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Gene information
General information
TC010829 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6119927..6122234
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010829
    iBeetle screen iB_01741

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010829 RA
      MREIVHLQV GQCGNQIGS RFWHTISKE HGINPVGKY 
      VGDNDLQLE RIEVYFNEA ENANYVPRA VLVDLEPGT 
      MDMVRAGPL GTLFNPNNF VFAQSGAGN NWATGHYTE 
      GAEICDNVL DVIQHEVER CDCFQGFQF THSLGGGTG 
      SGFGTLLTS KVREEYPDR MVTSFSVVP SPKVSEVVL 
      EPYNATLSV QQLIENTDQ TQCIDNEAL YDICFNTLK 
      KSSPKYDDL NHLISLTMA GVTTCFRFP GQLNADLRK 
      LSVNMVPFP RLHFFVPGF APLTAPGSV AYRKLSVKD 
      LTEQMFSPR NIMAACNPA NGRYLTVAT IFRGPVSMK 
      EVDHVMFET QQKNSAYFV EWIPHNIQT AVCDISSPH 
      APISSTFIA NTTAIQELF KRIQEQFSA MFRRKAYVH 
      WYQAEGMDL QEFTEAESN LNDLISEYQ QYQDAEVDE 
      EYGDEDETE EDKFEEET
    CDS sequence
    • >TC010829 RA
      atgcgtgaa attgtgcat cttcaagtg ggtcaatgc 
      gggaaccaa attggttca cggttttgg catacgata 
      tcgaaagaa catggcatt aaccctgtt ggaaaatac 
      gtgggcgac aatgatctc caattggag cgaattgaa 
      gtgtacttt aacgaagcg gaaaacgcg aattacgtc 
      cctagggcg gtgctggtg gatctagaa cccggtacc 
      atggacatg gtgcgcgcc gggcctctc ggtaccctc 
      ttcaaccca aacaacttc gttttcgct cagtcgggt 
      gccgggaac aactgggcc accggtcac tacaccgaa 
      ggcgccgaa atttgcgac aacgtcttg gacgtgatc 
      caacacgag gttgagaga tgcgattgc ttccaaggg 
      ttccagttc acgcactct ctaggtggt ggtaccggc 
      tccggtttt ggcactttg ctcacttcg aaggtgcgt 
      gaggagtac cctgaccga atggtgaca agtttcagc 
      gtggtgcca tcaccgaaa gtctcggaa gtggttctg 
      gagccctac aatgcgacc ttgtccgtt cagcaactg 
      atcgaaaac accgatcag acgcagtgc attgacaat 
      gaagccctc tacgacatt tgcttcaac actttgaag 
      aaatcgtca ccgaagtac gacgatttg aaccatttg 
      atttccttg acaatggcg ggggtcacc acttgtttc 
      cggttcccg ggtcagttg aacgctgac ttgaggaaa 
      ctgtcggta aatatggta ccgtttcct cggctacac 
      ttcttcgtc cccggattt gcgccgctg acggctccg 
      ggaagtgtc gcttaccgg aaattaagc gtcaaagac 
      ttgactgaa cagatgttc agtccccgg aatatcatg 
      gcagcttgt aatccggca aacgggcga tatttgacc 
      gtcgctacc atcttccga ggacccgtt tcaatgaaa 
      gaggtagac catgtcatg tttgaaaca caacagaaa 
      aactcggcc tatttcgtc gagtggatc ccacataac 
      atccaaacg gcggtttgt gacatatcg tcgccacac 
      gcccccatt tcgtcaact ttcattgca aacaccaca 
      gccattcaa gaattattt aaacgcata caggaacag 
      ttctcagct atgttcaga cgcaaggcc tatgtgcat 
      tggtatcag gctgaaggc atggatttg caagaattc 
      acagaggct gaaagcaat ttgaatgac ttaatttcc 
      gaatatcag cagtatcag gatgcggaa gttgatgag 
      gagtatggg gatgaagac gaaacagaa gaggacaaa 
      tttgaagaa gaaacatga
    mRNA sequence
    • >TC010829 RA
      ttgtggtaa acagtccac tcgtaagtt ttgtgtaga 
      ataataggg ttgaaccgg cacgtaaaa atgcgtgaa 
      attgtgcat cttcaagtg ggtcaatgc gggaaccaa 
      attggttca cggttttgg catacgata tcgaaagaa 
      catggcatt aaccctgtt ggaaaatac gtgggcgac 
      aatgatctc caattggag cgaattgaa gtgtacttt 
      aacgaagcg gaaaacgcg aattacgtc cctagggcg 
      gtgctggtg gatctagaa cccggtacc atggacatg 
      gtgcgcgcc gggcctctc ggtaccctc ttcaaccca 
      aacaacttc gttttcgct cagtcgggt gccgggaac 
      aactgggcc accggtcac tacaccgaa ggcgccgaa 
      atttgcgac aacgtcttg gacgtgatc caacacgag 
      gttgagaga tgcgattgc ttccaaggg ttccagttc 
      acgcactct ctaggtggt ggtaccggc tccggtttt 
      ggcactttg ctcacttcg aaggtgcgt gaggagtac 
      cctgaccga atggtgaca agtttcagc gtggtgcca 
      tcaccgaaa gtctcggaa gtggttctg gagccctac 
      aatgcgacc ttgtccgtt cagcaactg atcgaaaac 
      accgatcag acgcagtgc attgacaat gaagccctc 
      tacgacatt tgcttcaac actttgaag aaatcgtca 
      ccgaagtac gacgatttg aaccatttg atttccttg 
      acaatggcg ggggtcacc acttgtttc cggttcccg 
      ggtcagttg aacgctgac ttgaggaaa ctgtcggta 
      aatatggta ccgtttcct cggctacac ttcttcgtc 
      cccggattt gcgccgctg acggctccg ggaagtgtc 
      gcttaccgg aaattaagc gtcaaagac ttgactgaa 
      cagatgttc agtccccgg aatatcatg gcagcttgt 
      aatccggca aacgggcga tatttgacc gtcgctacc 
      atcttccga ggacccgtt tcaatgaaa gaggtagac 
      catgtcatg tttgaaaca caacagaaa aactcggcc 
      tatttcgtc gagtggatc ccacataac atccaaacg 
      gcggtttgt gacatatcg tcgccacac gcccccatt 
      tcgtcaact ttcattgca aacaccaca gccattcaa 
      gaattattt aaacgcata caggaacag ttctcagct 
      atgttcaga cgcaaggcc tatgtgcat tggtatcag 
      gctgaaggc atggatttg caagaattc acagaggct 
      gaaagcaat ttgaatgac ttaatttcc gaatatcag 
      cagtatcag gatgcggaa gttgatgag gagtatggg 
      gatgaagac gaaacagaa gaggacaaa tttgaagaa 
      gaaacatga tttcaatac attgtcaat ttgttaaaa 
      aaaagaaat aaagacaaa tagagttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY