Status
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Gene information
General information
TC010852 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5536334..5544233
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010852
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01748

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    Pupal morphology
    elytra orientation irregular - Number of animals showing the phenotype: 10

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010852 RA
      MAQCKQTPQ EFIKNAFTP QIAVLCTPL AEGSCQKNN 
      LNFIELLQP FSKLSNDVH YKDPAGTVI TIRNLKLTF 
      LDVNSRPPQ TTLARKFLN SSVSELSDF RTENFTTSD 
      HTLNITLTT PWFEAWRDT FLRVQYPSD HEYTKHFLS 
      CVLVVSSAE INVIESIIQ LSQSLNQMQ NNAPGKLPK 
      WFSSNVLKY YVIIHDNVE GNIDVANEA YESVKGSYG 
      EANCFLLRM NSRPPGAAN TEHLPDPWS QFISCNIES 
      RDYNPTPES SPYTSRAIF DTSQDSDTK TNLNYHPLS 
      PDTEDLIMS TSATEKDKT VSNNSKLKS HGLCLSTED 
      VEQVKLFLH EFTKTCLLP YIEKQIHQL SDVISNKKG 
      VSRSLFSAT KRWFNPNKP GASSVAVNN LIYAPDSPE 
      LQVRRLGDL YFMFGHYLL AFQAYHLAK RDYNTDQAW 
      LYYAGALEM AALAAFMAN ESSRKTYDY MEESITTYM 
      NTCKMPQFA TRATLLSSE CLKSRGLFG EAAHQLIRM 
      TSEESDLRS ALFLEQAAY CFLHSKMVR KYAFHMVLA 
      GHRFSKAAQ RKHSLRSYK QAHQIYEYS GWDLAEDHI 
      HYTIGRQAH NLHSFDEAV KSFARLLNG DSKQSGQQQ 
      SVFLKEYLT ILGNKLLKE DDDGIPILA LPELDSDSL 
      KLLMEPTPP LTTPGKVPA MGVNFLERD GAEVAARWN 
      KLEEILVQE AFGSLPLTF KPMITLYSV HNLAKNVPT 
      AIVNEPIQV CVQLINSLQ IVLQLKDIY LIWCFKNDD 
      LVASNENNS NNVDNFVKT HVTKSILLQ SNCNQNIIL 
      ALTPLVTGV ITLKGICYT LTSSNTPTD NIFIKGKQL 
      FNFDKSGTK NVEIKVVPL APCLQVTFS EFSLEFLCD 
      EVQKVTIDF QNTGTLPLH KVYMATSAP EYLCNCETK 
      TKELPEFSA NCTPAMREK FIRDNHITS VPLPNDRLE 
      PGQSTTVAI FIKAPNVPG PCLVDLLIY YENANAGVV 
      PRYRLVRHK WNLSVQESI KVDVTVQES FNAKQVEEI 
      AVVLKASNL NKLHSSTLT EISLLNVGM LSKFWSFIE 
      NVVTPKYIN LHSQESAHI LLKARRLNE KKSVYSNIY 
      LNHERSMRQ HLSSAYIAF AKKTEHCQV NLFNEFESS 
      PEGKDGIVF VQWQALVND PQKRVVDGQ TQIRIKVHK 
      TSDDQSDVD EFAALSDPA TLLEVSLNK DKQSENRTQ 
      QTKVTCTLI YPAIVEHNF QKEKFCVVP VTLLLHSVV 
      TDADLSVIV NTLGATSGP PPPTHSDLF YPYASKNFS 
      WLGTGSAVR EVKPLATEP VKLSAVVSR PGTFDLGAR 
      IEIFCSSTD QVDAPELQT CQIQSALIV IDGDS
    CDS sequence
    • >TC010852 RA
      atggctcag tgcaagcag actccgcaa gaatttata 
      aaaaacgcc ttcacaccc caaattgct gttttgtgc 
      acccctctc gctgaaggt tcttgtcag aaaaataat 
      ttgaatttc attgagctg ctccaaccc ttttccaaa 
      ttgagcaat gacgtgcat tacaaagat ccggccggc 
      acggttata acaataagg aacctcaaa ttaacgttt 
      ttggacgtg aattcccga cctccccaa accacttta 
      gctaggaaa tttttaaac tcttcagtg agtgaattg 
