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Gene information
General information
TC010837 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6016722..6017762
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010837
    iBeetle screen iB_01743

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Fertility
    egg chamber complete not present - Number of animals showing the phenotype: 5

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    vitellogenic egg chamber not present - Number of animals showing the phenotype: 6

    Comment: Probably compound egg chambers!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010837 RA
      MGNKLLTFS EQQLENYQD CTFFTRKEI 
      LRVHKKFRN IRPDLIPKR MRQNEQVTI 
      KVPLEDIVE KLPELRENP FKKRICEVF 
      SRDGKGNLS FEDILDLLS VFSEQAPRD 
      IKVFYAFSI YDFDNDQYI GSDDLDNTL 
      LLLMHQELT PEDRQQIIE KVIEEADVD 
      GDGKLSFME FEHVITRAP DFLSTFHIR 
      I
    CDS sequence
    • >TC010837 RA
      atgggaaat aaattattg acattttcg gaacaacaa 
      ttagagaat tatcaggac tgtactttc tttacgaga 
      aaggagatt ttaagagtc cacaaaaaa ttcagaaat 
      attcgcccc gatttaatc ccgaaacgt atgcggcaa 
      aacgagcaa gtcacgatt aaagtacca ctggaggat 
      attgttgaa aaattaccc gaactgcgg gaaaatcct 
      ttcaaaaaa cgcatttgc gaagtgttc tcccgcgac 
      gggaaagga aatttatct ttcgaagac attctggac 
      ttgctttca gtgttcagc gagcaagct ccaagggac 
      ataaaggtt ttttatgcg ttcagtata tatgatttc 
      gataatgac cagtatatc gggtccgac gatttagac 
      aacacgctg cttttgctc atgcatcag gaactaaca 
      cctgaggat cggcagcaa atcatcgaa aaggttata 
      gaggaggca gacgttgac ggggacggg aagttatct 
      ttcatggag ttcgagcat gtgattacg cgcgcaccc 
      gattttctc tccactttt cacattcgg atatag
    mRNA sequence
    • >TC010837 RA
      ttagtttgt agcacagct cttatcggt gatgtttcg 
      tgtttccta aaccaccat gggaaataa attattgac 
      attttcgga acaacaatt agagaatta tcaggactg 
      tactttctt tacgagaaa ggagatttt aagagtcca 
      caaaaaatt cagaaatat tcgccccga tttaatccc 
      gaaacgtat gcggcaaaa cgagcaagt cacgattaa 
      agtaccact ggaggatat tgttgaaaa attacccga 
      actgcggga aaatccttt caaaaaacg catttgcga 
      agtgttctc ccgcgacgg gaaaggaaa tttatcttt 
      cgaagacat tctggactt gctttcagt gttcagcga 
      gcaagctcc aagggacat aaaggtttt ttatgcgtt 
      cagtatata tgatttcga taatgacca gtatatcgg 
      gtccgacga tttagacaa cacgctgct tttgctcat 
      gcatcagga actaacacc tgaggatcg gcagcaaat 
      catcgaaaa ggttataga ggaggcaga cgttgacgg 
      ggacgggaa gttatcttt catggagtt cgagcatgt 
      gattacgcg cgcacccga ttttctctc cacttttca 
      cattcggat atagccaaa tcaatgatc agaaacaat 
      aaaatttat ttatgcttc aaattacat ctcatatgt 
      acatccagg aggagaaat ataagaata taacagtaa 
      tatgaagaa caaaaggca cgtataaca cacacgaag 
      tgattgtcg ccaagtcac aatcatgaa gtaacgaaa 
      taacgaata gaaaagtag ttttattag tctcacagc 
      tacatcgta ttaagattt ctttgttga ttattgtat 
      tgaacattt atgaataaa ccacagttg gccgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY