| General information | |||
| TC010869 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 5080872..5094024 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010869 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01753 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
Days post injection
- >TC010869 RA
MKGKGIGEN NLINVIKDS KRISIFNLE NPTIFEDYY LFTHSGVSK RSLSLNSEN RAKLAVDTR VRWSKQQTA KKRVKRDLR LQDSDPKWP SMWYLNRGN GLDMNVIPA WLEGITGKG AVVTILDDG LEKDHPDLV QNYDPLASY DVNSHDSDP SPRYDMIDS NRHGTRCAG EVAATSNNS VCALGVAHG AQVGGVRML DGDVTDAVE ARSLSLNPQ HIDIYSASW GPDDDGKTV DGPGELATR AFIEGVTKG RNGKGSIFV WASGNGGRD HDNCNCDGY TNSIYSLSI SSATEHGHV PWYSEACSS TLASTYSSG AVGERQVVT TDLRYSCTS SHTGTSASA PLAAGICAL ALEANPNLT WRDMQHIVV RTARPENLI APDWQTNGV GRNVSHSFG YGLMDAYAM VQLSRNWIT VPEQHKCEI TAPHVQRPI PAKSVVVLQ LQVKECEGV EVLEHVQAK LTIYSQRRG DLNIQLTSP MGTRVTLLA HRAHDTSRV GFSHWPFMS VHSWGETPF GTWQLEIHN DGRLLANVT AWDLTLYGT ATPPAADLK QETVHQPSI VDIYPEEID DNSIQDASA NSLKFGAEV KKDTSGCLK TNGQLCLDT LYVLLIYTI SSLIFIFDL YLNIRIKSC HIKRSIIEF HNVNRSYNL YCIHSTTNQ NKENSDNNF HRDTRKNKC KVTRHIFKD VFLNRTFRD FLGKVGNLK VFLRIMYHN VREWLHRLN TLRLVVLFC DISTFYIFF KLKCSILCM LPSYSEVSN
- >TC010869 RB
MKGKGIGEN NLINVIKDS KRISIFNLE NPTIFEDYY LFTHSGVSK RSLSLNSEN RAKLAVDTR VRWSKQQTA KKRVKRDLR LQDSDPKWP SMWYLNRGN GLDMNVIPA WLEGITGKG AVVTILDDG LEKDHPDLV QNYDPLASY DVNSHDSDP SPRYDMIDS NRHGTRCAG EVAATSNNS VCALGVAHG AQVGGVRML DGDVTDAVE ARSLSLNPQ HIDIYSASW GPDDDGKTV DGPGELATR AFIEGVTKG RNGKGSIFV WASGNGGRD HDNCNCDGY TNSIYSLSI SSATEHGHV PWYSEACSS TLASTYSSG AVGERQVVT TDLRYSCTS SHTGTSASA PLAAGICAL ALEANPNLT WRDMQHIVV RTARPENLI APDWQTNGV GRNVSHSFG YGLMDAYAM VQLSRNWIT VPEQHKCEI TAPHVQRPI PAKSVVVLQ LQVKECEGV EVLEHVQAK LTIYSQRRG DLNIQLTSP MGTRVTLLA HRAHDTSRV GFSHWPFMS VHSWGETPF GTWQLEIHN DGRLLGRAT LQNWSLVLY GTKQFLPPL SNNNIETNK VNKTKTNNI FKKKNSKKQ KNKNLQKVT TTKPPKSQY KLKLKNTTP KISVPNVTN SKNRKIAKT TTEFSPYFY AINTSKYKN SEDSVKQYL KFVKNITIT YPVMIPSRE MSKPRESFK KSQKQKEKS RDINSNRNA KVFTSKTTE LTTYQTTTS SPRGRLANV TAWDLTLYG TATPPAADL KQETVHQPS IVDIYPEEI DDNSIQDAS ANSLKFGAE VKKDTSGCL KTNGQLCLG CPENLFLHK GRCIKSCPD GFYHLTLRR ERPSEAEDL SPENNTNRC LPCHYTCKK CTGPVDYQC SECFPDAQL VLVSSTETF CYSASVASS IVADKWYIR VFVMLLIVS VLLVLHLLW QIFKSRKRP RGGYLRFNL INNSDEKDV KMAVYSDSE
- >TC010869 RC
MKGKGIGEN NLINVIKDS KRISIFNLE NPTIFEDYY LFTHSGVSK RSLSLNSEN RAKLAVDTR VRWSKQQTA KKRVKRDLR LQDSDPKWP SMWYLNRGN GLDMNVIPA WLEGITGKG AVVTILDDG LEKDHPDLV QNYDPLASY DVNSHDSDP SPRYDMIDS NRHGTRCAG EVAATSNNS VCALGVAHG AQVGGVRML DGDVTDAVE ARSLSLNPQ HIDIYSASW GPDDDGKTV DGPGELATR AFIEGVTKG RNGKGSIFV WASGNGGRD HDNCNCDGY TNSIYSLSI SSATEHGHV PWYSEACSS TLASTYSSG AVGERQVVT TDLRYSCTS SHTGTSASA PLAAGICAL ALEANPNLT WRDMQHIVV RTARPENLI APDWQTNGV GRNVSHSFG YGLMDAYAM VQLSRNWIT VPEQHKCEI TAPHVQRPI PAKSVVVLQ LQVKECEGV EVLEHVQAK LTIYSQRRG DLNIQLTSP MGTRVTLLA HRAHDTSRV GFSHWPFMS VHSWGETPF GTWQLEIHN DGRLLGRAT LQNWSLVLY GTKQFLPPL SNNNIETNK VNKTKTNNI FKKKNSKKQ KNKNLQKVT TTKPPKSQY KLKLKNTTP KISVPNVTN SKNRKIAKT TTEFSPYFY AINTSKYKN SEDSVKQYL KFVKNITIT YPVMIPSRE MSKPRESFK KSQKQKEKS RDINSNRNA KVFTSKTTE LTTYQTTTS SPRGRLANV TAWDLTLYG TATPPAADL KQETVHQPS IVDIYPEEI DDNSIQDAS ANSLKFGAE VKKDTSGCL KTNGQLCLD TLYVLLIYT ISSLIFIFD LYLNIRIKS CHIKRSIIE FHNVNRSYN LYCIHSTTN QNKENSDNN FHRDTRKNK CKVTRHIFK DVFLNRTFR DFLGKVGNL KVFLRIMYH NVREWLHRL NTLRLVVLF CDISTFYIF FKLKCSILC MLPSYSEVS N
- >TC010869 RA
atgaagggg aaaggtatt ggggaaaac aacttgatc aacgttatt aaagatagc aaacgcatc tcaattttc aacttggaa aatcctacg attttcgag gactactac ctcttcacc cacagcggg gtctccaaa cgatccctc agcttgaat tccgaaaat cgggcaaaa ctagcagtc gacaccagg gtcaggtgg tcaaaacaa caaacggca aagaaacga gttaaaaga gacctaaga ctgcaagac tcagacccc aaatggccc agcatgtgg tatctgaac cgaggaaat ggtctagat atgaacgtt attcccgcc tggctagag ggcataacc ggcaaaggc gccgtcgta accattcta gacgatggt ctcgagaag gaccatcca gatctagta caaaattac gaccccttg gcctcatac gacgtcaac agtcacgat tctgatcct agtccaaga tacgatatg atagactca aaccggcac ggcactcgc tgtgctggt gaagtggct gcgactagt aataactcg gtttgtgct ctgggtgtt gcccacggt gcccaagtg ggcggcgtg cggatgctg gatggggat gtaaccgac gcggtggag gctcgctcc ctgagcctc aacccgcag cacatcgac atctacagc gcgtcttgg ggccccgac gacgacggc aaaaccgtc gacgggccc ggggagctc gccacgcgg gcgttcatc gaaggggtg acaaagggc cgtaatggg aaaggttcg atttttgtg tgggcgtcg gggaacggc ggccgggat cacgataat tgcaactgt gacgggtac acgaattcg atctatagt ttgtcgata tcgagtgcg acggaacac ggacacgtt ccgtggtac agtgaggcg tgcagttcc acccttgcc tcgacttac agtagcggg gctgtgggg gaacgccag gtggtgacc actgatttg aggtattct tgcactagt agccacacg gggaccagt gcctcggcg ccgcttgca gcgggcatc tgtgcactg gctctggag gccaatccc aacctgacc tggagggac atgcaacac attgttgtt cggaccgcc aggcccgaa aatctgatc gcccccgac tggcagacc aacggcgtg gggcgaaat gtctcacat agttttggc tatgggctt atggacgcc tacgccatg gtacaactc tcgaggaat tggatcaca gtgcctgaa caacacaaa tgcgaaatc actgcgcct cacgtccaa aggcctatt cctgccaaa agcgtagtc gttttacag ttacaagta aaggagtgt gagggagtt gaagtctta gaacacgtc caagctaaa ttaactatc tattcgcaa agacgtggc gacctgaat attcagttg acctcccct atgggaacg cgggtaaca ctattagcc catagggcc cacgacact tccagggtt gggttctcc cattggcct ttcatgtca gtgcattcc tggggagag actcctttc ggaacctgg cagttggaa atacataac gacggacgc cttcttgcg aacgtgact gcctgggat ttgactctg tatggcacg gccacaccg ccggccgcc gacctgaag caggagacg gtgcaccag ccctccatt gtggacata taccccgaa gagatcgat gataattcg attcaagac gcgtcggcc aattccctc aagtttggt gcagaggtt aagaaagat acgtccggt tgcttaaaa actaatggc cagttatgt ttagacact ctctatgtc ctattaata tatactatc agttcacta attttcatt ttcgacttg tatttaaac attcggatc aagtcttgc cacattaaa cgtagcatc attgagttt cataatgta aacagatca tacaatctt tactgtatt cattcaacc acaaatcaa aacaaagaa aattctgac aacaatttt cacagagac acacggaaa aacaaatgt aaagtcacg cgacatata tttaaagac gtttttttg aatcgtacg tttagagac tttttaggg aaggtaggg aatttaaag gtttttttg cgaataatg tatcataac gttcgcgaa tggttacat agactaaat acgcttaga ttagtagta ctattttgt gatatctca acgttttat atatttttt aagctaaag tgttcaata ttatgtatg ttaccgtct tattcggaa gtatccaac tga
- >TC010869 RB
atgaagggg aaaggtatt ggggaaaac aacttgatc aacgttatt aaagatagc aaacgcatc tcaattttc aacttggaa aatcctacg attttcgag gactactac ctcttcacc cacagcggg gtctccaaa cgatccctc agcttgaat tccgaaaat cgggcaaaa ctagcagtc gacaccagg gtcaggtgg tcaaaacaa caaacggca aagaaacga gttaaaaga gacctaaga ctgcaagac tcagacccc aaatggccc agcatgtgg tatctgaac cgaggaaat ggtctagat atgaacgtt attcccgcc tggctagag ggcataacc ggcaaaggc gccgtcgta accattcta gacgatggt ctcgagaag gaccatcca gatctagta caaaattac gaccccttg gcctcatac gacgtcaac agtcacgat tctgatcct agtccaaga tacgatatg atagactca aaccggcac ggcactcgc tgtgctggt gaagtggct gcgactagt aataactcg gtttgtgct ctgggtgtt gcccacggt gcccaagtg ggcggcgtg cggatgctg gatggggat gtaaccgac gcggtggag gctcgctcc ctgagcctc aacccgcag cacatcgac atctacagc gcgtcttgg ggccccgac gacgacggc aaaaccgtc gacgggccc ggggagctc gccacgcgg gcgttcatc gaaggggtg acaaagggc cgtaatggg aaaggttcg atttttgtg tgggcgtcg gggaacggc ggccgggat cacgataat tgcaactgt gacgggtac acgaattcg atctatagt ttgtcgata tcgagtgcg acggaacac ggacacgtt ccgtggtac agtgaggcg tgcagttcc acccttgcc tcgacttac agtagcggg gctgtgggg gaacgccag gtggtgacc actgatttg aggtattct tgcactagt agccacacg gggaccagt gcctcggcg ccgcttgca gcgggcatc tgtgcactg gctctggag gccaatccc aacctgacc tggagggac atgcaacac attgttgtt cggaccgcc aggcccgaa aatctgatc gcccccgac tggcagacc aacggcgtg gggcgaaat gtctcacat agttttggc tatgggctt atggacgcc tacgccatg gtacaactc tcgaggaat tggatcaca gtgcctgaa caacacaaa tgcgaaatc actgcgcct cacgtccaa aggcctatt cctgccaaa agcgtagtc gttttacag ttacaagta aaggagtgt gagggagtt gaagtctta gaacacgtc caagctaaa ttaactatc tattcgcaa agacgtggc gacctgaat attcagttg acctcccct atgggaacg cgggtaaca ctattagcc catagggcc cacgacact tccagggtt gggttctcc cattggcct ttcatgtca gtgcattcc tggggagag actcctttc ggaacctgg cagttggaa atacataac gacggacgc cttcttggt cgagcgaca cttcagaac tggtctttg gtcctgtat gggaccaag cagtttctc cctccttta tctaataat aatatagaa actaacaaa gtcaacaaa actaaaaca aataatata tttaaaaag aaaaactcg aaaaagcaa aaaaacaaa aacttgcag aaagtcacg acaactaaa ccccccaaa tcccaatac aaactaaag ttaaagaac acaacgccg aaaatcagc gttcctaac gtaacaaat tcgaaaaat cgcaaaata gccaagacc acgaccgag ttctcgccc tacttttac gcgataaac accagtaaa tacaaaaat tcggaggac agtgttaag cagtacttg aaatttgtt aagaacatt acgataact tacccggtc atgattccc tcccgcgag atgtcgaaa cctcgcgaa tcgttcaaa aaatcgcaa aaacagaaa gagaaaagc cgcgatatt aactcgaac cgcaacgca aaagttttc acttctaaa acgactgaa ttaacaacc tatcaaact accaccagt tccccccga ggaagattg gcgaacgtg actgcctgg gatttgact ctgtatggc acggccaca ccgccggcc gccgacctg aagcaggag acggtgcac cagccctcc attgtggac atatacccc gaagagatc gatgataat tcgattcaa gacgcgtcg gccaattcc ctcaagttt ggtgcagag gttaagaaa gatacgtcc ggttgctta aaaactaat ggccagtta tgtttaggc tgtccggaa aatttattt ttacacaag ggtcgatgt atcaaaagt tgtcctgac ggcttttac catttgacg cttaggcgc gagaggcct tcggaggcc gaagattta tctccggaa aataacacc aatcggtgc ttaccatgc cattacact tgtaagaag tgcacaggc ccggtggac taccagtgt tccgagtgt ttcccagat gcccaactg gtgctggtt tccagcacc gaaactttt tgttactcc gcatctgtg gcctcttca atagtagca gataaatgg tatataagg gtatttgtt atgttactt atagttagc gttttatta gtattgcat ttattgtgg caaattttt aaatcaagg aaacggccc cgcggaggg tacttgcgt tttaatctg ataaacaat agtgatgaa aaagatgta aaaatggct gtttatagt gatagcgaa taa
- >TC010869 RC
atgaagggg aaaggtatt ggggaaaac aacttgatc aacgttatt aaagatagc aaacgcatc tcaattttc aacttggaa aatcctacg attttcgag gactactac ctcttcacc cacagcggg gtctccaaa cgatccctc agcttgaat tccgaaaat cgggcaaaa ctagcagtc gacaccagg gtcaggtgg tcaaaacaa caaacggca aagaaacga gttaaaaga gacctaaga ctgcaagac tcagacccc aaatggccc agcatgtgg tatctgaac cgaggaaat ggtctagat atgaacgtt attcccgcc tggctagag ggcataacc ggcaaaggc gccgtcgta accattcta gacgatggt ctcgagaag gaccatcca gatctagta caaaattac gaccccttg gcctcatac gacgtcaac agtcacgat