General information | |||
TC010831 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 6113389..6116884 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC010831 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01742 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 5 (16 dpi)
pupal molt delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC010831 RA
MTSQNSPFP QRYQHIFDY SQQTDGQWD PMTTSHPPT VPARGIKRS TPSEAFRPH DDPSWMASQ EVASQMDNS IKHECLRSP NNMNISSDC NNFKNVRHD VNVNHVPSL MRPNSKFST TPSWFHGNI SRENAENLL SARTDGLFL VRESTNFPG DYTLCVCYD NKVEHYRVK SNGGKLTID DEEFFDNLE DMIEHYKND ADGLCTKLV KALCNEKSK ENDDLCVIS EQELEICEP IGKGEFCEV MLGKWKKNK VAVKVLKDS SEAEAILMK SLHHENLVN LLGIVRKKD QIYLVTEYM SKGSLVDYL RSRGRLHVT KKDQINFAF DTSSGMEYL EKMHVVHRD LAARNVLIA ENGVAKVSD FGLARNEMN PMSESAKLP IKWTAPEAL KRNIFSNKS DMWSFGILL WEIYSFGRV PYPRIPLAD VVKHVEKGY KMEAPEGCP SEVYEIMRQ AWDLNPDKR PTFQDVKAK LAQLKQQTS
- >TC010831 RB
MTSQNSPFP QVRHLNPSR IEYVNQVGS LQRYQHIFD YSQQTDGQW DPMTTSHPP TVPARGIKR STPSEAFRP HDDPSWMAS QEVASQMDN SIKHECLRS PNNMNISSD CNNFKNVRH DVNVNHVPS LMRPNSKFS TTPSWFHGN ISRENAENL LSARTDGLF LVRESTNFP GDYTLCVCY DNKVEHYRV KSNGGKLTI DDEEFFDNL EDMIEHYKN DADGLCTKL VKALCNEKS KENDDLCVI SEQELEICE PIGKGEFCE VMLGKWKKN KVAVKVLKD SSEAEAILM KSLHHENLV NLLGIVRKK DQIYLVTEY MSKGSLVDY LRSRGRLHV TKKDQINFA FDTSSGMEY LEKMHVVHR DLAARNVLI AENGVAKVS DFGLARNEM NPMSESAKL PIKWTAPEA LKRNIFSNK SDMWSFGIL LWEIYSFGR VPYPRIPLA DVVKHVEKG YKMEAPEGC PSEVYEIMR QAWDLNPDK RPTFQDVKA KLAQLKQQT S
- >TC010831 RA
atgaccagc caaaactcg ccatttcct cagaggtat cagcacatt ttcgactac agtcaacaa acggacggt caatgggac cccatgacc acctcacac ccacccaca gttcctgcc aggggcatc aagaggagc acccctagc gaagcgttc cgtccacat gatgaccct tcctggatg gcgtcccaa gaagtggcg tcacaaatg gacaacagt atcaagcac gaatgtctg agatccccg aataacatg aacattagt tcagattgc aacaatttc aaaaatgtg cgccatgac gtcaacgtt aaccatgtc cccagttta atgcgaccg aattcgaaa ttcagtaca acaccgagt tggtttcac ggcaacatt tcgcgcgag aacgccgaa aatttgctg tcagcgcgc accgatggg ctgtttctc gtgcgggaa tcaacgaat ttcccgggc gattacact ctgtgtgtc tgctacgac aacaaagtc gagcattac agagtgaag tcgaacgga ggtaaatta acaatcgac gacgaggag ttttttgat aatctcgaa gacatgatc gaacattac aaaaacgac gccgacggt ctctgtacg aagttagtg aaagcgttg tgcaacgaa aagtcgaag gaaaacgac gacttgtgc gtcatctct gaacaggag ctcgagatc tgcgagcca atcggcaag ggcgagttt tgcgaggtg atgctgggt aagtggaag aagaacaag gtggcggtg aaggtgctg aaggactcg agcgaggca gaggcaatt ttaatgaaa tcgctccac cacgagaat ttggttaat ttgttaggc attgtgcgg