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Gene information
General information
TC010831 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6113389..6116884
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010831
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01742

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    pupal molt delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    antenna orientation bent - Number of animals showing the phenotype: 9

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010831 RA
      MTSQNSPFP QRYQHIFDY SQQTDGQWD PMTTSHPPT 
      VPARGIKRS TPSEAFRPH DDPSWMASQ EVASQMDNS 
      IKHECLRSP NNMNISSDC NNFKNVRHD VNVNHVPSL 
      MRPNSKFST TPSWFHGNI SRENAENLL SARTDGLFL 
      VRESTNFPG DYTLCVCYD NKVEHYRVK SNGGKLTID 
      DEEFFDNLE DMIEHYKND ADGLCTKLV KALCNEKSK 
      ENDDLCVIS EQELEICEP IGKGEFCEV MLGKWKKNK 
      VAVKVLKDS SEAEAILMK SLHHENLVN LLGIVRKKD 
      QIYLVTEYM SKGSLVDYL RSRGRLHVT KKDQINFAF 
      DTSSGMEYL EKMHVVHRD LAARNVLIA ENGVAKVSD 
      FGLARNEMN PMSESAKLP IKWTAPEAL KRNIFSNKS 
      DMWSFGILL WEIYSFGRV PYPRIPLAD VVKHVEKGY 
      KMEAPEGCP SEVYEIMRQ AWDLNPDKR PTFQDVKAK 
      LAQLKQQTS
    • >TC010831 RB
      MTSQNSPFP QVRHLNPSR IEYVNQVGS LQRYQHIFD 
      YSQQTDGQW DPMTTSHPP TVPARGIKR STPSEAFRP 
      HDDPSWMAS QEVASQMDN SIKHECLRS PNNMNISSD 
      CNNFKNVRH DVNVNHVPS LMRPNSKFS TTPSWFHGN 
      ISRENAENL LSARTDGLF LVRESTNFP GDYTLCVCY 
      DNKVEHYRV KSNGGKLTI DDEEFFDNL EDMIEHYKN 
      DADGLCTKL VKALCNEKS KENDDLCVI SEQELEICE 
      PIGKGEFCE VMLGKWKKN KVAVKVLKD SSEAEAILM 
      KSLHHENLV NLLGIVRKK DQIYLVTEY MSKGSLVDY 
      LRSRGRLHV TKKDQINFA FDTSSGMEY LEKMHVVHR 
      DLAARNVLI AENGVAKVS DFGLARNEM NPMSESAKL 
      PIKWTAPEA LKRNIFSNK SDMWSFGIL LWEIYSFGR 
      VPYPRIPLA DVVKHVEKG YKMEAPEGC PSEVYEIMR 
      QAWDLNPDK RPTFQDVKA KLAQLKQQT S
    CDS sequence
    • >TC010831 RA
      atgaccagc caaaactcg ccatttcct cagaggtat 
      cagcacatt ttcgactac agtcaacaa acggacggt 
      caatgggac cccatgacc acctcacac ccacccaca 
      gttcctgcc aggggcatc aagaggagc acccctagc 
      gaagcgttc cgtccacat gatgaccct tcctggatg 
      gcgtcccaa gaagtggcg tcacaaatg gacaacagt 
      atcaagcac gaatgtctg agatccccg aataacatg 
      aacattagt tcagattgc aacaatttc aaaaatgtg 
      cgccatgac gtcaacgtt aaccatgtc cccagttta 
      atgcgaccg aattcgaaa ttcagtaca acaccgagt 
      tggtttcac ggcaacatt tcgcgcgag aacgccgaa 
      aatttgctg tcagcgcgc accgatggg ctgtttctc 
      gtgcgggaa tcaacgaat ttcccgggc gattacact 
      ctgtgtgtc tgctacgac aacaaagtc gagcattac 
      agagtgaag tcgaacgga ggtaaatta acaatcgac 
      gacgaggag ttttttgat aatctcgaa gacatgatc 
      gaacattac aaaaacgac gccgacggt ctctgtacg 
      aagttagtg aaagcgttg tgcaacgaa aagtcgaag 
      gaaaacgac gacttgtgc gtcatctct gaacaggag 
      ctcgagatc tgcgagcca atcggcaag ggcgagttt 
      tgcgaggtg atgctgggt aagtggaag aagaacaag 
      gtggcggtg aaggtgctg aaggactcg agcgaggca 
