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Gene information
General information
TC010858 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5401420..5407558
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010858
    iBeetle screen iB_01750

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    remarks: later than schedule, adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: bad quality of preparation,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010858 RA
      MNTEAGVPD GGWGWVVVA GTAIINIVN QSLLSMFGL 
      IFGPKLEMM AGTTGVTTV MSLNCTISN FSGFLISPL 
      SKYFSTKQI TQIGVLLVS AGLAFSTYT KSYMRITIG 
      YGVLTGLGL GLIASSTFL IINSYFDKN KSQAVGLSM 
      AGTSIGQMG MPPIVALLI QHYGYDGTI LILSGLSLH 
      GLIGSLFFD PVSEHMLPR SRQKTKGFF SRFRDRISC 
      RRNRKRVVI ENNLSPDRT DPELTNKCA NNNIAREAN 
      LTKLEAGNK ESVIHNECT IKNEKIADI INESREGES 
      VLSNNLQTE TADKFEFSA SDNENNEPD EEKRMLPPE 
      TAATQKEEK IDKDEPQTC YQKLVELFD LYILLDPIF 
      VHIMVGLSF AYTTSISFS TFFPLFLRE DLDMTIIDV 
      STCMSVLSF MDFIGRVVV PLITKRMEI THRMIFIIG 
      SFLLAICRS VLAMQTTYS MLILLSAVC GLARAATVI 
      NQNLVIAEY SKEAHISSA VGLNMVCKG IFVLTIGRL 
      LGTYKDSAH NFSSCIHIL NIISGGVVV SWTLELIIL 
      KLCKKKNAT
    CDS sequence
    • >TC010858 RA
      atgaacact gaagctggg gtccccgat ggaggatgg 
      gggtgggta gttgttgct ggaactgcg ataattaat 
      atagttaat cagtcacta ttgtctatg tttggttta 
      atatttggt ccgaagttg gaaatgatg gctggaacg 
      actggagtt acaactgtt atgagtctg aactgcaca 
      atcagtaac ttttctgga tttctaata agccccctt 
      tctaaatat ttcagtacg aaacagata actcaaatt 
      ggggtattg ctggtctca gcaggattg gcgttctca 
      acgtatacg aaaagttac atgagaatt acaatagga 
      tatggtgtt ctaacaggt ctgggattg ggattaata 
      gcctcttcg acgtttctt attatcaat tcttatttt 
      gataaaaat aaaagtcag gctgttggc ctgtctatg 
      gcgggaaca agtataggt caaatggga atgccgcca 
      atagtggct ctccttatt caacattac ggttatgat 
      ggcacaata ttaatttta tcgggactt agcttacac 
      ggtttgatc ggatcgttg ttttttgat cctgttagc 
      gaacacatg ttgccaagg agtagacag aaaacaaag 
      ggcttcttc tcacgattt cgcgacagg attagctgt 
      cgcagaaac cgaaagaga gtcgttata gaaaataat 
      ctgtctccc gataggact gatcccgaa ctaacaaat 
      aagtgtgct aataataac attgctaga gaagctaat 
      ttaactaaa cttgaagca ggaaacaag gaatctgtg 
      atacataat gaatgcaca atcaaaaat gaaaagatc 
      gctgatatt atcaatgaa agtcgcgaa ggtgaaagt 
      gtgctgtct aataattta caaactgag actgctgac 
      aaattcgaa ttttctgca tctgacaac gaaaacaac 
      gaaccagac gaagagaaa aggatgttg cctccggaa 
      acggccgcc acacaaaaa gaagagaaa atcgataag 
      gatgaaccg caaacttgc taccaaaaa ctcgtcgaa 
      cttttcgat ctttacatt ttgctagat ccgattttt 
      gtccacatt atggtcggc ctttccttc gcctataca 
      acgagtata tcattcagt acatttttc cctttgttt 
      ttgagagaa gatctcgac atgactatc atcgacgtg 
      tccacttgt atgtcagtg ctatcattt atggatttt 
      ataggacga gttgtcgtc cctctaatt acaaaacga 
      atggaaatc acccatcgg atgatcttt ataatagga 
      tcattcctt ttggctatt tgtagatct gtattggca 
      atgcaaact acatacagt atgctaatt ttgctctct 
      gctgtttgt ggattagct agagctgct acagttatt 
      aaccaaaat cttgtcatt gcggaatac tctaaagag 
      gctcacatt tcatcagct gtgggcctg aatatggtg 
      tgcaaagga atctttgtt ttaactatt ggtagattg 
      ttgggtact tacaaggat tcggctcac aacttcagt 
      agctgtatt catattttg aatataatt tctggtgga 
      gtagtggtt tcttggaca ctcgaactt attatctta 
      aagttgtgt aaaaagaaa aatgctacg tga
    mRNA sequence
    • >TC010858 RA
      tggaaagca aattttgcg ttaattgac gcttctgtc 
      tttgatttg aattccagt cgataaaat ttcgcagct 
      aaggtggct tatgctaaa gaaatcacc gactggtcc 
      ctggacggt gcaggtctt acaaaactt tcctcgaga 
      gcagaattt aaatgaaat gtttccact atttagtgt 
      gtgccgcga tttcaacag gactctgta gatttaatt 
      cttctttaa gttgggcaa cgttatcgc acgaaatgc 
      agacgctgt ggctaaaac aggatgcat aattttcta 
      aatataata acacgcgaa tttcctgat tctgtttac 
      catttacgg agttaaaag tgaggatag gtttggtaa 
      agtgtgatt ttggtgttg tgtctcata atgttaggg 
      atgtttaac gttaatttc ctcgaagca ttagtcttg 