tctgatcct agtccaaga tacgatatg atagactca aaccggcac ggcactcgc tgtgctggt gaagtggct gcgactagt aataactcg gtttgtgct ctgggtgtt gcccacggt gcccaagtg ggcggcgtg cggatgctg gatggggat gtaaccgac gcggtggag gctcgctcc ctgagcctc aacccgcag cacatcgac atctacagc gcgtcttgg ggccccgac gacgacggc aaaaccgtc gacgggccc ggggagctc gccacgcgg gcgttcatc gaaggggtg acaaagggc cgtaatggg aaaggttcg atttttgtg tgggcgtcg gggaacggc ggccgggat cacgataat tgcaactgt gacgggtac acgaattcg atctatagt ttgtcgata tcgagtgcg acggaacac ggacacgtt ccgtggtac agtgaggcg tgcagttcc acccttgcc tcgacttac agtagcggg gctgtgggg gaacgccag gtggtgacc actgatttg aggtattct tgcactagt agccacacg gggaccagt gcctcggcg ccgcttgca gcgggcatc tgtgcactg gctctggag gccaatccc aacctgacc tggagggac atgcaacac attgttgtt cggaccgcc aggcccgaa aatctgatc gcccccgac tggcagacc aacggcgtg gggcgaaat gtctcacat agttttggc tatgggctt atggacgcc tacgccatg gtacaactc tcgaggaat tggatcaca gtgcctgaa caacacaaa tgcgaaatc actgcgcct cacgtccaa aggcctatt cctgccaaa agcgtagtc gttttacag ttacaagta aaggagtgt gagggagtt gaagtctta gaacacgtc caagctaaa ttaactatc tattcgcaa agacgtggc gacctgaat attcagttg acctcccct atgggaacg cgggtaaca ctattagcc catagggcc cacgacact tccagggtt gggttctcc cattggcct ttcatgtca gtgcattcc tggggagag actcctttc ggaacctgg cagttggaa atacataac gacggacgc cttcttggt cgagcgaca cttcagaac tggtctttg gtcctgtat gggaccaag cagtttctc cctccttta tctaataat aatatagaa actaacaaa gtcaacaaa actaaaaca aataatata tttaaaaag aaaaactcg aaaaagcaa aaaaacaaa aacttgcag aaagtcacg acaactaaa ccccccaaa tcccaatac aaactaaag ttaaagaac acaacgccg aaaatcagc gttcctaac gtaacaaat tcgaaaaat cgcaaaata gccaagacc acgaccgag ttctcgccc tacttttac gcgataaac accagtaaa tacaaaaat tcggaggac agtgttaag cagtacttg aaatttgtt aagaacatt acgataact tacccggtc atgattccc tcccgcgag atgtcgaaa cctcgcgaa tcgttcaaa aaatcgcaa aaacagaaa gagaaaagc cgcgatatt aactcgaac cgcaacgca aaagttttc acttctaaa acgactgaa ttaacaacc tatcaaact accaccagt tccccccga ggaagattg gcgaacgtg actgcctgg gatttgact ctgtatggc acggccaca ccgccggcc gccgacctg aagcaggag acggtgcac cagccctcc attgtggac atatacccc gaagagatc gatgataat tcgattcaa gacgcgtcg gccaattcc ctcaagttt ggtgcagag gttaagaaa gatacgtcc ggttgctta aaaactaat ggccagtta tgtttagac actctctat gtcctatta atatatact atcagttca ctaattttc attttcgac ttgtattta aacattcgg atcaagtct tgccacatt aaacgtagc atcattgag tttcataat gtaaacaga tcatacaat ctttactgt attcattca accacaaat caaaacaaa gaaaattct gacaacaat tttcacaga gacacacgg aaaaacaaa tgtaaagtc acgcgacat atatttaaa gacgttttt ttgaatcgt acgtttaga gacttttta gggaaggta gggaattta aaggttttt ttgcgaata atgtatcat aacgttcgc gaatggtta catagacta aatacgctt agattagta gtactattt tgtgatatc tcaacgttt tatatattt tttaagcta aagtgttca atattatgt atgttaccg tcttattcg gaagtatcc aactga
- >TC010869 RA
cggctccga aaacatgaa aacaaccgc agcattgtg agcacaatg ttataagga cctcgatac tagaaaatt cagcaaagc gatgaaggg gaaaggtat tggggaaaa caacttgat caacgttat taaagatag caaacgcat ctcaatttt caacttgga aaatcctac gattttcga ggactacta cctcttcac ccacagcgg ggtctccaa acgatccct cagcttgaa ttccgaaaa tcgggcaaa actagcagt cgacaccag ggtcaggtg gtcaaaaca acaaacggc aaagaaacg agttaaaag agacctaag actgcaaga ctcagaccc caaatggcc cagcatgtg gtatctgaa ccgaggaaa tggtctaga tatgaacgt tattcccgc ctggctaga gggcataac cggcaaagg cgccgtcgt aaccattct