Status
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Gene information
General information
TC010839 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5979454..5985183
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010839
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01744

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010839 RA
      MWSKIVLCF FVGLACASH NPGWQHNHE YVYQIRGRS 
      LTGLDQISS NHAGMLFKA KLWVRPRSD GHLGARIIE 
      PQYAEIHSK LYRSWDQEI PDSELSYSP ISLSSKPFL 
      IKMDNNAVS DLVVDRSIS NWEANMIKA IVSQLQLDT 
      NAENLIPSS INILTQEGS STAVFKTME PTVTGESET 
      LYEIHPLPD YILQTTPWL APLKHLKGR GQVIEVVKN 
      KNFTHSKET PSYHFGFHG VDRFDIDNQ IGEFFSRTS 
      VSRAVITGN LDSFTLQSS VTVDEVTVN PSMQDKQRG 
      STRSIVNIT LVSMDEQNQ EFEQVSNPV NVGLVYHYD 
      NPFDQTNEP HLRSQIRSG RKNSDESSS SHSQESSNQ 
      IRRHKRSLS HASSESDES RSEELYSSV QEKPHINEA 
      PASPLLPFT VGYDGQAIS KKQNIKESA RNLAKKIGQ 
      EFQQSQNIP ERNTVGKFL ILSSLVKVM DKDEMKQVA 
      DQLYTQEQH GAAAAAWVA YRDAVAEAG TGPALLNIQ 
      EWIREGKIS REEAAEVVS TAAVSARQP TEKYMKVYF 
      EMIQNDQIM EQPYLNESL LLAYTTLIH QVYVNEEYS 
      HSKYPVHSF GSFNTAQGW FYVKKEVIP YLSQKLDEA 
      ISNADTKQI HLYIRALGN VGHQQILQS FKPYLEGTK 
      KASHFQRTL MVVALDKLA KSNPQVARS VLYKIYQNA 
      HDFDQVRVA AVYQLMKSQ PSAAMLQRM AYYTNVDTS 
      NQVNAAVKS IIESACELQ GPRYEKIRS AAYSAKPLL 
      TSQDFGVQH SHGNLRSFV EQEMRSEYL QKFNYLVGE 
      DSVLPKGIN YYLQGRFGG VRRFVVDMQ SMVSSIEDL 
      INVFEQQTK EYRRLLKEK QAAQSANTK WSSEHTANL 
      FNMQNEQRE QLEGNLYFH VDALQKMWS FDNQTIENL 
      LQIVRKCEQ DLKNGKHID YNKMSVNYE TALSFPSAW 
      GLPLIYTFD VPTLVHIES EMQASANPS VYSEQGLQQ 
      PETVDLEYK INAFVSGRV QSQLGFFAP FNHKRYSSG 
      YDKNFQVVV PQISGKVHL NVENKQATL EAENTDSQK 
      NTNLVHYSS WPYTSVYEI FDVEPKNQI IEKENPHRV 
      NTIIGDKST GIAFYFKMI SSRPIDCGF MYDRLRKQD 
      LVSALLGSW VDDNMQHIQ IDLGIDSDK STTNKVVVH 
      LGHQHTYNS NLNRQPAPE MSSRYQAAQ ESWERQKEF 
      LKNCSKDIR AVRAHVVDA IVLFEGQRI VKYTSTLAG 
      ARSDIDPRG RVLMQMEKD NQHDKPMRV FFAANTKIP 
      NTNSLNLKY ALEFDPTST IQIQGVIEK KSGNDAQIN 
      AKLTLAKSE ERKQYLKEL PQYEKCQKE MQEGNYQLP 
      ACAMLTAQA NLLDQFSFE MNYHNIDKV VVNSTYKLY 
      TVLRHYMYP RVSEDILME NEQVQKDRL QVKGQFSPC 
      LSRVNASVV TEYGKVDFN NVKVQDWAK ALLVSHPVH 
      HVKSRLGGY ALNYETYKA NCMVDSCAV NTLDNRTFP 
      IDLSKDWVV LLHYVPRRP SPIKNQPYL TVPEQLNQQ 
      VEGYIVYGR SYGDAKKEI KMVIQSPDT LSKVIDISL 
      KPSESSSYP RLFVQGQEI KYDDQGSHM YDGYMQAYR 
      LPNQETKVQ VYNAFSVIY DGVRAKIEI TNDKFRDAA 
      RGLCGTFTN EQETDFTHP GNCIIHNPE VFVATNTVP 
      EKQRTKRMN EMMMIQAEE KKCYRKSVQ FVKIVSAQD 
      SGIMGGEMY AREGIMYRT QYALAAGQT CFSISPVPV 
      CKDGYKQVE SLNRPVPAH CVESSQLAR SLRRQIDQG 
      ANPSFKSKP QHREVMMQM AKRCEA
    CDS sequence
    • >TC010839 RA
      atgtggtcc aaaatagtg ctttgtttt tttgtgggc 
      ctcgcctgc gctagccac aacccaggc tggcaacac 
