Status
Please wait ...
Gene information
General information
TC010855 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5516839..5520095
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010855
    iBeetle screen iB_01749

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010855 RA
      MGLNGSSGS GTQPINRRK NLSQQIWDV VQLIGDVVL 
      LWVLVLYSL GERFYRYIV PVPERPVKG EIVLITGTG 
      HGMGKELAL LYASKGATV VGWDVNTKS NEETISEIN 
      ARGYPKAYA YYCDVSNKD SVFEVAKKV LKEVGDVSI 
      LINNAGIMP THPILDQTK EEIEKTFAI NVFAHFWTI 
      QAFLPTMKK NNHGHIVAL SSCAGLFGL ENLVPYCGT 
      KFAVRGIME ALHEEFRQE KNCKIETTC VCPYMVDTG 
      LCKKPKVRF ERLMPLLNP RYAAKSIMK AQQTQQKLV 
      SIPGYLVNL NDFVRVLPL KAGLYIKDF INSGVESDL
    • >TC010855 RB
      MVEVHAKSK MGLNGSSGS GTQPINRRK NLSQQIWDV 
      VQLIGDVVL LWVLVLYSL GERFYRYIV PVPERPVKG 
      EIVLITGTG HGMGKELAL LYASKGATV VGWDVNTKS 
      NEETISEIN ARGYPKAYA YYCDVSNKD SVFEVAKKV 
      LKEVGDVSI LINNAGIMP THPILDQTK EEIEKTFAI 
      NVFAHFWTI QAFLPTMKK NNHGHIVAL SSCAGLFGL 
      ENLVPYCGT KFAVRGIME ALHEEFRQE KNCKIETTC 
      VCPYMVDTG LCKKPKVRF ERLMPLLNP RYAAKSIMK 
      AQQTQQKLV SIPGYLVNL NDFVRVLPL KAGLYIKDF 
      INSGVESDL
    CDS sequence
    • >TC010855 RA
      atgggttta aatggaagc agtggctct gggacacag 
      cccataaac cggaggaaa aatttgagc cagcaaatc 
      tgggatgta gtgcaacta attggggac gtagtgtta 
      ctgtgggtg ctagttttg tacagtttg ggggagcgt 
      ttctatagg tatatagtg ccagtgccg gaacggccg 
      gttaaaggg gaaattgtt ttgataacc ggcaccggc 
      catgggatg ggcaaagag ctggcgctt ttgtacgcc 
      tcaaagggc gctactgtg gtgggctgg gacgtaaac 
      acaaaaagc aatgaagag actatttcg gagataaat 
      gcccgcggt taccccaaa gcttacgcc tattactgc 
      gacgtctca aacaaagac agcgttttc gaagtcgcc 
      aaaaaagta ctaaaagag gtcggggat gtttcaatt 
      ttgatcaac aacgctgga ataatgccc acgcatccg 
      attctcgac caaacgaaa gaagagatt gaaaaaacc 
      ttcgcgatc aatgttttc gctcatttt tggacaatt 
      caagctttt cttcccact atgaagaaa aataaccac 
      ggtcacatt gtagctctg tcttcctgt gccggtttg 
      ttcggcctt gagaacttg gttccttac tgcggtacc 
      aagtttgcc gttcgtggc attatggag gctttacat 
      gaggaattt cggcaagaa aaaaattgc aaaattgag 
      acaacttgt gtatgccct tacatggtc gacaccggc 
      ttgtgcaaa aagccgaag gttaggttt gagcggctt 
      atgccgctt ctgaacccc agatatgct gcaaaatcc 
      atcatgaaa gcgcagcag acgcaacag aaattggtg 
      tcgataccc ggctatctg gtcaacttg aacgatttt 
      gttagggta ctaccatta aaggccggt ttgtacatc 
      aaagacttc atcaactct ggtgtagag tcagactta 
      taa
    • >TC010855 RB
      atggtggag gtccacgcc aaatcaaaa atgggttta 
      aatggaagc agtggctct gggacacag cccataaac 
      cggaggaaa aatttgagc cagcaaatc tgggatgta 
      gtgcaacta attggggac gtagtgtta ctgtgggtg 
      ctagttttg tacagtttg ggggagcgt ttctatagg 
      tatatagtg ccagtgccg gaacggccg gttaaaggg 
      gaaattgtt ttgataacc ggcaccggc catgggatg 
      ggcaaagag ctggcgctt ttgtacgcc tcaaagggc 
      gctactgtg gtgggctgg gacgtaaac acaaaaagc 
      aatgaagag actatttcg gagataaat gcccgcggt 
      taccccaaa gcttacgcc tattactgc gacgtctca 
      aacaaagac agcgttttc gaagtcgcc aaaaaagta 
      ctaaaagag gtcggggat gtttcaatt ttgatcaac 
      aacgctgga ataatgccc acgcatccg attctcgac 
      caaacgaaa gaagagatt gaaaaaacc ttcgcgatc 
      aatgttttc gctcatttt tggacaatt caagctttt 
      cttcccact atgaagaaa aataaccac ggtcacatt 
      gtagctctg tcttcctgt gccggtttg ttcggcctt 
      gagaacttg gttccttac tgcggtacc aagtttgcc 
      gttcgtggc attatggag gctttacat gaggaattt 
      cggcaagaa aaaaattgc aaaattgag acaacttgt 
      gtatgccct tacatggtc gacaccggc ttgtgcaaa 
      aagccgaag gttaggttt gagcggctt atgccgctt 
      ctgaacccc agatatgct gcaaaatcc atcatgaaa 
      gcgcagcag acgcaacag aaattggtg tcgataccc 
      ggctatctg gtcaacttg aacgatttt gttagggta 
      ctaccatta aaggccggt ttgtacatc aaagacttc 
      atcaactct ggtgtagag tcagactta taa
    mRNA sequence
