaaag agcgggaag agaacgtga aatcagata ttttccgtg cttacatcg cgcttttga gacaaacaa aatcgactg ttaacgtga atatcgatc cgaactcca tggagcagg aggagatgc ccatttact tgctccagc agcaagttc caatgattg tgaaaggcg tgcagtcgc aaatgaagg aaaagagca cgaagacgc gccaagact gcttccagc tcctaaaag aattatgca atgtcctac cgggcgctt taacgcacc acattggtg atctgattc ccggtattc tattttcac tgagtgaaa aaaatggga gcagtaaca tgaaaattg acggcttgt cgtttctcc tgtgtcttt tggcatcgc accctcctg aagtttttc atccccata tcaatatcc tcctaccgc acatcatag ctaacgtgg gtgataact tttataaaa tcacagctg aagctttga acgttctga aagagctgg tcaaggtca ttcgtcctc tggaccgcg actcgaact tcgatttca cgccctttg tgaaggaaa tctacctct gtactttat cgcgtttaa agacgtccg atttagatc aagaggtca aggaaaaag ctatctcga ccatggggc aaattattt gcaacctgg gtgatcacc tagggagtg aattgtcct gctgtcttc ctttgttcc tcgatagat tacgtaacg aaatcacga ggttgacta cagttaaag cgttgacca aaattgcgg gctctccat tgggcatcg aactaccga ttttggacg aagcagtgc cggttttgg ggctcttcc tgcgtaaaa accagcgtg ctttaaaac taagtactt tgcaactca ttgattgtt taataaaga attaccacg tctacatgt acgtggaat tactccaac atgtaatag tagaagtgc caccactcc ttgacgagt ccgacctgc acatagccc agtacactt tggtgatct tgaagtcga ttgcggaat accaccccc tggctttgc aagacctgc caacaaaca tcttcccgc aaatcttgg tcttggtga agtcgcctc ttctgcaag gttccgccc tgaattcca tgctggaat tcttcaaaa ctctagtcc aatgcaacc ttccgggat tgtcctaca acgacttgt tgcaatttc tcgttcacc cgatcagca ttagccagg gttctgggg ccggtgacg ccgtcatcc acaagcaag cctattact cgttggcca aatgcgtgg ctgctataa cagtgacga tgcaacacc aagcgttgc aaatcgtcc cccaattca tcaacgaaa ttcgctctt cgcgccacg acgcccaga agatcttca ctttgctcg tggtcggcg aaatcgggc gcgaaatcg atttgaccg gatttgcca acttggagc aaatcatct ccgaggcgt ttttcgccg tgtctgagg aagtcaagt ccgctgcga gctacgctt tgggcagca ttgccatcg ggaacttgc agcaatatc ttccgttta tcctgaccg aggatgaga acgagcccc gaagacagt acttgttgc ttcactctt tgaaggaag tcatcgcgt gtctgtctt caacaccgg agggcatcc agcaattac tgcctttcg tgcccgcga tttgggaca ctttgtacc gccactgcg agagtgagg aggaaggga caaggaacg tagtttcgg aatgtttgg gcaaattga ctctgattg acccgaacc atttgctgc cgaagttga aggaatcgc tgggctccg agtcctcgc tcatgcgca cgacgatag tgacggcga tcaagttta cgatttctg accagcctg cttcaattg acgcattgt tgcggcaat gcattggcg aatttttga atactttgg aggatcccg acttgaatg tgagacgag tggcgcttg tggctttca actcggctg ctcacaaca agccctcgc tgatccgtg atttgcttg acacgattt tgccccgtc tataccacg agactaaca ttaagcgag aactaatcc gtgaagttg agatgggcc cattcaagc ataccgttg atgacggct tggacctgc gcaaggcag cgttcgagt gcatgtaca ccttgcttg attcttgtt tggacagaa tcgatattt tcgaattta tcaatcacg ttgaacttg gccttaaag accattacg atattaaaa tgttaactt atttaatgg tgtcgcgac tagctcaaa tatgccccg gcgccgtat tgcaaagct tagatagac tggtcgagc ccttgaagg caacttgta ccatgaaag ttaaagcaa attcggtta aacaggaat acgagaaac aggatgaac ttaagagaa gtgcccttc gggctgttg tttctcttt taagcattc ctgatgccg ataaaaatc aacacttga acgaatttg tcacccaaa tcaagactt ctcccgacc tagcccaga ttttcgact cgatccaga aagattccg ctaataata ttaacgatt tctttttga tggatcaga gttagacat tttcaaagt tactatttt ttcgacgtt tgttcaatt ttttgttct agttcattt tcgttgtgt ttcatttgt tcattcata tttttcata ttttttcaa atctatgta attgatctg aattaataa agctgtgtt tcaaca