      tcagacttc cggactgag aatttcacc accagcgat 
      cacaccttg aacattact ttaacaacg ccgtggttt 
      gaggcttgg agagatacg tttttacga gttcagtat 
      ccctctgat catgaatat acaaagcat tttttatcg 
      tgtgttttg gttgtttct tcagctgaa atcaatgtc 
      attgaaagt attattcag ctgtctcag agtctgaat 
      caaatgcag aataacgcc cctggaaag ttacccaag 
      tggtttagc tcaaatgtt ttaaaatac tatgtgatc 
      atccatgac aatgttgaa ggaaatatt gatgtcgca 
      aatgaggcg tacgagtca gttaaaggg tcatacggg 
      gaggcgaat tgttttctt ttgaggatg aattcacgg 
      cccccaggt gccgcaaac accgagcat ttgccggac 
      ccttggagc caattcatt agctgtaat attgagtcc 
      agagactac aatccaacc cctgagagc tcaccctac 
      acttcacgg gcgattttt gataccagt caagactct 
      gatacaaaa accaactta aattaccac ccactgagc 
      cctgacact gaggattta ataatgagc acttcggca 
      actgaaaaa gacaaaacc gtatcaaac aactccaaa 
      ttaaaatcg cacggtctc tgcctgtcg actgaagac 
      gtcgaacag gtgaaatta tttttacac gagtttacc 
      aaaacctgt ctgctgcct tacatcgag aaacaaatc 
      caccaacta agcgacgta atttcgaac aaaaaaggc 
      gtcagcagg tcattgttc agtgcgaca aaacgctgg 
      ttcaacccc aataaaccg ggcgccagt tcggttgca 
      gtgaataat cttatctac gcgcctgat tcacccgaa 
      ttgcaagtg cggcgactt ggagatctt tatttcatg 
      tttggacat tatctacta gcgtttcaa gcatatcat 
      ttagctaaa agggactac aacacggat caggcctgg 
      ctgtactac gctggggcg ctcgaaatg gccgccttg 
      gcggctttt atggcgaat gagtcgagc agaaagacc 
      tacgattac atggaggag agcataacc acttacatg 
      aacacctgc aagatgcct cagtttgcg acccgagcg 
      acactttta agctccgag tgtctgaaa agtaggggt 
      ttgttcggc gaagcggcc catcaattg atacgaatg 
      acgagtgaa gagtcggat ttgagatcg gctcttttt 
      ctggaacag gccgcttat tgtttcctg cattcgaaa 
      atggtcaga aagtacgcc tttcatatg gttttggcc 
      ggccataga ttctcaaaa gctgctcaa aggaaacac 
      tcattgcgg agctacaaa caagcacac caaatttac 
      gaatacagt ggctgggat ttagctgag gatcacatt 
      cattataca atagggcgt caagctcac aatctccat 
      tcgtttgat gaggcggtt aagtcgttt gcgcggctg 
      cttaatggg gacagcaag cagtcgggt caacaacag 
      agcgtgttt ttgaaggaa tatttaaca atattaggg 
      aataaatta ctgaaagag gatgacgac ggtattccg 
      attttggct ctaccggag ttagacagt gattcttta 
      aagttgtta atggagccg acgcctccc ctaacaaca 
      cctgggaaa gttccggca atgggtgtc aattttctt 
      gaaagagat ggcgccgaa gttgcagcc aggtggaat 
      aaattggag gagatttta gtacaagaa gccttcggt 
      tctcttcct ctcacgttc aagccaatg atcacactt 
      tacagcgtc cataattta gcaaagaac gtccccaca 
      gcgattgta aatgagccg atccaagtc tgtgtgcaa 
      ctgattaat tcgttgcaa atcgtccta caattgaaa 
      gacatttat ttgatttgg tgcttcaaa aacgacgac 
      cttgtggct tctaatgaa aataacagc aataacgtt 
      gacaatttc gtcaaaacg cacgtcacg aagtcgatt 
      ttgctacaa agcaattgc aatcaaaat attatactt 
      gcgttaacg ccgcttgtg acaggggtg atcaccttg 
      aaagggatc tgctacacg ttaaccagt tccaacacg 
      cctacagac aatatattt atcaaagga aaacagttg 
      tttaatttc gacaaaagc ggcacgaag aacgtagaa 
      ataaaagtc gtgccgtta gcgccttgt ttgcaggtg 
      actttttcc gagttcagt ttggagttt ttatgcgac 
      gaagtacaa aaagttaca attgatttt caaaatacg 
      ggtacgctg cctttgcat aaggtgtac atggcgacg 
      tcggccccc gagtactta tgtaactgt gagacaaaa 
      accaaagaa ttgcccgaa ttttcggca aattgcact 
      ccagcaatg agggaaaaa tttatcagg gataaccac 
      attacttca gtacctctt ccaaacgac cgacttgag 
      ccggggcaa agcaccacg gtggccata tttatcaaa 
      gcgcctaat gttcccggg ccgtgtctt gtcgattta 
      cttatctat tacgaaaac gccaacgcg ggagtcgtc 
      ccaaggtat cggctcgtg cgccacaag tggaacttg 
      tcggtccag gagtccatc aaagtggac gtgacggtg 
      caagagagt ttcaatgcg aaacaagtg gaggagatc 
      gcagtggtg ctgaaagcg tcaaacttg aacaaactc 
      cacagctca acgctgacg gaaattagc cttttaaac 
      gtaggaatg ttgagtaaa ttctggagt ttcatcgaa 
      aacgtggta acccccaaa tacataaac ctacactct 
      caggaatcc gctcacatt ttactaaaa gcgcgccga 
      cttaacgaa aaaaagagc gtctactca aacatctac 
      ttgaaccat gagaggagc atgcggcaa cacctcagt 
      tccgcgtac atcgccttc gccaaaaag actgaacat 
      tgccaggtg aatttgttc aacgagttc gagtcttct 
      cctgaaggc aaggacggg atcgttttc gtgcaatgg 
      caagcgctg gtgaacgac ccgcagaag cgcgtagtc 
      gacggccaa acgcagatc cggataaaa gtccacaag 
      acgagcgat gatcaaagc gacgtcgac gagtttgcg 
      gctttgagc gacccggca actttgctc gaagtcagt 
      ttgaataaa gacaaacag agcgagaac cggacgcag 
      caaaccaaa gtcacatgc actctcatc tacccagcg 
      atcgtcgaa cacaatttc cagaaggag aaattctgt 
      gtggttccg gtcactctc ctcctccac agcgttgta 
      accgacgcc gatttgagt gtgatagtc aacactttg 
      ggcgccacg agtgggccg ccaccgccg acacactcc 
      gatcttttt tatccgtat gcttccaaa aatttttcc 
      tggcttggg acgggaagt gcagttaga gaagtcaag 
      cctttggcg acagaacca gtcaagctc tcggcagtt 
      gtttcgagg cccgggact tttgattta ggggcgcgt 
      attgagatt ttttgcagc agcaccgat caagtcgac 
      gctccggaa cttcaaacc tgccagatt caatcagca 
      ttaattgtt atagatggg gatagttaa
    mRNA sequence
    • >TC010852 RA
      gtgacccca aaaaatggt ttctttttt atcgtcacc 
      tgtcacacc tgtcaaaca gctgatttg cagctgatt 
      gtagtgttg ttaattttc cgattaacg caaaacagc 
      cttaaatga ttagaaatc gggtgtgtg actaaattt 
      aaaatcaac caaagtaca ttgtagagt taatcgatg 
      acttgacaa aatttttaa cctcacact catggctca 
      gtgcaagca gactccgca agaatttat aaaaaacgc 
      cttcacacc ccaaattgc tgttttgtg cacccctct 
      cgctgaagg ttcttgtca gaaaaataa tttgaattt 
      cattgagct gctccaacc cttttccaa attgagcaa 
      tgacgtgca ttacaaaga tccggccgg cacggttat 
      aacaataag gaacctcaa attaacgtt tttggacgt 
      gaattcccg acctcccca aaccacttt agctaggaa 
      atttttaaa ctcttcagt gagtgaatt gtcagactt 
      ccggactga gaatttcac caccagcga tcacacctt 
      gaacattac tttaacaac gccgtggtt tgaggcttg 
      gagagatac gtttttacg agttcagta tccctctga 
      tcatgaata tacaaagca ttttttatc gtgtgtttt 
      ggttgtttc ttcagctga aatcaatgt cattgaaag 
      tattattca gctgtctca gagtctgaa tcaaatgca 
      gaataacgc ccctggaaa gttacccaa gtggtttag 
      ctcaaatgt tttaaaata ctatgtgat catccatga 
      caatgttga aggaaatat tgatgtcgc aaatgaggc 
      gtacgagtc agttaaagg gtcatacgg ggaggcgaa 
      ttgttttct tttgaggat gaattcacg gcccccagg 
      tgccgcaaa caccgagca tttgccgga cccttggag 
      ccaattcat tagctgtaa tattgagtc cagagacta 
      caatccaac ccctgagag ctcacccta cacttcacg 
      ggcgatttt tgataccag tcaagactc tgatacaaa 
      aaccaactt aaattacca cccactgag ccctgacac 
      tgaggattt aataatgag cacttcggc aactgaaaa 
      agacaaaac cgtatcaaa caactccaa attaaaatc 
      gcacggtct ctgcctgtc gactgaaga cgtcgaaca 
      ggtgaaatt atttttaca cgagtttac caaaacctg 
      tctgctgcc ttacatcga gaaacaaat ccaccaact 
      aagcgacgt aatttcgaa caaaaaagg cgtcagcag 
      gtcattgtt cagtgcgac aaaacgctg gttcaaccc 
      caataaacc gggcgccag ttcggttgc agtgaataa 
      tcttatcta cgcgcctga ttcacccga attgcaagt 
      gcggcgact tggagatct ttatttcat gtttggaca 
      ttatctact agcgtttca agcatatca tttagctaa 
      aagggacta caacacgga tcaggcctg gctgtacta 
      cgctggggc gctcgaaat ggccgcctt ggcggcttt 
      tatggcgaa tgagtcgag cagaaagac ctacgatta 
      catggagga gagcataac cacttacat gaacacctg 
      caagatgcc tcagtttgc gacccgagc gacactttt 
      aagctccga gtgtctgaa aagtagggg tttgttcgg 
      cgaagcggc ccatcaatt gatacgaat gacgagtga 
      agagtcgga tttgagatc ggctctttt tctggaaca 
      ggccgctta ttgtttcct gcattcgaa aatggtcag 
      aaagtacgc ctttcatat ggttttggc cggccatag 
      attctcaaa agctgctca aaggaaaca ctcattgcg 
      gagctacaa acaagcaca ccaaattta cgaatacag 
      tggctggga tttagctga ggatcacat tcattatac 
      aatagggcg tcaagctca caatctcca ttcgtttga 
      tgaggcggt taagtcgtt tgcgcggct gcttaatgg 
      ggacagcaa gcagtcggg tcaacaaca gagcgtgtt 
      tttgaagga atatttaac aatattagg gaataaatt 
      actgaaaga ggatgacga cggtattcc gattttggc 
      tctaccgga gttagacag tgattcttt aaagttgtt 
      aatggagcc gacgcctcc cctaacaac acctgggaa 
      agttccggc aatgggtgt caattttct tgaaagaga 
      tggcgccga agttgcagc caggtggaa taaattgga 
      ggagatttt agtacaaga agccttcgg ttctcttcc 
      tctcacgtt caagccaat gatcacact ttacagcgt 
      ccataattt agcaaagaa cgtccccac agcgattgt 
      aaatgagcc gatccaagt ctgtgtgca actgattaa 
      ttcgttgca aatcgtcct acaattgaa agacattta 
      tttgatttg gtgcttcaa aaacgacga ccttgtggc 
      ttctaatga aaataacag caataacgt tgacaattt 
      cgtcaaaac gcacgtcac gaagtcgat tttgctaca 
      aagcaattg caatcaaaa tattatact tgcgttaac 
      gccgcttgt gacaggggt gatcacctt gaaagggat 
      ctgctacac gttaaccag ttccaacac gcctacaga 
      caatatatt tatcaaagg aaaacagtt gtttaattt 
      cgacaaaag cggcacgaa gaacgtaga aataaaagt 
      cgtgccgtt agcgccttg tttgcaggt gactttttc 
      cgagttcag tttggagtt tttatgcga cgaagtaca 
      aaaagttac aattgattt tcaaaatac gggtacgct 
      gcctttgca taaggtgta catggcgac gtcggcccc 
      cgagtactt atgtaactg tgagacaaa aaccaaaga 
      attgcccga attttcggc aaattgcac tccagcaat 
      gagggaaaa atttatcag ggataacca cattacttc 
      agtacctct tccaaacga ccgacttga gccggggca 
      aagcaccac ggtggccat atttatcaa agcgcctaa 
      tgttcccgg gccgtgtct tgtcgattt acttatcta 
      ttacgaaaa cgccaacgc gggagtcgt cccaaggta 
      tcggctcgt gcgccacaa gtggaactt gtcggtcca 
      ggagtccat caaagtgga cgtgacggt gcaagagag 
      tttcaatgc gaaacaagt ggaggagat cgcagtggt 
      gctgaaagc gtcaaactt gaacaaact ccacagctc 
      aacgctgac ggaaattag ccttttaaa cgtaggaat 
      gttgagtaa attctggag tttcatcga aaacgtggt 
      aacccccaa atacataaa cctacactc tcaggaatc 
      cgctcacat tttactaaa agcgcgccg acttaacga 
      aaaaaagag cgtctactc aaacatcta cttgaacca 
      tgagaggag catgcggca acacctcag ttccgcgta 
      catcgcctt cgccaaaaa gactgaaca ttgccaggt 
      gaatttgtt caacgagtt cgagtcttc tcctgaagg 
      caaggacgg gatcgtttt cgtgcaatg gcaagcgct 
      ggtgaacga cccgcagaa gcgcgtagt cgacggcca 
      aacgcagat ccggataaa agtccacaa gacgagcga 
      tgatcaaag cgacgtcga cgagtttgc ggctttgag 
      cgacccggc aactttgct cgaagtcag tttgaataa 
      agacaaaca gagcgagaa ccggacgca gcaaaccaa 
      agtcacatg cactctcat ctacccagc gatcgtcga 
      acacaattt ccagaagga gaaattctg tgtggttcc 
      ggtcactct cctcctcca cagcgttgt aaccgacgc 
      cgatttgag tgtgatagt caacacttt gggcgccac 
      gagtgggcc gccaccgcc gacacactc cgatctttt 
      ttatccgta tgcttccaa aaatttttc ctggcttgg 
      gacgggaag tgcagttag agaagtcaa gcctttggc 
      gacagaacc agtcaagct ctcggcagt tgtttcgag 
      gcccgggac ttttgattt aggggcgcg tattgagat 
      tttttgcag cagcaccga tcaagtcga cgctccgga 
      acttcaaac ctgccagat tcaatcagc attaattgt 
      tatagatgg ggatagtta acttcgata tttatttat 
      catgatttt ttgttaatg tatttgtat attttgaat 
      tttatcgtt tcagtctat tatgcttta taccatttt 
      tgtatatat ttacctgtt ttctttgta tctattaat 
      tccgatgta ttacaacaa aaattatta ataaaaaat 
      ttattacga c
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