aagaaagat cagatttat ttggtgacg gagtatatg agtaaaggc agtctagtt gattatttg cggtcgagg gggcgtttg cacgtcacg aagaaggac cagatcaac tttgcgttt gacacaagc tcgggtatg gagtatttg gagaaaatg catgtggtg cacagggat ttagctgcg aggaatgtt ctcatcgcc gaaaacggc gtagcgaaa gtgtccgat tttgggttg gcgcggaat gagatgaat cccatgtcg gagtcggcg aagttgccg atcaaatgg acggctccc gaggctttg aagcgaaat atattttcg aacaagtcg gacatgtgg agcttcggg attctctta tgggagatt tactccttc ggtagagtt ccgtacccg agaatacct cttgcggac gtggtgaag cacgtggag aagggatac aagatggag gctccggaa ggctgtccg tcggaagtt tacgaaatt atgagacag gcgtgggat ctgaatccg gacaaaaga ccgacgttt caagacgtg aaggcgaaa cttgcacaa ttgaagcaa cagacttcc tga
- >TC010831 RB
atgaccagc caaaactcg ccatttcct caggtgcgt catttaaac ccatcgcgt attgaatac gtaaatcag gttgggtcg ttgcagagg tatcagcac attttcgac tacagtcaa caaacggac ggtcaatgg gaccccatg accacctca cacccaccc acagttcct gccaggggc atcaagagg agcacccct agcgaagcg ttccgtcca catgatgac ccttcctgg atggcgtcc caagaagtg gcgtcacaa atggacaac agtatcaag cacgaatgt ctgagatcc ccgaataac atgaacatt agttcagat tgcaacaat ttcaaaaat gtgcgccat gacgtcaac gttaaccat gtccccagt ttaatgcga ccgaattcg aaattcagt acaacaccg agttggttt cacggcaac atttcgcgc gagaacgcc gaaaatttg ctgtcagcg cgcaccgat gggctgttt ctcgtgcgg gaatcaacg aatttcccg ggcgattac actctgtgt gtctgctac gacaacaaa gtcgagcat tacagagtg aagtcgaac ggaggtaaa ttaacaatc gacgacgag gagtttttt gataatctc gaagacatg atcgaacat tacaaaaac gacgccgac ggtctctgt acgaagtta gtgaaagcg ttgtgcaac gaaaagtcg aaggaaaac gacgacttg tgcgtcatc tctgaacag gagctcgag atctgcgag ccaatcggc aagggcgag ttttgcgag gtgatgctg ggtaagtgg aagaagaac aaggtggcg gtgaaggtg ctgaaggac tcgagcgag gcagaggca attttaatg aaatcgctc caccacgag aatttggtt aatttgtta ggcattgtg cggaagaaa gatcagatt tatttggtg acggagtat atgagtaaa ggcagtcta gttgattat ttgcggtcg agggggcgt ttgcacgtc acgaagaag gaccagatc aactttgcg tttgacaca agctcgggt atggagtat ttggagaaa atgcatgtg gtgcacagg gatttagct gcgaggaat gttctcatc gccgaaaac ggcgtagcg aaagtgtcc gattttggg ttggcgcgg aatgagatg aatcccatg tcggagtcg gcgaagttg ccgatcaaa tggacggct cccgaggct ttgaagcga aatatattt tcgaacaag tcggacatg tggagcttc gggattctc ttatgggag atttactcc ttcggtaga gttccgtac ccgagaata cctcttgcg gacgtggtg aagcacgtg gagaaggga tacaagatg gaggctccg gaaggctgt ccgtcggaa gtttacgaa attatgaga caggcgtgg gatctgaat ccggacaaa agaccgacg tttcaagac gtgaaggcg aaacttgca caattgaag caacagact tcctga
- >TC010831 RA
acattttcc aaaccaaaa tggctgttg ttggttaga gattgacat tttaatgta tgtttttga aatttaaac ccagtttgc tcataaaaa ataaatcag cgatgcttc caaacgctc cagtcgaag tgttttaaa aaacttgtg agtatgtgt tgagtcgaa gtggttgat tcttgagtg atgaccagc caaaactcg ccatttcct cagaggtat cagcacatt ttcgactac agtcaacaa acggacggt caatgggac cccatgacc acctcacac ccacccaca gttcctgcc aggggcatc aagaggagc acccctagc gaagcgttc cgtccacat gatgaccct tcctggatg gcgtcccaa gaagtggcg tcacaaatg gacaacagt atcaagcac gaatgtctg agatccccg aataacatg aacattagt tcagattgc aacaatttc aaaaatgtg cgccatgac gtcaacgtt aaccatgtc cccagttta atgcgaccg aattcgaaa ttcagtaca acaccgagt tggtttcac ggcaacatt tcgcgcgag aacgccgaa aatttgctg tcagcgcgc accgatggg ctgtttctc gtgcgggaa tcaacgaat ttcccgggc gattacact ctgtgtgtc tgctacgac aacaaagtc gagcattac agagtgaag tcgaacgga ggtaaatta acaatcgac gacgaggag ttttttgat aatctcgaa gacatgatc gaacattac aaaaacgac gccgacggt ctctgtacg aagttagtg aaagcgttg tgcaacgaa aagtcgaag gaaaacgac gacttgtgc gtcatctct gaacaggag ctcgagatc tgcgagcca atcggcaag ggcgagttt tgcgaggtg atgctgggt aagtggaag aagaacaag gtggcggtg aaggtgctg aaggactcg agcgaggca gaggcaatt ttaatgaaa tcgctccac cacgagaat ttggttaat ttgttaggc attgtgcgg aagaaagat cagatttat ttggtgacg gagtatatg agtaaaggc agtctagtt gattatttg cggtcgagg gggcgtttg cacgtcacg aagaaggac cagatcaac tttgcgttt gacacaagc tcgggtatg gagtatttg gagaaaatg catgtggtg cacagggat ttagctgcg aggaatgtt ctcatcgcc gaaaacggc gtagcgaaa gtgtccgat tttgggttg gcgcggaat gagatgaat cccatgtcg gagtcggcg aagttgccg atcaaatgg acggctccc gaggctttg aagcgaaat atattttcg aacaagtcg gacatgtgg agcttcggg attctctta tgggagatt tactccttc ggtagagtt ccgtacccg agaatacct cttgcggac gtggtgaag cacgtggag aagggatac aagatggag gctccggaa ggctgtccg tcggaagtt tacgaaatt atgagacag gcgtgggat ctgaatccg gacaaaaga ccgacgttt caagacgtg aaggcgaaa cttgcacaa ttgaagcaa cagacttcc tgatttagc tctagtgta tattataga tagatgtat gcgtgtgag tggatgtga atgcacttt gtgtgatcc ataatgtaa tttcacttt tattgcaat tattattct aatcattac tttagattt tttatttta actgtacaa ttatttctc tgaatgtgg tgtgtctga ctagaaaat tgttgattt atttccgta ctctttttg tacacagtt gatatttat tttctcaaa tcatgtaaa tttgacgta cttaaattg aatagtgtt caagtcagc ggaatcaaa agtaacgtc gatttgttt tgctttgtt taggtgtaa gcgtactct gatcggtca agtccaaat gtaattatt ttgtaaatg tttttgttt tataacttt tctaaattg aaaatatat tatttttac gttattgtt tagttttta acaattgga gactatctt tgtagttta gcgttggct aaaaaggct cagattttt ttgtaaatt tccgaacga tttatgtgt aagttacaa tgagatgca tacgacaac aatcattaa ataaaaagt gattaagaa t
- >TC010831 RB
acattttcc aaaccaaaa tggctgttg ttggttaga gattgacat tttaatgta tgtttttga aatttaaac ccagtttgc tcataaaaa ataaatcag cgatgcttc caaacgctc cagtcgaag tgttttaaa aaacttgtg agtatgtgt tgagtcgaa gtggttgat tcttgagtg atgaccagc caaaactcg ccatttcct caggtgcgt catttaaac ccatcgcgt attgaatac gtaaatcag gttgggtcg ttgcagagg tatcagcac attttcgac tacagtcaa caaacggac ggtcaatgg gaccccatg accacctca cacccaccc acagttcct gccaggggc atcaagagg agcacccct agcgaagcg ttccgtcca catgatgac ccttcctgg atggcgtcc caagaagtg gcgtcacaa atggacaac agtatcaag cacgaatgt ctgagatcc ccgaataac atgaacatt agttcagat tgcaacaat ttcaaaaat gtgcgccat gacgtcaac gttaaccat gtccccagt ttaatgcga ccgaattcg aaattcagt acaacaccg agttggttt cacggcaac atttcgcgc gagaacgcc gaaaatttg ctgtcagcg cgcaccgat gggctgttt ctcgtgcgg gaatcaacg aatttcccg ggcgattac actctgtgt gtctgctac gacaacaaa gtcgagcat tacagagtg aagtcgaac ggaggtaaa ttaacaatc gacgacgag gagtttttt gataatctc gaagacatg atcgaacat tacaaaaac gacgccgac ggtctctgt acgaagtta gtgaaagcg ttgtgcaac gaaaagtcg aaggaaaac gacgacttg tgcgtcatc tctgaacag gagctcgag atctgcgag ccaatcggc aagggcgag ttttgcgag gtgatgctg ggtaagtgg aagaagaac aaggtggcg gtgaaggtg ctgaaggac tcgagcgag gcagaggca attttaatg aaatcgctc caccacgag aatttggtt aatttgtta ggcattgtg cggaagaaa gatcagatt tatttggtg acggagtat atgagtaaa ggcagtcta gttgattat ttgcggtcg agggggcgt ttgcacgtc acgaagaag gaccagatc aactttgcg tttgacaca agctcgggt atggagtat ttggagaaa atgcatgtg gtgcacagg gatttagct gcgaggaat gttctcatc gccgaaaac ggcgtagcg aaagtgtcc gattttggg ttggcgcgg aatgagatg aatcccatg tcggagtcg gcgaagttg ccgatcaaa tggacggct cccgaggct ttgaagcga aatatattt tcgaacaag tcggacatg tggagcttc gggattctc ttatgggag atttactcc ttcggtaga gttccgtac ccgagaata cctcttgcg gacgtggtg aagcacgtg gagaaggga tacaagatg gaggctccg gaaggctgt ccgtcggaa gtttacgaa attatgaga caggcgtgg gatctgaat ccggacaaa agaccgacg tttcaagac gtgaaggcg aaacttgca caattgaag caacagact tcctgattt agctctagt gtatattat agatagatg tatgcgtgt gagtggatg tgaatgcac tttgtgtga tccataatg taatttcac ttttattgc aattattat tctaatcat tactttaga ttttttatt ttaactgta caattattt ctctgaatg tggtgtgtc tgactagaa aattgttga tttatttcc gtactcttt ttgtacaca gttgatatt tattttctc aaatcatgt aaatttgac gtacttaaa ttgaatagt gttcaagtc agcggaatc aaaagtaac gtcgatttg ttttgcttt gtttaggtg taagcgtac tctgatcgg tcaagtcca aatgtaatt attttgtaa atgtttttg ttttataac ttttctaaa ttgaaaata tattatttt tacgttatt gtttagttt ttaacaatt ggagactat ctttgtagt ttagcgttg gctaaaaag gctcagatt tttttgtaa atttccgaa cgatttatg tgtaagtta caatgagat gcatacgac aacaatcat taaataaaa agtgattaa gaat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