      aaccacgaa tacgtctac caaatccgc ggccgctct 
      ctcaccggt ttggaccaa atcagcagc aatcatgcc 
      ggtatgctc tttaaagcc aaactttgg gtaagaccc 
      cgatctgac ggacacctc ggagcccga atcatcgaa 
      ccacaatat gccgaaatc cactcgaag ctttaccga 
      agctgggat caggaaatc ccggattcg gaactcagc 
      tacagtccg atttccctc tcatcgaag ccgtttttg 
      atcaaaatg gacaacaac gccgtcagt gaccttgtt 
      gttgaccgg tcgatctca aactgggaa gctaacatg 
      atcaaagcc attgtgagt caactccag ttggacaca 
      aacgccgaa aatcttatt cccagctcg atcaacatt 
      ttgactcaa gaggggtcg agcacggct gttttcaaa 
      acaatggaa cccactgtg accggagag tccgaaact 
      ttgtacgaa atccacccc ttgcccgat tatatcctc 
      cagacaact ccatggttg gctcctttg aaacatttg 
      aaaggcaga ggacaagtt atcgaagtt gtgaaaaac 
      aaaaatttc acacacagc aaggaaact ccctcgtat 
      cactttggg ttccatgga gtcgaccgt tttgacatt 
      gataaccaa atcggggaa tttttctcc cgtacttcg 
      gtctcccgt gctgtgatt accggaaat ttggatagt 
      ttcaccttg caaagctca gtgaccgtc gatgaagtc 
      acagttaac ccctccatg caagacaaa caaagaggc 
      tcaaccagg agcattgtc aacatcact ttggtctca 
      atggatgag caaaaccaa gaatttgaa caagtgtca 
      aaccccgtc aatgttggt cttgtctat cactacgac 
      aaccctttc gaccaaacc aacgaaccc catctcaga 
      tctcaaatt cgcagtgga aggaaaaac agcgatgaa 
      tcttcctcc tctcacagc caggaatct tccaaccaa 
      atccgaaga cacaaacgt agcttgtct cacgcttcg 
      tccgaatcc gacgaatca aggtccgag gaactttac 
      tcctcagtt caggaaaaa ccccacatt aatgaagca 
      cctgcctca cctttgctc cccttcact gtcggttac 
      gacgggcaa gccatcagc aaaaagcaa aacatcaaa 
      gaaagtgcc cgcaatcta gccaaaaag atcggccag 
      gagttccaa cagtcgcaa aacatcccc gaacgaaac 
      accgtcggc aagttcctg attttgagc agccttgtg 
      aaagttatg gataaggat gagatgaaa caagtcgct 
      gaccagttg tacacacaa gagcaacat ggagctgcc 
      gccgccgcc tgggtcgca taccgcgac gctgtcgca 
      gaggctgga acaggacct gccctcctc aacatccag 
      gagtggatc cgcgaagga aaaatctcc cgggaagaa 
      gccgcggaa gtcgtgtca acggctgcc gtttccgcc 
      agacaacca accgagaaa tacatgaag gtctatttc 
      gagatgatt caaaacgac caaatcatg gaacaaccc 
      tacttgaac gagtccttg ctcttggct tacaccact 
      ttgatccac caagtttac gtcaacgag gaatattcg 
      cacagtaaa taccccgtc catagtttc ggctcgttt 
      aacacagct caagggtgg ttctacgtc aagaaggaa 
      gtcatccct tacttgtcc caaaaactg gacgaagct 
      atttctaac gctgacacc aaacaaatc catttgtac 
      atccgtgcc cttggaaac gtcggacac cagcaaatc 
      ttgcaatcg ttcaaaccc tatttggaa ggaaccaag 
      aaagcctcc cacttccag aggactttg atggttgtg 
      gctttggat aaactggcg aagagtaac cctcaagtc 
      gcccgctcc gttttgtac aaaatctac caaaacgca 
      cacgatttc gaccaagtg cgtgttgct gccgtctat 
      caattgatg aaatcgcaa ccctcagct gcaatgctc 
      caacgcatg gcctattac accaatgtg gacacgagc 
      aaccaagtt aatgctgcc gtaaaatca atcatcgaa 
      tcagcctgc gagcttcaa ggaccacgt tacgaaaaa 