- >TC010870 RB
tagctgtct taaatctca attcatcta gtgcattag agtgtgaaa ttgaataaa aactgttgt tttgtcaag gttttggat tacgtgagc caacctgag cttgggatc ccccgcaca gaggtgaag aaatccgac tgtaaaggt aagccacga aattttttg acactacta acttcgcaa caaatcaag acttttgtc gatttcggt agatttcta aagtggcaa caaatgacg aacttacac acgatatga tttagcttt atttccttt tagcaaaac gataaattt tggatttaa acggtgtgt gcttgatca tacaaaatg gccgaagaa caacaacag ggtgatgaa agtaaccct tacgagcaa ggtgatcat gcttttgag ctagatgat cttgaacgt gctttggta acatgcgaa cttcgcagt tacgttgat caagtagta gaagacgct gcagagatg gctagtgtc tcttaccag attgccaat cttcttgag aagatgcaa tcatctgac aaagatttt cgatttatg gccacaaac gatttaatg gccgaactg cagaaagat agtatcaaa ttagatgat gattcggaa cgaaaggtt gtaagaatg ttgttgaaa ctacttgaa gataaaaac ggtgaagta caaaacttg gctgtcaaa tgtctgggg cctttagtt aacaaagtt aaggaattc caagtggag accattgtt gaggccttg tgcaccaac atggtctcc gacaaggag caactgcgg gatatttcc agcattggg ctcaagacg gttatcgct gaactgcca caggcacct gggggcctc tccggaaac atttgcaag cggatcacc ggtcgtttg acgactgct atcgaaagg caagacgac gtgtccgtg caactggaa gctctcgac attgtcacg gatttgtta taccgcttt ggcaacgtt ttgcaatcc taccatgcc tccatatta aacgccctc ttacctcag ttatgctca caacggcaa gcagtccgc aaacgtacg attgcagct tgcagtaac ttagttctc tcgtgcaac aatgcattg tacgataaa ctaatcgag catctgtac aacgggatg agaactgat aacaagaac tcccaaatt cgcacctac gtgcagtgc attgcagcc gtgtgccga caatcgggc cacaagttc ggcgaacat attgaaaag ttcgtacct ttgatccaa atgtggagc gctgtggaa gacgacgag accagagag ttttgttta caagctttt gagagtttt atcaatcgt tgtcccaaa gaaatcacc ccgaaaatc agcgccatc acagacttg tgcctaaat tacatgact catgacccg aattacaac tacgacgaa gatggggat gatcgagcc atgggggct tacgatgac gaggaagaa gaggagaat gacgagtac agcgatgac gatgacatg agctggaaa gtgagacgt gcagctgcc aagtgcttg gagtcgatc atctgcact cgccatgaa ttattgtct gaattttac aaggttttg tcgcctgtg ttaatctcc cggtttaaa gagcgggaa gagaacgtg aaatcagat attttccgt gcttacatc gcgcttttg agacaaaca aaatcgact gttaacgtg aatatcgat ccgaactcc atggagcag gaggagatg cccatttac ttgctccag cagcaagtt ccaatgatt gtgaaaggc gtgcagtcg caaatgaag gaaaagagc acgaagacg cgccaagac tgcttccag ctcctaaaa gaattatgc aatgtccta ccgggcgct ttaacgcac cacattggt gatctgatt cccggtatt ctattttca ctgagtgaa aaaaatggg agcagtaac atgaaaatt gacggcttg tcgtttctc ctgtgtctt ttggcatcg caccctcct gaagttttt catccccat atcaatatc ctcctaccg cacatcata gctaacgtg ggtgataac ttttataaa atcacagct gaagctttg aacgttctg aaagagctg gtcaaggtc attcgtcct ctggaccgc gactcgaac ttcgatttc acgcccttt gtgaaggaa atctacctc tgtacttta tcgcgttta aagacgtcc gatttagat caagaggtc aaggaaaaa gctatctcg accatgggg caaattatt tgcaacctg ggtgatcac ctagggagt gaattgtcc tgctgtctt cctttgttc ctcgataga ttacgtaac gaaatcacg aggttgact acagttaaa gcgttgacc aaaattgcg ggctctcca ttgggcatc gaactaccg attttggac gaagcagtg ccggttttg gggctcttc ctgcgtaaa aaccagcgt gctttaaaa ctaagtact ttgcaactc attgattgt ttaataaag aattaccac gtctacatg tacgtggaa ttactccaa catgtaata gtagaagtg ccaccactc cttgacgag tccgacctg cacatagcc cagtacact ttggtgatc ttgaagtcg attgcggaa taccacccc ctggctttg caagacctg ccaacaaac atcttcccg caaatcttg gtcttggtg aagtcgcct cttctgcaa ggttccgcc ctgaattcc atgctggaa ttcttcaaa actctagtc caatgcaac cttccggga ttgtcctac aacgacttg ttgcaattt ctcgttcac ccgatcagc attagccag ggttctggg gccggtgac gccgtcatc cacaagcaa gcctattac tcgttggcc aaatgcgtg gctgctata acagtgacg atgcaacac caagcgttg caaatcgtc ccccaattc atcaacgaa attcgctct tcgcgccac gacgcccag aagatcttc actttgctc gtggtcggc gaaatcggg cgcgaaatc gatttgacc ggatttgcc aacttggag caaatcatc tccgaggcg tttttcgcc gtgtctgag gaagtcaag tccgctgcg agctacgct ttgggcagc attgccatc gggaacttg cagcaatat cttccgttt atcctgacc gaggatgag aacgagccc cgaagacag tacttgttg cttcactct ttgaaggaa gtcatcgcg tgtctgtct tcaacaccg gagggcatc cagcaatta ctgcctttc gtgcccgcg atttgggac actttgtac cgccactgc gagagtgag gaggaaggg acaaggaac gtagtttcg gaatgtttg ggcaaattg actctgatt gacccgaac catttgctg ccgaagttg aaggaatcg ctgggctcc gagtcctcg ctcatgcgc acgacgata gtgacggcg atcaagttt acgatttct gaccagcct gcttcaatt gacgcattg ttgcggcaa tgcattggc gaatttttg aatactttg gaggatccc gacttgaat gtgagacga gtggcgctt gtggctttc aactcggct gctcacaac aagccctcg ctgatccgt gatttgctt gacacgatt ttgccccgt ctataccac gagactaac attaagcga gaactaatc cgtgaagtt gagatgggc ccattcaag cataccgtt gatgacggc ttggacctg cgcaaggca gcgttcgag tgcatgtac accttgctt gattcttgt ttggacaga atcgatatt ttcgaattt atcaatcac gttgaactt ggccttaaa gaccattac gatattaaa atgttaact tatttaatg gtgtcgcga ctagctcaa atatgcccc ggcgccgta ttgcaaagc ttagataga ctggtcgag cccttgaag gcaacttgt accatgaaa gttaaagca aattcggtt aaacaggaa tacgagaaa caggatgaa cttaagaga agtgccctt cgggctgtt gtttctctt ttaagcatt cctgatgcc gataaaaat caacacttg aacgaattt gtcacccaa atcaagact tctcccgac ctagcccag attttcgac tcgatccag aaagattcc gctaataat attaacgat ttctttttg atggatcag agttagaca ttttcaaag ttactattt tttcgacgt ttgttcaat tttttgttc tagttcatt ttcgttgtg tttcatttg ttcattcat atttttcat attttttca aatctatgt aattgatct gaattaata aagctgtgt ttcaaca
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