      gaggcaatt ttaatgaaa tcgctccac cacgagaat 
      ttggttaat ttgttaggc attgtgcgg aagaaagat 
      cagatttat ttggtgacg gagtatatg agtaaaggc 
      agtctagtt gattatttg cggtcgagg gggcgtttg 
      cacgtcacg aagaaggac cagatcaac tttgcgttt 
      gacacaagc tcgggtatg gagtatttg gagaaaatg 
      catgtggtg cacagggat ttagctgcg aggaatgtt 
      ctcatcgcc gaaaacggc gtagcgaaa gtgtccgat 
      tttgggttg gcgcggaat gagatgaat cccatgtcg 
      gagtcggcg aagttgccg atcaaatgg acggctccc 
      gaggctttg aagcgaaat atattttcg aacaagtcg 
      gacatgtgg agcttcggg attctctta tgggagatt 
      tactccttc ggtagagtt ccgtacccg agaatacct 
      cttgcggac gtggtgaag cacgtggag aagggatac 
      aagatggag gctccggaa ggctgtccg tcggaagtt 
      tacgaaatt atgagacag gcgtgggat ctgaatccg 
      gacaaaaga ccgacgttt caagacgtg aaggcgaaa 
      cttgcacaa ttgaagcaa cagacttcc tga
    • >TC010831 RB
      atgaccagc caaaactcg ccatttcct caggtgcgt 
      catttaaac ccatcgcgt attgaatac gtaaatcag 
      gttgggtcg ttgcagagg tatcagcac attttcgac 
      tacagtcaa caaacggac ggtcaatgg gaccccatg 
      accacctca cacccaccc acagttcct gccaggggc 
      atcaagagg agcacccct agcgaagcg ttccgtcca 
      catgatgac ccttcctgg atggcgtcc caagaagtg 
      gcgtcacaa atggacaac agtatcaag cacgaatgt 
      ctgagatcc ccgaataac atgaacatt agttcagat 
      tgcaacaat ttcaaaaat gtgcgccat gacgtcaac 
      gttaaccat gtccccagt ttaatgcga ccgaattcg 
      aaattcagt acaacaccg agttggttt cacggcaac 
      atttcgcgc gagaacgcc gaaaatttg ctgtcagcg 
      cgcaccgat gggctgttt ctcgtgcgg gaatcaacg 
      aatttcccg ggcgattac actctgtgt gtctgctac 
      gacaacaaa gtcgagcat tacagagtg aagtcgaac 
      ggaggtaaa ttaacaatc gacgacgag gagtttttt 
      gataatctc gaagacatg atcgaacat tacaaaaac 
      gacgccgac ggtctctgt acgaagtta gtgaaagcg 
      ttgtgcaac gaaaagtcg aaggaaaac gacgacttg 
      tgcgtcatc tctgaacag gagctcgag atctgcgag 
      ccaatcggc aagggcgag ttttgcgag gtgatgctg 
      ggtaagtgg aagaagaac aaggtggcg gtgaaggtg 
      ctgaaggac tcgagcgag gcagaggca attttaatg 
      aaatcgctc caccacgag aatttggtt aatttgtta 
      ggcattgtg cggaagaaa gatcagatt tatttggtg 
      acggagtat atgagtaaa ggcagtcta gttgattat 
      ttgcggtcg agggggcgt ttgcacgtc acgaagaag 
      gaccagatc aactttgcg tttgacaca agctcgggt 
      atggagtat ttggagaaa atgcatgtg gtgcacagg 
      gatttagct gcgaggaat gttctcatc gccgaaaac 
      ggcgtagcg aaagtgtcc gattttggg ttggcgcgg 
      aatgagatg aatcccatg tcggagtcg gcgaagttg 
      ccgatcaaa tggacggct cccgaggct ttgaagcga 
      aatatattt tcgaacaag tcggacatg tggagcttc 
      gggattctc ttatgggag atttactcc ttcggtaga 
      gttccgtac ccgagaata cctcttgcg gacgtggtg 
      aagcacgtg gagaaggga tacaagatg gaggctccg 
      gaaggctgt ccgtcggaa gtttacgaa attatgaga 
      caggcgtgg gatctgaat ccggacaaa agaccgacg 
      tttcaagac gtgaaggcg aaacttgca caattgaag 
      caacagact tcctga
    mRNA sequence
    • >TC010831 RA
      acattttcc aaaccaaaa tggctgttg ttggttaga 
      gattgacat tttaatgta tgtttttga aatttaaac 
      ccagtttgc tcataaaaa ataaatcag cgatgcttc 
      caaacgctc cagtcgaag tgttttaaa aaacttgtg 
      agtatgtgt tgagtcgaa gtggttgat tcttgagtg 
      atgaccagc caaaactcg ccatttcct cagaggtat 
      cagcacatt ttcgactac agtcaacaa acggacggt 
      caatgggac cccatgacc acctcacac ccacccaca 
      gttcctgcc aggggcatc aagaggagc acccctagc 
      gaagcgttc cgtccacat gatgaccct tcctggatg 
      gcgtcccaa gaagtggcg tcacaaatg gacaacagt 
      atcaagcac gaatgtctg agatccccg aataacatg 
      aacattagt tcagattgc aacaatttc aaaaatgtg 
      cgccatgac gtcaacgtt aaccatgtc cccagttta 
      atgcgaccg aattcgaaa ttcagtaca acaccgagt 
      tggtttcac ggcaacatt tcgcgcgag aacgccgaa 
      aatttgctg tcagcgcgc accgatggg ctgtttctc 
      gtgcgggaa tcaacgaat ttcccgggc gattacact 
      ctgtgtgtc tgctacgac aacaaagtc gagcattac 
      agagtgaag tcgaacgga ggtaaatta acaatcgac 
      gacgaggag ttttttgat aatctcgaa gacatgatc 
      gaacattac aaaaacgac gccgacggt ctctgtacg 
      aagttagtg aaagcgttg tgcaacgaa aagtcgaag 
      gaaaacgac gacttgtgc gtcatctct gaacaggag 
      ctcgagatc tgcgagcca atcggcaag ggcgagttt 
      tgcgaggtg atgctgggt aagtggaag aagaacaag 
      gtggcggtg aaggtgctg aaggactcg agcgaggca 
      gaggcaatt ttaatgaaa tcgctccac cacgagaat 
      ttggttaat ttgttaggc attgtgcgg aagaaagat 
      cagatttat ttggtgacg gagtatatg agtaaaggc 
      agtctagtt gattatttg cggtcgagg gggcgtttg 
      cacgtcacg aagaaggac cagatcaac tttgcgttt 
      gacacaagc tcgggtatg gagtatttg gagaaaatg 
      catgtggtg cacagggat ttagctgcg aggaatgtt 
      ctcatcgcc gaaaacggc gtagcgaaa gtgtccgat 
      tttgggttg gcgcggaat gagatgaat cccatgtcg 
      gagtcggcg aagttgccg atcaaatgg acggctccc 
      gaggctttg aagcgaaat atattttcg aacaagtcg 
      gacatgtgg agcttcggg attctctta tgggagatt 
      tactccttc ggtagagtt ccgtacccg agaatacct 
      cttgcggac gtggtgaag cacgtggag aagggatac 
      aagatggag gctccggaa ggctgtccg tcggaagtt 
      tacgaaatt atgagacag gcgtgggat ctgaatccg 
      gacaaaaga ccgacgttt caagacgtg aaggcgaaa 
      cttgcacaa ttgaagcaa cagacttcc tgatttagc 
      tctagtgta tattataga tagatgtat gcgtgtgag 
      tggatgtga atgcacttt gtgtgatcc ataatgtaa 
      tttcacttt tattgcaat tattattct aatcattac 
      tttagattt tttatttta actgtacaa ttatttctc 
      tgaatgtgg tgtgtctga ctagaaaat tgttgattt 
      atttccgta ctctttttg tacacagtt gatatttat 
      tttctcaaa tcatgtaaa tttgacgta cttaaattg 
      aatagtgtt caagtcagc ggaatcaaa agtaacgtc 
      gatttgttt tgctttgtt taggtgtaa gcgtactct 
      gatcggtca agtccaaat gtaattatt ttgtaaatg 
      tttttgttt tataacttt tctaaattg aaaatatat 
      tatttttac gttattgtt tagttttta acaattgga 
      gactatctt tgtagttta gcgttggct aaaaaggct 
      cagattttt ttgtaaatt tccgaacga tttatgtgt 
      aagttacaa tgagatgca tacgacaac aatcattaa 
      ataaaaagt gattaagaa t
    • >TC010831 RB
      acattttcc aaaccaaaa tggctgttg ttggttaga 
      gattgacat tttaatgta tgtttttga aatttaaac 
      ccagtttgc tcataaaaa ataaatcag cgatgcttc 
      caaacgctc cagtcgaag tgttttaaa aaacttgtg 
      agtatgtgt tgagtcgaa gtggttgat tcttgagtg 
      atgaccagc caaaactcg ccatttcct caggtgcgt 
      catttaaac ccatcgcgt attgaatac gtaaatcag 
      gttgggtcg ttgcagagg tatcagcac attttcgac 
      tacagtcaa caaacggac ggtcaatgg gaccccatg 
      accacctca cacccaccc acagttcct gccaggggc 
      atcaagagg agcacccct agcgaagcg ttccgtcca 
      catgatgac ccttcctgg atggcgtcc caagaagtg 
      gcgtcacaa atggacaac agtatcaag cacgaatgt 
      ctgagatcc ccgaataac atgaacatt agttcagat 
      tgcaacaat ttcaaaaat gtgcgccat gacgtcaac 
      gttaaccat gtccccagt ttaatgcga ccgaattcg 
      aaattcagt acaacaccg agttggttt cacggcaac 
      atttcgcgc gagaacgcc gaaaatttg ctgtcagcg 
      cgcaccgat gggctgttt ctcgtgcgg gaatcaacg 
      aatttcccg ggcgattac actctgtgt gtctgctac 
      gacaacaaa gtcgagcat tacagagtg aagtcgaac 
      ggaggtaaa ttaacaatc gacgacgag gagtttttt 
      gataatctc gaagacatg atcgaacat tacaaaaac 
      gacgccgac ggtctctgt acgaagtta gtgaaagcg 
      ttgtgcaac gaaaagtcg aaggaaaac gacgacttg 
      tgcgtcatc tctgaacag gagctcgag atctgcgag 
      ccaatcggc aagggcgag ttttgcgag gtgatgctg 
      ggtaagtgg aagaagaac aaggtggcg gtgaaggtg 
      ctgaaggac tcgagcgag gcagaggca attttaatg 
      aaatcgctc caccacgag aatttggtt aatttgtta 
      ggcattgtg cggaagaaa gatcagatt tatttggtg 
      acggagtat atgagtaaa ggcagtcta gttgattat 
      ttgcggtcg agggggcgt ttgcacgtc acgaagaag 
      gaccagatc aactttgcg tttgacaca agctcgggt 
      atggagtat ttggagaaa atgcatgtg gtgcacagg 
      gatttagct gcgaggaat gttctcatc gccgaaaac 
      ggcgtagcg aaagtgtcc gattttggg ttggcgcgg 
      aatgagatg aatcccatg tcggagtcg gcgaagttg 
      ccgatcaaa tggacggct cccgaggct ttgaagcga 
      aatatattt tcgaacaag tcggacatg tggagcttc 
      gggattctc ttatgggag atttactcc ttcggtaga 
      gttccgtac ccgagaata cctcttgcg gacgtggtg 
      aagcacgtg gagaaggga tacaagatg gaggctccg 
      gaaggctgt ccgtcggaa gtttacgaa attatgaga 
      caggcgtgg gatctgaat ccggacaaa agaccgacg 
      tttcaagac gtgaaggcg aaacttgca caattgaag 
      caacagact tcctgattt agctctagt gtatattat 
      agatagatg tatgcgtgt gagtggatg tgaatgcac 
      tttgtgtga tccataatg taatttcac ttttattgc 
      aattattat tctaatcat tactttaga ttttttatt 
      ttaactgta caattattt ctctgaatg tggtgtgtc 
      tgactagaa aattgttga tttatttcc gtactcttt 
      ttgtacaca gttgatatt tattttctc aaatcatgt 
      aaatttgac gtacttaaa ttgaatagt gttcaagtc 
      agcggaatc aaaagtaac gtcgatttg ttttgcttt 
      gtttaggtg taagcgtac tctgatcgg tcaagtcca 
      aatgtaatt attttgtaa atgtttttg ttttataac 
      ttttctaaa ttgaaaata tattatttt tacgttatt 
      gtttagttt ttaacaatt ggagactat ctttgtagt 
      ttagcgttg gctaaaaag gctcagatt tttttgtaa 
      atttccgaa cgatttatg tgtaagtta caatgagat 
      gcatacgac aacaatcat taaataaaa agtgattaa 
      gaat
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