agacgatgg tctcgagaa ggaccatcc agatctagt acaaaatta cgacccctt ggcctcata cgacgtcaa cagtcacga ttctgatcc tagtccaag atacgatat gatagactc aaaccggca cggcactcg ctgtgctgg tgaagtggc tgcgactag taataactc ggtttgtgc tctgggtgt tgcccacgg tgcccaagt gggcggcgt gcggatgct ggatgggga tgtaaccga cgcggtgga ggctcgctc cctgagcct caacccgca gcacatcga catctacag cgcgtcttg gggccccga cgacgacgg caaaaccgt cgacgggcc cggggagct cgccacgcg ggcgttcat cgaaggggt gacaaaggg ccgtaatgg gaaaggttc gatttttgt gtgggcgtc ggggaacgg cggccggga tcacgataa ttgcaactg tgacgggta cacgaattc gatctatag tttgtcgat atcgagtgc gacggaaca cggacacgt tccgtggta cagtgaggc gtgcagttc cacccttgc ctcgactta cagtagcgg ggctgtggg ggaacgcca ggtggtgac cactgattt gaggtattc ttgcactag tagccacac ggggaccag tgcctcggc gccgcttgc agcgggcat ctgtgcact ggctctgga ggccaatcc caacctgac ctggaggga catgcaaca cattgttgt tcggaccgc caggcccga aaatctgat cgcccccga ctggcagac caacggcgt ggggcgaaa tgtctcaca tagttttgg ctatgggct tatggacgc ctacgccat ggtacaact ctcgaggaa ttggatcac agtgcctga acaacacaa atgcgaaat cactgcgcc tcacgtcca aaggcctat tcctgccaa aagcgtagt cgttttaca gttacaagt aaaggagtg tgagggagt tgaagtctt agaacacgt ccaagctaa attaactat ctattcgca aagacgtgg cgacctgaa tattcagtt gacctcccc tatgggaac gcgggtaac actattagc ccatagggc ccacgacac ttccagggt tgggttctc ccattggcc tttcatgtc agtgcattc ctggggaga gactccttt cggaacctg gcagttgga aatacataa cgacggacg ccttcttgc gaacgtgac tgcctggga tttgactct gtatggcac ggccacacc gccggccgc cgacctgaa gcaggagac ggtgcacca gccctccat tgtggacat ataccccga agagatcga tgataattc gattcaaga cgcgtcggc caattccct caagtttgg tgcagaggt taagaaaga tacgtccgg ttgcttaaa aactaatgg ccagttatg tttagacac tctctatgt cctattaat atatactat cagttcact aattttcat tttcgactt gtatttaaa cattcggat caagtcttg ccacattaa acgtagcat cattgagtt tcataatgt aaacagatc atacaatct ttactgtat tcattcaac cacaaatca aaacaaaga aaattctga caacaattt tcacagaga cacacggaa aaacaaatg taaagtcac gcgacatat atttaaaga cgttttttt gaatcgtac gtttagaga ctttttagg gaaggtagg gaatttaaa ggttttttt gcgaataat gtatcataa cgttcgcga atggttaca tagactaaa tacgcttag attagtagt actattttg tgatatctc aacgtttta tatattttt taagctaaa gtgttcaat attatgtat gttaccgtc ttattcgga agtatccaa ctgaatttc tttcaaccg taatatatt tacaccact cttatcata ttatgtcag ttttgttat gtttatgaa atatttatt tttatctat tgtacagtt ttattgttt tattaacaa ttctatcta ttgtccagt gaaatacgc tagctcgat cactttgta cttagcaaa gttacttca cctgttttg gtccacgca gcaaggttg acacttttg tacttttca catttttta atgttcgcc tgtctgaaa acataccag tatttcgcg agtaatatt aagatgtat gtgccaaat ttaagagta agtaggacg tagatgtta caaaaagga caaggtaat cgcagaacc gttcgcata ttcgacata taagtgcaa ttacctaac aacctacaa aagtaccaa tacttttag acaaacgga aatacaata aaggtggta tagatcgat atgttttac gttcggatt atttttcta ctttacacg caatggtaa tgttagctg tgattattt aattattgt acatacttg aaagactca tttatattt tattatgtt gttaatttt ttaatagta gttcctgaa acaagttca ccattaatg ttaaggtac ctatgtaca ttgagaatt tgtctgata cttaaaatt gtaagtaaa ttacttaat atgccttaa ttttgtcac gttgatttg acgaatact cgaccgagt atcagctca atgaatgaa tgggtgttt gtgtgtagt aaatgtctg tacttatct gtattttta actttagtc ttagatatt ctgaaaatt ctttagtcc tcaaggcct agtctttag ccattctgt tgagatttt atttatttt taccaaaga cccctcttt ggccgactt ttttatgca tcattctca acggacttg ctaataact aaaatatat tgttataat acatgtaca tcacctgtt tgtaaacta taaaagaat aataaattg tggctaaaa cgagtcgtt caaaattat ttcaacatc cagcatctg ccacgggac cgatcgctt ccttaggta agtccccaa agtcaacta atttgcatg ttttgtatt gtgttaaaa ttgttgtca gattttttt tattttgct ttcctgtag ataacgcta attcttttc catacctta cctgcctca gcaccatta acagtgcca taattttag gctgtccgg aaaatttat ttttacaca agggtcgat gtatcaaaa gttgtcctg acggctttt accatttga cgcttaggc gcgagaggc cttcggagg ccgaagatt tatctccgg aaaataaca ccaatcggt gcttaccat gccattaca cttgtaaga agtgcacag gcccggtgg actaccagt gttccgagt gtttcccag atgcccaac tggtgctgg tttccagca ccgaaactt tttgttact ccgcatctg tggcctctt caatagtag cagataaat ggtatataa gggtatttg ttatgttac ttatagtta gcgttttat tagtattgc atttattgt ggcaaattt ttaaatcaa ggaaacggc cccgcggag ggtacttgc gttttaatc tgataaaca atagtgatg aaaaagatg taaaaatgg ctgtttata gtgatagcg aataatatt ttaaacttt gatgtgact ttactccta ctgtaaata gaagaattg ttatttttt gagtattaa agccaataa agtattata aagctt
- >TC010869 RB
cggctccga aaacatgaa aacaaccgc agcattgtg agcacaatg ttataagga cctcgatac tagaaaatt cagcaaagc gatgaaggg gaaaggtat tggggaaaa caacttgat caacgttat taaagatag caaacgcat ctcaatttt caacttgga aaatcctac gattttcga ggactacta cctcttcac ccacagcgg ggtctccaa acgatccct cagcttgaa ttccgaaaa tcgggcaaa actagcagt cgacaccag ggtcaggtg gtcaaaaca acaaacggc aaagaaacg agttaaaag agacctaag actgcaaga ctcagaccc caaatggcc cagcatgtg gtatctgaa ccgaggaaa tggtctaga tatgaacgt tattcccgc ctggctaga gggcataac cggcaaagg cgccgtcgt aaccattct agacgatgg tctcgagaa ggaccatcc agatctagt acaaaatta cgacccctt ggcctcata cgacgtcaa cagtcacga ttctgatcc tagtccaag atacgatat gatagactc aaaccggca cggcactcg ctgtgctgg tgaagtggc tgcgactag taataactc ggtttgtgc tctgggtgt tgcccacgg tgcccaagt gggcggcgt gcggatgct ggatgggga tgtaaccga cgcggtgga ggctcgctc cctgagcct caacccgca gcacatcga catctacag cgcgtcttg gggccccga cgacgacgg caaaaccgt cgacgggcc cggggagct cgccacgcg ggcgttcat cgaaggggt gacaaaggg ccgtaatgg gaaaggttc gatttttgt gtgggcgtc ggggaacgg cggccggga tcacgataa ttgcaactg tgacgggta cacgaattc gatctatag tttgtcgat atcgagtgc gacggaaca cggacacgt tccgtggta cagtgaggc gtgcagttc cacccttgc ctcgactta cagtagcgg ggctgtggg ggaacgcca ggtggtgac cactgattt gaggtattc ttgcactag tagccacac ggggaccag tgcctcggc gccgcttgc agcgggcat ctgtgcact ggctctgga ggccaatcc caacctgac ctggaggga catgcaaca cattgttgt tcggaccgc caggcccga aaatctgat cgcccccga ctggcagac caacggcgt ggggcgaaa tgtctcaca tagttttgg ctatgggct tatggacgc ctacgccat ggtacaact ctcgaggaa ttggatcac agtgcctga acaacacaa atgcgaaat cactgcgcc tcacgtcca aaggcctat tcctgccaa aagcgtagt cgttttaca gttacaagt aaaggagtg tgagggagt tgaagtctt agaacacgt ccaagctaa attaactat ctattcgca aagacgtgg cgacctgaa tattcagtt gacctcccc tatgggaac gcgggtaac actattagc ccatagggc ccacgacac ttccagggt tgggttctc ccattggcc tttcatgtc agtgcattc ctggggaga gactccttt cggaacctg gcagttgga aatacataa cgacggacg ccttcttgg tcgagcgac acttcagaa ctggtcttt ggtcctgta tgggaccaa gcagtttct ccctccttt atctaataa taatataga aactaacaa agtcaacaa aactaaaac aaataatat atttaaaaa gaaaaactc gaaaaagca aaaaaacaa aaacttgca gaaagtcac gacaactaa accccccaa atcccaata caaactaaa gttaaagaa cacaacgcc gaaaatcag cgttcctaa cgtaacaaa ttcgaaaaa tcgcaaaat agccaagac cacgaccga gttctcgcc ctactttta cgcgataaa caccagtaa atacaaaaa ttcggagga cagtgttaa gcagtactt gaaatttgt taagaacat tacgataac ttacccggt catgattcc ctcccgcga gatgtcgaa acctcgcga atcgttcaa aaaatcgca aaaacagaa agagaaaag ccgcgatat taactcgaa ccgcaacgc aaaagtttt cacttctaa aacgactga attaacaac ctatcaaac taccaccag ttccccccg aggaagatt ggcgaacgt gactgcctg ggatttgac tctgtatgg cacggccac accgccggc cgccgacct gaagcagga gacggtgca ccagccctc cattgtgga catataccc cgaagagat cgatgataa ttcgattca agacgcgtc ggccaattc cctcaagtt tggtgcaga ggttaagaa agatacgtc cggttgctt aaaaactaa tggccagtt atgtttagg ctgtccgga aaatttatt tttacacaa gggtcgatg tatcaaaag ttgtcctga cggctttta ccatttgac gcttaggcg cgagaggcc ttcggaggc cgaagattt atctccgga aaataacac caatcggtg cttaccatg ccattacac ttgtaagaa gtgcacagg cccggtgga ctaccagtg ttccgagtg tttcccaga tgcccaact ggtgctggt ttccagcac cgaaacttt ttgttactc cgcatctgt ggcctcttc aatagtagc agataaatg gtatataag ggtatttgt tatgttact tatagttag cgttttatt agtattgca tttattgtg gcaaatttt taaatcaag gaaacggcc ccgcggagg gtacttgcg ttttaatct gataaacaa tagtgatga aaaagatgt aaaaatggc tgtttatag tgatagcga ataatattt taaactttg atgtgactt tactcctac tgtaaatag aagaattgt tattttttg agtattaaa gccaataaa gtattataa agctt
- >TC010869 RC
cggctccga aaacatgaa aacaaccgc agcattgtg agcacaatg ttataagga cctcgatac tagaaaatt cagcaaagc gatgaaggg gaaaggtat tggggaaaa caacttgat caacgttat taaagatag caaacgcat ctcaatttt caacttgga aaatcctac gattttcga ggactacta cctcttcac ccacagcgg ggtctccaa acgatccct cagcttgaa ttccgaaaa tcgggcaaa actagcagt cgacaccag ggtcaggtg gtcaaaaca acaaacggc aaagaaacg agttaaaag agacctaag actgcaaga ctcagaccc caaatggcc cagcatgtg gtatctgaa ccgaggaaa tggtctaga tatgaacgt tattcccgc ctggctaga gggcataac cggcaaagg cgccgtcgt aaccattct agacgatgg tctcgagaa ggaccatcc agatctagt acaaaatta cgacccctt ggcctcata cgacgtcaa cagtcacga ttctgatcc tagtccaag atacgatat gatagactc aaaccggca cggcactcg ctgtgctgg tgaagtggc tgcgactag taataactc ggtttgtgc tctgggtgt tgcccacgg tgcccaagt gggcggcgt gcggatgct ggatgggga tgtaaccga cgcggtgga ggctcgctc cctgagcct caacccgca gcacatcga catctacag cgcgtcttg gggccccga cgacgacgg caaaaccgt cgacgggcc cggggagct cgccacgcg ggcgttcat cgaaggggt gacaaaggg ccgtaatgg gaaaggttc gatttttgt gtgggcgtc ggggaacgg cggccggga tcacgataa ttgcaactg tgacgggta cacgaattc gatctatag tttgtcgat atcgagtgc gacggaaca cggacacgt tccgtggta cagtgaggc gtgcagttc cacccttgc ctcgactta cagtagcgg ggctgtggg ggaacgcca ggtggtgac cactgattt gaggtattc ttgcactag tagccacac ggggaccag tgcctcggc gccgcttgc agcgggcat ctgtgcact ggctctgga ggccaatcc caacctgac ctggaggga catgcaaca cattgttgt tcggaccgc caggcccga aaatctgat cgcccccga ctggcagac caacggcgt ggggcgaaa tgtctcaca tagttttgg ctatgggct tatggacgc ctacgccat ggtacaact ctcgaggaa ttggatcac agtgcctga acaacacaa atgcgaaat cactgcgcc tcacgtcca aaggcctat tcctgccaa aagcgtagt cgttttaca gttacaagt aaaggagtg tgagggagt tgaagtctt agaacacgt ccaagctaa attaactat ctattcgca aagacgtgg cgacctgaa tattcagtt gacctcccc tatgggaac gcgggtaac actattagc ccatagggc ccacgacac ttccagggt tgggttctc ccattggcc tttcatgtc agtgcattc ctggggaga gactccttt cggaacctg gcagttgga aatacataa cgacggacg ccttcttgg tcgagcgac acttcagaa ctggtcttt ggtcctgta tgggaccaa gcagtttct ccctccttt atctaataa taatataga aactaacaa agtcaacaa aactaaaac aaataatat atttaaaaa gaaaaactc gaaaaagca aaaaaacaa aaacttgca gaaagtcac gacaactaa accccccaa atcccaata caaactaaa gttaaagaa cacaacgcc gaaaatcag cgttcctaa cgtaacaaa ttcgaaaaa tcgcaaaat agccaagac cacgaccga gttctcgcc ctactttta cgcgataaa caccagtaa atacaaaaa ttcggagga cagtgttaa gcagtactt gaaatttgt taagaacat tacgataac ttacccggt catgattcc ctcccgcga gatgtcgaa acctcgcga atcgttcaa aaaatcgca aaaacagaa agagaaaag ccgcgatat taactcgaa ccgcaacgc aaaagtttt cacttctaa aacgactga attaacaac ctatcaaac taccaccag ttccccccg aggaagatt ggcgaacgt gactgcctg ggatttgac tctgtatgg cacggccac accgccggc cgccgacct gaagcagga gacggtgca ccagccctc cattgtgga catataccc cgaagagat cgatgataa ttcgattca agacgcgtc ggccaattc cctcaagtt tggtgcaga ggttaagaa agatacgtc cggttgctt aaaaactaa tggccagtt atgtttaga cactctcta tgtcctatt aatatatac tatcagttc actaatttt cattttcga cttgtattt aaacattcg gatcaagtc ttgccacat taaacgtag catcattga gtttcataa tgtaaacag atcatacaa tctttactg tattcattc aaccacaaa tcaaaacaa agaaaattc tgacaacaa ttttcacag agacacacg gaaaaacaa atgtaaagt cacgcgaca tatatttaa agacgtttt tttgaatcg tacgtttag agacttttt agggaaggt agggaattt aaaggtttt tttgcgaat aatgtatca taacgttcg cgaatggtt acatagact aaatacgct tagattagt agtactatt ttgtgatat ctcaacgtt ttatatatt ttttaagct aaagtgttc aatattatg tatgttacc gtcttattc ggaagtatc caactgaat ttctttcaa ccgtaatat atttacacc actcttatc atattatgt cagttttgt tatgtttat gaaatattt atttttatc tattgtaca gttttattg ttttattaa caattctat ctattgtcc agtgaaata cgctagctc gatcacttt gtacttagc aaagttact tcacctgtt ttggtccac gcagcaagg ttgacactt ttgtacttt tcacatttt ttaatgttc gcctgtctg aaaacatac cagtatttc gcgagtaat attaagatg tatgtgcca aatttaaga gtaagtagg acgtagatg ttacaaaaa ggacaaggt aatcgcaga accgttcgc atattcgac atataagtg caattacct aacaaccta caaaagtac caatacttt tagacaaac ggaaataca ataaaggtg gtatagatc gatatgttt tacgttcgg attattttt ctactttac acgcaatgg taatgttag ctgtgatta tttaattat tgtacatac ttgaaagac tcatttata ttttattat gttgttaat tttttaata gtagttcct gaaacaagt tcaccatta atgttaagg tacctatgt acattgaga atttgtctg atacttaaa attgtaagt aaattactt aatatgcct taattttgt cacgttgat ttgacgaat actcgaccg agtatcagc tcaatgaat gaatgggtg tttgtgtgt agtaaatgt ctgtactta tctgtattt ttaacttta gtcttagat attctgaaa attctttag tcctcaagg cctagtctt tagccattc tgttgagat tttatttat ttttaccaa agacccctc tttggccga cttttttat gcatcattc tcaacggac ttgctaata actaaaata tattgttat aatacatgt acatcacct gtttgtaaa ctataaaag aataataaa ttgtggcta aaacgagtc gttcaaaat tatttcaac atccagcat ctgccacgg gaccgatcg cttccttag gtaagtccc caaagtcaa ctaatttgc atgttttgt attgtgtta aaattgttg tcagatttt ttttatttt gctttcctg tagataacg ctaattctt ttccatacc ttacctgcc tcagcacca ttaacagtg ccataattt taggctgtc cggaaaatt tatttttac acaagggtc gatgtatca aaagttgtc ctgacggct tttaccatt tgacgctta ggcgcgaga ggccttcgg aggccgaag atttatctc cggaaaata acaccaatc ggtgcttac catgccatt acacttgta agaagtgca caggcccgg tggactacc agtgttccg agtgtttcc cagatgccc aactggtgc tggtttcca gcaccgaaa ctttttgtt actccgcat ctgtggcct cttcaatag tagcagata aatggtata taagggtat ttgttatgt tacttatag ttagcgttt tattagtat tgcatttat tgtggcaaa tttttaaat caaggaaac ggccccgcg gagggtact tgcgtttta atctgataa acaatagtg atgaaaaag atgtaaaaa tggctgttt atagtgata gcgaataat attttaaac tttgatgtg actttactc ctactgtaa atagaagaa ttgttattt tttgagtat taaagccaa taaagtatt ataaagctt
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