      atacgttcg gctgcctat tctgctaaa cccctcctc 
      acttcgcaa gactttggc gtccaacac tcccacggc 
      aatttgcgt agctttgtt gagcaggaa atgcgatca 
      gaatatttg caaaaattc aactacctt gtcggcgaa 
      gacagcgtt ttgcccaaa ggaattaac tactacctc 
      caaggacgc ttcggtgga gtcagacgt tttgtcgtc 
      gacatgcaa tcaatggtg tcaagtatt gaagacttg 
      atcaacgtt ttcgaacaa caaaccaaa gaataccgc 
      cgcttgttg aaggaaaaa caagccgcc caaagtgcc 
      aacaccaaa tggtcaagc gaacatacc gccaacctt 
      ttcaacatg caaaacgaa caacgggaa caactcgaa 
      ggaaatctc tacttccac gttgatgct ttgcaaaaa 
      atgtggtcg tttgataac caaaccatc gaaaatttg 
      ttgcaaatt gtcagaaag tgtgagcaa gacttgaaa 
      aacggcaaa cacatcgat tacaacaaa atgagcgtc 
      aattacgaa accgccttg tcgttcccc agcgcttgg 
      ggcttgccc ttgatctac accttcgac gtgcccacc 
      cttgtccac atcgaatca gagatgcaa gcttcagct 
      aacccttca gtctactcg gagcaaggt ctccaacag 
      cccgaaacc gtcgatctc gaatacaaa atcaacgcg 
      ttcgtttcg ggacgtgtc caaagtcag cttggtttc 
      ttcgctcct ttcaaccac aaacgctac agttctggc 
      tacgacaag aacttccaa gtcgttgtg ccccaaatt 
      tccggcaag gtgcacctc aacgtcgaa aacaaacaa 
      gccaccttg gaagctgaa aacaccgac agccagaaa 
      aacacaaac ttggtccat tacagctcc tggccgtac 
      acctccgtc tacgaaatt ttcgacgtc gagcccaaa 
      aaccaaatt attgagaag gaaaacccc catcgagtt 
      aacaccatt attggggac aaatccaca ggaattgcc 
      ttctatttc aagatgatc agcagccgc cctattgat 
      tgtgggttt atgtacgat aggctccgc aaacaggac 
      ctggtttcg gcccttttg gggtcatgg gttgatgat 
      aacatgcaa cacatccaa attgattta ggaattgac 
      tctgacaag tcgaccact aataaggtt gttgtccat 
      ttgggccac caacacacc tacaattcg aatttgaac 
      cgccaacca gcccccgaa atgtcatcc cggtaccaa 
      gccgctcaa gagtcgtgg gaaaggcag aaggaattt 
      ttgaaaaac tgcagcaag gacattcgt gccgtccgt 
      gcccacgtt gttgacgca attgtgttg ttcgaaggg 
      caaagaata gttaaatac acctcaacc cttgccggc 
      gctcggagc gacattgat ccccgaggt cgcgttttg 
      atgcaaatg gaaaaagac aaccagcac gacaaaccc 
      atgagggtc ttcttcgcc gctaacacc aaaatacca 
      aacaccaac agtctcaac ttgaaatat gccttggaa 
      tttgaccca actagcacc atccagatc caaggcgtc 
      atcgaaaag aagagtggc aacgacgcc caaattaat 
      gccaagttg actttggcc aagagcgag gagcgcaaa 
      caatatttg aaggaattg ccacagtat gaaaaatgc 
      cagaaggag atgcaggaa ggtaactac caactgccc 
      gcatgtgcc atgctgaca gcccaagcc aatcttttg 
      gatcaattc tcattcgaa atgaattat cacaacatt 
      gacaaggtt gtggttaac agcacatac aaattgtac 
      accgttttg cgccactac atgtaccca agagtttcg 
      gaagacatc ctgatggaa aatgagcaa gtgcagaag 
      gatcgtctt caggtcaag ggtcaattt agcccgtgt 
      ttgtctcgt gtcaatgca tctgttgtc accgagtac 
      ggaaaagtt gacttcaac aacgtcaaa gtccaggat 
      tgggccaaa gcccttttg gtgtcgcat ccagtacac 
      catgtcaaa tctcgtcta ggcggatac gcccttaac 
      tatgagact tacaaggca aactgtatg gttgacagt 
      tgcgctgtt aacactttg gacaacagg acattcccc 
      attgactta tccaaggac tgggtcgtc ctcctccac 
      tatgtcccg cgcagacct tccccaatc aaaaaccag 
      ccctacttg accgtcccc gaacaactg aaccaacaa 
      gtcgaaggt tacatcgtc tatggtcgt tcatacggt 
      gacgccaaa aaggaaatc aaaatggtc atccaatca 
      ccggatacc ctatccaag gtgatcgac atttcgctg 
      aaacctagc gaatcctca agctacccg cgcctcttc 
      gtccagggc caggaaatc aagtacgac gaccaaggt 
      tcccacatg tacgacggt tacatgcaa gcgtacaga 
      cttccaaac caggaaacc aaagtccaa gtctacaac 
      gccttcagc gtgatctac gacggcgtc cgcgccaaa 
      atcgaaatc accaacgac aaattccgc gatgccgcc 
      cgcggcctc tgcggcacg ttcaccaac gagcaagaa 
      accgacttc acccacccc gggaactgc atcatccac 
      aaccccgag gtcttcgtc gccactaac accgtgcct 
      gagaagcag cgcacgaag aggatgaac gaaatgatg 
      atgatccaa gccgaggag aagaagtgc taccgcaaa 
      tcggtgcaa ttcgtcaag atcgtctca gcgcaggat 
      tccgggatc atgggaggt gagatgtac gcccgggaa 
      gggatcatg tacaggacg cagtatgcg ctagcagcg 
      ggccaaacc tgcttcagc atttcgccg gtcccggtg 
      tgcaaagac gggtacaag caagtcgag agtcttaac 
      aggcctgtg cccgcacac tgcgtggaa tcgtcgcag 
      cttgcgagg tcgctgagg aggcagatc gatcagggg 
      gctaatccc agcttcaaa tcgaagccg cagcacagg 
      gaagttatg atgcagatg gcgaaaaga tgcgaagcc 
      taa
    mRNA sequence
    • >TC010839 RA
      tgcgactga ttattgcgt cgcgatgtg gtccaaaat 
      agtgctttg tttttttgt gggcctcgc ctgcgctag 
      ccacaaccc aggctggca acacaacca cgaatacgt 
      ctaccaaat ccgcggccg ctctctcac cggtttgga 
      ccaaatcag cagcaatca tgccggtat gctctttaa 
      agccaaact ttgggtaag accccgatc tgacggaca 
      cctcggagc ccgaatcat cgaaccaca atatgccga 
      aatccactc gaagcttta ccgaagctg ggatcagga 
      aatcccgga ttcggaact cagctacag tccgatttc 
      cctctcatc gaagccgtt tttgatcaa aatggacaa 
      caacgccgt cagtgacct tgttgttga ccggtcgat 
      ctcaaactg ggaagctaa catgatcaa agccattgt 
      gagtcaact ccagttgga cacaaacgc cgaaaatct 
      tattcccag ctcgatcaa cattttgac tcaagaggg 
      gtcgagcac ggctgtttt caaaacaat ggaacccac 
      tgtgaccgg agagtccga aactttgta cgaaatcca 
      ccccttgcc cgattatat cctccagac aactccatg 
      gttggctcc tttgaaaca tttgaaagg cagaggaca 
      agttatcga agttgtgaa aaacaaaaa tttcacaca 
      cagcaagga aactccctc gtatcactt tgggttcca 
      tggagtcga ccgttttga cattgataa ccaaatcgg 
      ggaattttt ctcccgtac ttcggtctc ccgtgctgt 
      gattaccgg aaatttgga tagtttcac cttgcaaag 
      ctcagtgac cgtcgatga agtcacagt taacccctc 
      catgcaaga caaacaaag aggctcaac caggagcat 
      tgtcaacat cactttggt ctcaatgga tgagcaaaa 
      ccaagaatt tgaacaagt gtcaaaccc cgtcaatgt 
      tggtcttgt ctatcacta cgacaaccc tttcgacca 
      aaccaacga accccatct cagatctca aattcgcag 
      tggaaggaa aaacagcga tgaatcttc ctcctctca 
      cagccagga atcttccaa ccaaatccg aagacacaa 
      acgtagctt gtctcacgc ttcgtccga atccgacga 
      atcaaggtc cgaggaact ttactcctc agttcagga 
      aaaacccca cattaatga agcacctgc ctcaccttt 
      gctcccctt cactgtcgg ttacgacgg gcaagccat 
      cagcaaaaa gcaaaacat caaagaaag tgcccgcaa 
      tctagccaa aaagatcgg ccaggagtt ccaacagtc 
      gcaaaacat ccccgaacg aaacaccgt cggcaagtt 
      cctgatttt gagcagcct tgtgaaagt tatggataa 
      ggatgagat gaaacaagt cgctgacca gttgtacac 
      acaagagca acatggagc tgccgccgc cgcctgggt 
      cgcataccg cgacgctgt cgcagaggc tggaacagg 
      acctgccct cctcaacat ccaggagtg gatccgcga 
      aggaaaaat ctcccggga agaagccgc ggaagtcgt 
      gtcaacggc tgccgtttc cgccagaca accaaccga 
      gaaatacat gaaggtcta tttcgagat gattcaaaa 
      cgaccaaat catggaaca accctactt gaacgagtc 
      cttgctctt ggcttacac cactttgat ccaccaagt 
      ttacgtcaa cgaggaata ttcgcacag taaataccc 
      cgtccatag tttcggctc gtttaacac agctcaagg 
      gtggttcta cgtcaagaa ggaagtcat cccttactt 
      gtcccaaaa actggacga agctatttc taacgctga 
      caccaaaca aatccattt gtacatccg tgcccttgg 
      aaacgtcgg acaccagca aatcttgca atcgttcaa 
      accctattt ggaaggaac caagaaagc ctcccactt 
      ccagaggac tttgatggt tgtggcttt ggataaact 
      ggcgaagag taaccctca agtcgcccg ctccgtttt 
      gtacaaaat ctaccaaaa cgcacacga tttcgacca 
      agtgcgtgt tgctgccgt ctatcaatt gatgaaatc 
      gcaaccctc agctgcaat gctccaacg catggccta 
      ttacaccaa tgtggacac gagcaacca agttaatgc 
      tgccgtaaa atcaatcat cgaatcagc ctgcgagct 
      tcaaggacc acgttacga aaaaatacg ttcggctgc 
      ctattctgc taaacccct cctcacttc gcaagactt 
      tggcgtcca acactccca cggcaattt gcgtagctt 
      tgttgagca ggaaatgcg atcagaata tttgcaaaa 
      attcaacta ccttgtcgg cgaagacag cgttttgcc 
      caaaggaat taactacta cctccaagg acgcttcgg 
      tggagtcag acgttttgt cgtcgacat gcaatcaat 
      ggtgtcaag tattgaaga cttgatcaa cgttttcga 
      acaacaaac caaagaata ccgccgctt gttgaagga 
      aaaacaagc cgcccaaag tgccaacac caaatggtc 
      aagcgaaca taccgccaa ccttttcaa catgcaaaa 
      cgaacaacg ggaacaact cgaaggaaa tctctactt 
      ccacgttga tgctttgca aaaaatgtg gtcgtttga 
      taaccaaac catcgaaaa tttgttgca aattgtcag 
      aaagtgtga gcaagactt gaaaaacgg caaacacat 
      cgattacaa caaaatgag cgtcaatta cgaaaccgc 
      cttgtcgtt ccccagcgc ttggggctt gcccttgat 
      ctacacctt cgacgtgcc cacccttgt ccacatcga 
      atcagagat gcaagcttc agctaaccc ttcagtcta 
      ctcggagca aggtctcca acagcccga aaccgtcga 
      tctcgaata caaaatcaa cgcgttcgt ttcgggacg 
      tgtccaaag tcagcttgg tttcttcgc tcctttcaa 
      ccacaaacg ctacagttc tggctacga caagaactt 
      ccaagtcgt tgtgcccca aatttccgg caaggtgca 
      cctcaacgt cgaaaacaa acaagccac cttggaagc 
      tgaaaacac cgacagcca gaaaaacac aaacttggt 
      ccattacag ctcctggcc gtacacctc cgtctacga 
      aattttcga cgtcgagcc caaaaacca aattattga 
      gaaggaaaa cccccatcg agttaacac cattattgg 
      ggacaaatc cacaggaat tgccttcta tttcaagat 
      gatcagcag ccgccctat tgattgtgg gtttatgta 