    • >TC010855 RA
      tacaatttg agaaagcgt tcaagagtg acttcaggt 
      gtcgtgcga acaactcat ataagcggg ggagtgggt 
      gtccataaa cccaaaaaa ggagagaat cttggaagg 
      aatttgaga taaattgcg agcggcacg cacgaataa 
      ataatcgct gataagcag tgggtgaca attggacgt 
      accatctat ttattttgt tgcatgata agaggacga 
      aaactgtcc gaacgtttg gttcttttc gctagcatt 
      agtgcataa tttttatag cgcttttat gacttcatt 
      aatcgcgtt catatataa aatatgaaa ttataaacc 
      aacacacac gctgctgag tagactagc cagcaggtt 
      tcagttggt gttttaacc aacagacgt tgaacgtgt 
      tccctgaag cgcgagaca tggtggagg tccacgcca 
      aatcaaaaa tgggtttaa atggaagca gtggctctg 
      ggacacagc ccataaacc ggaggaaaa atttgagcc 
      agcaaatct gggatgtag tgcaactaa ttggggacg 
      tagtgttac tgtgggtgc tagttttgt acagtttgg 
      gggagcgtt tctataggt atatagtgc cagtgccgg 
      aacggccgg ttaaagggg aaattgttt tgataaccg 
      gcaccggcc atgggatgg gcaaagagc tggcgcttt 
      tgtacgcct caaagggcg ctactgtgg tgggctggg 
      acgtaaaca caaaaagca atgaagaga ctatttcgg 
      agataaatg cccgcggtt accccaaag cttacgcct 
      attactgcg acgtctcaa acaaagaca gcgttttcg 
      aagtcgcca aaaaagtac taaaagagg tcggggatg 
      tttcaattt tgatcaaca acgctggaa taatgccca 
      cgcatccga ttctcgacc aaacgaaag aagagattg 
      aaaaaacct tcgcgatca atgttttcg ctcattttt 
      ggacaattc aagcttttc ttcccacta tgaagaaaa 
      ataaccacg gtcacattg tagctctgt cttcctgtg 
      ccggtttgt tcggccttg agaacttgg ttccttact 
      gcggtacca agtttgccg ttcgtggca ttatggagg 
      ctttacatg aggaatttc ggcaagaaa aaaattgca 
      aaattgaga caacttgtg tatgccctt acatggtcg 
      acaccggct tgtgcaaaa agccgaagg ttaggtttg 
      agcggctta tgccgcttc tgaacccca gatatgctg 
      caaaatcca tcatgaaag cgcagcaga cgcaacaga 
      aattggtgt cgatacccg gctatctgg tcaacttga 
      acgattttg ttagggtac taccattaa aggccggtt 
      tgtacatca aagacttca tcaactctg gtgtagagt 
      cagacttat aattttttc ctgaggtgg aagaacaac 
      attttttag aaagtacta acatttggt atgacatat 
      gtagcgaat aaaattgtg ttatttatt ggtttttat 
      acgtttatt cgttttttc taagggctg aattaattg 
      ttgaagtac ataaactga tgccagttg tattgttaa 
      taaaatatt ttgcaatat
    • >TC010855 RB
      tacaatttg agaaagcgt tcaagagtg acttcaggt 
      gtcgtgcga acaactcat acgttgaac gtgttccct 
      gaagcgcga gacatggtg gaggtccac gccaaatca 
      aaaatgggt ttaaatgga agcagtggc tctgggaca 
      cagcccata aaccggagg aaaaatttg agccagcaa 
      atctgggat gtagtgcaa ctaattggg gacgtagtg 
      ttactgtgg gtgctagtt ttgtacagt ttgggggag 
      cgtttctat aggtatata gtgccagtg ccggaacgg 
      ccggttaaa ggggaaatt gttttgata accggcacc 
      ggccatggg atgggcaaa gagctggcg cttttgtac 
      gcctcaaag ggcgctact gtggtgggc tgggacgta 
      aacacaaaa agcaatgaa gagactatt tcggagata 
      aatgcccgc ggttacccc aaagcttac gcctattac 
      tgcgacgtc tcaaacaaa gacagcgtt ttcgaagtc 
      gccaaaaaa gtactaaaa gaggtcggg gatgtttca 
      attttgatc aacaacgct ggaataatg cccacgcat 
      ccgattctc gaccaaacg aaagaagag attgaaaaa 
      accttcgcg atcaatgtt ttcgctcat ttttggaca 
      attcaagct tttcttccc actatgaag aaaaataac 
      cacggtcac attgtagct ctgtcttcc tgtgccggt 
      ttgttcggc cttgagaac ttggttcct tactgcggt 
      accaagttt gccgttcgt ggcattatg gaggcttta 
      catgaggaa tttcggcaa gaaaaaaat tgcaaaatt 
      gagacaact tgtgtatgc ccttacatg gtcgacacc 
      ggcttgtgc aaaaagccg aaggttagg tttgagcgg 
      cttatgccg cttctgaac cccagatat gctgcaaaa 
      tccatcatg aaagcgcag cagacgcaa cagaaattg 
      gtgtcgata cccggctat ctggtcaac ttgaacgat 
      tttgttagg gtactacca ttaaaggcc ggtttgtac 
      atcaaagac ttcatcaac tctggtgta gagtcagac 
      ttataattt tttcctgag gtggaagaa caacatttt 
      ttagaaagt actaacatt tggtatgac atatgtagc 
      gaataaaat tgtgttatt tattggttt ttatacgtt 
      tattcgttt tttctaagg gctgaatta attgttgaa 
      gtacataaa ctgatgcca gttgtattg ttaataaaa 
      tattttgca atat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY