Status
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Gene information
General information
TC010831 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6113389..6116884
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010831
    iBeetle screen iB_01742

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection

    pupal molt delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    Pupal morphology
    antenna orientation bent - Number of animals showing the phenotype: 9

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010831 RA
      MTSQNSPFP QRYQHIFDY SQQTDGQWD PMTTSHPPT 
VPARGIKRS TPSEAFRPH DDPSWMASQ EVASQMDNS 
IKHECLRSP NNMNISSDC NNFKNVRHD VNVNHVPSL 
MRPNSKFST TPSWFHGNI SRENAENLL SARTDGLFL 
VRESTNFPG DYTLCVCYD NKVEHYRVK SNGGKLTID 
DEEFFDNLE DMIEHYKND ADGLCTKLV KALCNEKSK 
ENDDLCVIS EQELEICEP IGKGEFCEV MLGKWKKNK 
VAVKVLKDS SEAEAILMK SLHHENLVN LLGIVRKKD 
QIYLVTEYM SKGSLVDYL RSRGRLHVT KKDQINFAF 
DTSSGMEYL EKMHVVHRD LAARNVLIA ENGVAKVSD 
FGLARNEMN PMSESAKLP IKWTAPEAL KRNIFSNKS 
DMWSFGILL WEIYSFGRV PYPRIPLAD VVKHVEKGY 
KMEAPEGCP SEVYEIMRQ AWDLNPDKR PTFQDVKAK 
LAQLKQQTS
    • >TC010831 RB
      MTSQNSPFP QVRHLNPSR IEYVNQVGS LQRYQHIFD 
YSQQTDGQW DPMTTSHPP TVPARGIKR STPSEAFRP 
HDDPSWMAS QEVASQMDN SIKHECLRS PNNMNISSD 
CNNFKNVRH DVNVNHVPS LMRPNSKFS TTPSWFHGN 
ISRENAENL LSARTDGLF LVRESTNFP GDYTLCVCY 
DNKVEHYRV KSNGGKLTI DDEEFFDNL EDMIEHYKN 
DADGLCTKL VKALCNEKS KENDDLCVI SEQELEICE 
PIGKGEFCE VMLGKWKKN KVAVKVLKD SSEAEAILM 
KSLHHENLV NLLGIVRKK DQIYLVTEY MSKGSLVDY 
LRSRGRLHV TKKDQINFA FDTSSGMEY LEKMHVVHR 
DLAARNVLI AENGVAKVS DFGLARNEM NPMSESAKL 
PIKWTAPEA LKRNIFSNK SDMWSFGIL LWEIYSFGR 
VPYPRIPLA DVVKHVEKG YKMEAPEGC PSEVYEIMR 
QAWDLNPDK RPTFQDVKA KLAQLKQQT S
    CDS sequence
    • >TC010831 RA
      atgaccagc caaaactcg ccatttcct cagaggtat 
cagcacatt ttcgactac agtcaacaa acggacggt 
caatgggac cccatgacc acctcacac ccacccaca 
gttcctgcc aggggcatc aagaggagc acccctagc 
gaagcgttc cgtccacat gatgaccct tcctggatg 
gcgtcccaa gaagtggcg tcacaaatg gacaacagt 
atcaagcac gaatgtctg agatccccg aataacatg 
aacattagt tcagattgc aacaatttc aaaaatgtg 
cgccatgac gtcaacgtt aaccatgtc cccagttta 
atgcgaccg aattcgaaa ttcagtaca acaccgagt 
tggtttcac ggcaacatt tcgcgcgag aacgccgaa 
aatttgctg tcagcgcgc accgatggg ctgtttctc 
gtgcgggaa tcaacgaat ttcccgggc gattacact 
ctgtgtgtc tgctacgac aacaaagtc gagcattac 
agagtgaag tcgaacgga ggtaaatta acaatcgac 
gacgaggag ttttttgat aatctcgaa gacatgatc 
gaacattac aaaaacgac gccgacggt ctctgtacg 
aagttagtg aaagcgttg tgcaacgaa aagtcgaag 
gaaaacgac gacttgtgc gtcatctct gaacaggag 
ctcgagatc tgcgagcca atcggcaag ggcgagttt 
tgcgaggtg atgctgggt aagtggaag aagaacaag 
gtggcggtg aaggtgctg aaggactcg agcgaggca 
gaggcaatt ttaatgaaa tcgctccac cacgagaat 
ttggttaat ttgttaggc attgtgcgg aagaaagat 
cagatttat ttggtgacg gagtatatg agtaaaggc 
agtctagtt gattatttg cggtcgagg gggcgtttg 
cacgtcacg aagaaggac cagatcaac tttgcgttt 
gacacaagc tcgggtatg gagtatttg gagaaaatg 
catgtggtg cacagggat ttagctgcg aggaatgtt 
ctcatcgcc gaaaacggc gtagcgaaa gtgtccgat 
tttgggttg gcgcggaat gagatgaat cccatgtcg 
gagtcggcg aagttgccg atcaaatgg acggctccc 
gaggctttg aagcgaaat atattttcg aacaagtcg 
gacatgtgg agcttcggg attctctta tgggagatt 
tactccttc ggtagagtt ccgtacccg agaatacct 
cttgcggac gtggtgaag cacgtggag aagggatac 
aagatggag gctccggaa ggctgtccg tcggaagtt 
tacgaaatt atgagacag gcgtgggat ctgaatccg 
gacaaaaga ccgacgttt caagacgtg aaggcgaaa 
cttgcacaa ttgaagcaa cagacttcc tga
    • >TC010831 RB
      atgaccagc caaaactcg ccatttcct caggtgcgt 
catttaaac ccatcgcgt attgaatac gtaaatcag 
gttgggtcg ttgcagagg tatcagcac attttcgac 
tacagtcaa caaacggac ggtcaatgg gaccccatg 
accacctca cacccaccc acagttcct gccaggggc 
atcaagagg agcacccct agcgaagcg ttccgtcca 
catgatgac ccttcctgg atggcgtcc caagaagtg 
gcgtcacaa atggacaac agtatcaag cacgaatgt 
ctgagatcc ccgaataac atgaacatt agttcagat 
tgcaacaat ttcaaaaat gtgcgccat gacgtcaac 
gttaaccat gtccccagt ttaatgcga ccgaattcg 
aaattcagt acaacaccg agttggttt cacggcaac 
atttcgcgc gagaacgcc gaaaatttg ctgtcagcg 
cgcaccgat gggctgttt ctcgtgcgg gaatcaacg 
aatttcccg ggcgattac actctgtgt gtctgctac 
gacaacaaa gtcgagcat tacagagtg aagtcgaac 
ggaggtaaa ttaacaatc gacgacgag gagtttttt 
gataatctc gaagacatg atcgaacat tacaaaaac 
gacgccgac ggtctctgt acgaagtta gtgaaagcg 
ttgtgcaac gaaaagtcg aaggaaaac gacgacttg 
tgcgtcatc tctgaacag gagctcgag atctgcgag 
ccaatcggc aagggcgag ttttgcgag gtgatgctg 
ggtaagtgg aagaagaac aaggtggcg gtgaaggtg 
ctgaaggac tcgagcgag gcagaggca attttaatg 
aaatcgctc caccacgag aatttggtt aatttgtta 
ggcattgtg cggaagaaa gatcagatt tatttggtg 
acggagtat atgagtaaa ggcagtcta gttgattat 
ttgcggtcg agggggcgt ttgcacgtc acgaagaag 
gaccagatc aactttgcg tttgacaca agctcgggt 
atggagtat ttggagaaa atgcatgtg gtgcacagg 
gatttagct gcgaggaat gttctcatc gccgaaaac 
ggcgtagcg aaagtgtcc gattttggg ttggcgcgg 
aatgagatg aatcccatg tcggagtcg gcgaagttg 
ccgatcaaa tggacggct cccgaggct ttgaagcga 
aatatattt tcgaacaag tcggacatg tggagcttc 
gggattctc ttatgggag atttactcc ttcggtaga 
gttccgtac ccgagaata cctcttgcg gacgtggtg 
aagcacgtg gagaaggga tacaagatg gaggctccg 
gaaggctgt ccgtcggaa gtttacgaa attatgaga 
caggcgtgg gatctgaat ccggacaaa agaccgacg 
tttcaagac gtgaaggcg aaacttgca caattgaag 
caacagact tcctga
    mRNA sequence
    • >TC010831 RA
      acattttcc aaaccaaaa tggctgttg ttggttaga 
gattgacat tttaatgta tgtttttga aatttaaac 
ccagtttgc tcataaaaa ataaatcag cgatgcttc 
caaacgctc cagtcgaag tgttttaaa aaacttgtg 
agtatgtgt tgagtcgaa gtggttgat tcttgagtg 
atgaccagc caaaactcg ccatttcct cagaggtat 
cagcacatt ttcgactac agtcaacaa acggacggt 
caatgggac cccatgacc acctcacac ccacccaca 
gttcctgcc aggggcatc aagaggagc acccctagc 
gaagcgttc cgtccacat gatgaccct tcctggatg 
gcgtcccaa gaagtggcg tcacaaatg gacaacagt 
atcaagcac gaatgtctg agatccccg aataacatg 
aacattagt tcagattgc aacaatttc aaaaatgtg 
cgccatgac gtcaacgtt aaccatgtc cccagttta 
atgcgaccg aattcgaaa ttcagtaca acaccgagt 
tggtttcac ggcaacatt tcgcgcgag aacgccgaa 
aatttgctg tcagcgcgc accgatggg ctgtttctc 
gtgcgggaa tcaacgaat ttcccgggc gattacact 
ctgtgtgtc tgctacgac aacaaagtc gagcattac 
agagtgaag tcgaacgga ggtaaatta acaatcgac 
gacgaggag ttttttgat aatctcgaa gacatgatc 
gaacattac aaaaacgac gccgacggt ctctgtacg 
aagttagtg aaagcgttg tgcaacgaa aagtcgaag 
gaaaacgac gacttgtgc gtcatctct gaacaggag 
ctcgagatc tgcgagcca atcggcaag ggcgagttt 
tgcgaggtg atgctgggt aagtggaag aagaacaag 
gtggcggtg aaggtgctg aaggactcg agcgaggca 
gaggcaatt ttaatgaaa tcgctccac cacgagaat 
ttggttaat ttgttaggc attgtgcgg aagaaagat 
cagatttat ttggtgacg gagtatatg agtaaaggc 
agtctagtt gattatttg cggtcgagg gggcgtttg 
cacgtcacg aagaaggac cagatcaac tttgcgttt 
gacacaagc tcgggtatg gagtatttg gagaaaatg 
catgtggtg cacagggat ttagctgcg aggaatgtt 
ctcatcgcc gaaaacggc gtagcgaaa gtgtccgat 
tttgggttg gcgcggaat gagatgaat cccatgtcg 
gagtcggcg aagttgccg atcaaatgg acggctccc 
gaggctttg aagcgaaat atattttcg aacaagtcg 
gacatgtgg agcttcggg attctctta tgggagatt 
tactccttc ggtagagtt ccgtacccg agaatacct 
cttgcggac gtggtgaag cacgtggag aagggatac 
aagatggag gctccggaa ggctgtccg tcggaagtt 
tacgaaatt atgagacag gcgtgggat ctgaatccg 
gacaaaaga ccgacgttt caagacgtg aaggcgaaa 
cttgcacaa ttgaagcaa cagacttcc tgatttagc 
tctagtgta tattataga tagatgtat gcgtgtgag 
tggatgtga atgcacttt gtgtgatcc ataatgtaa 
tttcacttt tattgcaat tattattct aatcattac 
tttagattt tttatttta actgtacaa ttatttctc 
tgaatgtgg tgtgtctga ctagaaaat tgttgattt 
atttccgta ctctttttg tacacagtt gatatttat 
tttctcaaa tcatgtaaa tttgacgta cttaaattg 
aatagtgtt caagtcagc ggaatcaaa agtaacgtc 
gatttgttt tgctttgtt taggtgtaa gcgtactct 
gatcggtca agtccaaat gtaattatt ttgtaaatg 
tttttgttt tataacttt tctaaattg aaaatatat 
tatttttac gttattgtt tagttttta acaattgga 
gactatctt tgtagttta gcgttggct aaaaaggct 
cagattttt ttgtaaatt tccgaacga tttatgtgt 
aagttacaa tgagatgca tacgacaac aatcattaa 
ataaaaagt gattaagaa t
    • >TC010831 RB
      acattttcc aaaccaaaa tggctgttg ttggttaga 
gattgacat tttaatgta tgtttttga aatttaaac 
ccagtttgc tcataaaaa ataaatcag cgatgcttc 
caaacgctc cagtcgaag tgttttaaa aaacttgtg 
agtatgtgt tgagtcgaa gtggttgat tcttgagtg 
atgaccagc caaaactcg ccatttcct caggtgcgt 
catttaaac ccatcgcgt attgaatac gtaaatcag 
gttgggtcg ttgcagagg tatcagcac attttcgac 
tacagtcaa caaacggac ggtcaatgg gaccccatg 
accacctca cacccaccc acagttcct gccaggggc 
atcaagagg agcacccct agcgaagcg ttccgtcca 
catgatgac ccttcctgg atggcgtcc caagaagtg 
gcgtcacaa atggacaac agtatcaag cacgaatgt 
ctgagatcc ccgaataac atgaacatt agttcagat 
tgcaacaat ttcaaaaat gtgcgccat gacgtcaac 
gttaaccat gtccccagt ttaatgcga ccgaattcg 
aaattcagt acaacaccg agttggttt cacggcaac 
atttcgcgc gagaacgcc gaaaatttg ctgtcagcg 
cgcaccgat gggctgttt ctcgtgcgg gaatcaacg 
aatttcccg ggcgattac actctgtgt gtctgctac 
gacaacaaa gtcgagcat tacagagtg aagtcgaac 
ggaggtaaa ttaacaatc gacgacgag gagtttttt 
gataatctc gaagacatg atcgaacat tacaaaaac 
gacgccgac ggtctctgt acgaagtta gtgaaagcg 
ttgtgcaac gaaaagtcg aaggaaaac gacgacttg 
tgcgtcatc tctgaacag gagctcgag atctgcgag 
ccaatcggc aagggcgag ttttgcgag gtgatgctg 
ggtaagtgg aagaagaac aaggtggcg gtgaaggtg 
ctgaaggac tcgagcgag gcagaggca attttaatg 
aaatcgctc caccacgag aatttggtt aatttgtta 
ggcattgtg cggaagaaa gatcagatt tatttggtg 
acggagtat atgagtaaa ggcagtcta gttgattat 
ttgcggtcg agggggcgt ttgcacgtc acgaagaag 
gaccagatc aactttgcg tttgacaca agctcgggt 
atggagtat ttggagaaa atgcatgtg gtgcacagg 
gatttagct gcgaggaat gttctcatc gccgaaaac 
ggcgtagcg aaagtgtcc gattttggg ttggcgcgg 
aatgagatg aatcccatg tcggagtcg gcgaagttg 
ccgatcaaa tggacggct cccgaggct ttgaagcga 
aatatattt tcgaacaag tcggacatg tggagcttc 
gggattctc ttatgggag atttactcc ttcggtaga 
gttccgtac ccgagaata cctcttgcg gacgtggtg 
aagcacgtg gagaaggga tacaagatg gaggctccg 
gaaggctgt ccgtcggaa gtttacgaa attatgaga 
caggcgtgg gatctgaat ccggacaaa agaccgacg 
tttcaagac gtgaaggcg aaacttgca caattgaag 
caacagact tcctgattt agctctagt gtatattat 
agatagatg tatgcgtgt gagtggatg tgaatgcac 
tttgtgtga tccataatg taatttcac ttttattgc 
aattattat tctaatcat tactttaga ttttttatt 
ttaactgta caattattt ctctgaatg tggtgtgtc 
tgactagaa aattgttga tttatttcc gtactcttt 
ttgtacaca gttgatatt tattttctc aaatcatgt 
aaatttgac gtacttaaa ttgaatagt gttcaagtc 
agcggaatc aaaagtaac gtcgatttg ttttgcttt 
gtttaggtg taagcgtac tctgatcgg tcaagtcca 
aatgtaatt attttgtaa atgtttttg ttttataac 
ttttctaaa ttgaaaata tattatttt tacgttatt 
gtttagttt ttaacaatt ggagactat ctttgtagt 
ttagcgttg gctaaaaag gctcagatt tttttgtaa 
atttccgaa cgatttatg tgtaagtta caatgagat 
gcatacgac aacaatcat taaataaaa agtgattaa 
gaat
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