      ggtaagtaa gtagttcca aagttgggg aataaccca 
      attagaagt tgtctttga taaatccat tcacaagag 
      tagaaaatt ttaattgga acaaagcta tgagaatag 
      tgtgaacaa taatactcg cttgtttgc tgaataaga 
      taatattcg aatcgtgta tttaagaag gctgaatag 
      cgaacagtt ttttacatt aaaacagaa cgaatagtc 
      agtcagtag ttcaatata ttattgtct agagcatga 
      atattcatg gagttctcc gtcctttca gttagtacc 
      taagcaagc gtattaaaa ttgtgaatt aggtgaatt 
      tcgttaagc atttcctcg agaccaaat aataaaaca 
      gcaaaaacg tgccctatc gagactgcc gatgtaagt 
      atgtaattg tattagtac atgtattaa gttccacat 
      ttctgcatt atctgactt gataaaatc ctaatcgga 
      atacagcta catatcaat aaacccttc acacttcga 
      taacactat aataacaac tttatatcg tctagtgtg 
      tgataatgt tcgtgggca aaaggatgt gaatttttt 
      tatcacttt tctcgcaac atatgtacg gttacctac 
      ttacgaatc gagaaactt gattgagaa tcctgtaca 
      agaggaaaa tttcactaa ctgttacct gtaaccgga 
      ggagcgcgt gtttagaat tcttttcga atgtttctg 
      gttgttatt acagtctac tcttggact aatcaataa 
      ttttggtgt tgcttctat tccactcta gatggcgaa 
      ccgccagca aaatgtgaa aatcgctcc tcaaaacgg 
      caaatttca aatttcact ttgcgattt caatggaat 
      tgttcgtcc gattaatta aataagtta ttattttat 
      tattattaa tcgtggtct gcatctttg gcatcgtca 
      taattcaaa ggcaaatta taaatcata ttgtcggct 
      cttatagtg agaaataat gcagaaaca atgttactg 
      gggatagtg aattgacag atcattaat aactctagg 
      gccataact ttaacatac atgctaggg tggagtttt 
      tcttataac gctgcattg tttcgaaat taaacacat 
      tcgaggaaa gaatgaaaa gctgataat tgtaaaatt 
      ttactgatt aggaatata cctttctca ttattttta 
      cgatagtta tgagataag tgcggagtt accctgtcc 
      cgaactcac tgtcacgat gaacactga agctggggt 
      ccccgatgg aggatgggg gtgggtagt tgttgctgg 
      aactgcgat aattaatat agttaatca gtcactatt 
      gtctatgtt tggtttaat atttggtcc gaagttgga 
      aatgatggc tggaacgac tggagttac aactgttat 
      gagtctgaa ctgcacaat cagtaactt ttctggatt 
      tctaataag ccccctttc taaatattt cagtacgaa 
      acagataac tcaaattgg ggtattgct ggtctcagc 
      aggattggc gttctcaac gtatacgaa aagttacat 
      gagaattac aataggata tggtgttct aacaggtct 
      gggattggg attaatagc ctcttcgac gtttcttat 
      tatcaattc ttattttga taaaaataa aagtcaggc 
      tgttggcct gtctatggc gggaacaag tataggtca 
      aatgggaat gccgccaat agtggctct ccttattca 
      acattacgg ttatgatgg cacaatatt aattttatc 
      gggacttag cttacacgg tttgatcgg atcgttgtt 
      ttttgatcc tgttagcga acacatgtt gccaaggag 
      tagacagaa aacaaaggg cttcttctc acgatttcg 
      cgacaggat tagctgtcg cagaaaccg aaagagagt 
      cgttataga aaataatct gtctcccga taggactga 
      tcccgaact aacaaataa gtgtgctaa taataacat 
      tgctagaga agctaattt aactaaact tgaagcagg 
      aaacaagga atctgtgat acataatga atgcacaat 
      caaaaatga aaagatcgc tgatattat caatgaaag 
      tcgcgaagg tgaaagtgt gctgtctaa taatttaca 
      aactgagac tgctgacaa attcgaatt ttctgcatc 
      tgacaacga aaacaacga accagacga agagaaaag 
      gatgttgcc tccggaaac ggccgccac acaaaaaga 
      agagaaaat cgataagga tgaaccgca aacttgcta 
      ccaaaaact cgtcgaact tttcgatct ttacatttt 
      gctagatcc gatttttgt ccacattat ggtcggcct 
      ttccttcgc ctatacaac gagtatatc attcagtac 
      atttttccc tttgttttt gagagaaga tctcgacat 
      gactatcat cgacgtgtc cacttgtat gtcagtgct 
      atcatttat ggattttat aggacgagt tgtcgtccc 
      tctaattac aaaacgaat ggaaatcac ccatcggat 
      gatctttat aataggatc attcctttt ggctatttg 
      tagatctgt attggcaat gcaaactac atacagtat 
      gctaatttt gctctctgc tgtttgtgg attagctag 
      agctgctac agttattaa ccaaaatct tgtcattgc 
      ggaatactc taaagaggc tcacatttc atcagctgt 
      gggcctgaa tatggtgtg caaaggaat ctttgtttt 
      aactattgg tagattgtt gggtactta caaggattc 
      ggctcacaa cttcagtag ctgtattca tattttgaa 
      tataatttc tggtggagt agtggtttc ttggacact 
      cgaacttat tatcttaaa gttgtgtaa aaagaaaaa 
      tgctacgtg atagtatga gttcgttta gttccattc 
      agtattgtg gtatatgca tagtactta aagcattgt 
      ttgaatctg tacatgttg ttattaaag atttattta 
      gaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY