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Gene information
General information
TC010851 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 5290065..5291249
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010851
    iBeetle screen iB_01747
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010851 RA
      MSDQNNCDL TTDAIPSPP RDEVKKEET 
      DETEPTNIP PKTKTKTRV FKKPKKSGP 
      SAFASVINI SNANGIHVG SNYNVYLSN 
      KSETQSQQQ HYIVTEAIR GLFHSDVPV 
      DRKDLIFLS PHINEHWRT MARALDFSD 
      GQVSQFYED YIRSGVKEV IYQLLLDWI 
      QNEADCATV GKLSKVLWD TNQQDAVQR 
      WSEYVIKNK S
    CDS sequence
    • >TC010851 RA
      atgtcagac cagaataac tgtgatctt acaactgat 
      gccataccc agccctcca agggatgaa gtcaaaaaa 
      gaagaaact gacgaaaca gaaccaacg aatatccct 
      ccaaaaaca aaaaccaaa actagagtt ttcaaaaaa 
      cccaaaaag agcggacca tctgctttt gccagtgtg 
      atcaatatt agtaacgca aatggaatt cacgtcggt 
      tcgaattat aacgtgtat ctcagtaat aaatcagag 
      actcaaagc caacaacaa cactacatt gtcacagag 
      gccatcaga ggcttgttc cacagtgac gtgcccgtt 
      gatcgaaaa gacttaatt tttctgtcc cctcatata 
      aacgaacac tggaggacc atggcaagg gcgctggat 
      ttctcagac ggacaagtc agccaattt tacgaagac 
      tacatcagg tctggagtt aaagaagtg atttaccag 
      ctcttactg gactggata caaaacgaa gccgattgt 
      gccactgtt ggaaaactc tcaaaagtg ttgtgggac 
      acaaaccag caagacgcc gttcaaagg tggtctgag 
      tatgttatc aaaaataaa tcgtag
    mRNA sequence
    • >TC010851 RA
      gtacttctc agtgatgta gcttgatca aatagctga 
      ctttcagac cctcaaatc attttgaac tagtttgta 
      caactttag tcatagaac gggttcaca cgaatgtaa 
      atatgtaaa taaaaattc cgcttcttt tttcaaatt 
      ctttgttta ttgcaagtg acatttgac gtaattccc 
      agacaaagg aaacaaatg tcaaaaatg atacatttg 
      ttggagagt taaaattta caacaaatc tgactgcaa 
      aaatgtcag accagaata actgtgatc ttacaactg 
      atgccatac ccagccctc caagggatg aagtcaaaa 
      aagaagaaa ctgacgaaa cagaaccaa cgaatatcc 
      ctccaaaaa caaaaacca aaactagag ttttcaaaa 
      aacccaaaa agagcggac catctgctt ttgccagtg 
      tgatcaata ttagtaacg caaatggaa ttcacgtcg 
      gttcgaatt ataacgtgt atctcagta ataaatcag 
      agactcaaa gccaacaac aacactaca ttgtcacag 
      aggccatca gaggcttgt tccacagtg acgtgcccg 
      ttgatcgaa aagacttaa tttttctgt cccctcata 
      taaacgaac actggagga ccatggcaa gggcgctgg 
      atttctcag acggacaag tcagccaat tttacgaag 
      actacatca ggtctggag ttaaagaag tgatttacc 
      agctcttac tggactgga tacaaaacg aagccgatt 
      gtgccactg ttggaaaac tctcaaaag tgttgtggg 
      acacaaacc agcaagacg ccgttcaaa ggtggtctg 
      agtatgtta tcaaaaata aatcgtagt ttttggtgc 
      tttttattg ttagttcag taataatgg ttctattct 
      tccaaccgc aatgtttct tatcattgt ttatttatt 
      gttatggaa agtttttcg tgacttttt atcattccg 
      cataaaaat tgtaatttg aatcgtgtg atatcttca 
      ggccagtgg cactggttt aaatggcgt aatcatgtg 
      gctaatttt tcaagtatt aagccatat atttgtact 
      ttgttagaa ataa