      cgataggct ccgcaaaca ggacctggt ttcggccct 
      tttggggtc atgggttga tgataacat gcaacacat 
      ccaaattga tttaggaat tgactctga caagtcgac 
      cactaataa ggttgttgt ccatttggg ccaccaaca 
      cacctacaa ttcgaattt gaaccgcca accagcccc 
      cgaaatgtc atcccggta ccaagccgc tcaagagtc 
      gtgggaaag gcagaagga atttttgaa aaactgcag 
      caaggacat tcgtgccgt ccgtgccca cgttgttga 
      cgcaattgt gttgttcga agggcaaag aatagttaa 
      atacacctc aacccttgc cggcgctcg gagcgacat 
      tgatccccg aggtcgcgt tttgatgca aatggaaaa 
      agacaacca gcacgacaa acccatgag ggtcttctt 
      cgccgctaa caccaaaat accaaacac caacagtct 
      caacttgaa atatgcctt ggaatttga cccaactag 
      caccatcca gatccaagg cgtcatcga aaagaagag 
      tggcaacga cgcccaaat taatgccaa gttgacttt 
      ggccaagag cgaggagcg caaacaata tttgaagga 
      attgccaca gtatgaaaa atgccagaa ggagatgca 
      ggaaggtaa ctaccaact gcccgcatg tgccatgct 
      gacagccca agccaatct tttggatca attctcatt 
      cgaaatgaa ttatcacaa cattgacaa ggttgtggt 
      taacagcac atacaaatt gtacaccgt tttgcgcca 
      ctacatgta cccaagagt ttcggaaga catcctgat 
      ggaaaatga gcaagtgca gaaggatcg tcttcaggt 
      caagggtca atttagccc gtgtttgtc tcgtgtcaa 
      tgcatctgt tgtcaccga gtacggaaa agttgactt 
      caacaacgt caaagtcca ggattgggc caaagccct 
      tttggtgtc gcatccagt acaccatgt caaatctcg 
      tctaggcgg atacgccct taactatga gacttacaa 
      ggcaaactg tatggttga cagttgcgc tgttaacac 
      tttggacaa caggacatt ccccattga cttatccaa 
      ggactgggt cgtcctcct ccactatgt cccgcgcag 
      accttcccc aatcaaaaa ccagcccta cttgaccgt 
      ccccgaaca actgaacca acaagtcga aggttacat 
      cgtctatgg tcgttcata cggtgacgc caaaaagga 
      aatcaaaat ggtcatcca atcaccgga taccctatc 
      caaggtgat cgacatttc gctgaaacc tagcgaatc 
      ctcaagcta cccgcgcct cttcgtcca gggccagga 
      aatcaagta cgacgacca aggttccca catgtacga 
      cggttacat gcaagcgta cagacttcc aaaccagga 
      aaccaaagt ccaagtcta caacgcctt cagcgtgat 
      ctacgacgg cgtccgcgc caaaatcga aatcaccaa 
      cgacaaatt ccgcgatgc cgcccgcgg cctctgcgg 
      cacgttcac caacgagca agaaaccga cttcaccca 
      ccccgggaa ctgcatcat ccacaaccc cgaggtctt 
      cgtcgccac taacaccgt gcctgagaa gcagcgcac 
      gaagaggat gaacgaaat gatgatgat ccaagccga 
      ggagaagaa gtgctaccg caaatcggt gcaattcgt 
      caagatcgt ctcagcgca ggattccgg gatcatggg 
      aggtgagat gtacgcccg ggaagggat catgtacag 
      gacgcagta tgcgctagc agcgggcca aacctgctt 
      cagcatttc gccggtccc ggtgtgcaa agacgggta 
      caagcaagt cgagagtct taacaggcc tgtgcccgc 
      acactgcgt ggaatcgtc gcagcttgc gaggtcgct 
      gaggaggca gatcgatca gggggctaa tcccagctt 
      caaatcgaa gccgcagca cagggaagt tatgatgca 
      gatggcgaa aagatgcga agcctaagc ggcgcgaaa 
      attgtagat agatattta tatgagcat tcctaaaat 
      gtaaaaaaa agtaataaa aatttggac cattt
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